scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone 

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

scholarly journals 25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Silvia P.N Keliat ◽  
Darniati Darniati ◽  
Abdul Harris ◽  
Erina Erina ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Final Conclusion ◽  
Distilled Water

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

2019 ◽  
Vol 4 (1) ◽  
pp. 278-287
Author(s):  
Lia Fikayuniar ◽  
Neni Sri Gunarti ◽  
Mellya Apriliani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus,    ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,  

scholarly journals Uji Aktivitas Ekstrak Etanolik Daun Kemangi (Ocimum sanctum L.) terhadap Staphylococcus aureus secara In Vitr

2020 ◽  
Vol 7 (1) ◽  
pp. 107
Author(s):  
Novia Ariani ◽  
Dwi Rizki Febrianti ◽  
Rakhmadhan Niah
Keyword(s):  
Staphylococcus Aureus ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Purposive Sampling ◽  
Extract Concentration

ABSTRAK Tanaman kemangi banyak dimanfaatkan oleh masyarakat untuk pengobatan infeksi khususnya bagian daun. Hal ini dikarenakan daun kemangi memiliki senyawa aktif seperti minyak atsiri, alkaloid, saponin, flavonoid, triterpenoid, steroid, tannin dan fenol yang dapat menghambat pertumbuhan bakteri. Tujuan penelitian ini dilakukan untuk mengetahui ada tidaknya aktivitas, mengetahui diameter zona hambat dan mengetahui klasifikasi kekuatan aktivitas daya hambat antibakteri ekstrak etanol daun kemangi. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi lubang sumuran dengan teknik pengambilan sampel adalah purposive sampling.  Konsentrasi ekstrak yang digunakan yaitu konsentrasi 100%, 80%, 60%, 40%, 20%, sedangkan untuk kontrol positif digunakan klindamisin 30µg, dan kontrol negatif yang digunakan etanol 96%. Hasil diameter zona hambat yang terbentuk diukur dengan jangka sorong. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kemangi memiliki aktivitas dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan diameter rata-rata yang didapat dari setiap perlakuan yaitu 100% (10,08 mm), 80% (8,10 mm), 60% (6,49 mm), 40% (4,29 mm), 20% (2,26 mm), dan sebagai klasifikasi kekuatan aktivitas daya hambat antibakteri yaitu pada konsentrasi 100% kuat, 80%-60% sedang dan 40%-20% lemah. Kata Kunci : Daun kemangi, Ekstrak, Difusi, Staphylooccus aureus  ABSTRACT Part of the basil plant (Ocimum sanctum L.) that widely used by people for treatment of infections is basil leaves. This is because basil leaves have active compounds such as essential oils, alkaloids, saponins, flavonoids, triterpenoids, steroids, tannins and phenols which can inhibit bacterial growth. This research aimed to find out the presence or absence of activity, to determine the diameter of the inhibitory zone and the classification of antibacterial mention against what the name of bacterial is activity of ethanol extract of basil leaves. The type of this research is experimental research with a well diffusion method with sampling technique is purposive sampling. The concentration of extracts used were concentrations of 100%, 80%, 60%, 40%, 20%, while as positive control is  clindamycin 30µg, and the negative control used 96% ethanol. The resulting diameter of the inhibition zone is measured by the calipers.  The results showed that the ethanol extract of basil leaves had an activity in inhibiting the growth of Staphylococcus aureus bacteria with an average diameter obtained from each treatment that was 100% (10,08mm); 80% (8,10mm); 60% (6,49mm); 40% (4,29mm); 20% (2,26mm), and as the antibacterial activity classification, that were strong in 100% of extract concentration, medium in 60-80% of extract concentration, and weak in 20-40% of extract concentration. Keywords : Basil leaf, Extract, Diffusion, Staphylooccus aureus

scholarly journals In Vitro Antimicrobial Activity of Bawang Batak Bulbs (Allium Chinense G. Don) Against Methicillin-Resistant Staphylococcus Aureus

2019 ◽  
Vol 2 (2) ◽  
pp. 67-71
Author(s):  
Tiasarah Aretha ◽  
Nelva Karmila Jusuf
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Alternative Therapy ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Allium Chinense ◽  
Concentration Levels

Introduction: Staphylococcus aureus infection can cause pyoderma. These days there is a shift in Staphylococcus aureus sensitivity to antibiotics that is marked by the existence of Methicillin-Resistant Staphyloccus aureus, so alternative therapy that is herbal medicine might be needed. There are a lot of medicinal plants in Indonesia, but until today the usage is still minimum. One of the plant genus that is already being used is allium that had been known to have antimicrobial activity. The genus allium that is consumed a lot by Indonesians especially people of North Sumatra is bawang batak (Allium chinense G.Don.). This study is conducted to assess the antimicrobial activity of bawang batak bulbs in several concentrations to MRSA. Methods: This research is an experimental research with agar diffusion method. The extracts were divided into six groups: extracts in concentration level of 6.25%, 12.5%, 25%, 50%, 100% and a negative control (DMSO). The sample size is four per group with total sample of 24. Results: The results showed that the inhibition of bacterial growth occured at the concentration levels of 50% and 100% with inhibition zone diameters of 8.695 mm and 10.545 mm respectively. Extracts with concentration levels of 25%, 12.5% and 6.25% did not show any inhibition. Conclusion: Bawang batak bulbs extract can be used to inhibit the growth of MRSA

scholarly journals In vitro Inhibitory Activity of Ethanolic Fruit Extract from Averrhoa bilimbi L. against Streptococcus pyogenes Bacteria

2019 ◽  
Vol 3 (1) ◽  
pp. 13-17
Author(s):  
Puspita Dewi ◽  
Gusti Ayu Ratih ◽  
Burhannuddin Burhannuddin ◽  
Gede Sudarmanto
Keyword(s):  
Streptococcus Pyogenes ◽  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Respiratory Infections ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Significant Difference

Averrhoa bilimbi L., popularly known as belimbing wuluh, is a common plant used as traditional medicine and cooking ingredients in Indonesia. Averrhoa bilimbi fruit contains many active substances as antibacterial such as tannin, formic acid, flavonoids, saponins, peroxides, and triterpenoids. Streptococcus pyogenes is a pathogenic bacteria that can cause respiratory infections. This study was carried out to investigate the inhibitory activity of Averrhoa bilimbi L. fruit extract at various concentrations on the growth of Streptococcus pyogenes. This study was a true experimental with post test only control design. The inhibitory activity was assessed by the KirbyBauer’s disc diffusion method with four different extract concentrations (20%, 40%, 60%, 80%), 96% EtOH as negative control, and Amoxicillin antibiotic as positif control. The EtOH extract showed the mean diameter of inhibition zone of 20% extract was 11.43 mm (strong), 40% extract was 19.13 mm (strong), 60% extract was 24.58 mm (very strong), 80% extract was 30.99 mm (very strong), and the negative control (96% EtOH) was 0.00 mm. Least Significant Difference test showed there were significant different of inhibition zone in each extract concentrations of Averrhoa bilimbi L. against Streptococcus pyogenes with the strongest inhibitory activity is 80% extract concentration. Keywords: Averrhoa bilimbi, Inhibitory activity, Streptococcus pyogenes

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

2018 ◽  
Vol 9 (3) ◽  
pp. 336
Author(s):  
Ni Luh Budi Artaningsih ◽  
Nur Habibah ◽  
Mastra Nyoman
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Gliricidia Sepium ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone

<p><em>Streptococcus mutans</em> is a positive gram bacteria which cause dental caries. From the several previous studies, Gamal leaf has been predicted as the antibacterial agent because of their active substance such as tannins, alkaloids, saponins, and flavonoids. The objective of the study was to know the antibacterial activity of ethanol extract of Gamal leaf (<em>Gliricidia sepium</em>) for <em>Streptococcus mutans</em> in various concentrations. This study was a true experimental with post-test only control design. The determination of antibacterial activity in this study was conducted by using Kirby Bauer disk diffusion method with the various concentrations, there were 40, 50, 60, 70 and 80%. The positive and negative controls in this study were 30 µg disk diffusion of chloramphenicol and the 96% of ethanol. The average inhibition of zone diameter in the concentrations of 40, 50, 60, 70 and 80% were 11.3, 12.3, 13.4, 15.3 and 19.2 mm, respectively. One Way ANOVA statistical analysis showed that the value of p was 0.000, so there was a difference of growth inhibition zone of <em>Streptococcus mutans</em> in various concentrations of ethanol extract of Gamal leaf (<em>Gliricidia sepium</em>). Based on the result, it can be concluded that the ethanol extract of Gamal leaf (<em>Gliricidia sepium</em>) have strong antibacterial activity, especially to <em>Streptococcus mutans</em> bacteria.</p>

scholarly journals The Antibacterial Effect of Ethanol Edamame Seeds (Glycine Max (L) Merril) Extract to E.coli Bacteria

2020 ◽  
Vol 6 (3) ◽  
pp. 137
Author(s):  
Diayu Putri Akhita ◽  
Edy Junaidi ◽  
Septa Surya Wahyudi
Keyword(s):  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
The Times

Abstract Infectious diseases can occur in all parts of the body. One of the causes infection in humans is Eschericiae coli bacteria. Eschericiae coli is a rod-shaped bacteria, a gram negative bacteria, facultative aerobics and classified family member of Enterobacteriaceae from the Gammaproteobacteria class. Along the times, E.coli bacteria have resistent to some antibiotics. So we need a new alternative. There is a antibacterial substance in the isoflavon group contained in edamame. Genistein is a main isoflavon in edamame that have antiinflammation, antibacterial, and antioxidant effects. The purpose of this study was to determine is there any antibacterial effects in ethanol edamame seeds extract to E.coli bacteria. This study used a true experimental research design in vitro with a post test only control group design.  The average diameter results of the inhibition zone were analyzed using the Kruskal-Wallis method and obtained p = 0.001 which means there are significant differences in at least two groups. After that, the Mann Whitney post hoc test was conducted and a significant difference was found in the positive and negative control groups for all groups but there was no difference in the treatment group, both groups K1, K2 and K3 for all groups. Keywords : Edamame, Antibacterial, E.coli

scholarly journals Evaluating the Effects of Peracetic Acid and Chlorine Dioxide Disinfectants on Staphylococcus Aureus Isolated from Surfaces of NICU

2019 ◽  
Author(s):  
Mina Usefi ◽  
Hengameh Zandi ◽  
Sara Jambarsang ◽  
Mehdi Mokhtari ◽  
Mahmood Noori Shadkam
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Chlorine Dioxide ◽  
Peracetic Acid ◽  
Antimicrobial Agents ◽  
Inhibition Zone ◽  
Health Care Facilities ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Growth Inhibition Zone

Introduction: Today, disinfection of surfaces by using antimicrobial agents is critical for the prevention and control of pathogens and reduction of infection in hospital. The aim of this study was to investigate and compare the effects of two disinfectants against Staphylococcus aureus isolated from the NICU of Shahid Sadoughi Hospital of Yazd in 2017. Materials and Methods: In this descriptive cross sectional study, bacterial culture of samples collected from different surfaces of the NICU and S. aureus isolates were identified using conventional biochemical tests. Peracetic acid and chlorine dioxide various concentrations were used as disinfectants. Their effects against Staphylococcus aureus were determined by Standard disc diffusion method. Data were analyzed by linear mixed models in SPSS version 23. Results: 39.39% of samples were found to be S. aureus infected. The mean diameter of growth inhibition zone for peracetic acid 0.1% was significantly lower than that for peracetic acid 0.2%, and peracetic acid 0.1% was significantly higher than that for chlorine dioxide (P < 0.001). The comparison of growth inhibition zone diameters for peracetic acid 0.1% and chlorine dioxide disinfectants showed that the average diameter of the inhibition zone created by peracetic acid 0.1% was significantly higher than that created by chlorine dioxide. The most effective disinfectant on S. aureus strains isolated was peracetic acid 0.2% and the least effective disinfectant was chlorine dioxide. Conclusion: In health care facilities with S. aureus infection, peracetic acid 0.2% can be used effectively to reduce nosocomial infection rate.

scholarly journals UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP ISOLAT BAKTERI Escherichia coli JAJANAN CILOK SECARA IN VITRO DENGAN METODE DIFUSI

2017 ◽  
Vol 11 (1) ◽  
Author(s):  
Fadel Abima ◽  
Meiskha Bahar ◽  
Aulia Chairani
Keyword(s):  
Escherichia Coli ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Base Layer ◽  
Diffusion Method ◽  
Major Health Problem ◽  
E Coli ◽  
Growth Inhibition Zone ◽  
Significant Difference

Diarrhea is still one of the world’s major health problem especially in developing country. Foods and beverages contaminated by microorganism become the risk factor of diarrhea, including Escherichia coli (E. coli). One of the curative effort that can be done is to utilise the secondary metabolite compounds contained in binahong leaf (Anredera cordifolia (Ten.) Steenis). This study used true experimental method. The sample was binahong leaf extract with concentration of 25%, 50, and 75% using diffusion method (Kirby-Bauer) on doublelayered Mueller Hinton Agar (MHA) (base layer and seed layer) in order to measure the growth inhibition zone around the cylinder plate. The amount of population on this study was 24 isolations of E. coli. The repetition of each treatment group was counted by Federer formula. The result analyzed using Kruskal-Wallis test and showed that there was difference on binahong leaf extract effectiveness (p < 0,05), as well as on post hoc analysis using MannWhitney test showed that there was significant difference at concentration of 25%,50%, and 75%. (p < 0,001). This shows that there is a significant differences in the concentration of binahong leaf extract.

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