scholarly journals Use of zebrafish larvae lateral line to study protection against cisplatin-induced ototoxicity: A scoping review

2020 ◽  
Vol 34 ◽  
pp. 205873842095955
Author(s):  
Ewa Domarecka ◽  
Magda Skarzynska ◽  
Agnieszka J Szczepek ◽  
Stavros Hatzopoulos
Keyword(s):  
Short Exposure ◽  
Sensory Cells ◽  
High Concentration ◽  
Larval Zebrafish ◽  
Zebrafish Larvae ◽  
Mesh Terms ◽  
Hearing Thresholds ◽  
Experimental Protocols ◽  
Fish Hearing ◽  
Inner Ear Damage

Aim: The present review aimed to consolidate and analyze the recent information about the use of zebrafish in studies concerning cisplatin-induced ototoxicity and otoprotection. Material and methods: The PubMed, Web of Science, and Scopus databanks were searched using the following MESH terms: zebrafish, cisplatin, ototoxicity. The identified publications were screened according to inclusion and exclusion criteria and the 26 qualifying manuscripts were included in the full-text analysis. The experimental protocols, including cisplatin concentrations, the exposure duration and the outcome measurements used in zebrafish larvae studies, were evaluated and the reported knowledge was summarized. Results: Twenty-six substances protecting from cisplatin-induced toxicity were identified with the use of zebrafish larvae. These substances include quinine, salvianolic acid B, berbamine 6, benzamil, quercetin, dexmedetomidine, dexamethsanone, quinoxaline, edaravone, apocynin, dimethyl sulfoxide, KR-22335, SRT1720, ORC-13661, 3-MA, D-methionine, mdivi-1, FUT-175, rapamycin, Z-LLF-CHO, ATX, NAC, CYM-5478, CHCP1, CHCP2 and leupeptin. The otoprotective effects of compounds were attributed to their anti-ROS, anti-apoptotic and cisplatin uptake-blocking properties. The broadest range of protection was achieved when the experimental flow used preconditioning with an otoprotective compound and later a co-incubation with cisplatin. Protection against a high concentration of cisplatin was observed only in protocols using short exposure times (4 and 6 h). Conclusions: The data extracted from the selected papers confirm that despite the differences between the human and the zebra fish hearing thresholds (as affected by cisplatin), the sensory cells of zebrafish and larval zebrafish are a valuable tool which could be used: (i) for the discovery of novel otoprotective substances and compounds; (ii) to screen their side effects and (iii) to extend the knowledge on the mechanisms of cisplatin-induced inner ear damage. For future studies, the development of a consensus experimental protocol is highly recommended.

scholarly journals BonZeb: open-source, modular software tools for high-resolution zebrafish tracking and analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicholas C. Guilbeault ◽  
Jordan Guerguiev ◽  
Michael Martin ◽  
Isabelle Tate ◽  
Tod R. Thiele
Keyword(s):  
High Resolution ◽  
Open Loop ◽  
Ease Of Use ◽  
Neural Mechanisms ◽  
Animal Tracking ◽  
Larval Zebrafish ◽  
Optogenetic Stimulation ◽  
Modular Software ◽  
Behavioral Tracking ◽  
Experimental Protocols

AbstractWe present BonZeb—a suite of modular Bonsai packages which allow high-resolution zebrafish tracking with dynamic visual feedback. Bonsai is an increasingly popular software platform that is accelerating the standardization of experimental protocols within the neurosciences due to its speed, flexibility, and minimal programming overhead. BonZeb can be implemented into novel and existing Bonsai workflows for online behavioral tracking and offline tracking with batch processing. We demonstrate that BonZeb can run a variety of experimental configurations used for gaining insights into the neural mechanisms of zebrafish behavior. BonZeb supports head-fixed closed-loop and free-swimming virtual open-loop assays as well as multi-animal tracking, optogenetic stimulation, and calcium imaging during behavior. The combined performance, ease of use and versatility of BonZeb opens new experimental avenues for researchers seeking high-resolution behavioral tracking of larval zebrafish.

Motility and energy-rich phosphorus compounds in spermatozoa of Octopus dofleini martini

1971 ◽  
Vol 178 (1051) ◽  
pp. 151-160 ◽  
Keyword(s):  
Sea Water ◽  
Phosphorus Compounds ◽  
Short Exposure ◽  
Motile Sperm ◽  
High Concentration ◽  
Irreversible Loss ◽  
Prolonged Incubation ◽  
The North ◽  
Sperm Suspension ◽  
The North Pacific

The spermatozoa of the giant octopus of the North Pacific, freshly removed from spermatophores, showed very little motility, but on dilution with sea-water or 2.7 % NaCl, followed by dialysis against either of these two media, they became intensely motile and remained so for several days at 2 to 10 °C. At higher temperatures, particularly above 25 °C, octopus spermatozoa lost their motility rapidly. At 35 °C, complete and irreversible loss of motility occurred within less than 1 min. The motility of octopus spermatozoa at 2 to 10 °C persisted under both anaerobic and aerobic conditions and did not require the presence of exogenous glycolysable sugar. The addition of spermatophoric plasma to a motile sperm suspension inhibited motility. Other inhibitors were sodium azide, 2, 4-dinitrophenol and ethylenediaminetetra-acetate, at 0.001 M concentrations. ATP, ADP and arginine phosphate have been identified and quantitatively measured in octopus spermatozoa. On prolonged incubation of motile sperm suspensions a t 3 °C, ATP and ADP did not decline appreciably, whilst arginine phosphate decreased considerably. The decrease was even more pronounced in sperm suspensions which had first been inactivated by short exposure to 35 °C, prior to prolonged incubation at 3 °C. Glycogen, the main carbohydrate store of octopus spermatozoa, remained at a high concentration even in sperm suspensions kept for 5 days at 3 °C, and there was no appreciable difference in that respect between a sample containing motile spermatozoa and one in which, at the outset of incubation, the spermatozoa were immobilized by heating to 35 °C.

scholarly journals Fumonisin B1 Production by Fusarium Species and Mycotoxigenic Effect on Larval Zebrafish

2020 ◽  
Vol 31 (3) ◽  
pp. 91-107 ◽  
Author(s):  
Najihah Azman ◽  
Nur Ain Izzati Mohd Zainudin ◽  
Wan Norhamidah Wan Ibrahim
Keyword(s):  
Liquid Chromatography ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Fusarium Species ◽  
Fumonisin B1 ◽  
Larval Zebrafish ◽  
Zebrafish Larvae ◽  
Significant Difference ◽  
Successful Amplification ◽  
Fast Liquid Chromatography

Fumonisin B1 (FB1) is a common mycotoxin produced by Fusarium species particularly F. proliferatum and F. verticillioides. The toxin produced can cause adverse effects on humans and animals. The objectives of this study were to detect the production of FB1 based on the amplification of FUM1 gene, to quantify FB1 produced by the isolates using Ultra-fast Liquid Chromatography (UFLC) analysis, to examine the embryotoxicity effect of FB1 and to determine EC50 toward the larvae of zebrafish (Danio rerio). Fifty isolates of Fusarium species were isolated from different hosts throughout Malaysia. Successful amplification of the FUM1 gene showed the presence of this gene (800 bp) in the genome of 48 out of 50 isolates. The highest level of FB1 produced by F. proliferatum isolate B2433 was 6677.32 ppm meanwhile F. verticillioides isolate J1363 was 954.01 ppm. From the assessment of embryotoxicity test of FB1 on larvae of zebrafish, five concentrations of FB1 (0.43 ppm, 0.58 ppm, 0.72 ppm, 0.87 ppm and 1.00 ppm) were tested. Morphological changes of the FB1 exposed-larvae were observed at 24 to 168 hpf. The mortality rate and abnormality of zebrafish larvae were significantly increased at 144 hpf exposure. Meanwhile, the spontaneous tail coiling showed a significant difference. There were no significant differences in the heartbeat rate. As a conclusion, the presence of FUM1 in every isolate can be detected by FUM1 gene analysis and both of the species produced different concentrations of FB1. This is the first report of FB1 produced by Fusarium species gave a significant effect on zebrafish development.

scholarly journals Osmotic surveillance mediates rapid wound closure through nucleotide release

2014 ◽  
Vol 207 (6) ◽  
pp. 767-782 ◽  
Author(s):  
William J. Gault ◽  
Balázs Enyedi ◽  
Philipp Niethammer
Keyword(s):  
Wound Repair ◽  
Interstitial Fluid ◽  
Wound Closure ◽  
External Environment ◽  
Atp Release ◽  
Nucleoside Triphosphate ◽  
Extracellular Nucleotide ◽  
Nucleoside Triphosphate Diphosphohydrolase ◽  
Larval Zebrafish ◽  
Zebrafish Larvae

Osmotic cues from the environment mediate rapid detection of epithelial breaches by leukocytes in larval zebrafish tail fins. Using intravital luminescence and fluorescence microscopy, we now show that osmolarity differences between the interstitial fluid and the external environment trigger ATP release at tail fin wounds to initiate rapid wound closure through long-range activation of basal epithelial cell motility. Extracellular nucleotide breakdown, at least in part mediated by ecto-nucleoside triphosphate diphosphohydrolase 3 (Entpd3), restricts the range and duration of osmotically induced cell migration after injury. Thus, in zebrafish larvae, wound repair is driven by an autoregulatory circuit that generates pro-migratory tissue signals as a function of environmental exposure of the inside of the tissue.

scholarly journals Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)

2021 ◽  
Vol 322 ◽  
pp. 02016
Author(s):  
Uthpala Padeniya ◽  
Shafira Septriani ◽  
Arjay Pataueg ◽  
Christopher L. Brown
Keyword(s):  
Growth And Development ◽  
Digestive System ◽  
Survival Rates ◽  
Gi Tract ◽  
Larval Zebrafish ◽  
Zebrafish Larvae ◽  
Probiotic Treatment ◽  
Significant Difference ◽  
Digital Microscope ◽  
Control Survival

Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock culture tank as the control. Survival of the larvae throughout 10 dpf was recorded. To determine the development of the gastrointestinal tract DASPEI stain was used for larvae at the age of 3 dpf to 6dpf. The intensity of the fluorescence in each larva was observed through the automated digital microscope. According to the experiment results, a significant difference (P<0.0001) in the survival rates among all treatments was recorded. The probiotic-treated larvae (PTL) had a higher survival rate. This could be due to the presence of lactic acid bacteria in the probiotic treatment, which helps in enhancing immunity. In DASPEI staining, also PTL exhibits more fluorescence in the GI tract at 24 hours to 48 hours post-hatch than the other two treatments. The main possible reason behind this could also be the presence of Lactobacillus spp. Which directly influences the higher activity of the digestive system.

scholarly journals BonZeb: Open-source, modular software tools for high-resolution zebrafish tracking and analysis

2021 ◽  
Author(s):  
Nicholas C. Guilbeault ◽  
Jordan Guerguiev ◽  
Michael Martin ◽  
Isabelle Tate ◽  
Tod R. Thiele
Keyword(s):  
High Resolution ◽  
Open Loop ◽  
Ease Of Use ◽  
Neural Mechanisms ◽  
Animal Tracking ◽  
Larval Zebrafish ◽  
Optogenetic Stimulation ◽  
Modular Software ◽  
Behavioral Tracking ◽  
Experimental Protocols

AbstractWe present BonZeb – a suite of modular Bonsai packages which allow high-resolution zebrafish tracking with dynamic visual feedback. Bonsai is an increasingly popular software platform that is accelerating the standardization of experimental protocols within the neurosciences due to its speed, flexibility, and minimal programming overhead. BonZeb can be implemented into novel and existing Bonsai workflows for online behavioral tracking and offline tracking with batch processing. We demonstrate that BonZeb can run a variety of experimental configurations used for gaining insights into the neural mechanisms of zebrafish behavior. BonZeb supports head-fixed closed-loop and free-swimming virtual open-loop assays as well as multi-animal tracking, optogenetic stimulation, and calcium imaging during behavior. The combined performance, ease of use and versatility of BonZeb opens new experimental avenues for researchers seeking high-resolution behavioral tracking of larval zebrafish.

scholarly journals Developmental Toxicity and Neurotoxicity Assessment of R-, S-, and RS-Propylene Glycol Enantiomers in Zebrafish (Danio Rerio) Larvae

2021 ◽  
Author(s):  
Chao Shen ◽  
Xijing Zhao ◽  
Chengyong He ◽  
Zhenghong Zuo
Keyword(s):  
Propylene Glycol ◽  
Animal Feed ◽  
Developmental Toxicity ◽  
Oil Industry ◽  
Larval Zebrafish ◽  
Zebrafish Larvae ◽  
Chiral Carbon ◽  
Significant Difference ◽  
Eye Diameter ◽  
High Doses

Abstract Propylene glycol (PG) is widely used in the foods, pharmaceuticals, oil industry, animal feed, cosmetics and other industries. Because of the existence of a chiral carbon center, PG forms R (Rectus)- and S (Sinister)-enantiomers. Currently, the toxicity study of its R-, S-enantiomers is still very scarce. In this study, we have assessed the developmental toxicity and neurotoxicity of the R-, S-, and RS-PG enantiomers in zebrafish larvae. We found that exposure to R-, S-, and RS-PG enantiomers did not significantly affect the basic developmental endpoints of embryos or larvae (i.e., embryonic movement, hatching, mortality, malformation, heartbeat, body length), indicating that R-, S-, and RS-PG exposures did not exhibit the basic developmental toxicity in zebrafish larvae. The toxicity of the three enantiomers was lower than that of ethanol, and there was no significant difference between them. However, R-, S-, and RS-PG exposures with high doses could significantly change the eye diameter and the locomotor activity of larval zebrafish, indicating that R-, S-, and RS-PG enantiomers of high doses can potentially exhibit the neurotoxicity and ocular developmental toxicity in zebrafish larvae. Therefore, the potential neurotoxicity and ocular developmental toxicity of R-, S-, and RS-PG enantiomers for infants and toddlers should be considered.

scholarly journals Comparison the Effect of Ferutinin and 17β-Estradiol on Bone Mineralization of Developing Zebrafish (Danio rerio) Larvae

2019 ◽  
Vol 20 (6) ◽  
pp. 1507 ◽  
Author(s):  
Hoda Zare Mirakabad ◽  
Mohammad Farsi ◽  
Saeed Malekzadeh Shafaroudi ◽  
Abdolreza Bagheri ◽  
Mehrdad Iranshahi ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Bone Mineralization ◽  
Rt Pcr ◽  
High Concentration ◽  
Treatment Groups ◽  
Time Points ◽  
Zebrafish Larvae ◽  
Similar Expression Pattern ◽  
Novel Drugs ◽  
17Β Estradiol

There is an urgent need to develop novel drugs for osteoporosis which occurs due to estrogen deficiency. Phytoestrogens derived from medicinal plants would be the best alternative to chemical drugs with harmful side effects. The main purpose of the present study was to investigate the effect of ferutinin compared to 17β-estradiol (E2) on bone mineralization of zebrafish larvae. Regarding the lack of publications, the histology analysis was performed after exposure to E2 to find effective treatment on bone mineralization of developing zebrafish larvae. Then, the larvae were exposed to four concentrations of ferutinin at three time points to assess the mortality, the expression of some related genes and histology of the ceratohyal and hyomandibular of treated larvae. The RT-PCR result of the treatment groups demonstrated the similar expression pattern in the larvae which were exposed to 1.25 μg/mL of ferutinin and 2 µM of E2 at 2 dpf, which confirmed the result of histology analysis. In addition, RT-qPCR of high concentration of ferutinin and E2 demonstrated that bmp2a/b and esr1 were downregulated and upregulated when the larvae were exposed to 5 μg/mL of ferutinin and 10 µM of E2, respectively.

scholarly journals Auditory Brainstem Responses (ABR) of Rats during Experimentally Induced Tinnitus: Literature Review

2020 ◽  
Vol 10 (12) ◽  
pp. 901
Author(s):  
Ewa Domarecka ◽  
Heidi Olze ◽  
Agnieszka J. Szczepek
Keyword(s):  
Rat Model ◽  
Basic Research ◽  
Auditory Brainstem ◽  
Auditory Brainstem Responses ◽  
Consistent Finding ◽  
Mesh Terms ◽  
Brainstem Response ◽  
Experimental Protocols ◽  
High Level ◽  
Experimentally Induced

Tinnitus is a subjective phantom sound perceived only by the affected person and a symptom of various auditory and non-auditory conditions. The majority of methods used in clinical and basic research for tinnitus diagnosis are subjective. To better understand tinnitus-associated changes in the auditory system, an objective technique measuring auditory sensitivity—the auditory brainstem responses (ABR)—has been suggested. Therefore, the present review aimed to summarize ABR’s features in a rat model during experimentally induced tinnitus. PubMed, Web of Science, Science Direct, and Scopus databanks were searched using Medical Subject Heading (MeSH) terms: auditory brainstem response, tinnitus, rat. The search identified 344 articles, and 36 of them were selected for the full-text analyses. The experimental protocols and results were evaluated, and the gained knowledge was synthesized. A high level of heterogeneity between the studies was found regarding all assessed areas. The most consistent finding of all studies was a reduction in the ABR wave I amplitude following exposure to noise and salicylate. Simultaneously, animals with salicylate-induced but not noise-induced tinnitus had an increased amplitude of wave IV. Furthermore, the present study identified a need to develop a consensus experimental ABR protocol applied in future tinnitus studies using the rat model.

scholarly journals Innate Immune Response to Streptococcus iniae Infection in Zebrafish Larvae

2012 ◽  
Vol 81 (1) ◽  
pp. 110-121 ◽  
Author(s):  
Elizabeth A. Harvie ◽  
Julie M. Green ◽  
Melody N. Neely ◽  
Anna Huttenlocher
Keyword(s):  
Systemic Infection ◽  
Neutrophil Function ◽  
Otic Vesicle ◽  
Streptococcus Iniae ◽  
Wild Type ◽  
Zebrafish Model ◽  
Content Type ◽  
Larval Zebrafish ◽  
Zebrafish Larvae ◽  
Lethal Infection

Streptococcus iniaecauses systemic infection characterized by meningitis and sepsis. Here, we report a larval zebrafish model ofS. iniaeinfection. Injection of wild-typeS. iniaeinto the otic vesicle induced a lethal infection by 24 h postinfection. In contrast, anS. iniaemutant deficient in polysaccharide capsule (cpsAmutant) was not lethal, with greater than 90% survival at 24 h postinfection. Live imaging demonstrated that both neutrophils and macrophages were recruited to localized otic infection with mutant and wild-typeS. iniaeand were able to phagocytose bacteria. Depletion of neutrophils and macrophages impaired host survival following infection with wild-typeS. iniaeand thecpsAmutant, suggesting that leukocytes are critical for host survival in the presence of both the wild-type and mutant bacteria. However, zebrafish larvae with impaired neutrophil function but normal macrophage function had increased susceptibility to wild-type bacteria but not thecpsAmutant. Taking these findings together, we have developed a larval zebrafish model ofS. iniaeinfection and have found that although neutrophils are important for controlling infection with wild-typeS. iniae, neutrophils are not necessary for host defense against thecpsAmutant.

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