Quantitative Evaluation of Porphyromonas gingivalis in Indian Subjects With Chronic Periodontitis by Real-Time Polymerase Chain Reaction

2019 ◽  
Vol 10 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Manohar S. Kugaji ◽  
Uday M. Muddapur ◽  
Kishore G. Bhat ◽  
Vinayak M. Joshi ◽  
Vijay M. Kumbar ◽  
...  
Keyword(s):  
Real Time ◽  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Alveolar Bone ◽  
Microbiological Analysis ◽  
Rt Pcr ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Significant Difference ◽  
Attachment Level

Background and Aims: Porphyromonas gingivalis ( P. gingivalis) is considered as an important pathogen responsible for periodontal disease which is characterized by inflammation of gingiva and destruction of periodontal ligament and alveolar bone leading to loss of tooth. Along with clinical investigations, suitable microbiological analysis needs to be performed which could provide more insight into the disease severity. We aim to quantify P. gingivalis by real-time PCR (RT-PCR) and analyze its association with demographic data including clinical parameters. Materials and Methods: The study consisted of chronic periodontitis patients (CP group) and healthy subjects (H group) with 120 samples in each group. RT-PCR was carried out by the SYBR Green assay to target 16S ribosomal ribonucleic acid species-specific region of P. gingivalis. Standard strain of P. gingivalis ATCC 33277 was used as a control. Results: In the CP group, 79.16% samples were found positive for P. gingivalis, whereas 29.17% samples were positive in the H group. A significant difference was found when the prevalence was compared within males and females ( P < .001 for both). In the older age groups, we found a higher rate of detection of P. gingivalis. As analyzed by Spearman’s correlation test, the number of cells of P. gingivalis was significantly associated with probing depth ( P = .02) and clinical attachment level ( P = .01) in the CP group. The mean cell number of P. gingivalis was found to be increasing with increasing levels of probing depth and clinical attachment level ( P < .001 and P = .01, respectively). Conclusion: The present study reaffirms that the P. gingivalis microbe is significantly associated with the chronic periodontitis and that its level varies with the severity of the disease. Colonization of the bacterium is significantly associated with severe forms of the disease.

scholarly journals Laser Assisted Non-surgical Periodontal Therapy: A Double Blind, Randomized Clinical Trial

2017 ◽  
Vol 11 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Joseph D. Everett ◽  
Jeffrey A. Rossmann ◽  
David G. Kerns ◽  
Ibtisam Al-Hashimi
Keyword(s):  
Laser Therapy ◽  
Chronic Periodontitis ◽  
Test Group ◽  
Control Group ◽  
Scaling And Root Planing ◽  
Clinical Attachment Level ◽  
Laboratory Parameters ◽  
Root Planing ◽  
Significant Difference ◽  
Attachment Level

The objective of this study was to examine potential benefits of using laser therapy for secular decontamination in conjunction with scaling and root planing in the treatment of chronic periodontitis. The study was performed on 173 teeth in 14 patients in a split-mouth design, one side received scaling and root planing followed by laser therapy using a carbon dioxide (CO2) laser with an ablative handpiece (test group); the contralateral side received scaling and root planing without laser (control group). Clinical and laboratory parameters were evaluated prior to treatment and at 3 and 6 months following therapy; clinical measurements were performed by two blinded examiners. The clinical parameters included measurement of gingival recession (REC), bleeding on probing (BOP), clinical attachment level (CAL), pocket depth (PD), furcation involvement (FUR), and tooth mobility (MOB). Laboratory testing to determine the levels of periodontal pathogens was performed using PCR techniques. The results of the study revealed statistically significant differences in clinical and laboratory parameters at 3 and 6 months after therapy for both test and control groups, but no significant difference was observed between the two groups. However, sites receiving laser therapy tended to show a greater decrease in probing depths, gain in clinical attachment level, and reduced bacterial levels. In conclusion, the overall results of the study suggest a potential benefit of using laser therapy in conjunction with scaling and root planing for the treatment of chronic periodontitis.

scholarly journals YES! OXIDATIVE STRESS MAY BE A RISK FACTOR FOR CHRONIC PERIODONTITIS PATIENTS

2020 ◽  
pp. 1-3
Author(s):  
Archith Mannan ◽  
Mathew John ◽  
Bindu Rachel Thomas ◽  
Sabari Chandramohan ◽  
Aswathy S. ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Periodontitis ◽  
Defense Mechanisms ◽  
Alveolar Bone ◽  
Inflammatory Disorder ◽  
Complex Interactions ◽  
Probing Depth ◽  
Whole Saliva ◽  
Potential Damage ◽  
Attachment Level

Oxidative stress is defined as the condition occurring when the physiological balance between oxidants and antioxidants is disrupted in favor of the former with potential damage for the organism. The pathological events leading to the destruction of the periodontium encompasses complex interactions involving an imbalance between oxidant and antioxidant enzymatic and non-enzymatic defense mechanisms. Periodontitis is an oral inflammatory disorder affecting alveolar bone, gingiva, and periodontal ligament initiated by the complex interaction between the presence of microorganisms (periodontopathic bacteria) and the host’s immune response. Whole saliva samples were collected from 25 chronic periodontitis patients. Saliva samples were centrifuged and the supernatant was used to determine the 8-OHdG levels with a competitive ELISA kit. To determine the clinical condition of each subject, the Bleeding on Probing (BOP), Clinical attachment level (CAL), and Pocket probing depth (PPD) were measured. This study shows significant correlation between increased values of oxidative stress biomarker and clinical parameters in chronic periodontitis patients.

scholarly journals Transmission of Aggregatibacter actinomycetemcomitans between Brazilian women with severe chronic periodontitis and their children

2007 ◽  
Vol 18 (3) ◽  
pp. 220-224 ◽  
Author(s):  
Rodrigo Otávio Citó César Rêgo ◽  
Denise Madalena Palomari Spolidorio ◽  
Sérgio Luiz de Souza Salvador ◽  
Joni Augusto Cirelli
Keyword(s):  
Chronic Periodontitis ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Selective Medium ◽  
Child Transmission ◽  
Clinical Attachment Level ◽  
Gram Staining ◽  
Probing Depth ◽  
Polymerase Chain ◽  
Attachment Level ◽  
Transport Fluid

This study evaluated the transmission of Aggregatibacter actinomycetemcomitans (Aa) in women with severe chronic periodontitis and their children. Thirty women (mean age = 36.1±6.0 years) who were mothers of at least one child aged 7 to 16 years were enrolled. In order to investigate mother-child transmission of Aa, the children were also evaluated when their mothers were colonized by the bacterium. Subgingival plaque samples of each woman were collected from 3 sites (mean probing depth of 7.3±1.2 mm and mean clinical attachment level of 7.9±1.5 mm) and pooled in reduced transport fluid (RTF). These samples were processed, inoculated onto TSBV-agar selective medium and incubated at 37°C in microaerophilic atmosphere for 5 days. Aa was identified on the basis of colony morphology, Gram staining, catalase and oxidase reactions. Aa was found in 8 out of 30 women. Therefore, 8 children from these women (mean age= 12 ± 3.7 years) were evaluated, but Aa was found only in 2 of them. Aa strains of the two mother-child pairs were evaluated by arbitrarily-primed polymerase chain reaction (AP-PCR), although it was not found similarity between the amplitypes of each pair. No Aa transmission was found between Brazilian women with severe chronic periodontitis and their children.

Are Teeth Close to the Cleft More Susceptible to Periodontal Disease?

2009 ◽  
Vol 46 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Ana Lúcia Pompéia Fraga de Almeida ◽  
Marly Kimie Sonohara Gonzalez ◽  
Sebastião Luiz Aguiar Greghi ◽  
Paulo César Rodrigues Conti ◽  
Luiz Fernando Pegoraro
Keyword(s):  
Periodontal Disease ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Craniofacial Anomalies ◽  
Arithmetic Means ◽  
Cross Sectional ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Significant Difference ◽  
Attachment Level

Objective: To evaluate whether teeth close to the cleft area present higher prevalence and severity of periodontal disease than teeth in other regions. Design: Cross-sectional. Setting: Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo. Patients: There were 400 individuals with complete unilateral or bilateral cleft lip and palate, aged 15 to 49 years, without any previous periodontal treatment. Main Outcome Measures: All clinical parameters were evaluated in six sites for each tooth. The arithmetic means were calculated for each sextant. Results: Of the sextants, 86.75% presented means of probing depth smaller than or equal to 3 mm. No sextant exhibited means of probing depth greater than or equal to 6 mm. There was a statistically significant difference (p < .001) in probing depth according to age, types of cleft, and sextant; 95.87% of sextants presented mean attachment levels smaller than or equal to 3 mm. The sextant with cleft did not present higher means of probing depth, clinical attachment level, plaque index, and gingival index. There was gingival bleeding in 99.08% of the sample and plaque in 97.40%. The type of cleft was not an important factor influencing the prevalence of periodontal disease. Age seems to be an important factor influencing the prevalence and severity of periodontal disease for all aspects investigated. Conclusions: Periodontal disease in individuals with clefts occurred in a similar manner as observed in other populations. The presence of the cleft does not seem to increase the prevalence of the disease.

scholarly journals Histopathological Features of Dental Pulp in Teeth with Different Levels of Chronic Periodontitis Severity

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Elizangela Partata Zuza ◽  
Ana Luiza Vanzato Carrareto ◽  
Raphael Carlos Comelli Lia ◽  
Juliana Rico Pires ◽  
Benedicto Egbert Corrêa de Toledo
Keyword(s):  
Chronic Periodontitis ◽  
Alveolar Bone ◽  
Clinical Attachment Level ◽  
Single Root ◽  
Tooth Apex ◽  
Group 3 ◽  
Group 2 ◽  
Attachment Level ◽  
Different Levels ◽  
Group 1

Purpose. To evaluate the histopathological condition of the pulp in teeth with different levels of chronic periodontitis in humans. Methods. Twenty-five single-root nondecayed teeth were divided into three groups as follows: group 1, clinical attachment level (CAL) 3 to 4 mm and alveolar bone loss (BL) from 4 to 6 mm without reaching the tooth apex; group 2, CAL≥5 mm and BL>6 mm without reaching the tooth apex; group 3, CAL≥5 mm and BL>6 mm up to the tooth apex. Histological analyses were accomplished after laboratorial processing. Results. The mean of CAL was 3.2±0.7 mm in group 1, 7.6±2.0 mm in group 2, and 12.1±2.8 mm in group 3, while for BL it was 4.8±0.9 mm, 7.6±2.2 mm, and 11.9±2.1 mm, respectively. Histopathological data in the pulpal chambers were similar among the three groups showing normal aspects, and, the radicular pulps showed variable levels of reactive dentin, fibrosis, dystrophic mineralizations, atrophy, and mononuclear inflammatory infiltrate. Conclusions. Gradual progression of the chronic periodontitis led to changes in the histopathological aspects of the radicular pulp with progressive involvement.

scholarly journals Periodontal Outcomes in Liver Transplantation Individuals: A Systematic Review and Meta-analysis

2021 ◽  
Vol 30 (1) ◽  
pp. 122-131
Author(s):  
Tuélita Marques Galdino ◽  
Carla Souza Oliveira ◽  
Allyson Nogueira Moreira ◽  
Francisco Ivison Rodrigues Limeira ◽  
Cláudia Silami Magalhães ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Meta Analysis ◽  
Grey Literature ◽  
Mean Difference ◽  
Control Group ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Meta Analyses ◽  
And Control ◽  
Attachment Level

Aim: To systematically review the literature for observational studies evaluating periodontal outcomes in pre- and post-liver transplant (LT) individuals compared to a control group. Methods: Specific strategies were designed to search five databases and the grey literature. Hand-searches were also performed. The following periodontal outcomes were analyzed in pre-LT, post-LT and control groups: plaque index (PI), gingival index (GI), gingival growth (GO), alveolar bone loss (ABL) probing depth (PD) and clinical attachment level (CAL). Meta-analyses and subgroup analyses were conducted. Results: Among the 257 studies retrieved, 11 studies were included. Four studies were incorporated into the meta-analysis and showed that PI (mean difference=0.15, 95%CI: 0.12-0.18) and GI (mean difference=0.31, 95%CI: 0.06-0.56) were significantly higher in post-LT individuals compared to the control group. There was no significant mean difference of PD (mean difference=0.49, 95%CI: -0.26-1.25) but CAL (mean differ- ence=1.47, 95%CI: 0.19-2.75) were significantly higher in pre-LT individuals compared to the control group. Conclusion: The higher means of PI and GI in post-LT and CAL in pre-LT compared to healthy individuals suggest a relationship between the periodontal condition and the transplantation status.

scholarly journals Clinical Assessment of Drug Adjunctive Therapy Effects in Association with Chronic Generalized Periodontitis and Osteoporotic Disease

2015 ◽  
Vol 19 (3) ◽  
pp. 158-162
Author(s):  
Irina-Georgeta Ursarescu ◽  
Liliana Pasarin ◽  
Sorina Solomon ◽  
Alexandra Martu ◽  
Silvia Martu
Keyword(s):  
Chronic Periodontitis ◽  
Adjunctive Therapy ◽  
Control Group ◽  
Study Group ◽  
Clinical Effects ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Periodontal Debridement ◽  
Significant Clinical Improvement ◽  
Attachment Level

SummaryAim: The present study proposes an assessment of the clinical effects on periodontal level generated by the adjunctive periodontal therapy with sub-antimicrobial doses of doxycycline in patients with chronic periodontitis and osteoporosis.Materials and Methods: The study group comprised 26 patients with chronic periodontitis and osteoporosis, divided in the study group (classical periodontal debridement and sub-antimicrobial doses of doxycycline for 3 months) and the control group (classical debridement only). We analyzed the periodontal parameters (probing depth, clinical attachment level, PBI and PI indices) at baseline, in the last day of medication and at 3 months after the drug therapy.Results and Discussion: The reduction of the moderate and profound pockets was higher for the group with drug adjunctive therapy. The sites with an initial depth of 0-3mm in the study group presented also a slight attachment gain.Conclusions: The therapy with sub-antimicrobial doses of doxycycline generated significant clinical improvement in patients with chronic periodontitis and osteoporosis, an improvement which can reduce the necessity of surgical procedures.

scholarly journals Evaluation the prevalence of moderate chronic periodontitis among smokers and non- smokers in Aljouf province , Kingdom Of Saudi Arabia

2018 ◽  
Vol 2 (3) ◽  
pp. 145 ◽  
Author(s):  
Ahmed Shawkat Hashem ◽  
Hesham Shokry Ismail ◽  
Santosh R Patil ◽  
Mohammad Khursheed Alam
Keyword(s):  
Chronic Periodontitis ◽  
Limited Data ◽  
Significant Relation ◽  
Kingdom Of Saudi Arabia ◽  
Chi Square ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Chi Square Test ◽  
Special Examination ◽  
Attachment Level

Background: Due to limited data about the prevalence of the moderate chronic periodontitis among smokers and non- smokers in Saudi population this study was conducted.Methods: For each participant, full mouth periodontal examination was performed and recorded on a special examination form by one of the 3 examiners. These data included probing depth (PD), clinical attachment level (CAL). The Data was collected and entered into the personal computer. Statistical analysis was done using Statistical Package for Social Sciences (SPSS/version 21) software. Number and percent of each category were calculated, for categorized parameters, chi square test was used. The level of significant was 0.05.Results: On comparison the prevalence of moderate chronic periodontitis among the smoker and non- smoker groups, chi square test was 9.114, with ( p< 0.05) which is statistically significant, while on comparison the age between the two groups, it was found that there was no significant relation between the age and the different grade of chronic periodontitis.Conclusion: Moderate Chronic Periodontitis is more prevalent among smokers in Aljouf province, Kingdom of Saudi Arabia.International Journal of Human and Health Sciences Vol. 02 No. 03 July’18. Page : 145-148

scholarly journals Increased Levels of 8-Hydroxydeoxyguanosine and Malondialdehyde and its Relationship with Antioxidant Enzymes in Saliva of Periodontitis Patients

2009 ◽  
Vol 03 (02) ◽  
pp. 100-106 ◽  
Author(s):  
Cenk Fatih Canakci ◽  
Yasin Cicek ◽  
Abdulkadir Yildirim ◽  
Ufuk Sezer ◽  
Varol Canakci
Keyword(s):  
Antioxidant Enzymes ◽  
Chronic Periodontitis ◽  
Antioxidant Activities ◽  
Clinical Attachment Level ◽  
Periodontal Inflammation ◽  
Probing Depth ◽  
Whole Saliva ◽  
Healthy Control ◽  
The Relationship ◽  
Attachment Level

ABSTRACTObjectives: The aim of this study was to evaluate 8-hydroxydeoxyguanosine (8-OHdG) and Malondialdehyde (MDA) levels, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in whole saliva of patients with chronic periodontitis. Moreover, the relationship among the oxidative damage biomarkers, antioxidant enzymes activities and clinical periodontal status were investigated.Methods: Whole saliva samples were collected from 30 patients with chronic periodontitis and 30 periodontally healthy control. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level, and probing depth were measured. The salivary 8-OHdG level was measured using the ELISA method. SOD and GPx activities and MDA levels were determined spectrophotometrically.Results: Higher salivary 8-OHdG and MDA levels (P<.001), and lower salivary SOD and GPx activities (P<.05) were detected in periodontitis patients compared to the healthy controls. Additionally, there were significantnegative correlations between salivary levels of 8-OHdG and both salivary SOD and GPx activities as well as between salivary levels of MDA and both salivary SOD and GPx activities (P<.001).Conclusions: Higher salivary 8-OHdG and MDA levels and lower salivary antioxidant activities seem to reflect increased oxygen radical activity during periodontal inflammation. (Eur J Dent 2009;3:100- 106)

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