In Vitro Effects of Isobutyl Cyanoacrylate on Four Types of Bacteria

1971 ◽  
Vol 50 (6) ◽  
pp. 1557-1558 ◽  
Author(s):  
John Jandinski ◽  
Stephen Sonis
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Petri Dishes ◽  
In Vitro Effects ◽  
Streptococcus A ◽  
Polymer Application

Isobutyl cyanoacrylate produced varying degrees of growth inhibition when applied to freshly plated Petri dishes of Staphylococcus aureus, Gaffkya, Neisseria catarrhalis, and a-Streptococcus. A biproduct or diffusible substance produced by the polymer was implicated as the cause. Destruction of growing colonies was observed only with a-Streptococcus after polymer application. The mechanism for this was not determined.

scholarly journals Mechanism-Based Pharmacodynamic Models of Fluoroquinolone Resistance in Staphylococcus aureus

2006 ◽  
Vol 50 (9) ◽  
pp. 2957-2965 ◽  
Author(s):  
Philip Chung ◽  
Patrick J. McNamara ◽  
Jeffrey J. Campion ◽  
Martin E. Evans
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Fluoroquinolone Resistance ◽  
Pharmacodynamic Modeling ◽  
Common Mechanism ◽  
Maximum Effect ◽  
Parameter Estimates ◽  
High Density Culture ◽  
In Vitro System

ABSTRACT Pharmacodynamic modeling from earlier experiments in which two ciprofloxacin-susceptible Staphylococcus aureus strains and their corresponding resistant grlA mutants were exposed to a series of ciprofloxacin (J. J. Campion, P. J. McNamara, and M. E. Evans, Antimicrob. Agents Chemother. 49:209-219, 2005) and levofloxacin (J. J. Campion et al., Antimicrob. Agents Chemother. 49:2189-2199, 2005) pharmacokinetic profiles in an in vitro system indicated that the subpopulation-specific estimated maximal killing rate constants were similar for both agents, suggesting a common mechanism of action. We propose two novel pharmacodynamic models that assign mechanisms of action to fluoroquinolones (growth inhibition or death stimulation) and compare the abilities of these models and two other maximum effect models (net effect and MIC based) to describe and predict the changes in the population dynamics observed during our previous in vitro system experiments with ciprofloxacin. A high correlation between predicted and observed viable counts was observed for all models, but the best fits, as assessed by diagnostic tests, and the most precise parameter estimates were obtained with the growth inhibition and net effect models. All models, except the death stimulation model, correctly predicted that resistant subpopulations would not emerge when a high-density culture was exposed to a high initial concentration designed to rapidly eradicate low-level-resistant grlA mutants. Additional experiments are necessary to elucidate which of the proposed mechanistic models best characterizes the antibacterial effects of fluoroquinolone antimicrobial agents.

scholarly journals In vitro effects of glycyrrhetinic acid and hyaluronic acid on the growth of vulvovaginal Candida albicans and other yeasts

2017 ◽  
Vol 32 (4) ◽  
Author(s):  
Martina Stevan ◽  
Eleonora Fusato ◽  
Decio Armanini ◽  
Giulio Bertoloni ◽  
Francesco De Seta ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Hyaluronic Acid ◽  
Growth Inhibition ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Vulvovaginal Candidiasis ◽  
Glycyrrhetinic Acid ◽  
National Committee ◽  
In Vitro Effects

Aims. The present study aimed to test the in vitro activity against Candida albicans and non-albicans strains of 18-β glycyrrhetinic acid (18-β GA) and hyaluronic acid (HA), both alone and in combination. This antimicrobial activity was assessed using the National Committee for Clinical Laboratory Standards (NCCLS) method on Candida strains that were isolated from patients with recurrent vulvovaginal candidiasis (RVVC). Results. Our results demonstrate that the anti-Candida activity is independent from antifungal susceptibility level and the fact that the growth inhibition is stronger at acidic pH level makes the two drugs a promising biological alternative for the topical treatment of vulvovaginal candidiasis (VVC) and RVVC. Conclusions. Furthermore, the reduction of both budding cells formation and germ tube elongation, on mammalian cell monolayers, may explain the observed growth inhibition and suggest a decreased virulence, respectively.

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone 

scholarly journals Antimicrobial activity of calcium hydroxide associated with a new vehicle (Triethanolamine)

2016 ◽  
Vol 19 (1) ◽  
pp. 43
Author(s):  
Jaci Simi Junior ◽  
Ricardo Machado ◽  
Rafael Stiz ◽  
Cristiane Figueiredo ◽  
Luiz Pascoal Vansan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Olive Oil ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Culture Medium ◽  
Significance Level ◽  
Petri Dishes ◽  
Antimicrobial Effectiveness

<p><strong>Objective:</strong> The aim of this study was to evaluate the antimicrobial effectiveness of calcium hydroxide associated with a two conventional (anesthetic liquid and olive oil) and a new vehicle (Triethanolamine). <strong>Material and methods:</strong> Initially, microorganisms were collected from four upper incisors presenting unsatisfactory root canal treatments and persistent apical periodontitis from the same patient using sterile absorbent paper cones placed inside the root canal following initial access and root filling removal. Next, they were grown in a BHI culture medium for 24 h. Afterwards, they were placed in 10 Petri dishes with 3 holes, where different formulations of the medications studied were placed. After 48 and 72 h, readings were taken of the growth inhibition halos. <strong>Results:</strong> After using Kruskal-Wallis test with significance level of 1% (α = 0.01) it was observed that, calcium hydroxide associated with Triethanolamine, produced results similar to those produced by the anesthetic liquid. Both combinations were superior to the association with olive oil. <strong>Conclusion:</strong> Considering the results of this <em>in vitro</em> study, it is possible to consider that Triethanolamine can be a viable alternative to be used as a vehicle associated with the calcium hydroxide.</p><p><strong>Keywords:</strong> Vehicles; Calcium hydroxide; Endodontics.</p>

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

scholarly journals In vitro assessment of the bactericidal effect of low-power arsenium-gallium (AsGa) laser treatment

2012 ◽  
Vol 87 (4) ◽  
pp. 654-656 ◽  
Author(s):  
Adilvania Ferreira da Costa ◽  
Juvêncio César Lima de Assis
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Low Power ◽  
Laser Treatment ◽  
Bactericidal Effect ◽  
The Other ◽  
Bactericidal Action ◽  
In Vitro Assessment ◽  
Petri Dishes

The objective of the present study was to perform an in vitro evaluation of the bactericidal action of a low-power arsenium-gallium (AsGa) laser at a wavelength of 904nm and energy density of 6 J/cm². Ten petri dishes were seeded with Pseudomonas aeruginosa and another ten with Staphylococcus aureus. The dishes were then randomly divided into four groups with five plates in each group. Two groups were treated with AsGa laser once a day for 5 days, while the other two groups received no treatment. No halo of growth inhibition was found in any of the groups. It was therefore concluded that laser treatment (AsGa, 904nm, 6J/cm²) had no bactericidal effect.

Clofoctol-Induced Ultrastructural Changes In Staphylococcus Aureus

1999 ◽  
Vol 5 (S2) ◽  
pp. 1138-1139
Author(s):  
Beverly E. Maleeff ◽  
Stewart C. Pearson ◽  
David J. Payne ◽  
Timothy K. Hart
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Ultrastructural Changes ◽  
Upper Respiratory Tract ◽  
Butyl Phenol ◽  
Dividing Cells ◽  
Life Threatening ◽  
In Vitro Effects ◽  
Tract Infections

Clofoctol [2-(2,4-dichlorobenzyl)-4-(tetramethyl-l,l,3,3-butyl) phenol] is a synthetic antibacterial compound with activity against both Gram-positive1 and Gram-negative2 microorganisms. Although the specific mechanism of Clofoctol action has not been fully elucidated, it is reported to alter the permeability of the cytoplasmic membrane in Bacillus subtilis, and is used clinically to treat upper respiratory tract infections in humans. Staphylococcus aureus is a major human Grampositive pathogen that causes a variety of human diseases ranging from localized skin suppuration and food poisoning to life threatening presentations such as septicemia and endocarditis. Several clinical isolates of S. aureus have recently been identified that are resistant to all currently available antibacterial therapies. Consequently, the identification of new antimicrobial targets in this organism is of paramount importance. The purpose of this study was to investigate the in vitro effects of Clofoctol on S. aureus.Ultrastructural examination of S. aureus revealed a population of actively dividing cells.

scholarly journals In vitro effects of Staphylococcus aureus enterotoxin C3 on T cell activation, proliferation and cytokine production

2017 ◽  
Vol 16 (4) ◽  
pp. 4744-4750 ◽  
Author(s):  
Yixin Xie ◽  
Min Wang ◽  
Zhihui Dong ◽  
Huan Song ◽  
Lianping Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Cytokine Production ◽  
In Vitro Effects
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