scholarly journals Antimicrobial Phenolic Compounds from Anabasis Aphylla L

2009 ◽  
Vol 4 (3) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Hua Du ◽  
Ye Wang ◽  
Xiaojiang Hao ◽  
Chun Li ◽  
Youliang Peng ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Benzoic Acid ◽  
Cinnamic Acid ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Control Plant ◽  
Acid Methyl Ester ◽  
Spectrometric Analysis ◽  
Bacterium Bacillus

Bioassay-guided fractionation of an ethyl acetate extract from the aerial parts of Anabasis aphylla, a Chenopodiaceous species widely distributed in the northwest of China, led to the isolation of six phenolic compounds, which were identified by means of spectrometric analysis as 1-(2-hydroxy-4,6-dimethoxyphenyl)-ethanone (1), 3,4-dihydroxy cinnamic acid tetracosyl ester (2), 4-hydroxy-3-methoxy benzoic acid (3), 2-hydroxy benzoic acid (4), 3,4-dihydroxy cinnamic acid methyl ester (5) and 4-hydroxy benzoic acid pentadecane ester (6). These compounds were further screened for their minimum inhibitory concentration (MIC) and median inhibitory concentration (IC50) by use of the microdilution-MTT assay for antimicrobial activity against one Gram-positive bacterium ( Bacillus subtilis), three Gram-negative bacteria ( Agrobacterium tumefaciens, Pseudomonas lachrymans, and Xanthomonas vesicatoria), and one yeast ( Candida albicans). Apart from compound 6, which had no activity against any of the tested microorganisms, the other compounds showed selective inhibitory activity. This is the first report on the antimicrobial activity of the phenolic compounds isolated from A. aphylla. The obtained results provide promising baseline information for the potential use of the extract and some isolated compounds from this plant as antimicrobial agents to control plant and animal diseases.

scholarly journals Antimicrobial Activity of Leaves and Flowers of Cassia auriculata linn

1970 ◽  
Vol 46 (4) ◽  
pp. 513-518 ◽  
Author(s):  
V Subhadradevi ◽  
K Asokkumar ◽  
M Umamaheswari ◽  
AT Sivashanmugam ◽  
JR Ushanandhini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Dominant Role ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Cassia Auriculata ◽  
Potential Source ◽  
Wide Range ◽  
Ancient Times

Since ancient times plant as sources of medicinal compounds have continued to play a dominant role in the maintenance of human health. To treat chronic and infectious diseases plants used in traditional medicine contain a wide range of ingredients. In this regard, Cassia auriculata L. (Caesalpiniaceae) is widely used in Ayurvedic medicine as a tonic, astringent and as a remedy for diabetes, conjunctivitis, ulcers, leprosy, skin and liver diseases. The aim of present study was to evaluate the antimicrobial activity of ethanolic extract of Cassia auriculata leaves and flowers (CALE & CAFE). CALE and CAFE exhibited broad spectrum antimicrobial activity against standard strains of Staphylococcus aureus, Escherichia coli and Bacillus subtilis and exhibited no antifungal activity against standard strains of Candida albicans and Aspergillus niger. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) was carried out for CALE and CAFE. The results obtained in the present study indicate that the CALE and CAFE can be a potential source of natural antimicrobial agents. Key words: Cassia auriculata; Antimicrobial activity; Agar well diffusion method. DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9600 BJSIR 2011; 46(4): 513-518

Antioxidant Activity and Phenolic Compound Profile of Pistachio Skins (Pistacia vera L., Cultivars Kallehghuchi and Ohadi)

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Shima Azadedel ◽  
Parichehr Hanachi ◽  
Azra Saboora
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Benzoic Acid ◽  
Gallic Acid ◽  
Cinnamic Acid ◽  
High Mass ◽  
Total Phenolic ◽  
Pistacia Vera ◽  
Native Plant ◽  
Pharmaceutical Industries

Background: Pistachio, with the scientific name Pistacia vera L., a native plant in Asia, is a member of the Anacardiaceae family. Pistachio nuts and skins are known as a rich source of phenolic compounds with antioxidant, anti-inflammatory, and antimicrobial properties. Objectives: In the current study, the industrial production of phenolic compounds with antioxidant activity was investigated because of the high mass production of pistachio skin in Iran. Methods: The extraction of two pistachio cultivars, namely Kalleghuchi and Ohadi were carried out by using two methods (maceration and ultrasonic extraction) and four solvents (acetone 70%, ethanol 50%, methanol 50%, and water). Antioxidant properties of pistachio skins were determined by three methods (Folin-Ciocalteau colorimetric method, DPPH assay, TLC/DPPH analysis). Results: The results showed that the highest content of total phenolic compounds were measured by ultrasonic and maceration methods related to Ohadi and Kallehghuchi in acetone solvents 17.4 ± 0.04 and 17.26 ± 0.1 mg/g DW, respectively. The highest antioxidant activity was measured by ultrasonic and maceration methods related to Ohadi in acetone and water solvents IC50 = 0.057 ± 0.001 and 0.059 ± 0.002 µg/ml, respectively. By TLC/DPPH analysis, gallic acid, 4-hydroxy-3, 5-dimethoxy benzoic acid, tannic acid, and some unidentified compounds were determined. By HPLC analysis, gallic acid, coumaric acid, cinnamic acid, 4-hydroxy-cinnamic acid, and 4-hydroxy benzoic acid were determined. Conclusions: In conclusion, this study clarifies some special biochemical characteristics of pistachio skins. Therefore, according to the results of the study, pistachio skins could be successfully used in the food and pharmaceutical industries.

scholarly journals Manganese (II) and Cobalt (II) Acetylacetonates as Antimicrobial Agents

2019 ◽  
Vol 1 ◽  
pp. 176-185
Author(s):  
S G Yiase ◽  
S O Adejo ◽  
S T Iningev
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Dilution Method ◽  
Spectroscopic Techniques ◽  
Metal Chlorides ◽  
Activity Screening ◽  
Antibacterial And Antifungal ◽  
Broth Dilution

Mn(II) and Co(II) complexes were prepared by reaction of the metal chlorides with acetylacetone in ammonical aqueous medium. The metal complexes were prepared in order to investigate their antimicrobial activity on some selected pathogens. The characterisation of the complexes was on the basis of various spectroscopic techniques like infrared and ultraviolet studies. The compounds were subjected to antimicrobial activity screening using serial broth dilution method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) were determined. Mn(II) complex has shown significantly both antibacterial and antifungal activity with a MIC of 1.25 μg/mL while Co(II) complex was noticeable for antifungal activity at the same concentration. Whereas Mn(II) acetylacetonate is a more potent bactericide while Co(II) acetylacetonate is a more potent fungicide, both with MBC/MFC value of 2.5 μg/mL. Antimicrobial agent of the ligand has enhanced on complexation with Mn(II) and Co(II) ions. Though, the potency of the prepared antibiotics on the tested microbes is less compared to the standard drugs (Ciprofloxacin and Fulcin).

scholarly journals The In Vitro Antimicrobial Effects of Lavandula angustifolia Essential Oil in Combination with Conventional Antimicrobial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Stephanie de Rapper ◽  
Alvaro Viljoen ◽  
Sandy van Vuuren
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Lavandula Angustifolia ◽  
Gram Negative ◽  
Stock Solutions

The paper focuses on the in vitro antimicrobial activity of Lavandula angustifolia Mill. (lavender) essential oil in combination with four commercial antimicrobial agents. Stock solutions of chloramphenicol, ciprofloxacin, nystatin, and fusidic acid were tested in combination with L. angustifolia essential oil. The antimicrobial activities of the combinations were investigated against the Gram-positive bacterial strain Staphylococcus aureus (ATCC 6538) and Gram-negative Pseudomonas aeruginosa (ATCC 27858) and Candida albicans (ATCC 10231) was selected to represent the yeasts. The antimicrobial effect was performed using the minimum inhibitory concentration (MIC) microdilution assay. Isobolograms were constructed for varying ratios. The most prominent interaction was noted when L. angustifolia essential oil was combined with chloramphenicol and tested against the pathogen P. aeruginosa (ΣFIC of 0.29). Lavendula angustifolia essential oil was shown in most cases to interact synergistically with conventional antimicrobials when combined in ratios where higher volumes of L. angustifolia essential oil were incorporated into the combination.

Combination Effects of the Antimicrobial Agents and Cinnamon Oil

2012 ◽  
Vol 506 ◽  
pp. 246-249 ◽  
Author(s):  
J. Mahadlek ◽  
Juree Charoenteeraboon ◽  
T. Phaechamud
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Combination Effect ◽  
Additive Effects ◽  
Fractional Inhibitory Concentration ◽  
Doxycycline Hyclate ◽  
Cinnamon Oil ◽  
Cinnamon Essential Oil

The use of antimicrobial combination may be interesting because it can decrease doses and side effects of antimicrobial agents. The cinnamon essential oil was examined with respect to antimicrobial modifying activity. The combination effect of antimicrobial agents (doxycycline hyclate, ciprofloxacin HCl and metronidazole) and cinnamon oil was evaluated by checkerboard method to obtain a fractional inhibitory concentration (FIC) index. The effect of metronidazole+doxycycline hyclate and metronidazole+ciprofloxacin hydrochloride combinations were additive against Staphylococcus aureus. The indifference FIC index was observed only with the doxycycline hyclate+ciprofloxacin HCl combination. Combinations of cinnamon oil and all antimicrobial agents showed the additive effects against S. aureus. These results indicated that the antimicrobial activity of cinnamon oil was generally additive to all test antimicrobial agents.

scholarly journals Novel 2,4,6-Trimethylbenzenesulfonyl Hydrazones with Antibacterial Activity: Synthesis and In Vitro Study

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2723
Author(s):  
Łukasz Popiołek ◽  
Sylwia Szeremeta ◽  
Anna Biernasiuk ◽  
Monika Wujec
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Potential Application ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
In Vitro Study ◽  
In Vitro Bioactivity ◽  
Bacterial Strains

This research describes the synthesis and in vitro antimicrobial activity study of a series of 2,4,6-trimethylbenzenesulfonyl hydrazones. Twenty-five hydrazones (2–26) were synthesized on the basis of condensation reaction. The in vitro bioactivity study confirmed the potential application of obtained derivatives as antimicrobial agents. Among the tested compounds, the highest activity was discovered for derivative 24, which possessed minimal inhibitory concentration (MIC) ranging from 7.81 to 15.62 µg/mL against Gram-positive reference bacterial strains. Synthesized benzenesulfonyl hydrazones can be applied as potential ligands for the synthesis of bioactive metal complexes.

scholarly journals Antimicrobial Compounds from the Endophytic Fungus Fusarium sp. Ppf4 Isolated from the Medicinal Plant Paris polyphylla var. yunnanensis

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Yongfu Huang ◽  
Jianglin Zhao ◽  
Ligang Zhou ◽  
Mingan Wang ◽  
Jingguo Wang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungus ◽  
Control Plant ◽  
Fungal Spore ◽  
Spectrometric Analysis ◽  
Medicinal Species ◽  
Paris Polyphylla ◽  
Bioassay Guided Fractionation ◽  
Baseline Information ◽  
Fusarium Sp

Two sterols and one fatty acid were obtained by bioassay-guided fractionation from the light petroleum extract of the fungus Fusarium sp. Ppf4 isolated from the rhizomes of Paris polyphylla var. yunnanensis Hand.-Mazz., a medicinal species distributed in the southwest of China. The compounds were elucidated as 5α, 8α-epidioxyergosta-6, 22-dien-3β-ol (1), ergosta-8(9), 22-dien-3β, 5α, 6β, 7α-tetraol (2), and butanedioic acid (3), respectively, by means of physical and spectrometric analysis. Both fungal spore germination and microdilution-MTT assays were employed to evaluate their antimicrobial activity. Compound 1 was found to be the most bioactive, and compound 3 less active against the test pathogens. This is the first report of the antimicrobial activity of the compounds isolated from the endophytic Fusarium sp. Ppf4 associated with P. polyphylla var. yunnanensis. The results provide promising baseline information for the potential use of the compounds of this endophytic fungus as an antimicrobial agent to control plant and animal diseases.

scholarly journals SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF SOME NOVEL 3,4-DISUBSTITUTED PYRAZOLE DERIVATIVES

2019 ◽  
pp. 129-133
Author(s):  
GANESH AKULA ◽  
KAUSHAL K CHANDRUL ◽  
BHIKSHAPATHI DVRN
Keyword(s):  
Antimicrobial Activity ◽  
Hydrazine Hydrate ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Acid Methyl Ester ◽  
Dimethyl Acetal ◽  
Inhibitory Effect ◽  
Dimethyl Formamide ◽  
Highly Sensitive ◽  
Substituted Pyrazoles

Objective: The objective of the current work was to synthesize a series of 3,4-substituted pyrazoles from the cyclization of substituted aryl ethanone and hydrazine hydrate in a two-step process and screen the derivatives for their antimicrobial activity. Methods: The title compounds were derived from the condensation of ethanone intermediate with N, N-Dimethyl formamide-dimethyl acetal and hydrazine hydrate. Ethanone intermediate synthesized from substituted methyl phenylacetate in the presence of potassium t-butoxide with 6-methyl pyridine-2-carboxylic acid methyl ester. Results: The final products were characterized by detailed spectral analysis using Mass, Nuclear Magnetic Resonance, and Infra Red spectroscopy. All the compounds (4a-4j) showed significant antibacterial properties on both Gram-positive and Gram-negative bacteria. Interestingly, the selected microbes were found to be highly sensitive for compound 4a, 4c, 4d, 4h, and 4i. The molecules are also antifungal in nature, and they have a significant inhibitory effect on the growth of Candida albicans and Aspergillus niger. Conclusion: The results suggest that the developed derivatives bearing the pyrazole nucleus could be the lead structures for the development of antimicrobial agents for fatal infections.

scholarly journals Antimicrobial activity and bioautographic study of antistaphylococcal components from Caesalpinia pyramidalis Tull.

2012 ◽  
Vol 48 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Antonio Marcos Saraiva ◽  
Cristiane Lopes Saraiva ◽  
Admário Marques Gonçalves ◽  
Rogério Ribeiro Soares ◽  
Fabrício de Oliveira Mendes ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Phytochemical Study ◽  
Multiresistant Strains ◽  
Active Substances ◽  
Ethyl Acetate Extracts

The antimicrobial activity of dry methanol and ethyl acetate extracts for the leaves, bark of the stem, peel of the root, flower, fruit and seed of Caesalpinia pyramidalis Tull. (catingueira) was performed against seventeen isolates of Staphylococcus aureus MRSA multiresistant strains, which included two isolates of S. aureus MSSA and two ATCC strains. The antimicrobial activity was tested by the agar diffusion method and the Minimum Inhibitory Concentration (MIC) was determined. The dry methanol extract of the root showed good antimicrobial activity with a MIC of less than 0.5 mg.mL-1. The dry ethyl acetate extracts exhibited lower antimicrobial activity, which might be explained by solubility problems and less diffusion in the agar medium. Results of the bioautographies also confirmed inhibition halos corresponding to the active substances present in the leaves, as well as in the flower of C. pyramidalis. The phytochemical study of the leaves, bark of the stem, peel of the root, flower and fruit of extracts from C. pyramidalis confirmed the presence of a number of known antimicrobial agents including ursolic acid, quercetin, catechin, ellagic acid, sitosterol, flavonoids, proanthocyanidins and gallic acid.

scholarly journals Determination of Phenolic Compounds with Antimicrobial Activity of Byrsonima Crassifolia and Inga Edulis Leaves Extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 21-28
Author(s):  
Victor Hugo Alves do Nascimento ◽  
Alessandra Carla Guimarães Sobrinho ◽  
Cintya De Oliveira Souza ◽  
Jesus Nazareno Silva de Souza ◽  
Consuelo Lúcia Sousa
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Minimum Inhibitory Concentration ◽  
Gallic Acid ◽  
Inhibitory Concentration ◽  
Disk Diffusion ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Inga Edulis ◽  
Byrsonima Crassifolia

The aim of this work was to determine the compounds with antimicrobial capacity from crude extracts and leaf fractions of Byrsonima crassifolia and Inga edulis. A solid-liquid extraction was performed using three solvent systems (acetate, methanol and methanol: water). The extracts and fractions were submitted to antimicrobial susceptibility tests for Gram-negative and Gram-positive bacteria by disk diffusion and Minimum Inhibitory Concentration (MIC) methods. A solid phase extraction (SPE) was performed to identify phenolic compounds for pre-concentration and pre-purification of the extracts and fractions that presented antimicrobial potential. The methanolic fraction was able to extract the highest total phenolic content and also presented the largest halos in the disk diffusion test. The minimum inhibitory concentration ranged from 125 to 500 μg/ml mL-1 and from 12.5 to 200 μg/ml mL-1 for Gram-negative and Gram-positive bacteria, respectively. After SPE, the crude extracts and fractions showed inhibition halos against all themicroorganisms tested at a lower concentration when compared to the standardized antibiotics. Gallic acid, ferric acid, myricetin and quercetin showed antimicrobial activity when tested alone. Gallic acid, catechin, epicatechin, ferrulic acid, quercetin, and other 4 major compounds were identified and quantified for Byrsonima crassifolia and 3 compounds for Inga edulis.   Keywords: Vegetable Matrices. Antibiotics. Bioactive Compounds. Liquid Chromatography.   Resumo O objetivo do presente estudo foi determinar compostos com capacidade antimicrobiana de extratos brutos e frações de folhas de Byrsonima crassifolia e de Inga edulis. Foi realizado uma extração sólido-líquido utilizando três sistemas de solventes (acetato, metanol e metanol:água). Os extratos e frações foram submetidos aos testes de suscetibilidade antimicrobiana para bactérias Gram-negativas e Gram-positivas através dos métodos de disco-difusão e Concentração Inibitória Mínima-CIM. Para identificação dos compostos fenólicos foi realizada Extração em Fase Sólida (SPE), para pré- concentração e pré-purificação dos extratos e frações que apresentaram potencial antimicrobiano. A fração metanólica conseguiu extrair maior conteúdo de fenólicos totais, e apresentou os maiores halos no teste de disco-difusão. A concentração inibitória mínima variou de 125 a 500 µgES mL-1   e de 12,5 a 200 µgES mL-1 para bactérias Gram-negativas e Gram-positivas, respectivamente. Após SPE, os extratos brutos e frações apresentaram halos de inibição frente a todos os micro-organismos testados em uma concentração menor que a dos antibióticos padronizados. O ácido gálico, ácido ferrúlico, miricetina e quercetina apresentaram atividade antimicrobiana quando testados isoladamente. Foram identificados e quantificados nos extratos brutos e frações ácido gálico, catequina, epicatequina, ácido ferrúlico, quercetina, além de outros 4 compostos majoritários para Byrsonima crassifolia e 3 compostos para Inga edulis.   Palavras-chave: Matrizes Vegetais. Antibióticos. Compostos Bioativos. Cromatografia Líquida.

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