Sodium metabisulfite induces lipid peroxidation and apoptosis in rat gastric tissue

Sodium metabisulfite (NaMBS) has been used as an antioxidant and antimicrobial agent in a variety of drugs and foods. The aim of our study was to evaluate the effect of subchronic ingestion of NaMBS on lipid peroxidation, protein and enzymatic antioxidants in the gastric tissue and splenic Wistar rat. The rats were divided into four groups (n = 06 rats/group) as follows; Control (C), rats treated with 0.25% NaMBS (S0.25%), rats treated with 1% NaMBS (S1%) and rats treated with 4% NaMBS (S4%). NaMBS was administered to animals via drinking water. At the end of the experimental period, Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxi-dase (GPx), catalase (CAT) and thiols were determined. Redox status assess-ment revealed that 1% and 4% NaMBS doses induced gastric and spleen lipid peroxidation in animals compared to the control group. On the other hand, activities of SOD, GPx, CAT and thiols were reduced in the S1% and S4% groups in the stomach and spleen compared to the controls. Slight changes were detected in the GPx and CAT in the stomach of S0.25% group compared to the control group. In conclusion, the subchronic consumption of NaMBS has an adverse effect on the spleen and stomach by inducing oxidative damage leading to an increase in lipid peroxidation, protein oxidation and alteration of enzyme activity antioxidant.

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Role of Lipid Composition and Lipid Peroxidation in the Sensitivity of Fungal Plant Pathogens to Aluminum Chloride and Sodium Metabisulfite

Applied and Environmental Microbiology ◽

10.1128/aem.02849-06 ◽

2007 ◽

Vol 73 (9) ◽

pp. 2820-2824 ◽

Cited By ~ 18

Author(s):

Tyler J. Avis ◽

Mélanie Michaud ◽

Russell J. Tweddell

Keyword(s):

Lipid Peroxidation ◽

Fatty Acid ◽

Aluminum Chloride ◽

Plant Pathogens ◽

Membrane Lipids ◽

Membrane Integrity ◽

Sodium Metabisulfite ◽

Fatty Acid Unsaturation ◽

Fungal Plant Pathogens

ABSTRACT Aluminum chloride and sodium metabisulfite have shown high efficacy at low doses in controlling postharvest pathogens on potato tubers. Direct effects of these two salts included the loss of cell membrane integrity in exposed pathogens. In this work, four fungal potato pathogens were studied in order to elucidate the role of membrane lipids and lipid peroxidation in the relative sensitivity of microorganisms exposed to these salts. Inhibition of mycelial growth in these fungi varied considerably and revealed sensitivity groups within the tested fungi. Analysis of fatty acids in these fungi demonstrated that sensitivity was related to high intrinsic fatty acid unsaturation. When exposed to the antifungal salts, sensitive fungi demonstrated a loss of fatty acid unsaturation, which was accompanied by an elevation in malondialdehyde content (a biochemical marker of lipid peroxidation). Our data suggest that aluminum chloride and sodium metabisulfite could induce lipid peroxidation in sensitive fungi, which may promote the ensuing loss of integrity in the plasma membrane. This direct effect on fungal membranes may contribute, at least in part, to the observed antimicrobial effects of these two salts.

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Effect of Sodium Metabisulfite on Oxidative Stress and Lipid Peroxidation Biomarkers

Current Nutrition & Food Science ◽

10.2174/1573401314666181024130333 ◽

2020 ◽

Vol 16 (1) ◽

pp. 114-117

Author(s):

Shahnaz Shekarforoush ◽

Parisa Ebrahimi ◽

Akbar Afkhami Fathabad ◽

Elaheh Farzanfar

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Status ◽

Treated Group ◽

Control Group ◽

Antioxidant Enzyme Activities ◽

Sodium Metabisulfite ◽

Male Wistar Rats ◽

Pharmaceutical Agents ◽

Cellular Components ◽

Dose Dependent

Background: Sulfites are widely used as preservatives in the foods and pharmaceutical agents. It has been demonstrated that sulfites can react with a variety of cellular components and cause toxicity. Objective: The present study was designed to investigate the effects of ingested sodium metabisulfite (SMB) on serum antioxidant status in rats. Methods: Thirty-two male Wistar rats were randomly divided into control and treated groups. Treated groups received 10, 100, and 260 mg/kg body weight of SMB for 28 days. After 28 days, serum was assayed for measuring superoxide dismtase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) activities, glutathion (GSH) level and lipid peroxidation. Results: The results showed that the activities of GPx, GR, CAT and GSH levels were significantly decreased in 100 and 260 mg/kg SMB treated rats, while malondialdehyde (MDA) level was significantly increased in 260 mg/kg treated group when compared with the control group. Conclusion: It is concluded that SMB administration as dose-dependent is associated with decreased serum antioxidant enzyme activities and increased lipid peroxidation.

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Prooxidant activity of norbixin in model of acute gastric ulcer induced by ethanol in rats

Human & Experimental Toxicology ◽

10.1177/0960327115604199 ◽

2015 ◽

Vol 35 (7) ◽

pp. 737-746 ◽

Cited By ~ 2

Author(s):

BT Rovani ◽

RB de Freitas ◽

PR Augusti ◽

IC Araldi ◽

S Somacal ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Gastric Ulcer ◽

Food Industry ◽

Wistar Rats ◽

Gastric Tissue ◽

Prooxidant Activity ◽

Prooxidant Effect ◽

Male Wistar Rats ◽

And Oxidative Stress

Free radicals and oxidative stress play a central role in gastric injuries caused by ethanol (EtOH). Antioxidant strategies to counteract EtOH toxicity are highly desirable. Norbixin (NBIX) is a carotenoid with antioxidant potential largely used in the food industry. This study evaluated the NBIX effects in a model of gastric ulcer induced by EtOH in rats. Male Wistar rats received NBIX doses of 0, 10, and 25 mg/kg by gavage 1 h after EtOH administration (0 or 75% solution, 1 mL/200 g of animal). The animals were euthanized 1 h after the NBIX administration, and their stomachs were removed for macroscopic and histopathological analyses, quantification of nonprotein sulfhydryl (NPSH) groups, lipid peroxidation (LPO) levels, and catalase (CAT) activity determination. NBIX increased LPO in gastric mucosa and caused CAT inhibition and NPSH depletion in EtOH-treated animals. Results showed that NBIX did not protect gastric tissue against EtOH damage, and this could be associated to a prooxidant effect.

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Gastro-protective actions of Aloe barbadensis Miller mitigate ethanol-induced gastric injury in rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i12.24 ◽

2021 ◽

Vol 19 (12) ◽

pp. 2645-2650

Author(s):

Kai Zhu ◽

Xia Yang ◽

Chun Yang ◽

Xiaoxue Ye ◽

Hongxing Zhang

Keyword(s):

Lipid Peroxidation ◽

Gastric Ulcer ◽

Gastric Mucosa ◽

Gastric Juice ◽

Histological Examination ◽

Leaf Extract ◽

Gastric Injury ◽

Control Group ◽

Gastric Tissue ◽

Aloe Barbadensis

Purpose: To investigate the gastroprotective effect of leaf extract of Aloe barbadensis on ethanolinduced gastric ulcer in rats.Methods: Healthy male Wistar rats (n = 30) weighing 180 - 220 g (mean weight = 200 ± 20 g) were randomly assigned to 6 groups (5 rats/group): control group, gastric ulcer group, two Aloe barbadensis extract groups (250 and 500 mg/kg), cimetidine group and indomethacin group. Gastric ulcer was induced via oral injection of absolute ethanol at a dose of 1 mL/kg after a 24-h fast. Gross evaluation, determination of gastric juice acidity and histological examination of gastric tissue were carried out.Results: Treatment of gastric ulcer rats with Aloe barbadensis extract significantly reduced ulcerated area (UA), ulcer index (UI), and acidity of gastric juice (p < 0.05). Injection of 1 % carrageenan into rat hind paw led to a time-dependent increase in paw volume which peaked 3 h after injection. However, the Aloe barbadensis extract significantly and dose-dependently reduced the volume of inflamed paw, and inhibited edema formation (p < 0.05). It also markedly reduced or completely eliminated edema and leucocyte infiltration. Moreover, treatment of gastric ulcer rats with Aloe barbadensis leaf extract led to significant and dose-dependent reduction in gastric tissue MDA level (p < 0.05). Histological examination of the gastric wall showed that control rats had severe injury in gastric mucosa,accompanied by edema and leucocytes infiltration, relative to rats pretreated with extract which showed marked gastric protection and inhibition of edema and leucocytes infiltration. Moreover, the extract treatment protected the gastric surface against ulceration as indicated by reduced lesions in the treated rat model.Conclusion: These results show that Aloe barbadensis mitigates ethanol-induced gastric injury in rats via inhibition of lipid peroxidation and inflammation. Thus, the extract has potentials for development into a therapeutic agent for the management of gastric ulcer. Keywords: Aloe barbadensis, Gastric ulcer, Gastric mucosa, Inflammation, Lipid peroxidation

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The Effects of Garlic Oil and Tartaric Acid on the Quality of Shrimp Stored at 4°C

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.448 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Wan Norhana Md Noordin ◽

Nannthini Shunmugam ◽

Frederick Adzitey ◽

Nurul Huda

Keyword(s):

Lipid Peroxidation ◽

Tartaric Acid ◽

Foodborne Pathogens ◽

Storage Period ◽

Thiobarbituric Acid ◽

Plate Count ◽

Sodium Metabisulfite ◽

Garlic Oil ◽

Serovar Typhimurium ◽

Tbars Values

The objectives of this study were to determine the effects of garlic oil (GO) and tartaric acid (TA) on microbiological, pH, and Thiobarbituric Acid Reactive Substances (TBARS) (lipid peroxidation) of shrimp stored at 4°C. Shrimp of 2 kg were dipped in GO and TA solutions at 1:2 shrimp/treatment solutions (w/w) for 30 mins under 25ºC. Sodium metabisulfite (MBS) and sterile distilled water (dH20) were used as positive and negative controls, respectively. Shrimp were drip-dried for 5 mins, packaged, and stored in a chiller (4°C) for 10 days. The shrimp were analysed on days 0, 3, 5, 7, and 10. Total aerobic plate count, psychotropic bacteria count, Listeria monocytogenes, Salmonella enterica serovar Typhimurium (S. Typhimurium) and Vibrio parahaemolyticus counts ranged from 3.52-8.73 log CFU/g, <3.30-5.16 log CFU/g, 3.48-7.60 log CFU/g, 3.42-6.34 log CFU/g and 3.48-5.55 log CFU/g, respectively. The pH of shrimp ranged from 6.64 to 8.03. The pH of shrimp dipped in MBS (7.70) and TA+GO (7.70) was lowest at the end of storage period. TBARS values ranged from 0.70-2.66 and TBARS values for shrimp treated with MBS (1.83) were lowest at day 10. In general, microbiological counts, pH and TBARS values of sample treated with TA+GO increased with storage time, however comparable to MBS. Treatment of shrimp with GO and TA could inhibit the growth of foodborne pathogens. The pH of the test and control shrimp were similar on day 10. Lipid peroxidation was lowest for TA and MBS treated shrimp by day 10.

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Effect of Sodium Metabisulfite on Rat Ovary and Lipid Peroxidation

Iranian Jornal of Toxicology ◽

10.32598/ijt.10.2.316.1 ◽

2016 ◽

Vol 10 (2) ◽

pp. 23-28

Author(s):

Nahid Rezaee ◽

◽

Zahra Nematollahi ◽

Shahnaz Shekarforous ◽

Ebrahim Hoseini ◽

...

Keyword(s):

Lipid Peroxidation ◽

Structural Changes ◽

Ovarian Tissue ◽

Critical Role ◽

Food Additives ◽

Dietary Factors ◽

Female Rats ◽

Control Group ◽

Sodium Metabisulfite ◽

Ovarian Volume

Background: Many health problems are related to lifestyle and dietary factors. Since ancient times, food additives such as sulfites have been used to preserve foods. Diverse effects of sulfites on multiple organs have been reported but its effect on female reproductive organ has not been fully elucidated. The aim of this study was to investigate the effects of sodium metabisulfite (SMB) on ovarian tissue in adult rats. Methods: Four groups of female rats (n=32) were used. The experimental rats received 10, 100 and 260 mg/kg SMB for 28 days (S10, S100 and S260 groups, respectively). The control rats received distilled water for the same period. The ovarian volume, weight and the number of different types of follicles were estimated by stereological methods. Lipid peroxidation is assessed indirectly by the measurement of malondialdehyde (MDA), using the thiobarbituric acid (TBA) method. Results: The results showed a significant decrease in the ovarian volume, the number of primordial, primary, secondary, grafian follicles and corpus luteum in the SMB-treated animals compared with the control group (P < 0.05). In comparison to the control group, the number of atretic follicles increased in the SMB-treated rats. MDA was significantly increased in S260 group compared to the control group. Conclusion: The present data confirm sulfite-induced structural changes in the ovary. Increased level of MDA because of SMB ingestion suggests that free radicals may have a critical role in these changes.

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Sulfite Supported Lipid Peroxidation in Propofol Emulsions

Anesthesiology ◽

10.1097/00000542-200211000-00021 ◽

2002 ◽

Vol 97 (5) ◽

pp. 1162-1167 ◽

Cited By ~ 19

Author(s):

Max T. Baker ◽

Deborah J. Dehring ◽

Marc S. Gregerson

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Soybean Oil ◽

Thiobarbituric Acid ◽

Glass Electrode ◽

Peroxidation Of Lipids ◽

Sodium Metabisulfite ◽

Oil Emulsion ◽

Nitrobenzoic Acid ◽

Oil Emulsions

Background Sodium metabisulfite is added to a commercial propofol emulsion as an antimicrobial agent. The sulfite ion (SO3(-2)) is capable of undergoing a number of reactions, including autooxidation and the promotion of lipid peroxidation. This study evaluated sulfite reactivity in propofol emulsions by determining thiobarbituric acid reacting substances (TBARS), sulfite depletion, and emulsion pH in emulsions containing sulfite or EDTA. Methods Commercial EDTA and sulfite propofol emulsions were compared, and 10% soybean oil emulsion containing various additives were evaluated for TBARS, sulfite, and pH. TBARS were analyzed with a modified thiobarbituric acid method. Sulfite was analyzed by the reaction of sulfite with 5,5'-dithiobis(2-nitrobenzoic acid). pH was measured by glass electrode methodology. Results Thiobarbituric acid reacting substances were detectable in commercial sulfite propofol emulsions in concentrations ranging from 0.02 to 0.22 microg/ml based on malondialdehyde. No TBARS were detected in EDTA propofol emulsions. Incubation (37 degrees C, up to 6 h) of sulfite propofol emulsions in air resulted in further increases in TBARS (35-160%). No increases occurred in incubated EDTA propofol emulsions. Metabisulfite (0.25 mg/ml) alone added to 10% soybean oil resulted in large increases in TBARS that were inhibited in part by propofol (10 mg/ml) and completely by ascorbic acid (0.05 mg/ml). Soybean oil emulsion pH declined rapidly on the addition of metabisulfite (0.25 mg/ml). The addition of propofol (10 mg/ml) partially inhibited the decline in pH and ascorbic acid (0.05 mg/ml) completely inhibited it. Conclusion These results show that sulfite supports the peroxidation of lipids in soybean oil emulsions and propofol functions to partially inhibit these processes.

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The Effects of Garlic Oil and Tartaric Acid on the Quality of Shrimp Stored at 4°C

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v0i0.448 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Wan Norhana Md Noordin ◽

Nannthini Shunmugam ◽

Frederick Adzitey ◽

Nurul Huda

Keyword(s):

Lipid Peroxidation ◽

Tartaric Acid ◽

Foodborne Pathogens ◽

Storage Period ◽

Thiobarbituric Acid ◽

Plate Count ◽

Sodium Metabisulfite ◽

Garlic Oil ◽

Serovar Typhimurium ◽

Tbars Values

The objectives of this study were to determine the effects of garlic oil (GO) and tartaric acid (TA) on microbiological, pH, and Thiobarbituric Acid Reactive Substances (TBARS) (lipid peroxidation) of shrimp stored at 4°C. Shrimp of 2 kg were dipped in GO and TA solutions at 1:2 shrimp/treatment solutions (w/w) for 30 mins under 25ºC. Sodium metabisulfite (MBS) and sterile distilled water (dH20) were used as positive and negative controls, respectively. Shrimp were drip-dried for 5 mins, packaged, and stored in a chiller (4°C) for 10 days. The shrimp were analysed on days 0, 3, 5, 7, and 10. Total aerobic plate count, psychotropic bacteria count, Listeria monocytogenes, Salmonella enterica serovar Typhimurium (S. Typhimurium) and Vibrio parahaemolyticus counts ranged from 3.52-8.73 log CFU/g, <3.30-5.16 log CFU/g, 3.48-7.60 log CFU/g, 3.42-6.34 log CFU/g and 3.48-5.55 log CFU/g, respectively. The pH of shrimp ranged from 6.64 to 8.03. The pH of shrimp dipped in MBS (7.70) and TA+GO (7.70) was lowest at the end of storage period. TBARS values ranged from 0.70-2.66 and TBARS values for shrimp treated with MBS (1.83) were lowest at day 10. In general, microbiological counts, pH and TBARS values of sample treated with TA+GO increased with storage time, however comparable to MBS. Treatment of shrimp with GO and TA could inhibit the growth of foodborne pathogens. The pH of the test and control shrimp were similar on day 10. Lipid peroxidation was lowest for TA and MBS treated shrimp by day 10.

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