scholarly journals Whole Blood Selenium Levels and Selenium Supplementation in Patients Treated in a Family Doctor Practice in Golßen (State of Brandenburg, Germany): A Laboratory Study

2018 ◽  
Vol 17 (4) ◽  
pp. 1132-1136 ◽  
Author(s):  
Ralph Muecke ◽  
Knut Waldschock ◽  
Lutz Schomburg ◽  
Oliver Micke ◽  
Jens Buentzel ◽  
...  
Keyword(s):  
Laboratory Study ◽  
Whole Blood ◽  
Family Doctor ◽  
Selenium Supplementation ◽  
Patients With Cancer ◽  
Negative Effect ◽  
Se Deficiency ◽  
Student’S T ◽  
Se Uptake ◽  
Student’S T Test

Introduction: The supply of selenium (Se) varies widely in Germany. Therefore, a laboratory study was conducted in patients treated at a family doctor practice in Brandenburg, Germany, to determine whether there is a general Se deficiency in this area; specifically, whether Se concentrations differ with age, sex, or presence of cancer. Moreover, we tested the effects of a Se supplementation on whole blood Se levels (WBSL). Methods: In 2006, WBSL were analyzed in 871 patients (496 females, 375 males, median age: 67 years). Of these, 143 (78 females, 65 males) had cancer and were in an aftercare situation. From 2006 to 2012, 317 patients (76 with tumors, 241 without tumors) received continuous Se supplementation with sodium selenite (300 µg per day) and annual WBSL measurements. WBSL were compared by Student’s t test for paired and independent samples. Results: The initial WBSL of all patients was 97.2 ± 20.7 µg/L (mean ± SD). WBSL did not differ with regard to age or sex, but patients with cancer had the lowest WBSL. Se supplementation increased mean WBSL in both patients with (to 128.5 µg/L) and without (to 119.52 µg/L) cancer ( P < .001). Discussion: Patients with cancer displayed significantly lower WBSL than patients without cancer, indicating a negative effect of tumors on Se uptake, absorption, or metabolism. Significant influences of age or sex were not observed. Selenite supplementation efficiently improved WBSL to concentrations considered necessary for health benefits.

Metabolomic Heterogeneity of Urogenital Tract Cancers Analyzed by Complementary Chromatographic Techniques Coupled with Mass Spectrometry

2019 ◽  
Vol 26 (1) ◽  
pp. 216-231 ◽  
Author(s):  
Arlette Yumba-Mpanga ◽  
Wiktoria Struck-Lewicka ◽  
Renata Wawrzyniak ◽  
Marcin Markuszewski ◽  
Marek Roslan ◽  
...  
Keyword(s):  
Tricarboxylic Acid Cycle ◽  
Principal Component ◽  
The United States ◽  
Urogenital Tract ◽  
Control Group ◽  
Tract Cancer ◽  
Patients With Cancer ◽  
Student’S T ◽  
Cancer Studies ◽  
Student’S T Test

Background: In regard to urogenital tract cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system cancer and about 141,140 new cases and 29330 deaths from urinary system were projected to occur in the United States in 2012. The main drawbacks of currently available diagnostic tests constitute the low specificity, costliness and quite high invasiveness. Objective: The main goal of this pilot study was to determine and compare urine metabolic fingerprints in urogenital tract cancer patients and healthy controls. Method: A comparative analysis of the metabolic profile of urine from 30 patients with cancer of the genitourinary system (bladder (n=10), kidney (n=10) and prostate (n=10)) and 30 healthy volunteers as a control group was provided by LC-TOF/MS and GCQqQ/ MS. The data analysis was performed by the use of U-Mann Whitney test or Student’s t-test, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). Results: As a result, 33, 43, and 22 compounds were identified as statistically significant in bladder, prostate and kidney cancer, respectively, compared to healthy groups. Conclusion: Diverse compounds such as purine, sugars, amino acids, nucleosides, organic acids which play a role in purine metabolism, in tricarboxylic acid cycle, in amino acid metabolism or in gut microbiota metabolism were identified. Only two metabolites namely glucocaffeic acid and lactic acid were found to be in common in studied three types of cancer.

scholarly journals Clotting Studies Performed on Blood Stored in Half-Strength ACD-A

1977 ◽  
Author(s):  
S. Cederholm-Williams ◽  
J.M. Mishler ◽  
J.H. Darley
Keyword(s):  
Citric Acid ◽  
Whole Blood ◽  
Trisodium Citrate ◽  
Thrombin Time ◽  
Healthy Male ◽  
Clotting Time ◽  
Solution Ph ◽  
Half Strength ◽  
Student’S T ◽  
Student’S T Test

Six hundred ml of whole blood from each of five healthy male donors was equally divided and stored at 4-6°C in either standard ACD-A (2.2g trisodium citrate, 0.8g citric acid, 2.45g dextrose/dl anticoagulant solution, pH 4.9) or half-strength ACD-A (l.lg trisodium citrate, 0.8g citric acid, 2.45g dextrose, pH 4.3) to determine if low citrate concentrations adversely effected the following: prothrombin time (PT), thrombin time (TT), Kaolin-cephalin clotting time (KCCT), ethanol gel (EG) and fibrinogen levels. Low citrate concentrations had no significant effect (Student’s t test for paired scores) on any clotting indice tested (see table below).

scholarly journals  The effect of various forms of selenium supplied to pregnant goats on selected blood parameters and on the concentration of Se in urine and blood of kids at the time of weaning

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 394-403 ◽  
Author(s):  
A. Pechova ◽  
L. Sevcikova ◽  
L. Pavlata ◽  
R. Dvorak
Keyword(s):  
Protein Complex ◽  
Total Antioxidant Status ◽  
Blood Parameters ◽  
Selenium Supplementation ◽  
The Other ◽  
Muscle Enzymes ◽  
Total Antioxidant ◽  
Negative Effect ◽  
Se Deficiency ◽  
Urine Selenium

&nbsp; The aim of this trial was to compare the effect of supplementation of goats with different forms of selenium on the metabolism of their kids at the time of weaning. The experiment was performed with 45 kids of mothers supplemented with various forms of selenium. Group C was control while the other four groups were supplemented with selenium for six weeks before (0.3 mg/goat/day) and after parturition (0.9 mg/goat/day). Group&nbsp;Se-I received sodium selenite while the other groups received organic forms: Se-lactate-protein complex (Se-L), Se-proteinate (Se-P) and Se-yeast (Se-Y). The kids were weaned at three months of age and samples of blood and urine were taken. Parameters monitored in the blood included Se, glutathione peroxidase (GPx), Zn, Cu, thyroxine, triiodothyronine, protein, immunoglobulins, muscle enzymes, total antioxidant status, vitamin A and E. Se levels were determined In the urine. Selenium supplementation of goats from six weeks before delivery significantly influenced selenium concentrations in the blood of kids. Significant differences (P &lt; 0.0002) were found between the control and all experimental groups and further between Se-Y and the other experimental groups (Se-Y: 243.0&nbsp;&plusmn; 20.3 &mu;g/l; Se-I: 156.3 &plusmn; 34.3 &mu;g/l; Se-P: 152.6 &plusmn; 41.5 &mu;g/l; Se-L: 146.7 &plusmn; 20 0 &mu;g/l; C: 67.6 &plusmn; 13.1 &mu;g/l). The highest concentration was found in the group supplemented with Se-yeast with a high content of selenomethionine. The other two organic forms of selenium (proteinate and lactate-protein complex) increased the concentration of Se in blood and the activity of GPx to the same extent as the inorganic form of selenium. Se supplementation did not have a negative effect on the concentration of copper and zinc in the blood serum of kids, but we found decreased concentrations of thyroxine in the experimental groups (Se-Y: 79.8 &plusmn; 12.8 nmol/l; Se-I: 66.5 &plusmn; 13.2 nmol/l; Se-P: 76.2 &plusmn; 25.7 nmol/l; Se-L: 84.5 &plusmn; 14.8 nmol/l; C: 92.7 &plusmn; 13.4 nmol/l). Significant differences were found between the group C and groups Se-I and Se-P (P &lt; 0.05). The supplementation of mothers with Se both in organic and inorganic forms was sufficient to prevent Se deficiency in kids at the time of weaning.

scholarly journals Influence of Psychological Factors in Federated Futsal and Lifeguard Athletes, Differences by Gender and Category

2021 ◽  
Vol 12 ◽  
Author(s):  
Francisco Cano-Noguera ◽  
Ricardo José Ibáñez-Pérez ◽  
Francisco Cavas-García ◽  
Alfonso Martínez-Moreno
Keyword(s):  
Statistical Tests ◽  
Competitive Anxiety ◽  
Utrecht Work Engagement Scale ◽  
Resilience Scale ◽  
Negative Effect ◽  
Kolmogorov Smirnov ◽  
And Gender ◽  
Student’S T ◽  
Positive Effect ◽  
Student’S T Test

This research aims to analyse the differences in optimism, resilience, engagement and competitive anxiety as a function of the sport modality practiced in lifeguarding (individual sport) and futsal (team sport); the sport category by age (cadet or youth) and gender. The LOT-R optimism questionnaire, the Connor-Davidson Resilience Scale (CD-RISC-10), the Utrecht Work Engagement Scale (UWES) and the Competitive Anxiety Scale (SAS-2) were applied to a sample of 189 participants (139 men and 50 womwn) aged between 14 and 17 years. The following statistical tests are performed: Cronbach's alpha, Pearson's linear correlation, Student's t-test, Kolmogorov-Smirnov test, Levene's test and multivariate linear regression. The data indicate that there are significant gender differences in total anxiety (p &lt;0.001) and all its dimensions (somatic, worry, worry-free), also in optimism and pessimism (p &lt; 0.001), as well as in total engagement (p = 0.051) and the absorption dimension (p &lt; 0.001). When comparing the sample by sport categories, there are statistically significant differences in somatic anxiety (p = 0.036) and deconcentration (p = 0.034), as well as in LOT-total (p ≤ 0.001) and pessimism (p ≤ 0.001). In relation to the sport modalities, lifeguards show more anxiety 38.39 (0.49) and more commitment 4.58 (0.87) while futsal athletes reach higher scores in deconcentration 8.45 (2.29). It is concluded that the variables of commitment and resilience had a statistically significant positive effect, and the category of &lt;16 years had a statistically significant negative effect, so the lower the category, the higher the optimism.

Serosal and cutaneous recordings of gastric myoelectrical activity in patients with gastroparesis

1994 ◽  
Vol 266 (1) ◽  
pp. G90-G98 ◽  
Author(s):  
J. D. Chen ◽  
B. D. Schirmer ◽  
R. W. McCallum
Keyword(s):  
Electrical Stimulation ◽  
Slow Wave ◽  
Test Meal ◽  
Frequency Change ◽  
Fasting State ◽  
Gastric Myoelectrical Activity ◽  
Myoelectrical Activity ◽  
Gastric Slow Wave ◽  
Student’S T ◽  
Student’S T Test

The aims of this study were to 1) investigate gastric myoelectrical activity in patients with gastroparesis, 2) validate the cutaneous electrogastrogram (EGG) in tracking the frequency change of the gastric slow wave, and 3) investigate the effect of electrical stimulation on gastric myoelectrical activity. Gastric myoelectrical activity was recorded in 12 patients with documented gastroparesis using serosal electrodes for > 200 min in each subject. All recordings were made at least 4 days after surgery. Each session consisted of a 30-min recording in the fasting state and a 30-min recording after a test meal. The test meal (liquid or mixed) was selected according to patient's tolerance. Electrical stimulation was performed in three subjects via the serosal electrodes at a frequency of 3 cycles/min. Gastric myoelectrical activity was recorded using serosal electrodes in each session. The serosal recording showed slow waves of 2.5 to 4.0 cycles/min in all 12 subjects. Absence of spikes was noted in 11 of the 12 subjects. The simultaneous serosal and cutaneous recording of gastric myoelectrical activity showed that the frequency of the EGG was exactly the same as that of the serosal recording. Liquid meals resulted in a significant decrease in slow-wave frequency (Student's t test, P = 0.006), and the EGG accurately reflected this change. Electrical stimulation had no effect on the frequency of the gastric slow wave and did not induce spikes.(ABSTRACT TRUNCATED AT 250 WORDS)

The Effect of Changes in Dialysate Volume on Glucose and Urea Equilibration

1994 ◽  
Vol 14 (3) ◽  
pp. 236-239 ◽  
Author(s):  
Edward C. Kohaut ◽  
F. Bryson Waldo ◽  
Mark R. Benfield
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Prospective Study ◽  
Body Surface ◽  
Patient Population ◽  
Different Ages ◽  
Student’S T ◽  
A Prospective Study ◽  
Dialysate Volume ◽  
Student’S T Test

Objectives To determine the effect of changing dialysate volume on urea and glucoseequilibration curves and to determine, if dialysate volume is prescribed on the basis of body surface area, whether equilibration curves will be consistent in patients of different sizes and ages. Design A prospective study wherein children with acute or chronic renal failure had peritoneal equilibration studies done with dwell volumes of 30 mL/kg, 40 mL/kg, and 1200 mL/m2. Patient Population Twenty-two children: 7 under 3 years of age; 8 between 3 and 10 years of age; 7 older than 10 years of age. Statistics Student's t-test. Results Urea and glucose equilibrated rapidly at dwell volumes of 30 mL/kg, slower at dwell volumes of 40 mL/kg, and slowest at dwell volumes of 1200 mL/m2. Equilibration curves were similar in children of different ages when dialysate volumes of 1200 mL/m2 were infused. Conclusion Dialysate volumes of 1200 mL/m2 should be used when equilibration studies are being done to compare individuals of different ages and sizes.

scholarly journals Development and Validation of 2-Azaspiro [4,5] Decan-3-One (Impurity A) in Gabapentin Determination Method Using qNMR Spectroscopy

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1656
Author(s):  
Nataliya E. Kuz’mina ◽  
Sergey V. Moiseev ◽  
Mikhail D. Khorolskiy ◽  
Anna I. Lutceva
Keyword(s):  
Test Procedure ◽  
Active Pharmaceutical Ingredient ◽  
Test Methods ◽  
Intermediate Precision ◽  
Coefficients Of Variation ◽  
Validation Parameters ◽  
Limit Of Quantitation ◽  
Student’S T ◽  
Development And Validation ◽  
Student’S T Test

The authors developed a 1H qNMR test procedure for identification and quantification of impurity A present in gabapentin active pharmaceutical ingredient (API) and gabapentin products. The validation studies helped to determine the limit of quantitation and assess linearity, accuracy, repeatability, intermediate precision, specificity, and robustness of the procedure. Spike-and-recovery assays were used to calculate standard deviations, coefficients of variation, confidence intervals, bias, Fisher’s F test, and Student’s t-test for assay results. The obtained statistical values satisfy the acceptance criteria for the validation parameters. The authors compared the results of impurity A quantification in gabapentin APIs and capsules by using the 1H qNMR and HPLC test methods.

scholarly journals Potential intravenous drug incompatibilities in a pediatric unit

2016 ◽  
Vol 14 (2) ◽  
pp. 185-189 ◽  
Author(s):  
Karla Dalliane Batista Leal ◽  
Ramon Weyler Duarte Leopoldino ◽  
Rand Randall Martins ◽  
Lourena Mafra Veríssimo
Keyword(s):  
Risk Factors ◽  
University Hospital ◽  
Intravenous Drug ◽  
Penicillin G ◽  
Cross Sectional ◽  
Relative Risks ◽  
Related Risk ◽  
Student’S T ◽  
Level Of Significance ◽  
Student’S T Test

ABSTRACT Objective To investigate potential intravenous drug incompatibilities and related risk factors in a pediatric unit. Methods A cross-sectional analytical study conducted in the pediatric unit of a university hospital in Brazil. Data on prescriptions given to children aged 0-15 years from June to October 2014 were collected. Prescriptions that did not include intravenous drugs and prescriptions with incomplete dosage regimen or written in poor handwriting were excluded. Associations between variables and the risk of potential incompatibility were investigated using the Student’s t test and ANOVA; the level of significance was set at 5% (p<0.05). Relative risks were calculated for each drug involved in potential incompatibility with 95% confidence interval. Results A total of 222 children participated in the study; 132 (59.5%) children were male and 118 (53.2%) were aged between 0 and 2 years. The mean length of stay was 7.7±2.3 days. Dipyrone, penicillin G and ceftriaxona were the most commonly prescribed drugs. At least one potential incompatibility was detected in about 85% of children (1.2 incompatibility/patient ratio). Most incompatibilities detected fell into the non-tested (93.4%), precipitation (5.5%), turbidity (0.7%) or chemical decomposition (0.4%) categories. The number of drugs and prescription of diazepam, phenytoin, phenobarbital or metronidazole were risk factors for potential incompatibility. Conclusion Most pediatric prescriptions involved potential incompatibilities, with higher prevalence of non-tested incompatibilities. The number of drugs and prescription of diazepam, phenobarbital, phenytoin or metronidazole were risk factors for potential incompatibilities.

scholarly journals Indicators of Targeted Physical Fitness in Judo and Jujutsu—Preliminary Results of Research

2021 ◽  
Vol 18 (8) ◽  
pp. 4347
Author(s):  
Wojciech J. Cynarski ◽  
Jan Słopecki ◽  
Bartosz Dziadek ◽  
Peter Böschen ◽  
Paweł Piepiora
Keyword(s):  
Reaction Time ◽  
Visual Stimulation ◽  
Intrinsic Value ◽  
Reaction Times ◽  
Arithmetic Means ◽  
Simple Reaction ◽  
Martial Art ◽  
Student’S T ◽  
Pearson Correlations ◽  
Student’S T Test

(1) Study aim: This is a comparative study for judo and jujutsu practitioners. It has an intrinsic value. The aim of this study was to showcase a comparison of practitioners of judo and a similar martial art jujutsu with regard to manual abilities. The study applied the measurement of simple reaction time in response to a visual stimulus and handgrip measurement. (2) Materials and Methods: The group comprising N = 69 black belts from Poland and Germany (including 30 from judo and 39 from jujutsu) applied two trials: “grasping of Ditrich rod” and dynamometric handgrip measurement. The analysis of the results involved the calculations of arithmetic means, standard deviations, and Pearson correlations. Analysis of the differences (Mann–Whitney U test) and Student’s t-test were also applied to establish statistical differences. (3) Results: In the test involving handgrip measurement, the subjects from Poland (both those practicing judo and jujutsu) gained better results compared to their German counterparts. In the test involving grasping of Ditrich rod, a positive correlation was demonstrated in the group of German judokas between the age and reaction time of the subjects (rxy = 0.66, p < 0.05), as well as in the group of jujutsu subjects between body weight and the reaction time (rxy = 0.49, p < 0.05). A significant and strong correlation between handgrip and weight was also established for the group of German judokas (rxy = 0.75, p < 0.05). In Polish competitors, the correlations were only established between the age and handgrip measurements (rxy = 0.49, p < 0.05). (4) Conclusions: Simple reaction times in response to visual stimulation were shorter in the subjects practicing the martial art jujutsu. However, the statement regarding the advantage of the judokas in terms of handgrip force was not confirmed by the results.

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