scholarly journals Peculiarities of cytochemical properties of cancer cells as revealed by study of deoxyribonucleoprotein susceptibility to Feulgen hydrolysis.

1977 ◽  
Vol 25 (7) ◽  
pp. 580-584 ◽  
Author(s):  
A V Zelenin ◽  
A A Kushch ◽  
T A Chebanu
Keyword(s):  
Acid Hydrolysis ◽  
Cancer Cells ◽  
Deoxyribonucleic Acid ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Feulgen Reaction ◽  
Squamous Carcinoma Cells ◽  
Schiff Reagent ◽  
Increased Sensitivity ◽  
Absorption Technique

Chromatin of human squamous carcinoma cells reacts more intensively to short (1-2 min) acid hydrolysis in the Feulgen reaction and is, after such treatment, more intensively stained by Schiff reagent than chromatin of normal cells of the same origin. To reveal this difference in chromatin properties the use of a fluorescence variant of the Feulgen reaction is necessary because nuclei-binding of Schiff reagent after such short hydrolysis is so weak that the amount of the stain bound by means of absorption technique is hardly possible. The use of increased sensitivity of cancer cells chromatin to acid hydrolysis for cancer cytodiagnosis is suggested, especially for the diagnosis of so called diploid cancers for which detection on the basis of deoxyribonucleic acid content determination is impossible.

scholarly journals The Migration and Invasion of Oral Squamous Carcinoma Cells: Matrix, Growth Factor and Signalling Involvement

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2633
Author(s):  
Ian R. Ellis
Keyword(s):  
Growth Factor ◽  
Cancer Cells ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Squamous Carcinoma Cells ◽  
Oral Squamous Carcinoma

The link between the migration of cancer cells and the spread of cancers has been established for many years [...]

scholarly journals Capsaicin Potentiates Anticancer Drug Efficacy Through Autophagy-Mediated Ribophorin II Downregulation and Necroptosis in Oral Squamous Cell Carcinoma Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Yi-Ching Huang ◽  
Tien-Ming Yuan ◽  
Bang-Hung Liu ◽  
Kai-Li Liu ◽  
Chiung-Hua Wung ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Anticancer Drugs ◽  
Apoptotic Cell ◽  
Squamous Carcinoma ◽  
Chemotherapeutic Agents ◽  
Carcinoma Cells ◽  
Down Regulation ◽  
Squamous Carcinoma Cells ◽  
Oral Squamous Carcinoma

The ability of capsaicin co-treatment to sensitize cancer cells to anticancer drugs has been widely documented, but the detailed underlying mechanisms remain unknown. In addition, the role of ribophorin II turnover on chemosensitization is still uncertain. Here, we investigated capsaicin-induced sensitization to chemotherapeutic agents in the human oral squamous carcinoma cell lines, HSC-3 and SAS. We found that capsaicin (200 μM) did not induce remarkable apoptotic cell death in these cell lines; instead, it significantly enhanced autophagy with a concomitant decrease of ribophorin II protein. This capsaicin-induced decrease in ribophorin II was intensified by the autophagy inducer, rapamycin, but attenuated by the autophagy inhibitors, ULK1 inhibitor and chloroquine, indicating that the autophagic process was responsible for the capsaicin-induced down-regulation of ribophorin II. Co-administration of capsaicin with conventional anticancer agents did, indeed, sensitize the cancer cells to these agents. In co-treated cells, the induction of apoptosis was significantly reduced and the levels of the necroptosis markers, phospho-MLKL and phospho-RIP3, were increased relative to the levels seen in capsaicin treatment alone. The levels of DNA damage response markers were also diminished by co-treatment. Collectively, our results reveal a novel mechanism by which capsaicin sensitizes oral cancer cells to anticancer drugs through the up-regulation of autophagy and down-regulation of ribophorin II, and further indicate that the induction of necroptosis is a critical factor in the capsaicin-mediated chemosensitization of oral squamous carcinoma cells to conventional anticancer drugs.

Inhibition of SOX15 Sensitizes Esophageal Squamous Carcinoma Cells to Paclitaxel

2019 ◽  
Vol 19 (5) ◽  
pp. 349-356 ◽  
Author(s):  
Ming Zhang ◽  
Jianying Wang ◽  
Tianwei Gao ◽  
Xin Chen ◽  
Yan Xu ◽  
...  
Keyword(s):  
Cell Fate ◽  
Expression Patterns ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Squamous Carcinoma Cells ◽  
Expression Of Genes ◽  
Therapeutic Outcomes ◽  
Esophageal Squamous Cell Carcinomas ◽  
Increased Sensitivity ◽  
Esophageal Squamous Carcinoma

Background: SOX15 is a crucial transcription factor involved in the regulation of embryonic development and in the cell fate determination. It is also an important mediator of tumorigenesis in cancer. Methods: Here, we sought to explore the expression patterns and biological functions of SOX15 in esophageal squamous cell carcinomas (ESCC). SOX15 was found aberrantly overexpressed in ESCC tumors. Results: Experimentally, inhibition of SOX15 through RNAi suppressed cell proliferation in ESCC cells and sensitized cancer cells to paclitaxel, but not to Cisplatin. Moreover, inhibition of SOX15 significantly repressed the expression of genes associated with WNT and NOTCH signaling pathways, which may contribute to the increased sensitivity to paclitaxel. Conclusion: In conclusion, the current study revealed that inhibition of SOX15 in ESCC cells sensitizes the ESCC cells to paclitaxel, suggesting that the SOX15 expression level may predict the therapeutic outcomes for paclitaxel treatment for ESCC.

scholarly journals Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

2021 ◽  
Vol 11 (8) ◽  
pp. 3524
Author(s):  
Azeem Ul Yaqin Syed ◽  
Muhammad A. Ahmed ◽  
Eman I. AlSagob ◽  
Mansour Al-Askar ◽  
Abdulrahman M. AlMubarak ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Control Cell ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Muscle Actin ◽  
Catha Edulis ◽  
Squamous Carcinoma Cells ◽  
Dose Dependent

The aim was to determine the cytotoxicity of Khat (Catha edulis (Vahl) Forssk. ex Endl) on normal oral fibroblasts (NOFs) and SCC4 (squamous carcinoma cells) along with expression of α-smooth muscle actin (α-SMA) in fibroblasts. Khat filtrate was prepared to obtain a concentrated viscous solution. NOFs and SCC4 cells were cultured in biological cabinets and were grown in Dulbeccos’ modified Eagles medium. Frozen cells were thawed at 37 °C and cell seeding was performed. NOFs and SCC4 cells were seeded on 96 well plates and allowed to attach. The medium was removed and a fresh medium containing different concentrations of Khat was added. The group without Khat served as a negative control and 4% paraformaldehyde as the positive control. Cell viability was assessed using the MTT assay and effect of Khat on fibroblast and SCC4 phenotypes was evaluated by immunostaining. Analysis of variance was used to assess data (p < 0.05). NOF 316 showed cell death in response to 4% paraformaldehyde, 12.5, 6.25, and 3.12 mg/mL of Khat. The highest concentration of Khat (25 mg/mL) failed to cause cytotoxicity of NOF 316. NOF 319 and NOF 26 displayed cell death at all concentrations of Khat, however, cytotoxicity was not dose dependent. NOF 18 and SCC4 cells showed dose-dependent cell death. NOF 316 showed α-SMA expression after 1 mg/mL of Khat exposure. Not all fibroblasts were α-SMA-positive, suggesting specific activation of a subset of fibroblasts. Khat is cytotoxic to NOF and SCC4 cells. Furthermore, it can also cause activation and phenotypic changes in oral fibroblasts, indicating a potential role in progression of oral squamous cell carcinoma.

Upregulation of HO-1 is accompanied by activation of p38MAPK and mTOR in human oesophageal squamous carcinoma cells

2013 ◽  
Vol 37 (6) ◽  
pp. 584-592 ◽  
Author(s):  
Jian-Li Hu ◽  
Lan Xiao ◽  
Zhen-Yun Li ◽  
Qiong Wang ◽  
Yu Chang ◽  
...  
Keyword(s):  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Squamous Carcinoma Cells

Improved gene transfer to esophageal adenocarcinoma and squamous carcinoma cells using targeted adenovirus vectors

2001 ◽  
Vol 120 (5) ◽  
pp. A11
Author(s):  
Willem A. Marsman ◽  
Christianne J. Buskens ◽  
John G. Wesseling ◽  
Hidde J. Haisma ◽  
David T. Curiel ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Esophageal Adenocarcinoma ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Squamous Carcinoma Cells ◽  
Adenovirus Vectors
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