Effects of Achillea tenuifolia Lam. hydro-alcoholic extract on anxiety-like behavior and reproductive parameters in rat model of chronic restraint stress

2021 ◽  
pp. 096032712110267
Author(s):  
Y Bagheri ◽  
E Fathi ◽  
A Maghoul ◽  
S Moshtagh ◽  
K Mokhtari ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Restraint Stress ◽  
Sperm Quality ◽  
Reproductive Capacity ◽  
Male Rats ◽  
Alcoholic Extract ◽  
Chronic Restraint Stress ◽  
Reproductive Parameters ◽  
Behavioral Impairment ◽  
The Impact

Introduction: Achillea tenuifolia Lam ( AT) has several biological activities and medicinal properties. In this study, we elucidated the impact of the AT on anxiety-related behaviors, reproductive parameters, antioxidant capacity in male rats subjected to chronic restraint stress (CRS). Methods: 35 Wistar rats were divided into five groups: control, CRS-control (received normal saline) and three CRS-treated groups received AT extract (100, 150, and 200 mg/kg body weight) for 21 consequences days. To induce CRS rats, the rats were immobilized for 21 days and received the extract orally. On the last day of treatment, anxiety-related behaviors were assessed through the sucrose preference test (SPT) as well as elevated plus maze (EPM) tests. Corticosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), testosterone levels were evaluated to determine reproductive capacity. Sperm parameters including the total count, motility, and viability were also analyzed. Weight of body, testis and seminal vesicles was measured as well. Results: The findings revealed that 100, 150, and 200 mg/kg of AT extract had anxiolytic effects in CRS rats, as confirmed by the EPM test and SPT. In addition, AT extract could improve fertile capacity and sperm quality to varying degrees. The level of corticosterone had decreased, whereas the level of LH, FSH and testosterone had increased in CRS-treated rats. Moreover, the reduced level of MDA coincided with an increased rate of antioxidant capacity. Our findings suggest that AT extract could alleviate stress-induced dysfunctions. Conclusion: Overall, these observations would infer that AT extract could improve fertility capacity and behavioral impairment in the stress conditions. Graphical abstract: Assumption pathway describing the probability underlying mechanism of CRS-induced anxiety and reproductive toxicity and protective effect of AT.

Effects of chronic restraint stress on social behaviors and the number of hypothalamic oxytocin neurons in male rats

Neuropeptides ◽  
2016 ◽  
Vol 60 ◽  
pp. 21-28 ◽  
Author(s):  
Jin Li ◽  
Han-Xia Li ◽  
Xiao-Jing Shou ◽  
Xin-Jie Xu ◽  
Tian-Jia Song ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Social Behaviors ◽  
Male Rats ◽  
Chronic Restraint Stress

Maternal restraint stress during pregnancy negatively affects behaviors and antioxidant capacity of offspring rats (Rattus norvegicus)

2018 ◽  
Vol 96 (8) ◽  
pp. 882-887 ◽  
Author(s):  
Chao Yin ◽  
Guofu Wang ◽  
Shixing Gao ◽  
Yanping Huang ◽  
Ruqian Zhao ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Soleus Muscle ◽  
Rattus Norvegicus ◽  
Biochemical Parameters ◽  
Restraint Stress ◽  
Gastrocnemius Muscle ◽  
Female Offspring ◽  
Female Rats ◽  
Male Rats ◽  
Biochemical Indices

This study evaluated the effect of maternal restraint stress during the gestation period on behaviors, biochemical parameters, and antioxidant capacities of offspring rats (Rattus norvegicus (Berkenhout,1769)) at weaning age. Behaviors, plasma biochemical indices, and antioxidant ability of the liver, soleus muscle, and gastrocnemius muscle of mother and (or) offspring rats were analyzed. Significant increases were found in the immobility and swinging behavior frequencies of offspring male rats; no difference was found in behaviors of female rats. The antioxidant indices including superoxide dismutase, nitric oxide synthase, and total antioxidant capacity in the soleus muscle of offspring male rats were significantly decreased in the restraint group. Female offspring rats showed significant lower glutathione and higher malondialdehyde levels in the gastrocnemius muscle and liver, respectively. No difference was found in the productive performance and plasma biochemical indices of maternal rats, nor in the biochemical parameters of the two groups of weaning rats. The results suggested that maternal chronic stresses negatively affected the behaviors and antioxidant abilities of offspring rats, and that these effects possibly have a greater impact on offspring male rats than on female rats.

Effects Of Vitis Vinifera Leave Hydro-Alcoholic Extract On Reproductive Parameters In Adult Normal Male Rats.

2013 ◽  
Vol 3 (6) ◽  
pp. 159
Author(s):  
Afzalzadeh Reza ◽  
Amirzargar Ashraf ◽  
Ahangarpour Akram ◽  
KazemiVarnamkhasti Mohammad ◽  
Ganjali Hadi ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Male Rats ◽  
Normal Male ◽  
Alcoholic Extract ◽  
Reproductive Parameters

scholarly journals Vitamin D Regulatory Effect on Restraint Stress-Induced Changes on Serum Corticosterone and Hippocampus BDNF Levels in Male Rats

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Katayoun Sedaghat ◽  
Sara Choobdar ◽  
Ahmad Reza Bandegi ◽  
Zahra Ghods
Keyword(s):  
Vitamin D ◽  
Chronic Stress ◽  
Restraint Stress ◽  
Male Rats ◽  
Negative Effects ◽  
Bdnf Level ◽  
Serum Corticosterone ◽  
Protein Levels ◽  
Induced Changes ◽  
The Impact

Background: Chronic stress exerts negative effects on cognitive functions through inducing changes in the hippocampus. Brain-derived neurotrophic factor (BDNF) is an essential factor in cognitive activities, which is considerably reduced under chronic stress. 1,25(OH)2 vitamin D plays neuroprotective roles partially by regulating the expression of various neurotrophic factors. Objectives: Since few studies have studied the impact of vitamin D on BDNF level, we conducted this brief experiment to understand the role of vitamin D in maintaining hippocampal BDNF protein levels by using restraint as a model of chronic stress in rats. Methods: Rats underwent restraint stress 3 h/day for 28 days, during which they received vitamin D (5, 10 μg/kg) or its vehicle (IP, twice weekly). After the stress period, serum corticosterone (CORT) and hippocampus BDNF protein levels were measured. Results: Restraint stress increased serum CORT (P < 0.001) and reduced BDNF protein levels (P < 0.001) as compared to the non-stress group. Vitamin D markedly maintained BDNF level close to normal (P < 0.001), but did not change CORT level significantly. Conclusions: This study demonstrated that 3h/day of chronic restraint stress for 28 days boosted serum CORT and declined hippocampal BDNF levels, similar to stronger restraint stress models. Vitamin D maintained BDNF level close to normal in the hippocampus, but it did not affect CORT level significantly.

scholarly journals Intraventricular T3 reverses chronic restraint stress-induced depressive-like behaviors: Inhibition of NF-κB/ NLRP3 inflammasome pathway in the hippocampus

2021 ◽  
Author(s):  
Tahmineh Mokhtari ◽  
AymanEl-Meghawry El-Kenawy ◽  
Li Hu
Keyword(s):  
Nlrp3 Inflammasome ◽  
Restraint Stress ◽  
Male Rats ◽  
Control Group ◽  
Therapeutic Effects ◽  
Chronic Restraint Stress ◽  
Model Group ◽  
Nissl Staining ◽  
Caspase 1 ◽  
Ca1 Region

Abstract In this study, the effects of triiodothyronine (T3) were evaluated on the NLR family pyrin domain containing 3 (NLRP3) inflammasome complex formation in the rat's hippocampus with restraint stress-induced depressive-like behaviors.Thirty-six Wistar male rats were randomly allocated to following groups: Control, Model, and Model + T3. In the Model or Model+T3 group, a single dose of PBS or T3 was administered into the lateral ventricle. Depressive-like behaviors were induced by chronic restraint stress. The forced swimming (FST), tail suspension (TST), and open field (OFT) tests were used to investigate the depression. The rats were sacrificed, and brain tissues were stored for molecular and pathological evaluations. Chronic stress increased the immobility of rats in the Model group according to FST, TST, and OFT (P < 0.05). T3 significantly improved depressive-like behaviors (P < 0.05). The gene expression and protein level of hippocampal nuclear factor kappa B (NF-κB), NLRP3, apoptosis-associated speck-like protein (ASC), and Caspase-1 significantly increased in the Model group compared to the control group (P < 0.05). The reduced hippocampal levels of NF-κB, NLRP3, ASC, and Caspase-1 were observed in the T3 group compared to the Model group (P < 0.05). The Nissl staining of the CA1 region showed an increased number of dark neurons (P < 0.05) and reduced pyramidal layer thickness (P < 0.05) in the Model group. These histopathological alterations were changed by T3 administration compared to the Model group (P < 0.05). The findings confirmed the therapeutic effects of intraventricularly T3 on depressive-like behaviors induced by restraint stress via surviving pyramidal neurons of the CA1 region and inhibition of NF-κB/NLRP3 inflammasome pathway.

scholarly journals Non Concurrent Multimodal Stress Decreases Sperm Quality and Motor Activity in Male Wister Albino Rats

2019 ◽  
Vol 12 (3) ◽  
pp. 1505-1517 ◽  
Author(s):  
Jyothi Ashok Kumar ◽  
Thotakura Balaji ◽  
C. Swathi Priyadarshini ◽  
Manickam Subramanian ◽  
Indumathi Sundaramurthi
Keyword(s):  
Body Weight ◽  
Muscle Strength ◽  
Motor Activity ◽  
Sperm Quality ◽  
Elevated Serum ◽  
Male Reproduction ◽  
Male Rats ◽  
Albino Rats ◽  
Serum Corticosterone ◽  
The Impact

Every human being is exposed to the stress in one or the other form in the day to day life. Most of the existing studies on the impact of stress on the male reproduction were assessed by using single stressor, which may lead habituation to that stressor. The present study intends to estimate the consequence of stress on motor activity, sperm quality and histopathology of the testis in stress-induced male rats using multimodal stress one per day. Four weeks old Wister albino rats were randomly split into 4 groups and induced multimodal stress at different ages of life span. After induction of stress serum corticosterone levels, muscle strength and coordination, quality of sperm and histopathology of testes were estimated. Elevated serum corticosterone levels and body weight, reduced muscle strength, coordination. Sperm concentration and motility was significantly reduced and increased morphologically abnormal sperm in stress induced animals but sperm viability was not altered much. Histopathology of testes in stress received animals showed decreased tubular diameter and increased intertubular space. Multimodal stress caused elevated serum corticosterone and body weight, decreased motor activity, sperm quality and degenerative changes in the testis

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