Lack of Effect of Frost-Free Refrigeration on the Volume of Antibiotic Liquids

1996 ◽  
Vol 12 (1) ◽  
pp. 21-23
Author(s):  
John Erramouspe
Keyword(s):  
Storage Conditions ◽  
The Other ◽  
Expected Number ◽  
Volume Depletion ◽  
Plastic Bags ◽  
Plastic Bag ◽  
The Mean ◽  
Clinically Significant

Objective: To determine whether sealing liquid penicillin bottles in resealable plastic bags during storage in a household frost-free refrigerator prevents clinically significant evaporative losses. Design: Sixty bottles of penicillin VK 250 mg/5 mL, 100 mL (10 bottles from each of 6 different manufacturers: Apothecon, Biocraft, Lederle, SmithKline Beecham, Warner-Chilcott, and Wyeth-Ayerst) were mixed according to the manufacturers' recommendations. Five bottles from each manufacturer were stored inside a resealable plastic bag and the other 5 bottles were stored outside a bag (both at 2–8 °C in a frost-free refrigerator). Twice daily, 5-mL doses were removed from the bottles. The mean total volume removed from bottles stored inside resealable plastic bags was compared with that from bottles stored outside bags. Results: There was no difference in the average total amount removed from bottles between the two storage conditions. Conclusions: Sealing bottles containing liquid penicillin in plastic bags during storage in a household frost-free refrigerator seems to offer no advantage in protection from volume depletion via evaporation over 10 days. Factors other than evaporation are probably responsible when antibiotics that require refrigeration have insufficient volume to provide the expected number of doses.

scholarly journals Eggs viability of Aedes aegypti Linnaeus (Diptera, Culicidae) under different environmental and storage conditions in Manaus, Amazonas, Brazil

2016 ◽  
Vol 77 (2) ◽  
pp. 396-401 ◽  
Author(s):  
V. C. Soares-Pinheiro ◽  
W. Dasso-Pinheiro ◽  
J. M. Trindade-Bezerra ◽  
W. P. Tadei
Keyword(s):  
Aedes Aegypti ◽  
Storage Conditions ◽  
Amazon Region ◽  
Storage Site ◽  
Egg Hatching ◽  
Plastic Bag ◽  
The Mean ◽  
And Storage ◽  
Storage Type ◽  
Hatching Rates

Abstract The viability of Aedes aegypti eggs was assessed in the Amazon region. The eggs were maintained under different conditions: indoors (insectarium) and outdoors (natural environment), as well as in different storage types (plastic cup, paper envelope, plastic bag) for different days. Egg viability was measured as the mean of hatchings observed from egg-bearing sheets of filter paper immersed in water, using three sheets randomly selected from each storage type and at both sites. There were significant differences in the viability of Ae. aegypti eggs with respect to the location (F=30.40; DF=1; P<0.0001), storage type (F=17.66; DF=2; P<0.0001), and time of storage (F=49.56; DF=9; P<0.0001). The interaction between storage site versus storage type was also significant (F=15.96; DF=2; P<0.0001). A higher hatching mean was observed for the eggs kept in the insectarium than for those outdoors (32.38 versus 7.46). Hatching rates of egg batches stored for 12 to 61 days ranged between 84 and 90%. A reduction was observed between 89 and 118 days, with values of 63 and 48%, respectively. With respect to type of storage, mean egg hatching was higher for the eggs in plastic cups (44.46). It was concluded that the viability of the eggs of Ae. aegypti in the Amazon region remains high up to 4 months, after which it declines drastically, although in this study hatching occurred for up to 8 months in very low percentages.

Platelet Gel for the Surgical Treatment of Onychocryptosis

2008 ◽  
Vol 98 (4) ◽  
pp. 296-301 ◽  
Author(s):  
Antonio Córdoba-Fernandez ◽  
Rafael Rayo-Rosado ◽  
José María Juarez-Jiménez
Keyword(s):  
Clinical Trial ◽  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Positive Control ◽  
The Other ◽  
Platelet Gel ◽  
Experimental Conditions ◽  
The Mean ◽  
Clinically Significant ◽  
Mean Time

Background: The growth factors derived from platelets contained in platelet-rich plasma comprise a series of molecules that favor the production of collagen with the proliferation of fibroblasts and new blood vessels. These substances exert their effect on the cells, acting in all the stages of cicatrization, especially in hemostasis and early fibroplasia. Methods: Thirty-five patients (70 feet) were selected, operated on both sides for ingrown hallux nails, and subjected to two different experimental conditions in a crossover clinical trial with positive control of treatment. Two main variables were analyzed: on one side, the bleeding, according to three preestablished categories, and on the other, the mean time of cicatrization in days. Results: Significant differences (P &lt; .001) were found between the two groups for bleeding. We observed that cicatrization time did not differ significantly between the two treatments. Conclusion: The use of platelet gel for the treatment of onychocryptosis by single nonincisional matricectomy can guarantee good hemostasis, with a significant reduction in bleeding, but does not produce a clinically significant reduction in cicatrization time. (J Am Podiatr Med Assoc 98(4): 296–301, 2008)

scholarly journals The Mean Value Theorem for Integrals Method for Estimating Two-Dimensional Renewal Functions

2020 ◽  
Vol 4 (1) ◽  
pp. 49
Author(s):  
Leopoldus Ricky Sasongko ◽  
Bambang Susanto
Keyword(s):  
Weibull Model ◽  
Mean Value ◽  
The Other ◽  
Mean Value Theorem ◽  
Two Dimensional ◽  
Failure Model ◽  
Expected Number ◽  
Warranty Period ◽  
Bivariate Data ◽  
The Mean

An important aspect in the provision of a two-dimensional warranty is the expected number of failures of a component during the two-dimensional warranty period. The purpose of this paper is to present a new method to obtain the expected number of failures of a nonrepairable compo­nent from the two-dimensional renewal functions as the so­lution of two-dimensional renewal integral equations through the Mean Value Theorem for Integrals (MeVTI) method. The two-dimensional renewal integral equation involves Lu-Bhattacharyya’s bivariate Weibull model as a two-dimensional failure model. It turns out that the estimation of the expected number of failures using the MeVTI method is close to that of the other method, Riemann-Stieljies method. The bivariate data behaviour of the failures of an automobile component is also studied in this paper.

scholarly journals Qualidade pós-colheita de tubérculos de batata ‘Ágata’ embalados armazenados em diferentes condições

2019 ◽  
Author(s):  
Poliana Prates de Souza Soares ◽  
Rosane Mendonça do Nascimento ◽  
Paula Acácia Silva Ramos ◽  
Ranyelly Leão Coutrim ◽  
Tâmara Moreira Silva ◽  
...  
Keyword(s):  
Vitamin C ◽  
Raw Materials ◽  
Titratable Acidity ◽  
Storage Conditions ◽  
Soluble Solids ◽  
Potato Tubers ◽  
Ambient Conditions ◽  
Plastic Bags ◽  
Randomized Design ◽  
Plastic Bag

The adequate storage of raw materials is fundamental to obtain a quality final product. The objective of this study was to evaluate the effect of different plastic packages on the maintenance of post-harvest quality of ‘Agata’ potato tubers stored under refrigeration and room temperature. Two experiments were conducted, both with a completely randomized design, arranged in a 3 x 4 factorial scheme (three types of packages – unpacked, with polyvinyl chloride film – PVC (21 cm x 40 cm and 12 μm thick) and with plastic bag (30 cm x 40 cm and 30 μm thick) and four evaluation times), with three replicates of one tuber each. In one experiment the samples were maintained under ambient conditions (25 ± 2°C and 60% ± 5% RH), with evaluations performed at 0, 7, 14 and 21 days, and another under refrigeration (8 ± 2ºC 75% ± 2% RH) and 0, 25, 50 and 75 days. The evaluated characteristics were: firmness, pH, soluble solids, total titratable acidity, vitamin C and the appearance of shoots. Tubers can only be stored under ambient conditions for seven days, and during this period the PVC packaging is the most suitable for storage. Tuber firmness was reduced and the vitamin C content of the last evaluation was higher than the initial content in both storage conditions. The refrigerated storage extended the shelf life of tubers for up to 50 days, period during which the PVC and plastic bags did not influence the conservation of the ‘Agata’ potato tubers.

Immunologic Assessment of Patients Treated with Bovine Fibrin as a Hemostatic Agent

1996 ◽  
Vol 76 (06) ◽  
pp. 0925-0931 ◽  
Author(s):  
John F Carroll ◽  
Keith A Moskowitz ◽  
Niloo M Edwards ◽  
Thomas J Hickey ◽  
Eric A Rose ◽  
...  
Keyword(s):  
Coagulation Factors ◽  
Allergic Reactions ◽  
Factor V ◽  
Normal Range ◽  
Serum Samples ◽  
Human Fibrinogen ◽  
Bovine Thrombin ◽  
Thrombotic Complications ◽  
The Mean ◽  
Clinically Significant

SummaryTwenty-one cardiothoracic surgical patients have been treated with fibrin as a topical hemostatic/sealing agent, prepared from bovine fibrinogen clotted with bovine thrombin. Serum samples have been collected before treatment with fibrin and postoperatively between 1 and 9 days, 3 and 12 weeks, and 6 and 8 months. The titers of anti-bovine fibrinogen antibodies, measured by ELISA specific for immunoglobulins IgG or IgM, increased to maximal values after about 8 or 6 weeks, respectively. After 8 months, IgG titers were on average 20-fold lower than the mean maximal value, while IgM titers returned to the normal range. IgG was the predominant anti-bovine fibrinogen immunoglobulin as documented by ELISA, affinity chromatography and electrophoresis. Anti-bovine fibrinogen antibodies present in patients reacted readily with bovine fibrinogen, but did not cross-react with human fibrinogen as measured by ELISA or by immunoelectrophoresis. A significant amount of antibodies against bovine thrombin and factor V has been found, many cross-reacting with the human counterparts. No hemorrhagic or thrombotic complications, or clinically significant allergic reactions, occurred in any patient, in spite of antibody presence against some bovine and human coagulation factors. The treatment of patients with bovine fibrin, without induction of immunologic response against human fibrinogen, appeared to be an effective topical hemostatic/sealing measure.

Multi-Center Study of Thromboplastin Calibration Precision – Influence of Reagent Species, Composition, and International Sensitivity Index (ISI)

1993 ◽  
Vol 69 (01) ◽  
pp. 035-040 ◽  
Author(s):  
A M H P van den Besselaar ◽  
R M Bertina
Keyword(s):  
Oral Anticoagulants ◽  
International Normalized Ratio ◽  
The Other ◽  
World Health ◽  
Rabbit Brain ◽  
Sensitivity Index ◽  
Bovine Plasma ◽  
Significant Difference ◽  
The Mean ◽  
International Sensitivity Index

SummaryFour thromboplastin reagents were tested by 18 laboratories in Europe, North-America, and Australasia, according to a detailed protocol. One thromboplastin was the International Reference Preparation for ox brain thromboplastin combined with adsorbed bovine plasma (coded OBT/79), and the second was a certified reference material for rabbit brain thromboplastin, plain (coded CRM 149R). The other two thromboplastin reagents were another rabbit plain brain thromboplastin (RP) with a lower ISI than CRM 149R and a rabbit brain thromboplastin combined with adsorbed bovine plasma (RC). Calibration of the latter two reagents was performed according to methods recommended by the World Health Organization (W. H. O.).The purpose of this study was to answer the following questions: 1) Is the calibration of the RC reagent more precise against the bovine/combined (OBT/79) than against the rabbit/plain reagent (CRM 149R)? 2) Is the precision of calibration influenced by the magnitude of the International Sensitivity Index (ISI)?The lowest inter-laboratory variation of ISI was observed in the calibration of the rabbit/plain reagent (RP) against the other rabbit/plain reagent (CRM 149R) (CV 1.6%). The highest interlaboratory variation was obtained in the calibration of rabbit/plain (RP) against bovine/combined (OBT/79) (CV 5.1%). In the calibration of the rabbit/combined (RC) reagent, there was no difference in precision between OBT/79 (CV 4.3%) and CRM 149R (CV 4.2%). Furthermore, there was no significant difference in the precision of the ISI of RC obtained with CRM 149R (ISI = 1.343) and the rabbit/plain (RP) reagent with ISI = 1.14. In conclusion, the calibration of RC could be performed with similar precision with either OBT/79 or CRM 149R, or RP.The mean ISI values calculated with OBT/79 and CRM 149R were practically identical, indicating that there is no bias in the ISI of these reference preparations and that these reference preparations have been stable since their original calibration studies in 1979 and 1987, respectively.International Normalized Ratio (INR) equivalents were calculated for a lyophilized control plasma derived from patients treated with oral anticoagulants. There were small but significant differences in the mean INR equivalents between the bovine and rabbit thromboplastins. There were no differences in the interlaboratory variation of the INR equivalents, when the four thromboplastins were compared.

Prothrombin Time Standardization: Report of the Expert Panel on Oral Anticoagulant Control

1979 ◽  
Vol 42 (04) ◽  
pp. 1073-1114 ◽  
Keyword(s):  
Prothrombin Time ◽  
Expert Panel ◽  
The Other ◽  
Rabbit Brain ◽  
Calibration Constant ◽  
Freeze Dried ◽  
The Mean ◽  
Point Of Intersection ◽  
And Control ◽  
Standardization Report

SummaryIn collaborative experiments in 199 laboratories, nine commercial thromboplastins, four thromboplastins held by the National Institute for Biological Standards and Control (NIBS & C), London and the British Comparative Thromboplastin were tested on fresh normal and coumarin plasmas, and on three series of freeze-dried plasmas. One of these was made from coumarin plasmas and the other two were prepared from normal plasmas; in each series, one plasma was normal and the other two represented different degrees of coumarin defect.Each thromboplastin was calibrated against NIBS&C rabbit brain 70/178, from the slope of the line joining the origin to the point of intersection of the mean ratios of coumarin/normal prothrombin times when the ratios obtained with the two thromboplastins on the same fresh plasmas were plotted against each other. From previous evidence, the slopes were calculated which would have been obtained against the NIBS&C “research standard” thromboplastin 67/40, and termed the “calibration constant” of each thromboplastin. Values obtained from the freeze-dried coumarin plasmas gave generally similar results to those from fresh plasmas for all thromboplastins, whereas values from the artificial plasmas agreed with those from fresh plasmas only when similar thromboplastins were being compared.Taking into account the slopes of the calibration lines and the variation between laboratories, precision in obtaining a patient’s prothrombin time was similar for all thromboplastins.

Platelet Suruival and Function in Rats with Enhanced Thrombotic Tendency

1985 ◽  
Vol 54 (04) ◽  
pp. 739-743 ◽  
Author(s):  
Federica Delaini ◽  
Elisabetta Dejana ◽  
Ine Reyers ◽  
Elisa Vicenzi ◽  
Germana De Bellis Vitti ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Nephrotic Syndrome ◽  
Platelet Function ◽  
Laboratory Tests ◽  
Thrombus Formation ◽  
The Other ◽  
Mean Survival Time ◽  
The Mean ◽  
And Function ◽  
Thrombotic Tendency

SummaryWe have investigated the relevance of some laboratory tests of platelet function in predicting conditions of thrombotic tendency. For this purpose, we studied platelet survival, platelet aggregation in response to different stimuli, TxB2 and 6-keto-PGFlα production in serum of rats bearing a nephrotic syndrome induced by adriamycin. These animals show a heavy predisposition to the development of both arterial and venous thrombosis. The mean survival time was normal in nephrotic rats in comparison to controls. As to aggregation tests, a lower aggregating response was found in ADR-treated rats using ADP or collagen as stimulating agents. With arachidonic acid (AA) we observed similar aggregating responses at lower A A concentrations, whereas at higher AA concentrations a significantly lower response was found in nephrotic rats, despite their higher TxB2 production. Also TxB2 and 6-keto-PGFlα levels in serum of nephrotic rats were significantly higher than in controls. No consistent differences were found in PGI2-activity generated by vessels of control or nephrotic rats.These data show that platelet function may appear normal or even impaired in rats with a markedly increased thrombotic tendency. On the other hand, the significance of high TxB2 levels in connection with mechanisms leading to thrombus formation remains a controversial issue.

METHODOLOGICAL STUDY OF THE RADIOIMMUNOASSAY OF HUMAN THYROTROPHIN

1972 ◽  
Vol 71 (1) ◽  
pp. 24-36 ◽  
Author(s):  
Ariel Gordin ◽  
Pirkko Saarinen
Keyword(s):  
Healthy Subjects ◽  
Binding Capacity ◽  
Paper Electrophoresis ◽  
Storage Conditions ◽  
Methodological Study ◽  
Double Antibody ◽  
The Mean ◽  
Specific Activities ◽  
Initial Binding ◽  
In Pregnancy

ABSTRACT An account is given of a methodological study of the double-antibody radioimmunoassay of human TSH, using highly purified labelled human TSH as tracer. It was shown that conventional paper electrophoresis was not adequate for studying the purity of labelled human TSH. When polyvinylchloride (Pevikon®) electrophoresis was used, four subfractions could still be separated, even though, on paper electrophoresis, the material seemed to be homogeneous. Only two of the four Pevikon fractions were immunoreactive. Purification of labelled human TSH by Pevikon electrophoresis also improved the sensitivity of the assay. Specific activities of about 100 mCi/mg gave the highest initial binding capacity, produced least damage to the labelled hormone and showed the best stability of the tracer without influencing the sensitivity of the method. In different storage conditions, labelled human TSH was found to be most stable at −20°C and diluted 1/100. Only in pregnancy did the addition of HCG seem necessary. The mean TSH value in healthy subjects was 3.6 ± 1.4 μU/ml (mean±sd) with a range from 1.6 μU/ml to 8.8 μU/ml.

Voronoi Analysis of a Soccer Game

2004 ◽  
Vol 9 (3) ◽  
pp. 233-240 ◽  
Author(s):  
S. Kim
Keyword(s):  
Collective Behavior ◽  
Quantitative Assessment ◽  
The Other ◽  
Analysis Method ◽  
Random System ◽  
Soccer Game ◽  
Voronoi Polygons ◽  
The Mean

This paper describes a Voronoi analysis method to analyze a soccer game. It is important for us to know the quantitative assessment of contribution done by a player or a team in the game as an individual or collective behavior. The mean numbers of vertices are reported to be 5–6, which is a little less than those of a perfect random system. Voronoi polygons areas can be used in evaluating the dominance of a team over the other. By introducing an excess Voronoi area, we can draw some fruitful results to appraise a player or a team rather quantitatively.

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