Protective effects of dietary selenium and vitamin C in barium-induced cardiotoxicity

2016 ◽  
Vol 36 (11) ◽  
pp. 1146-1157 ◽  
Author(s):  
Awatef Elwej ◽  
Imen Ghorbel ◽  
Mariem Chaabane ◽  
Nejla Soudani ◽  
Rim Marrekchi ◽  
...  
Keyword(s):  
Vitamin C ◽  
Environmental Pollutants ◽  
Density Lipoprotein ◽  
Barium Chloride ◽  
Antioxidant Defense System ◽  
Protein Product ◽  
Diet Group ◽  
Lipoprotein Cholesterol ◽  
Protective Effects ◽  
Adult Rats

Several metals including barium (Ba) known as environmental pollutants provoke deleterious effects on human health. The present work pertains to the potential ability of selenium (Se) and/or vitamin C, used as nutritional supplements, to alleviate the toxic effects induced by barium chloride (BaCl2) in the heart of adult rats. Animals were randomly divided into seven groups of six each: group 1, serving as negative controls, received distilled water; group 2 received in their drinking water BaCl2 (67 ppm); group 3 received both Ba and Se (sodium selenite 0.5 mg kg−1 of diet); group 4 received both Ba and vitamin C (200 mg kg−1 bodyweight) via force feeding; group 5 received Ba, Se, and vitamin C; and groups 6 and 7, serving as positive controls, received either Se or vitamin C for 21 days. The exposure of rats to BaCl2 caused cardiotoxicity as monitored by an increase in malondialdehyde, hydrogen peroxide, and advanced oxidation protein product levels, a decrease in Na+-K+ adenosine triphosphatase (ATPase), Mg2+ ATPase, and acetylcholinesterase activities and in antioxidant defense system (catalase, glutathione peroxidase, superoxide dismutase, glutathione, and nonprotein thiols). Plasma lactate dehydrogenase and creatine kinase activities, total cholesterol, triglyceride, and low-density lipoprotein–cholesterol levels increased, while high-density lipoprotein–cholesterol level decreased. Coadministration of Se and/or vitamin C restored the parameters indicated above to near control values. The histopathological findings confirmed the biochemical results. Se and vitamin C may be a promising therapeutic strategy for Ba-induced heart injury.

PROTECTIVE EFFECTS OF VITAMIN C AGAINST CISPLATIN-INDUCED NEPHROTOXICITY AND LIPID PEROXIDATION IN ADULT RATS: A DOSE-DEPENDENT STUDY

2000 ◽  
Vol 41 (4) ◽  
pp. 405-411 ◽  
Author(s):  
LUSÂNIA M. GREGGI ANTUNES ◽  
JOANA D'ARC C. DARIN ◽  
MARIA DE LOURDES P. BIANCHI
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin C ◽  
Protective Effects ◽  
Adult Rats ◽  
Dose Dependent

Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats

2016 ◽  
Vol 54 (6) ◽  
pp. 964-974 ◽  
Author(s):  
Awatef Elwej ◽  
Ghada Ben Salah ◽  
Choumous Kallel ◽  
Faiza Fakhfakh ◽  
Najiba Zeghal ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Barium Chloride ◽  
Protective Effects ◽  
Pomegranate Peel ◽  
Adult Rats

Effects of acrylamide graded doses on metallothioneins I and II induction and DNA fragmentation: Bochemical and histomorphological changes in the liver of adult rats

2017 ◽  
Vol 33 (8) ◽  
pp. 611-622 ◽  
Author(s):  
Imen Ghorbel ◽  
Awatef Elwej ◽  
Mariem Chaabene ◽  
Ons Boudawara ◽  
Rim marrakchi ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Protein Carbonyl ◽  
Lipoprotein Cholesterol ◽  
Dependent Manner ◽  
Adult Rats ◽  
Glucose Levels ◽  
Hepatic Cells ◽  
Genes Expression ◽  
Histomorphological Changes

The present study investigates the toxic effects of acrylamide (ACR) administered to rats at two doses on (i) oxidative stress and disruption of pro-oxidant/antioxidant balance in hepatic cells and (ii) its correlation with metallothioneins (MTs) genes expression, DNA damage and histomorphological changes. Treated rats with 20 and 40 mg/kg body weight of ACR led to an increase in malondialdehyde, hydrogen peroxide, advanced oxidation protein products, protein carbonyl levels as well as an alteration in the antioxidant status. Total MT content in the liver and MT I and MT II genes induction were increased. Plasma transaminases activities, albumin, total protein and glucose levels were also increased, while alkaline phosphatase activity was decreased. Moreover, total cholesterol (TC), triglyceride, low-density lipoprotein cholesterol (LDL-C) levels, TC/high-density lipoprotein cholesterol (HDL-C) and LDL-C/HDL-C ratios were increased, while HDL-C decreased in a dose-dependent manner. A random DNA degradation was observed only in the liver of ACR-treated rats with the highest dose. These changes were confirmed by histopathological observations.

scholarly journals The Underlying Mechanisms of Curcumin Inhibition of Hyperglycemia and Hyperlipidemia in Rats Fed High-fat Diet or a High-fat Diet Combined with STZ Treatment

2019 ◽  
Author(s):  
Zhen-hong Xia ◽  
Wen-bo Chen ◽  
Li Shi ◽  
Xue Jiang ◽  
Ke Li ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Curcuma Longa ◽  
Food Pellet ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diet Group ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat

Curcumin is the main secondary metabolites of Curcuma longa and other Curcuma spp, and has been reported to have some potential in preventing and treating some physiological disorders. This study investigated the effect curcumin in inhibiting high-fat diet and streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Twenty-six male Sprague-Dawley (SD) rats (170-190 g) were randomly divided into a standard food pellet diet group (Control group), a high-fat diet and streptozotocin group (HF+STZ group), and a high-fat diet combined with curcumin and STZ group (HF+ Cur +STZ group). Compared with the HF+STZ group, the HF+Cur+STZ group exhibited significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (AST) and aspartate transaminase (ALT) levels, and liver coefficients; in the livers of these rats, the expression of malondialdehyde (MDA) and Bax was downregulated, whereas that of superoxide dismutase (SOD) and Bcl-2 was upregulated. Moreover, the liver histology of these rats was improved and resembled that of the control rats. These results suggest that curcumin prevents high-fat diet and STZ-induced hyperglycemia and hyperlipidemia, mainly via anti-oxidant and anti-apoptotic mechanisms in the liver.

Fasting is not required for measuring plasma lipid levels in rabbits

2019 ◽  
Vol 54 (3) ◽  
pp. 272-280 ◽  
Author(s):  
Rong Wang ◽  
Ruihan Liu ◽  
Lu Li ◽  
Baoning Liu ◽  
Liang Bai ◽  
...  
Keyword(s):  
Corn Oil ◽  
Experimental Studies ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Diet Group ◽  
Lipoprotein Cholesterol ◽  
High Cholesterol Diet ◽  
Chow Diet ◽  
Lipid Levels ◽  
Glucose Levels

Plasma lipid and glucose levels are important parameters for evaluating the onset and development of metabolic and cardiovascular diseases. In clinical and experimental studies of humans or mice, fasting is often required before testing plasma lipid and glucose levels. The rabbit is a valuable animal model for cardiovascular disease research. However, whether fasting is necessary for measuring plasma lipid and glucose levels in rabbits remains unclear. In the current study, 12 healthy Japanese white rabbits (males weighing 2.5–3.0 kg) were randomly divided into a chow diet group ( n = 6) and a high cholesterol diet group ( n = 6). They were fed either a standard chow diet or a chow diet supplemented with 0.5% cholesterol and 3% corn oil for 12 weeks. After 12 weeks, the plasma levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and glucose were measured before and after various fasting durations (8, 12, 16, 20 and 24 h). The results showed that there were no significant differences in lipid levels between the fasting and non-fasting samples, whereas glucose levels were lower after 8 h of fasting than in the absence of fasting. Moreover, the glucose levels were restored to normal after 8 h of refeeding. These results indicate that fasting does not affect plasma lipid values in rabbits but that fasting is important for determining the glucose level in rabbits. These findings may be helpful for future rabbit experiments and beneficial for animal welfare.

scholarly journals The Underlying Mechanisms of Curcumin Inhibition of Hyperglycemia and Hyperlipidemia in Rats Fed a High-Fat Diet Combined With STZ Treatment

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 271 ◽  
Author(s):  
Zhen-Hong Xia ◽  
Wen-Bo Chen ◽  
Li Shi ◽  
Xue Jiang ◽  
Ke Li ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Curcuma Longa ◽  
Food Pellet ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diet Group ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat

Curcumin is the main secondary metabolite of Curcuma longa and other Curcuma spp, and has been reported to have some potential in preventing and treating some physiological disorders. This study investigated the effect of curcumin in inhibiting high-fat diet and streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Twenty-six male Sprague-Dawley (SD) rats (170–190 g) were randomly divided into a standard food pellet diet group (Control group), a high-fat diet and streptozotocin group (HF + STZ group), and a high-fat diet combined with curcumin and STZ group (HF + Cur + STZ group). Compared with the HF + STZ group, the HF + Cur + STZ group exhibited significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (AST), and aspartate transaminase (ALT) levels, as well as liver coefficients. In the livers of these rats, the expression of malondialdehyde (MDA) and Bax was downregulated, whereas that of superoxide dismutase (SOD) and Bcl-2 was upregulated. Moreover, the liver histology of these rats was improved and resembled that of the control rats. These results suggest that curcumin prevents high-fat diet and STZ-induced hyperglycemia and hyperlipidemia, mainly via anti-oxidant and anti-apoptotic mechanisms in the liver.

Protective effects of vitamin C and selenium supplementation on methomyl-induced tissue oxidative stress in adult rats

2012 ◽  
Vol 31 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Assia Djeffal ◽  
Mahfoud Messarah ◽  
Amel Boumendjel ◽  
Lilia Kadeche ◽  
Abdelfattah El Feki
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Selenium Supplementation ◽  
Protective Effects ◽  
Adult Rats

scholarly journals Mitigation Effects of Vitamin C and E Against Copper Toxicity in Broiler Chickens, With Reference to Biochemical, Genotoxicity and Pathological Studies

2021 ◽  
Author(s):  
Mohamed Hashem ◽  
Sahar Abd El Hamied ◽  
Eman Ahmed
Keyword(s):  
Vitamin C ◽  
Broiler Chickens ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Feed Consumption ◽  
Control Group ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Vit C

Abstract Copper (Cu) is necessary for biological utility, nevertheless when existing in abundance; it can produce plentiful injurious impacts. This enquiry was carried out to explore the efficiency of individual or combined doses of vitamin C (Vit C) and vitamin E (Vit E) in ameliorating some biochemical, genotoxicity and pathological changes in the liver of copper sulphate (CuSO 4 )-intoxicated chickens. One hundred- one day old broiler chicks were haphazardly divided into 5groups of 20 chicks each. The broilers were fed on basal diet only (control, gp.1 ) or supplemented with 300 mg CuSO 4 /kg diet (Cu, gp.2 ), CuSO 4 + 250 mg Vit C /kg diet (Cu+ Vit C, gp.3 ), CuSO 4 +250 mg Vit E /kg diet (Cu+ Vit E, gp.4 ) and both vitamins C + E (Cu+ Vit C+ Vit E, gp.5 ) for six weeks. The results displayed that CuSO 4 - intoxicated birds (gp.2) had significantly ( p<0.05 ) dwindled body weight, gain and feed consumption with increased feed conversion rate from week 2 till the 6 th week compared with control group. Serum aminotransferases (ALT, AST), and alkaline phosphatase (ALP) were significantly ( p<0.05 ) augmented in CuSO4- exposed group (gp.2) with significantly ( p<0.05 ) drop in serum total protein (TP), albumin, globulins, triglycerides (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C), and high density lipoprotein-cholesterol (HDL-C) levels compared to control. Concomitantly, histopathological and DNA changes were perceived in liver of CuSO 4 -intoxicated birds. Co-supplementation of Vit C, and Vit E single-handedly or incorporation to CuSO 4 -intoxicated chickens displayed an enhancement in performance traits and abovementioned changes, especially with those given combination of vitamins. From the extant enquiry, it could be established that supplementation of vitamin C and E were beneficial for alleviation the harmful effects of CuSO 4 on performance and other studied parameters in broiler chickens.

scholarly journals Proanthocyanidins Protect Against Cadmium-Induced Diabetic Nephropathy Through p38 MAPK and Keap1/Nrf2 Signaling Pathways

2022 ◽  
Vol 12 ◽  
Author(s):  
Pin Gong ◽  
Peipei Wang ◽  
Sihui Pi ◽  
Yuxi Guo ◽  
Shuya Pei ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
P38 Mapk ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Protective Mechanism ◽  
Protective Effects ◽  
Antioxidative Status

Diabetic nephropathy (DN) is one of the most devastating complications of diabetes mellitus. Although cadmium (Cd) exposure might be involved in the pathogenesis of DN, the underlying mechanism is still unclear. In this study, we explored the protective effects and possible mechanism of proanthocyanidins (OPC) from grape seed using a mouse model of Cd-induced DN. The successful establishment of this model was verified by analyzing the physiological and biochemical indices of mice, including their body weight and tissue ratio; levels of blood glucose, creatinine, microalbumin, total cholesterol, triglycerides, high-density lipoprotein-cholesterol and low-density lipoprotein-cholesterol; and was based on histopathological examination. Oxidative-antioxidative status, elemental analysis, and key signaling pathway analysis were performed to explore the possible protective mechanism of OPC. The protective effects of OPC and its possible mechanism in preventing the progression of DN were investigated using a multidimensional approach, including its ability in regulating oxidative-antioxidative status (lipid peroxidation, protein carbonyl, superoxide dismutase, and glutathione GST, GSH-Px), metal-binding ability (Cd levels in the kidneys and urine and MT content) and mediation of essential elements (Zn, Ca, Cu, and Fe levels in the kidneys), and activation of the p38 MAPK and Keap1/Nrf2 signaling pathways. OPC exhibited a significant renoprotective effect, attributed to the metal-chelating ability, anti-oxidative effect, and mediation of oxidative stress-related signaling pathway. These results highlight the potential of OPC in preventing or treating DN in humans and suggest the dietary intake of grapes, which are rich in polyphenols, for the prevention of type 2 diabetes mellitus and its complications.

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