scholarly journals Microbiological monitoring of heater-cooler unit to keep free of Mycobacterium chimaera infection

Perfusion ◽  
2018 ◽  
Vol 34 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Tanee Chan ◽  
Moi Lin Ling ◽  
Siew Yan Teng ◽  
Kit Yi Chiu ◽  
Esther Magdalane James
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Total Coliform ◽  
Colony Count ◽  
Microbiological Contamination ◽  
Strict Adherence ◽  
Coliform Count ◽  
Microbiological Monitoring ◽  
Instructions For Use ◽  
Cleaning And Disinfection

Introduction: The association of Mycobacterium chimaera infection in patients undergoing cardiopulmonary bypass (CPB) with the use of heater-cooler units (HCU) has been reported in various literature. We described microbiological monitoring and the extent of microbiological contamination of HCUs utilized in our centre and strategies employed to reduce the high microbial load. Methods: Since August 2016, we have been following the new Instructions for Use from the manufacturer for the cleaning and disinfection of three units of Stöckert 3T and four units of Stöckert 1T HCU at the National Heart Centre Singapore. Microbiological monitoring began in January 2017 and included acid-fast bacilli (AFB) culture, Pseudomonas aeruginosa, total colony and total coliform count. Methods, such as increasing disinfection frequency and making the HCU inactive by keeping it empty in storage, were used to reduce the high colony count. Results: All three units of Stöckert 3T and two units of Stöckert 1T were contaminated with Mycobacterium chimaera. Pseudomonas aeruginosa and total coliform count were consistently <1 colony-forming unit (CFU)/100 mL in every water sample of each HCU. High colony counts were encountered initially in all units. Step-up frequency of disinfection was found to be not as effective as keeping the HCU inactive in bringing the total colony count to an acceptable level. Conclusions: All monitoring and maintenance measures of HCUs need to be established and maintained to mitigate potential infection risks to patients. Strict adherence to all cleaning and disinfection processes and keeping the HCU inactive maintained the water quality of the HCU at acceptable levels.

scholarly journals Microbiological monitoring in two areas with different levels of conservation in the mangroves of an Ecological Station, Vitoria, ES

2016 ◽  
Vol 38 (3) ◽  
pp. 333 ◽  
Author(s):  
Juliano De Oliveira Barbirato ◽  
Juliana Melo Conceição ◽  
Dandara Silva Cabral ◽  
André Luiz Paier Barroso ◽  
Daniel Basílio Zandonadi ◽  
...  
Keyword(s):  
Environmental Quality ◽  
Human Population ◽  
Interstitial Water ◽  
Total Coliform ◽  
Environmental Characteristics ◽  
Microbiological Monitoring ◽  
Strong Indicator ◽  
Different Levels

Mangroves are classified as permanent preservation areas and regarded as natural nurseries. However, they have suffered several anthropogenic stresses, resulting in their decline. In the light of that, comes the importance of researching their environmental characteristics and revealing possible factors that have led to the degradation of this important ecosystem. The aim of this study was to evaluate the environmental quality of different areas in the mangroves of Ilha do Lameirão Ecological Station through microbiological analyzes of sediment and interstitial water along ten (10) sites, distributed in two areas with different conservation levels (Canal dos Escravos (CE) and Maria Ortiz (MO)) between 2010 and 2012. The microbiological analyzes revealed that MO region, in all seasons of the year, achieved total coliform and thermo-tolerant coliform values above those permitted by the CONAMA Resolution 357/05, fitting the Class 2 conservation standard. The presence of high levels of total and thermo-tolerant coliforms in MO is a strong indicator of impacts originated from the human population and, consequently, the decline of the mangrove itself and the health of human communities surrounding that area. 

Assessment of the microbiological quality of natural mineral waters according to the manufacturing time of 20 L returnable packs in Brazil

2020 ◽  
Vol 367 (15) ◽  
Author(s):  
Isabelle da Silva Luz ◽  
Luiza Vasconcellos ◽  
Valéria de Mello Medeiros ◽  
Catia Aparecida Chaia Miranda ◽  
Carla de Oliveira Rosas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Microbiological Quality ◽  
Human Adenovirus ◽  
Mineral Waters ◽  
Microbiological Contamination ◽  
Natural Mineral ◽  
Norovirus Gii ◽  
Manufacturing Time ◽  
Sequence Types

ABSTRACT This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, 99 water samples from 33 lots (n = 3/batch) of 15 brands, obtained from packs with three intervals of Tm, were analyzed. Total coliforms (16.2%), Pseudomonas aeruginosa (9.9%), sulphite-reducing Clostridium (5.0%) and Escherichia coli (2.0%) were detected but enterococci and norovirus GII not. Regarding brands, 11 (73.3%) presented unsatisfactory results for at least one of the lots analyzed. Pseudomonas aeruginosa analysis revealed six sequence types and strains were susceptible to all antibiotics tested and were able to produce biofilms. Human adenovirus (4) and norovirus GI (9) were also identified in nine samples randomly selected. Natural mineral waters commercialized in 20 L packs with Tm ≥ 2 years presented more microbiological contamination (P ≤ 0.012) than ones with a Tm of 0–1 year or a Tm of 1–2 years. These results suggest that the validity period of reusable 20 L packs should be reduced or that they can no longer be reused.

scholarly journals Public Health Safety in Relation to Microbiological Quality of Freshly Drawn Cow’s Milk in Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 231-236
Author(s):  
R Khaton ◽  
MA Hasnat ◽  
S Rahman ◽  
MM Rahman
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Viable Count ◽  
Microbial Quality ◽  
Total Viable Count ◽  
Coliform Count ◽  
Milk Samples

The aim of the present study was to determine the microbial quality of freshly drawn cow’s milk belonging to the arsenic affected and non-affected areas. In the present work 25 milk sample were collected from five different arsenic affected and non-affected areas. The analysis comprised enumeration of total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The highest total viable count, total coliform count and total staphylococcus count were log 5.894 ± 0.221, log 2.832± 0.129 and log 2.898 ± 0.162 respectively. Staphylococcus spp, Escherichia coli, Pseudomonas spp and Bacillus spp were isolated from the milk samples. Among them Escherichia coli were isolated from the maximum milk samples. It is found that the level of microbial quality in terms of TVC, TCC and TSC were high in arsenic affected areas than arsenic non-affected area. It also found that TVC and TCC were positively correlated with each other. This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples and it is necessary to improve the hygienic practices required for the handling and processing of milk.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21297 Bangl. J. Vet. Med. (2014). 12 (2): 231-236 

Risk of bacillus cereus and pseudomonas aeruginosa nosocomial infections in a burns centre: the microbiological monitoring of water supplies for a preventive strategy

1995 ◽  
Vol 31 (5-6) ◽  
pp. 37-40
Author(s):  
L. Valentino ◽  
M. V. Torregrossa
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Nosocomial Infections ◽  
Preventive Strategy ◽  
Water Supplies ◽  
Microbiological Monitoring ◽  
Quality Of Water

Opportunist bacteria are a potentially very serious problem in hospital burns units, particularly where the quantity and quality of water supplies may not be reliable, and patients are especially vulnerable. A programme of routine microbiological monitoring is described which minimises the risk to patients.

scholarly journals The effect of container-biofilm on the microbiological quality of water used from plastic household containers

2003 ◽  
Vol 1 (3) ◽  
pp. 101-108 ◽  
Author(s):  
P. Jagals ◽  
C. Jagals ◽  
T. C. Bokako
Keyword(s):  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Human Consumption ◽  
Microbiological Contamination ◽  
Supply Source ◽  
E Coli ◽  
Quality Of Water ◽  
Storage Containers

Studies in Southern Africa have shown that even when microbiologically safe water is supplied to developing communities at communal standpipes, contamination by high numbers of pathogenic microorganisms may occur during the processes of fetching water from the supply source and storage during use at home, rendering such waters unsafe for human consumption. This study investigated the occurrence of biofilm in PVC storage containers as one possible reason for this deterioration, using heterotrophic bacteria and total coliform counts as well as turbidity as indicators. A second objective was to determine whether biofilm in water-storage containers could contribute to hazardous microbiological contamination indicated by Escherichia coli and Clostridium perfringens. Results indicated that increased microbiological contamination is associated with biofilm. The biofilm harbours heterotrophic bacteria, total coliforms and C. perfringens. E. coli could not be associated directly with the levels of biofilm in containers but rather appears to be introduced intermittently from the ambient domestic environment. When dislodged with the biofilm, these bacteria contributed substantially to the deterioration of the microbiological quality of supplied water stored in plastic containers.

scholarly journals Assessment of bacteriological quality and Escherichia coli O157: H7 in ready-to-eat street foods

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 98-105
Author(s):  
S.R. Latchumaya ◽  
M.N.S.S. Hameed ◽  
P. Arkappan ◽  
R.J. Sreedharam ◽  
P. Sandrasaigaran
Keyword(s):  
Microbiological Quality ◽  
Total Coliform ◽  
Colony Count ◽  
Laboratory Service ◽  
The Public ◽  
E Coli ◽  
Bacteriological Quality ◽  
Legal Enforcement ◽  
Night Markets

Ready-to-eat (RTE) street foods are usually prepared well in advance (4-8 hrs) before the sale. Owning to minimal cooking procedures, cross-contaminations, and abused holding temperature and time, there is a high probability for deteriorating microbiological quality in these foods. Thus, this study was aimed to assess the bacteriological quality of RTE foods sampled from night markets and street stalls based on the guidelines provided by the Public Health Laboratory Service. A total of fifty samples (category 2, 3 and 4) were evaluated for aerobic colony count, total coliform, and E. coli. The pathogenic strain E. coli O157: H7 were examined using the multiplex PCR technique. It was noticed that category 3 and 4 RTE street foods were largely unsatisfactory for the coliform (>104 CFU/ mL) and E. coli count (>100 CFU/mL). In opposite, category 2 RTE street foods were unsatisfactory for the aerobic colony count (>105 CFU/mL) and E. coli (>100 CFU/ mL). However, there was no E. coli O157: H7 or Shiga-toxin producing bacteria reported in this study. The statistical analysis showed that in overall, category 3 and 4 RTE street foods were significantly at worsening bacteriological quality compared to category 2. Strict legal enforcement and amending the existing rules are needed to improve the quality of RTE street foods sold in the night markets and street stalls.

scholarly journals Physico-Chemical and Bacteriological Quality of Drinking Water Sources in Calabar Municipality, Nigeria

2019 ◽  
pp. 1-22
Author(s):  
B. E. Agbo ◽  
A. V. Ogar ◽  
U. L. Akpan ◽  
C. I. Mboto
Keyword(s):  
Drinking Water ◽  
Dissolved Oxygen ◽  
Total Coliform ◽  
Water Sources ◽  
Faecal Coliform ◽  
Total Hardness ◽  
Coliform Count ◽  
Physico Chemical ◽  
The Mean

This study evaluated the physico-chemical and the bacteriological quality of five different sources of drinking water in Calabar metropolis, Nigeria, to give a fair geographical representative of the town and to contribute to our understanding of the quality of drinking water in the metropolis. The physico-chemical characteristics such as pH, temperature, turbidity, conductivity, colour, Iron, dissolved oxygen, Calcium, Magnesium, alkalinity, total hardness, Manganese, Sulphate, Chloride, Phosphate, Sodium, Zinc, Copper, total dissolved solid, Nitrate, Nitrite, Ammonia, Ammonium and Potassium were determined following the procedures prescribed by American Public Health Association Standard Method. The bacteriological analysis was carried out using the standard microbiological standard for analysis of water for total and faecal coliform count. The mean temperature of the evaluated waters ranged from 23.03°C-29.3°C, mean pH ranged from 4.37-6.76, while turbidity had a mean range of 0.16 NTU-4.13 NTU. Conductivity ranged between 39.29 µs/cm - 120.7 µs/cm, dissolved oxygen with 13.30 mg/L - 4.19 mg/L, total dissolved solids ranged from 72.4 mg/L -23.5 mg/L, while the mean for iron concentration ranged from 0.12 mg/L - 0.99 mg/L. Similarly, the mean for total hardness was 34.2 mg/L - 17.1 mg/L and 7.93 mg/L - 6.71 mg/L for total alkalinity. Others includes Manganese (0.88 mg/L - 0.02 mg/L), Magnesium (16.5 mg/L - 9.9 mg/L), Calcium (9.77 mg/L - 7.20 mg/L, Nitrate (14.6 mg/L - 3.66 mg/L), Nitrite (0.076 mg/L -0.009 mg/L), Ammonia (0.89 mg/L - 0.25 mg/L), Ammonium (0.52 mg/L - 0.013 mg/L), Zinc (1.01 mg/L - 0.34 mg/L), Chloride (5.73 mg/L - 0.364 mg/L), Fluoride (0.76 mg/L - 0.277 mg/L), Copper (0.61 mg/L - 0.18 mg/L), Sodium (2.73 mg/L - 0.180 mg/L), potassium (5.73 mg/L - 2.0 mg/L), Sulphate (14.8 mg/L - 3.69 mg/L and Phosphate with 4.8 mg/L - 3.69 mg/L. The total coliform count for bottled water ranged between 2.00 cfu/100 mL – 19.00 cfu/100 mL, the total coliform range for sachet water were 6.00 cfu/100 mL and 15.00 cfu/100 mL and no faecal coliform was detected. Public water had no growth at all, the stream and borehole bacteriological analysis ranged from 27x101 cfu/ mL - 55x101 cfu/mL and 12 cfu/100 mL - 33 cfu/100 mL for total coliform respectively. Faecal coliform ranged from 15x101 cfu/ mL - 52x101 cfu/ mL for stream and 9.00 cfu100/ mL – 16.00 cfu/100 mL for borehole. A total of seven (7) different bacteria species were isolated from the sampled drinking water sources. These included Proteus spp., Streptococcus spp., Enterobacter spp., Pseudomonas spp., E.coli, Chromobacter spp., Salmonella spp. and Enterococcus spp. This study reveals a high level of poor quality sources of water in the metropolis and makes need for urgent health intervention.

scholarly journals Analysis of Microbial Contamination in Oreochromis Niloticus in Waduk Muara Nusa Dua

2020 ◽  
Vol 4 (1) ◽  
pp. 53-59
Author(s):  
Ni Kadek Peniasih ◽  
I Gede Suranaya Pandit ◽  
Dewa Gede Semara Edi
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Oreochromis Niloticus ◽  
Microbiological Quality ◽  
Water Source ◽  
Total Coliform ◽  
Microbiological Contamination ◽  
Reservoir Water ◽  
Objective Tests

This study aims to determine the microbiological contamination in the main water source of the Muara Nusa Dua Reservoir, the Tukad Badung River, and to determine the microbiological quality of fresh Oreochromis niloticus caught in the Muara Nusa Dua Reservoir in Denpasar. The study was conducted in May-August 2019 at the Denpasar Veterinary Laboratory. The method used is a descriptive method that aims to describe or describe the condition of the Tukad Badung watershed which empties into the Muara Nusa Dua Reservoir (Estuary Dam). Objective tests include the total bacterial test, the Coliform test, and the Escherichia coli contamination test. Water quality measurements are also carried out as supporting data from this study. The results of the study stated that there was microbiological contamination in the water source of the Muara Nusa Dua Reservoir, the Tukad Badung river, which also had an impact on the waters of the Muara Nusa Dua Reservoir. Microbiological contamination research results showed the microbiological quality of ten samples of Oreochromis niloticus in the form of the highest total bacteria was at 2.2x104, the highest total Coliform was 1.9x104 and the highest Escherichia coli contamination was at 93 APM / g with an average reservoir water quality Muara Nusa Dua in the form of temperature 27.3 °C, salinity 0.208 ‰, degree of acidity (pH) 6.5, dissolved oxygen (DO) 5,97 ppm. Organoleptic observations included the appearance, smell, and texture of Oreochromis niloticus with good results because when testing Oreochromis niloticus is still alive

scholarly journals Assessment of Drinking Waters Quality Collected from Boreholes in Afe Babalola University Ado-Ekiti (ABUAD)

2021 ◽  
Vol 384 ◽  
pp. 81-84
Author(s):  
Adeshina S. Adebanjo
Keyword(s):  
Drinking Water ◽  
Total Coliform ◽  
Poor Quality ◽  
Groundwater Exploration ◽  
World Health ◽  
Plate Count ◽  
Coliform Count ◽  
Quality Of Water ◽  
Two Samples

Abstract. The quality of drinking water is dependent of its source(s) and means of collection. The water available for drinking in the study area is accessible through groundwater exploration. This research was carried out to determine the quality of water from the selected boreholes within the university and checked by the World Health Organization (WHO) drinking water standards for the various parameters considered. Samples were gotten from seven (7) of the sited boreholes and various physico-chemical parameter tests such as turbidity test, conductivity test, pH test, copper test, manganese test, chloride test, sulphate test, chromium test, nitrate test and cadmium test and bacteriological tests as total plate count, total coliform count and faecal coliform count were carried out following due procedure, precautions and the results analyzed. The results show that most samples were satisfactory for the tests carried out while only two (samples from Behind Admin and Engineering) were unsatisfactory in turbidity while one sample (sample from Back of College 2) was unsatisfactory in the cadmium test. WQI showed that the water samples had excellent qualities except for that from Back of College 2 which had poor quality and is unsuitable consumption.

scholarly journals Microbiological Quality and Adulteration of Pasteurized and Raw Milk Marketed in Dharan, Nepal

2020 ◽  
pp. 37-44
Author(s):  
Dhiren Subba Limbu ◽  
Kamana Bantawa ◽  
Dil Kumar Limbu ◽  
Mandira Devkota ◽  
Monika Ghimire
Keyword(s):  
Microbiological Quality ◽  
Raw Milk ◽  
Total Coliform ◽  
Viable Count ◽  
Total Viable Count ◽  
Pasteurized Milk ◽  
Coliform Count ◽  
Standard Guideline ◽  
Routine Monitoring

 This study was aimed to evaluate the quality of raw and pasteurized milk marketed in Dharan. Milk may be contaminated with pathogenic microorganisms and a mixture of several adulterants and such milk pose a risk to consumers. The study was carried out from September 2019 to January 2020. Collected samples were tested for adulterants (starch, formalin, neutralizer and table sugar) as well as microbial quality (Total Coliform count, Total Viable Count, Thermoduric Count, Escherichia. coli and Staphylococcus aureus) as per standard guideline. The adulterants starch, formalin and neutralizer were not detected in both raw and pasteurized milk. However, table sugar was present in 45% (9 out of 20) raw milk and 90% (18 out of 20) pasteurized milk. The average Total Viable Count, Total Coliform Count and Thermoduric Count of raw milk were, 59×105 CFU/ml, 14×104 CFU/ml and 5×103 CFU/ml respectively. Similarly, the average Total Viable Count, Total Coliform Count and Thermoduric Count of pasteurized milk were found to be 15×104 CFU/ml, 14×103CFU/ml and 4×103 CFU/ml respectively. E. coli was detected in 30% pasteurized milk whereas S. aureus was isolated from only 20%. Likewise, E.coli and S. aureus were found in 55% and 45% of raw milk respectively. The results of the study indicated that routine monitoring of dairy industries and raw milk vendors, awareness campaign and good hygienic practice should be promoted to upgrade the quality of raw and pasteurized milk.

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