Novel Surface Coatings Modulating Eukaryotic Cell Adhesion and Preventing Implant Infection

2009 ◽  
Vol 32 (9) ◽  
pp. 655-662 ◽  
Author(s):  
JÜRGEN Groll ◽  
JÖRG Fiedler ◽  
Kristina Bruellhoff ◽  
Martin Moeller ◽  
Rolf E. Brenner
Keyword(s):  
Cell Adhesion ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Eukaryotic Cell ◽  
Organic Coating ◽  
Surface Coatings ◽  
Osteoblastic Cells ◽  
Orthopedic Implant ◽  
Small Peptide ◽  
Implant Infection

Poor osseointegration and bacterial infection are major causes of orthopedic implant failure. Both problems arise from passive unspecific protein coating that may not optimally support adhesion of osteoblastic cells and which enable bacterial adhesion that subsequently results in biofilm formation. This review addresses emerging concepts of preventing unspecific protein adsorption and biofilm formation by organic coating systems. We especially focus on recent concepts that additionally allow functionalization for preferential cell adhesion using cell adhesion mediating small peptide sequences that do not induce bacterial adherence. One promising approach that is presented and discussed within this context is the use of NCO-sP(EO-stat-PO).

Evaluation of the Anti-Bacterial Activity of a Novel Chelate-Setting Apatite Cement Containing Lactoferrin

2012 ◽  
Vol 529-530 ◽  
pp. 187-191 ◽  
Author(s):  
Hitomi Ohsugi ◽  
Yuri Habuto ◽  
Michiyo Honda ◽  
Mamoru Aizawa ◽  
Nobuyuki Kanzawa
Keyword(s):  
Escherichia Coli ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Bactericidal Effect ◽  
Bacterial Activity ◽  
Osteoblastic Cells ◽  
Inositol Hexaphosphate ◽  
Gram Negative ◽  
Apatite Cement ◽  
Surface Modified

We have developed a chelate-setting apatite cement. Synthesized hydroxyapatite (HAp) powders surface-modified with inositol hexaphosphate (IP6-HAp powder) were set by chelate-bonding with inositol hexaphosphate (IP6). Our aim is to fabricate IP6-HAp cement with anti-bacterial activity by adding lactoferrin (LF). It is known that LF has both anti-bacterial and osteoinductive activity. Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were used to examine the effect of LF on biofilm formation and localization of living and dead cells. In addition, the cell viability of MC3T3-E1 osteoblastic cells was determined. Our results show that the anti-bacterial activity of LF is not due to a bactericidal effect but to the inhibition of bacterial adhesion to surfaces. Furthermore, LF cement did not affect cell proliferation. Thus, LF cement is a candidate for bifunctional biomaterials having both anti-bacterial and osteo-conductive activity.

scholarly journals Enhancing Osseointegration and Mitigating Bacterial Biofilms on Medical-Grade Titanium with Chitosan-Conjugated Liquid-Infused Coatings

2021 ◽  
Author(s):  
Yuxi Zhang ◽  
Martin Villegas ◽  
Maryam Badv ◽  
Claudia Alonso-Cantu ◽  
David Wilson ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bone Cells ◽  
Bacterial Biofilms ◽  
Implant Infection ◽  
Device Failure ◽  
Tissue Integration ◽  
Biofilm Prevention ◽  
Medical Grade

Abstract Titanium alloys, in particular, medical-grade Ti-6Al-4V is heavily used in orthopaedic applications due to its high moduli, strength, and biocompatibility. Implant infection can result in biofilm formation and failure of prosthetics. The formation of a biofilm on implants protect bacteria from antibiotics and the immune response, resulting in the propagation of the infection and ultimately result in device failure. Recently, slippery liquid-infused surfaces (LIS) have been investigated for their stable liquid interface, which provide excellent repellent properties to suppress biofilm formation. One of the current limitations of LIS coatings lies in the indistinctive repellency of bone cells in orthopaedic applications, therefore causing poor integration between tissue and implant. Here, we report a chitosan impregnated LIS coating that facilitates cell adhesion and osseointegration while preventing biofilm formation. Our results indicate that chitosan-conjugated LIS increased cell adhesion of osteoblast-like SaOS-2 cells and significantly promoted proliferation compared to conventional titanium liquid-infused surfaces. Furthermore, the chitosan conjugated LIS significantly reduced biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) when compared to untreated and chitosan-coated titanium. Our engineered coating can be easily modified with other biopolymers or capture molecules to be applied to other biomaterials where both tissue integration and biofilm prevention is needed.

scholarly journals Application of Antimicrobial Peptides on Biomedical Implants: Three Ways to Pursue Peptide Coatings

2021 ◽  
Vol 22 (24) ◽  
pp. 13212
Author(s):  
Marco G. Drexelius ◽  
Ines Neundorf
Keyword(s):  
Postoperative Complications ◽  
Antimicrobial Peptides ◽  
Medical Devices ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Surface Coatings ◽  
Implant Failure ◽  
Recent Developments ◽  
Peptide Coatings ◽  
Antibiotic Agents

Biofilm formation and inflammations are number one reasons of implant failure and cause a severe number of postoperative complications every year. To functionalize implant surfaces with antibiotic agents provides perspectives to minimize and/or prevent bacterial adhesion and proliferation. In recent years, antimicrobial peptides (AMP) have been evolved as promising alternatives to commonly used antibiotics, and have been seen as potent candidates for antimicrobial surface coatings. This review aims to summarize recent developments in this field and to highlight examples of the most common techniques used for preparing such AMP-based medical devices. We will report on three different ways to pursue peptide coatings, using either binding sequences (primary approach), linker layers (secondary approach), or loading in matrixes which offer a defined release (tertiary approach). All of them will be discussed in the light of current research in this area.

scholarly journals Bond Strengthening in Oral Bacterial Adhesion to Salivary Conditioning Films

2008 ◽  
Vol 74 (17) ◽  
pp. 5511-5515 ◽  
Author(s):  
Henny C. van der Mei ◽  
Minie Rustema-Abbing ◽  
Joop de Vries ◽  
Henk J. Busscher
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Specific Interactions ◽  
Adhesion Forces ◽  
Bacterial Strains ◽  
Bacterial Interactions ◽  
Initial Adhesion ◽  
Conditioning Film ◽  
Interacting Surfaces ◽  
Conditioning Films

ABSTRACT Transition from reversible to irreversible bacterial adhesion is a highly relevant but poorly understood step in initial biofilm formation. We hypothesize that in oral biofilm formation, irreversible adhesion is caused by bond strengthening due to specific bacterial interactions with salivary conditioning films. Here, we compared the initial adhesion of six oral bacterial strains to salivary conditioning films with their adhesion to a bovine serum albumin (BSA) coating and related their adhesion to the strengthening of the binding forces measured with bacteria-coated atomic force microscopy cantilevers. All strains adhered in higher numbers to salivary conditioning films than to BSA coatings, and specific bacterial interactions with salivary conditioning films were accompanied by stronger initial adhesion forces. Bond strengthening occurred on a time scale of several tens of seconds and was slower for actinomyces than for streptococci. Nonspecific interactions between bacteria and BSA coatings strengthened twofold faster than their specific interactions with salivary conditioning films, likely because specific interactions require a closer approach of interacting surfaces with the removal of interfacial water and a more extensive rearrangement of surface structures. After bond strengthening, bacterial adhesion forces with a salivary conditioning film remained stronger than those with BSA coatings.

scholarly journals Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation In Vitro and In Vivo

2007 ◽  
Vol 6 (6) ◽  
pp. 931-939 ◽  
Author(s):  
Fang Li ◽  
Michael J. Svarovsky ◽  
Amy J. Karlsson ◽  
Joel P. Wagner ◽  
Karen Marchillo ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Cell Adhesion ◽  
Biofilm Formation ◽  
Fungal Infections ◽  
Gene Dosage ◽  
Venous Catheter ◽  
Flow Chamber ◽  
Dependent Manner

ABSTRACT Candida albicans is the leading cause of systemic fungal infections in immunocompromised humans. The ability to form biofilms on surfaces in the host or on implanted medical devices enhances C. albicans virulence, leading to antimicrobial resistance and providing a reservoir for infection. Biofilm formation is a complex multicellular process consisting of cell adhesion, cell growth, morphogenic switching between yeast form and filamentous states, and quorum sensing. Here we describe the role of the C. albicans EAP1 gene, which encodes a glycosylphosphatidylinositol-anchored, glucan-cross-linked cell wall protein, in adhesion and biofilm formation in vitro and in vivo. Deleting EAP1 reduced cell adhesion to polystyrene and epithelial cells in a gene dosage-dependent manner. Furthermore, EAP1 expression was required for C. albicans biofilm formation in an in vitro parallel plate flow chamber model and in an in vivo rat central venous catheter model. EAP1 expression was upregulated in biofilm-associated cells in vitro and in vivo. Our results illustrate an association between Eap1p-mediated adhesion and biofilm formation in vitro and in vivo.

scholarly journals A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2771
Author(s):  
Tobias Wieland ◽  
Julia Assmann ◽  
Astrid Bethe ◽  
Christian Fidelak ◽  
Helena Gmoser ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Real Time ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Inhibitory Effect ◽  
Thermal Sensor ◽  
Bacterial Cells ◽  
Static Conditions

The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated Escherichia (E.) coli and Staphylococcus (S.) aureus. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs.

Bacterial adhesion to collagens: implications for biofilm formation and disease progression in the oral cavity

2021 ◽  
pp. 1-13
Author(s):  
Simón Álvarez ◽  
Camila Leiva-Sabadini ◽  
Christina M. A. P. Schuh ◽  
Sebastian Aguayo
Keyword(s):  
Oral Cavity ◽  
Disease Progression ◽  
Bacterial Adhesion ◽  
Biofilm Formation

Monolithic Ceramics: Effect of Finishing Techniques on Surface Properties, Bacterial Adhesion and Cell Viability

2018 ◽  
Vol 43 (3) ◽  
pp. 315-325 ◽  
Author(s):  
AMO Dal Piva ◽  
LPC Contreras ◽  
FC Ribeiro ◽  
LC Anami ◽  
SEA Camargo ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Nonparametric Tests ◽  
Lithium Silicate ◽  
Gingival Fibroblasts ◽  
Sem Images ◽  
High Profile ◽  
Colony Forming Units ◽  
Monolithic Ceramics

SUMMARY Introduction: This study evaluated the morphology, biofilm formation, and viability of human gingival fibroblasts in contact with two monolithic ceramics after two different finishing techniques: polishing or glazing. For this, 92 blocks (4.5 × 4.5 × 1.5 mm) of each ceramic were made using high translucency zirconia partially stabilized by yttrium (YZHT) and lithium silicate reinforced by zirconium (ZLS). Methods and Materials: Blocks were sintered and then divided into glazing (g) or polishing (p) surface finish. Surface roughness (Ra and RSm) was evaluated through a contact rugosimeter and profilometry. Specimens were contaminated for heterotypic biofilm formation with Streptococcus mutans, Streptococcus sanguinis and Candida albicans for 16 hours. Biofilm was quantified by counting the colony forming units (CFU/mL) and analyzed by scanning electron microscopy (SEM). Fibroblast viability was evaluated by MTT assay. Surface free energy (SFE) was also determined. Roughness data were evaluated using nonparametric tests, while SFE, MTT and CFU results were evaluated by analysis of variance and Tukey test, and MTT data were also submitted to t-test (all, α=0.05). Results: Results showed that polished samples presented a lower high profile mean (p<0.001); however, YZHTg presented less space between defects (p=0.0002). SFE showed that YZHT presented higher SFE than ZLS. Profilometry evidenced more homogeneity on polished surfaces. The interaction of finishing technique and microorganisms influenced the CFU (p=0.00). MTT assay demonstrated initial severe cytotoxic behavior for polished surfaces. SEM images showed homogeneous surfaces, except for glazed YZHT. Conclusion: Glazed surfaces have a greater roughness and tend to accumulate more biofilm. Polished surfaces have higher SFE; however, they are temporarily cytotoxic.

scholarly journals Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

2016 ◽  
Vol 59 ◽  
pp. 524-532 ◽  
Author(s):  
Maria Godoy-Gallardo ◽  
Jordi Guillem-Marti ◽  
Pablo Sevilla ◽  
José M. Manero ◽  
Francisco J. Gil ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Osteoblast Cell ◽  
Titanium Surfaces

scholarly journals Evolutionary Perspectives on the Moonlighting Functions of Bacterial Factors That Support Actin-Based Motility

mBio ◽  
2019 ◽  
Vol 10 (4) ◽  
Author(s):  
Volkan K. Köseoğlu ◽  
Hervé Agaisse
Keyword(s):  
Cell Adhesion ◽  
Host Cell ◽  
Biofilm Formation ◽  
Actin Polymerization ◽  
Current Knowledge ◽  
Bacterial Pathogens ◽  
Actin Nucleation ◽  
Bacterial Aggregation ◽  
Moonlighting Functions ◽  
Evolutionary Perspectives

ABSTRACT Various bacterial pathogens display an intracellular lifestyle and spread from cell to cell through actin-based motility (ABM). ABM requires actin polymerization at the bacterial pole and is mediated by the expression of bacterial factors that hijack the host cell actin nucleation machinery or exhibit intrinsic actin nucleation properties. It is increasingly recognized that bacterial ABM factors, in addition to having a crucial task during the intracellular phase of infection, display “moonlighting” adhesin functions, such as bacterial aggregation, biofilm formation, and host cell adhesion/invasion. Here, we review our current knowledge of ABM factors and their additional functions, and we propose that intracellular ABM functions have evolved from ancestral, extracellular adhesin functions.

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