The Effects of Nanometer Titanium Dioxide on Tumor Cells and in Rats with Nasopharyngeal Carcinoma

A drug delivery system based on nanomaterials has demonstrated a powerful function in disease treatment. In this study, a titanium-dioxide-nanotube-based cisplatin (nano-TiO2-DDP) delivery system was designed, and its effects in rats with nasopharyngeal carcinoma (NPC) and on tumor cells were analyzed. First, we obtained electrochemistry anodic oxidation (EAO) for the preparation of Nnano-TiO2, which was adopted as the carrier of cisplatin (CDDP). Then, we used a scanning electron microscope (SEM) to characterize and study the surface morphology of nano-TiO2. At the cellular level, flow cytometry, MTT, and Transwell assays were performed to analyze the apoptosis, proliferation, and invasion of cells treated by nano-TiO2-DDP, respectively. At the animal level, a xenotransplantation model was established for evaluating tumor growth and changes in experimental animals after injection of nano-TiO2-DDP. As a result, nano-TiO2-DDP strongly suppressed the invasion and vitality of tumor cells, induced their apoptosis, and delivered DDP more efficiently than did systems without a nano-TiO2 structure. In addition, injected nano-TiO2-DDP strongly inhibited the growth of solid tumors in vivo. Therefore, we believe that nano-TiO2-DDP can effectively suppress the growth of NPC, and it is more efficient than conventional drugs.

Download Full-text

A medaka model of cancer allowing direct observation of transplanted tumor cells in vivo at a cellular-level resolution

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0903999106 ◽

2009 ◽

Vol 106 (33) ◽

pp. 13832-13837 ◽

Cited By ~ 11

Author(s):

S. Hasegawa ◽

K. Maruyama ◽

H. Takenaka ◽

T. Furukawa ◽

T. Saga

Keyword(s):

Tumor Cells ◽

Direct Observation ◽

Cellular Level ◽

Transplanted Tumor

Download Full-text

Precision Nanomedicine Vol. 1, Issue 3, Table of Contents

Precision Nanomedicine ◽

10.33218/prnano1(3).toc ◽

2018 ◽

Vol 1 (3) ◽

Cited By ~ 1

Keyword(s):

Pancreatic Cancer ◽

Cervical Cancer ◽

Tumor Cells ◽

Delivery System ◽

Complement Activation ◽

Sol Gel ◽

Therapeutic Vaccine ◽

Basic Research

Prec. Nanomed. 2018 Oct;1(3):183-193. BASIC RESEARCH From the Clinical Editor: The number of women affected by cervical cancer worldwide is very significant and the disease is associated with human papilloma virus (HPV) infection. Although the use of HPV vaccines has proven to be useful in disease protection, they only work in women who have never been infected by HPV previously. Thus, the development of a therapeutic vaccine that targets HPV-infected cells is needed for women who are already infected with the virus. In this study, the authors describe the use of a self-adjuvating polymer-based delivery system for the development of a therapeutic vaccine. Therefore, while efforts are progressing, vaccine candidates are still required against late stage cervical cancer via improving the vaccine delivery system. Authors demonstrate that the combination of polymer-based and liposome delivery systems may be effective without the use of additional adjuvant and with just a single dose immunization. This finding has potential importance for other cancer vaccines as well. Prec. Nanomed. 2018 Oct;1(3):173-182 POTENTIAL CLINICAL SIGNIFICANCE From the Clinical Editor: The treatment of triple-negative breast cancer is often difficult due to frequent resistance to doxorubicin. Using different nano-formulations based on sol-gel technology to encapsulate doxorubicin, the authors here showed enhanced dose-response metrics and tumor cell kill of these cancer cells due to an increased drug accumulation in the local tumor environment. This platform shows early promise in terms of eventual clinical translatability. Prec. Nanomed. 2018 Oct;1(3):194-207. BASIC RESEARCH From the Clinical Editor: Surgical resection remains the main treatment modality for pancreatic cancer. Thus, the ability to delineate the tumor accurately during operation is important to ensure all tumor cells are resected. Here, the authors describe the development of a multimodal imaging probe using nanospheres to target epithelial cells of pancreatic cancer. The specificity to target only tumor cells was clearly shown in both in-vitro and in-vivo experiments. This technology may provide a new fluorescence imaging technique to help the field of surgical oncology in the future. Prec. Nanomed. 2018 Oct;1(3):208-217. BASIC RESEARCH From the Clinical Editor: Preclinical characterization of nanotechnology-based products is essential for translating innovative applications into clinics. In addition to the innate immune system complement activation plays an important role in regulating the adaptive immune response. Undesirable activation of the complement system in response to new composites may lead to hypersensitivity reactions. The authors describe the importance of mouse strain selection for in vitro complement activation analysis addressing also the existence of inter- and intraspecies variability.

Download Full-text

DEVELOPMENT OF A PHOTOSENSITIZER DELIVERY SYSTEM FOR THERAPY AND DIAGNOSTICS IN ONCOLOGY

BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES ◽

10.37747/2312-640x-2020-18-124-126 ◽

2020 ◽

pp. 124-125

Author(s):

A.Semkina Semkina ◽

M.Abakumov Abakumov ◽

P.Ostroverkhov Ostroverkhov ◽

M. Grin

Keyword(s):

Photodynamic Therapy ◽

Physicochemical Properties ◽

Tumor Cells ◽

Therapeutic Effect ◽

Delivery System ◽

Biological Studies ◽

Kinetics Of ◽

Ct26 Tumor

In this work, the MNP-HSA-PEG-PS@4 complex was obtained and its physicochemical properties were studied. Biological studies have also been conducted. Namely, the ability of the drug to accumulate in CT26 tumor cells in vitro and the kinetics of drug accumulation in the tumor in vivo were studied. Then, the effectiveness of photodynamic therapy was studied under different conditions. The maximum therapeutic effect was achieved with irradiation at 1 hour after injection of the drug.

Download Full-text

LINC00210 as a miR-328-5p sponge promotes nasopharyngeal carcinoma tumorigenesis by activating NOTCH3 pathway

Bioscience Reports ◽

10.1042/bsr20181168 ◽

2018 ◽

Vol 38 (6) ◽

Cited By ~ 7

Author(s):

Shu Zhang ◽

Ping Li ◽

Lei Zhao ◽

Ling Xu

Keyword(s):

Cell Proliferation ◽

Nasopharyngeal Carcinoma ◽

Tumor Growth ◽

Signaling Pathway ◽

Untranslated Region ◽

Poor Prognosis ◽

Noncoding Rnas ◽

Proliferation And Invasion

As a kind of essential regulators, long noncoding RNAs (lncRNAs) have attracted a lot of attention in recent years. Nevertheless, the function of lncRNA in nasopharyngeal carcinoma (NPC) remains poorly understood. In the present study, we explained the role and mechanism of LINC00210 in NPC progression. We found that LINC00210 expression was up-regulated in NPC samples. Besides, its overexpression was positively correlated with NPC metastasis while predicting poor prognosis. Based on functional experiments, we revealed that LINC00210 contributed to NPC cell proliferation and invasion in vitro, and promotes tumor growth in vivo. Mechanistically, we identified that LINC00210 was located in the cytoplasm of NPC cells and served as the miR-328-5p sponge. Furthermore, we showed that miR-328-5p targets the 3′ untranslated region (3′-UTR) of NOTCH3. Through inhibiting miR-328-5p activity, LINC00210 promoted NOTCH3 expression in NPC, leading to activation of NOTCH3 signaling pathway. In conclusion, our study indicates LINC00210 promotes NPC progression through modulating proliferation and invasion.

Download Full-text

Overcoming drug resistance with functional mesoporous titanium dioxide nanoparticles combining targeting, drug delivery and photodynamic therapy

Journal of Materials Chemistry B ◽

10.1039/c8tb01810c ◽

2018 ◽

Vol 6 (46) ◽

pp. 7750-7759 ◽

Cited By ~ 11

Author(s):

Zhaoming Guo ◽

Kun Zheng ◽

Zhenquan Tan ◽

Ye Liu ◽

Ziyin Zhao ◽

...

Keyword(s):

Drug Delivery ◽

Drug Resistance ◽

Titanium Dioxide ◽

Photodynamic Therapy ◽

Tumor Cells ◽

Drug Delivery System ◽

Delivery System ◽

Titanium Dioxide Nanoparticles ◽

Nano Drug Delivery ◽

Targeting Strategy

We have designed a nano-drug delivery system ADH-1-HA-MTN, which can overcome the drug resistance of tumor cells based on an EMT cell targeting strategy in combination with PDT.

Download Full-text

Using C-doped TiO2 Nanoparticles as a Novel Sonosensitizer for Cancer Treatment

Antioxidants ◽

10.3390/antiox9090880 ◽

2020 ◽

Vol 9 (9) ◽

pp. 880 ◽

Cited By ~ 1

Author(s):

Chun-Chen Yang ◽

Chong-Xuan Wang ◽

Che-Yung Kuan ◽

Chih-Ying Chi ◽

Ching-Yun Chen ◽

...

Keyword(s):

Titanium Dioxide ◽

Tumor Cells ◽

Titanium Dioxide Nanoparticles ◽

Sol Gel ◽

Inorganic Materials ◽

Sonodynamic Therapy ◽

Carbon Doped ◽

Significant Difference ◽

Doped Titanium Dioxide

Sonodynamic therapy is an effective treatment for eliminating tumor cells by irradiating sonosentitizer in a patient’s body with higher penetration ultrasound and inducing the free radicals. Titanium dioxide has attracted the most attention due to its properties among many nanosensitizers. Hence, in this study, carbon doped titanium dioxide, one of inorganic materials, is applied to avoid the foregoing, and furthermore, carbon doped titanium dioxide is used to generate ROS under ultrasound irradiation to eliminate tumor cells. Spherical carbon doped titanium dioxide nanoparticles are synthesized by the sol-gel process. The forming of C-Ti-O bond may also induce defects in lattice which would be beneficial for the phenomenon of sonoluminescence to improve the effectiveness of sonodynamic therapy. By dint of DCFDA, WST-1, LDH and the Live/Dead test, carbon doped titanium dioxide nanoparticles are shown to be a biocompatible material which may induce ROS radicals to suppress the proliferation of 4T1 breast cancer cells under ultrasound treatment. From in vivo study, carbon doped titanium dioxide nanoparticles activated by ultrasound may inhibit the growth of the 4T1 tumor, and it showed a significant difference between sonodynamic therapy (SDT) and the other groups on the seventh day of the treatment.

Download Full-text

Influence of reduction-sensitive diselenide bonds and disulfide bonds on oligoethylenimine conjugates for gene delivery

Journal of Materials Chemistry B ◽

10.1039/c4tb00757c ◽

2014 ◽

Vol 2 (41) ◽

pp. 7210-7221 ◽

Cited By ~ 31

Author(s):

Dong Yue ◽

Gang Cheng ◽

Yiyan He ◽

Yu Nie ◽

Qian Jiang ◽

...

Keyword(s):

Gene Delivery ◽

System Design ◽

Tumor Cells ◽

Delivery System ◽

Disulfide Bonds ◽

Gene Delivery System

As a new reduction-sensitive linkage, diselenide bonds can also timely release DNA inside the tumor cells, while remaining constant outside the cells, implying its higher stability during the circulation process and great potential for in vivo gene delivery system design.

Download Full-text

Biomedical applications: Pitfalls in the practice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169626 ◽

1994 ◽

Vol 52 ◽

pp. 378-379

Author(s):

J. D. Shelburne ◽

Peter Ingram ◽

Victor L. Roggli ◽

Ann LeFurgey

Keyword(s):

Operating Room ◽

Liquid Nitrogen ◽

Lung Tissue ◽

Biomedical Applications ◽

Microprobe Analysis ◽

Tissue Sample ◽

Cellular Level ◽

Tissue Samples ◽

Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

Download Full-text