scholarly journals Gastro-protective actions of Aloe barbadensis Miller mitigate ethanol-induced gastric injury in rats

2021 ◽  
Vol 19 (12) ◽  
pp. 2645-2650
Author(s):  
Kai Zhu ◽  
Xia Yang ◽  
Chun Yang ◽  
Xiaoxue Ye ◽  
Hongxing Zhang
Keyword(s):  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Gastric Juice ◽  
Histological Examination ◽  
Leaf Extract ◽  
Gastric Injury ◽  
Control Group ◽  
Gastric Tissue ◽  
Aloe Barbadensis

Purpose: To investigate the gastroprotective effect of leaf extract of Aloe barbadensis on ethanolinduced gastric ulcer in rats.Methods: Healthy male Wistar rats (n = 30) weighing 180 - 220 g (mean weight = 200 ± 20 g) were randomly assigned to 6 groups (5 rats/group): control group, gastric ulcer group, two Aloe barbadensis extract groups (250 and 500 mg/kg), cimetidine group and indomethacin group. Gastric ulcer was induced via oral injection of absolute ethanol at a dose of 1 mL/kg after a 24-h fast. Gross evaluation, determination of gastric juice acidity and histological examination of gastric tissue were carried out.Results: Treatment of gastric ulcer rats with Aloe barbadensis extract significantly reduced ulcerated area (UA), ulcer index (UI), and acidity of gastric juice (p < 0.05). Injection of 1 % carrageenan into rat hind paw led to a time-dependent increase in paw volume which peaked 3 h after injection. However, the Aloe barbadensis extract significantly and dose-dependently reduced the volume of inflamed paw, and inhibited edema formation (p < 0.05). It also markedly reduced or completely eliminated edema and leucocyte infiltration. Moreover, treatment of gastric ulcer rats with Aloe barbadensis leaf extract led to significant and dose-dependent reduction in gastric tissue MDA level (p < 0.05). Histological examination of the gastric wall showed that control rats had severe injury in gastric mucosa,accompanied by edema and leucocytes infiltration, relative to rats pretreated with extract which showed marked gastric protection and inhibition of edema and leucocytes infiltration. Moreover, the extract treatment protected the gastric surface against ulceration as indicated by reduced lesions in the treated rat model.Conclusion: These results show that Aloe barbadensis mitigates ethanol-induced gastric injury in rats via inhibition of lipid  peroxidation and inflammation. Thus, the extract has potentials for development into a therapeutic agent for the management of  gastric ulcer. Keywords: Aloe barbadensis, Gastric ulcer, Gastric mucosa, Inflammation, Lipid peroxidation

scholarly journals The haemato-protective effect of Piliostigma thonningii leaf extract on indomethacin-induced gastric mucosa ulcer in rats

2018 ◽  
Vol 7 (8) ◽  
pp. 1454
Author(s):  
A. A. Asuk ◽  
K. Dasofunjo ◽  
M. N. Ugwu ◽  
F. O. Adie
Keyword(s):  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Leaf Extract ◽  
Promising Result ◽  
Gastrointestinal Disorder ◽  
Male Rats ◽  
Control Group ◽  
Hematological Indices ◽  
Group I ◽  
Piliostigma Thonningii

Background: Gastric ulcer is a common gastrointestinal disorder with global consequence, which is aggravated by an imbalance between the aggressive factors and factors that maintain the mucosal integrity. The role of Piliostigma thonningii leaf extract on hematological indices of indomethacin-induced gastric mucosa lesions in Wistar rats was examined.Methods: Thirty-six male rats were divided into six groups of 6 rats each. Group I, the normal control, II gastric ulcerated + cimetidine (standard control), III extract only (100mg/kg bwt), while IV, gastric ulcerated control, V gastric ulcerated + extract (100mg/kg bwt) and VI gastric ulcerated + extract (200mg/kg bwt). After 12 days of administration, gastric ulcer was then induced by oral administration of 40mg/kg bwt indomethacin to rats in groups II, IV, V and VI. The rats were sacrificed 12 hours after indomethacin treatment and blood collected for hematological assay.Results: The RBC count and Hb pattern were similar. There was a significant (P<0.05) decrease in RBC and Hb of the ulcer control group which was normalized on administration of 200mg/kg bwt of P. thonningii extract. The MCV of the ulcer lesion was significantly (P<0.05) reversed to normal by the extract.Conclusions: The P. thonningii leaf extract showed promising result by normalizing decreased levels in RBC and Hb caused by ulcer. Except for platelet counts, the WBC count and differential WBC counts were quite positive. It was able to reverse macrocytosis caused by ulcer lesions to normal, hence exhibiting a hemato-protective nature.

scholarly journals Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Kai Zhu ◽  
Peng Peng ◽  
Ning Wu ◽  
Xianrong Zhou ◽  
Jianfei Mu ◽  
...  
Keyword(s):  
Gastric Juice ◽  
Tea Polyphenols ◽  
Gastric Injury ◽  
Mouse Serum ◽  
Gastric Tissue ◽  
Preventive Effect ◽  
High Concentration ◽  
Sod Activity ◽  
Bioactive Substances ◽  
Gastric Mucosal Lesions

Liupao tea is a traditional Chinese tea drink. The preventive effect of crude polyphenols in Liupao tea on HCl/ethanol-induced gastric injury was investigated in this study. After a model of gastric injury in mice was established, mouse serum and tissues were analyzed by biochemical and molecular biological methods. The results showed that Liupao tea polyphenols (LTPs) could effectively reduce the area of gastric mucosal lesions, decrease the volume of gastric juice, and increase the pH of gastric juice in mice with gastric injury. Observations of the pathology revealed that LTPs could alleviate cell necrosis and gastric mucosal injury in mice with gastric injury. The SOD activity and GSH level were decreased in mice after gastric injury, while the level of MDA was increased. LTPs could inhibit the changes caused by gastric injury and make the SOD activity, GSH, and MDA levels close to the normal levels. In addition, LTPs could upregulate the mRNA expression of Cu/Zn-SOD, Mn-SOD, CAT, nNOS, and eNOS and downregulate the expression of iNOS in the gastric tissue of mice with gastric injury. Therefore, LTPs can effectively prevent HCl/ethanol-induced gastric injury. HPLC analysis showed that LTP contains six bioactive substances of gallic acid, catechin, caffeine, epicatechin, epigallocatechin gallate, and epicatechin gallate, so the effect of LTP might mainly come from these six components. The effect of a high concentration of LTP is similar to that of ranitidine. LTPs represent a kind of active substance with a protective effect on gastric tissue.

scholarly journals Anti-inflammatory activity of acacia catechu-bark aqueous solution in aspirin induced gastric ulcer in rodents

2021 ◽  
Vol 8 (12) ◽  
pp. 5649
Author(s):  
Uzma Waseem ◽  
Syeda Rizwana Jafri ◽  
Sarah Khalid ◽  
Fauzia Qureshi ◽  
Nadia Majeed ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Inflammatory Cells ◽  
Protective Role ◽  
Gastric Injury ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
Anti Inflammatory ◽  
Group B ◽  
Acacia Catechu

Background: Aspirin is amongst the most widely used drugs and has many adverse effects on gastric mucosa. Anti-inflammatory properties of Acacia catechu have been established already. Objective was to evaluate the histopathological changes induced by aspirin in the stomach of albino rats and to assess the protective effect of different doses of Acacia catechu.Methods: Experimental study Postgraduate Medical Institute, Lahore for 21 days. Forty-eight adult albino rats, both males and female, were divided into four groups A, B, C and D randomly; each comprising of 12 rats. Group A was control, group B was given aspirin 100 mg/kg and group C and D were given aspirin 100 mg/kg along with Acacia catechu 250 mg/kg and 500 mg/kg respectively by oral route. The rats from individual group were sacrificed on 3rd day, 7th day and 14th day and stomachs were examined under light microscope to observe the inflammatory cells infiltration.Results: Gross and microscopic findings on days 3, 7 and 14 were similar. Control groups A1, A2 and A3 showed normal healthy gastric mucosa and the least number of inflammatory cells. In group B, aspirin produced ulcerations and linear breaks; with highest inflammatory infiltrates. On microscopic examination, numerous inflammatory cells were noted. Group C and D rats had minimum ulcer index and fewer inflammatory cells.Conclusions: Acacia catechu has protective role against gastric injury by inhibiting inflammation. 

Effect of sodium metabisulfite on lipid peroxidation and enzyme activities in adult rat stomach and spleen

2017 ◽  
Vol 7 (1) ◽  
pp. 01-08
Author(s):  
Elkadi Fatima Zohra ◽  
Boufadi Mokhtaria Yasmina ◽  
Meziani Samira ◽  
Sarsar Fatima Zohra ◽  
Djebara Soraya ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Experimental Period ◽  
Wistar Rat ◽  
Redox Status ◽  
Control Group ◽  
Sodium Metabisulfite ◽  
Enzymatic Antioxidants ◽  
Gastric Tissue ◽  
Rat Stomach ◽  
Adult Rat

Sodium metabisulfite (NaMBS) has been used as an antioxidant and antimicrobial agent in a variety of drugs and foods. The aim of our study was to evaluate the effect of subchronic ingestion of NaMBS on lipid peroxidation, protein and enzymatic antioxidants in the gastric tissue and splenic Wistar rat. The rats were divided into four groups (n = 06 rats/group) as follows; Control (C), rats treated with 0.25% NaMBS (S0.25%), rats treated with 1% NaMBS (S1%) and rats treated with 4% NaMBS (S4%). NaMBS was administered to animals via drinking water. At the end of the experimental period, Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxi-dase (GPx), catalase (CAT) and thiols were determined. Redox status assess-ment revealed that 1% and 4% NaMBS doses induced gastric and spleen lipid peroxidation in animals compared to the control group. On the other hand, activities of SOD, GPx, CAT and thiols were reduced in the S1% and S4% groups in the stomach and spleen compared to the controls. Slight changes were detected in the GPx and CAT in the stomach of S0.25% group compared to the control group. In conclusion, the subchronic consumption of NaMBS has an adverse effect on the spleen and stomach by inducing oxidative damage leading to an increase in lipid peroxidation, protein oxidation and alteration of enzyme activity antioxidant.

EFFECT OF HISTAMINE ON GASTRIC PEPSINOGEN, PEPSIN SECRETION, AND BLOOD PEPSINOGEN IN GUINEA PIGS

1957 ◽  
Vol 35 (1) ◽  
pp. 729-732
Author(s):  
K. Kowalewski
Keyword(s):  
Body Weight ◽  
Gastric Mucosa ◽  
Guinea Pig ◽  
Gastric Juice ◽  
Guinea Pigs ◽  
Gastric Tissue ◽  
Pepsin Secretion ◽  
Enzymatic Function ◽  
Guinea Pig Stomach

The endocrine and exocrine activity of guinea pig stomach was measured by the determination of pepsinogen in gastric tissue and in plasma. Gastric juice pepsin was also studied.A significant increase of both pepsinogen and pepsin was found in animals treated with a dose of histamine (75 mg. per kg. of body weight). These results give further evidence that the zymogenic cells of gastric mucosa may be stimulated by histamine. The determination of pepsinogen in gastric tissue seems to permit a direct approach to the enzymatic function of zymogenic cells.

A STUDY ON THE RELATIONSHIP BETWEEN HELICOBACTER PYLORI AND TRACE ELEMENTS IN GASTRIC MUCOSA

2006 ◽  
Vol 16 (01n02) ◽  
pp. 55-68
Author(s):  
T. FUKUDA ◽  
S. SAKURAI ◽  
M. KUDO
Keyword(s):  
Helicobacter Pylori ◽  
Trace Elements ◽  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Gastric Ulcers ◽  
Control Group ◽  
Human Gastric Mucosa ◽  
H2 Receptor Antagonist ◽  
Zinc Levels ◽  
The Relationship

H2 receptor antagonist (H2RA) and proton pump inhibitor(PPI) are often used to treat gastric ulcers, in addition to the zinc-L-carcino-complex which is commonly adopted for such therapies. However, there have been no previous reports detailing the levels and distribution of zinc in human gastric mucosa. The aim of our current study is to clarify the relationship between Helicobacter pylori infection and trace elements such as zinc in gastric mucosa using particle induced X-ray emission (PIXE). Forty gastric ulcer patients were chosen as subjects of this study and divided into two groups. One is a new ulcer patients group(twenty nine cases). And the other, eleven of these patients had been prescribed zinc-L-carcino-complex (polapreZinc®), at a dose of 150mg/day for periods ranging from 16 to 20 weeks. The zinc levels in the gastric ulcer group were found to be significantly less than those of the control group and the concentrations of zinc in gastric mucosa were observed to increase following the administration of treatments containing this element.

scholarly journals Effects of Grape Seed Extract, Vitamin C, and Vitamin E on Ethanol- and Aspirin-Induced Ulcers

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Vivian Molina Cuevas ◽  
Yazmín Ravelo Calzado ◽  
Yohani Pérez Guerra ◽  
Ambar Oyarzábal Yera ◽  
Sonia Jiménez Despaigne ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Vitamin E ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Gastric Ulcers ◽  
Control Group

Effects of GSE and vitamins C and E on aspirin- and ethanol-induced gastric ulcer and associated increases of lipid peroxidation in rats were compared. Two experiments were conducted. Rats were randomized into eight groups: a negative control and seven groups that received aspirin or ethanol for ulcer induction: one positive control (vehicle) and six with VC, VE, or GSE (25 and 250 mg/kg). Ulcer indexes and gastric levels of malondialdehyde (MDA) were quantified. VC, VE, and GSE (25 and 250 mg/kg) decreased aspirin, and ethanol-induced ulcers and MDA values compared with positive control group. The magnitude of aspirin ulcer reduction was comparable for all treatments, and MDA decrease with GSE was higher than with VC and tended to be greater, albeit none significantly, than with VE. GSE was more effective than VC and VE for lowering the ethanol ulcers, while the decrease of MDA levels with GSE was greater than with VC, but comparable to that achieved with VE. GSE protected against ethanol-induced gastric ulcers more effectively than VC or VE, while its protection against aspirin ulcers was comparable for all treatments. GSE produced the greatest reductions of gastric MDA in both models.

scholarly journals Costus afer (Costaceae, Zingiberales) leaf extract ameliorates naproxen-induced gastric ulcer in rats

2021 ◽  
Vol 19 (12) ◽  
pp. 2615-2622
Author(s):  
Hala AH Khattab ◽  
Buthaina M. Aljehany
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Gastric Acidity ◽  
Leaf Extract ◽  
Reduced Glutathione ◽  
Lipid Peroxide ◽  
Gastric Ph ◽  
Ulcer Index ◽  
Costus Afer

Purpose: To examine Costus afer leaf extract (CALE) protective effects against naproxen-induced gastric ulcer and the mechanisms of protection.Methods: Rats with naproxen-induced ulcer were pretreated with either CALE (800 mg/kg), pantoprazole (20 mg/kg), or a combination of both. Then the ulcer index, total gastric acidity, gastric pH, and curative index were evaluated. In addition, gastric mucin, pepsin, prostaglandin E2, nitric oxide, reduced glutathione, lipid peroxide, and superoxide dismutase were quantified. The gastric pathologicalchange was also evaluated.Results: Rats treated with CALE, pantoprazole, and their combination significantly decreased ulcer index, total gastric acidity, and gastric pH. All treatments induced a significant curative index in favor of the combination. The CALE significantly increased gastric mucin, prostaglandin E2, nitric oxide, reduced glutathione, and superoxide dismutase. However, the CALE significantly decreased pepsin and lipid peroxide product.Conclusion: These results reveal that CALE protects the stomach against naproxen-induced ulcer. This action is linked to increased gastroprotective factors, increased antioxidants, and decreased lipid peroxidation. The CALE may be used as an adjunctive treatment for ulcers caused by NSAIDs. Keywords: Costus afer, Naproxen, Gastric ulcer, Antioxidants, Lipid peroxidation

Anti-ulcerogenic effect of the methanol extract of Chasmanthera dependens (Hochst) stem on male Wistar rats

2018 ◽  
Vol 29 (4) ◽  
pp. 377-383 ◽  
Author(s):  
Stephanie Abiola Tijani ◽  
Samuel B. Olaleye ◽  
Ebenezer O. Farombi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Antioxidant System ◽  
Methanol Extract ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Standard Drug

AbstractBackgroundOxidative stress and free radical-mediated processes have been implicated in the pathogenesis of indomethacin-induced gastric ulcer. This study investigated the ability of the methanol extract ofChasmanthera dependensto protect the gastric mucosal from oxidative damage induced by oral administration of indomethacin in rats.MethodsTheC. dependensstems were chopped into pieces, air-dried, and pulverized into powder. One kilogram of the powder was macerated in 1 L of methanol for 72 h. The mixture was filtered and evaporated using rotatory evaporator to obtain the extract ofC. dependens. Adult male rats were divided into eight groups of six animals per group and were pretreated orally with the methanol extract ofC. dependens(200, 400, and 800 mg/kg) or cimetidine (CIM), a standard drug (50 mg/kg), for 7 days. Gastric ulcer was induced orally with indomethacin. Ulcerogenic parameters, oxidative stress indices, and histopathological examination of the stomach were assessed to monitor the gastroprotective potential ofC. dependensstem.ResultsIndomethacin caused severe gastric mucosa damage and significant reduction in the gastric mucosa antioxidant system with concomitant increase in the level of lipid peroxidation. Pretreatment with the methanol extract ofC. dependensor CIM significantly reduced the formation of ulcer at the different doses administered. Similarly, pretreatments with the extract or CIM improved the antioxidant system, decreased acid output, lipid peroxidation, and improved the architecture of the gastric mucosa in ulcerated rats.ConclusionsThe results show the gastroprotective effect of the methanolic extract ofC. dependens, which may be attributed to its antioxidant properties.

scholarly journals Cardioprotective Potential of Murraya koenigii (L.) Spreng. Leaf Extract against Doxorubicin-Induced Cardiotoxicity in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-16 ◽  
Author(s):  
Jayasinghe A. N. Sandamali ◽  
Ruwani P. Hewawasam ◽  
Kamani A. P. W. Jayatilaka ◽  
Lakmini K. B. Mudduwa
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Leaf Extract ◽  
Troponin I ◽  
Control Plant ◽  
Treated Group ◽  
Free Radical Scavengers ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Murraya Koenigii

Dose-dependent cardiotoxicity of doxorubicin may lead to irreversible congestive heart failure. Although multiple mechanisms are involved, generation of free radicals is the most commonly postulated mechanism. Therefore, free radical scavengers are considered as potential therapeutic agents. As Murraya koenigii leaves are a rich source of flavonoids and phenols, they have the ability to scavenge free radicals effectively. Therefore, the objective of this study was to investigate the cardioprotective potential of Murraya leaf extract against doxorubicin-induced cardiotoxicity in rats. Rats were randomly divided into five groups with 10 animals in each group. Doxorubicin was administered intraperitonially at 18 mg/kg while lyophilized plant extract was administered orally at 2 g/kg. Dexrazoxane, at 180 mg/kg, was used as the positive control. Cardiac damage of doxorubicin control was evident with a significant increase (p<0.05) in cardiac troponin I, NT-pro BNP, AST, and LDH compared to the normal control. Plant-treated group showed cardioprotective effect by significantly reducing (p < 0.05) all of the above parameters compared to doxorubicin control (p<0.05). Increased oxidative stress in doxorubicin control was evident with a significant reduction in reduced glutathione, glutathione reductase, glutathione peroxidase, total antioxidant capacity, superoxide dismutase, and catalase activity and a significant increase in lipid peroxidation compared to the control. Interestingly, treatment with Murraya leaf extract showed a significant increase in all of the above antioxidant parameters and a significant reduction in lipid peroxidation by showing an antioxidant effect. A significant increase in myeloperoxidase activity confirmed the increased inflammatory activity in doxorubicin control group whereas plant-treated group showed a significant reduction (p<0.05) which expressed the anti-inflammatory effect of Murraya leaf extract. Doxorubicin-treated group showed histological evidence of extensive damage to the myocardium while plant-treated group showed a preserved myocardium with lesser degree of damage. Pretreatment with Murraya leaf extract may replenish cardiomyocytes with antioxidants and promote the defense against doxorubicin-induced cardiotoxicity.

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