Effects of Steroid Treatment on Childhood ALL Stem Cells.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3462-3462
Author(s):  
Charlotte V. Cox ◽  
Paraskevi Diamanti ◽  
Pamela R. Kearns ◽  
Allison Blair
Keyword(s):  
Stem Cells ◽  
Annexin V ◽  
Absolute Number ◽  
Proliferative Potential ◽  
Disease Relapse ◽  
Childhood All ◽  
Cell Counts ◽  
Viable Cells ◽  
Significant Difference ◽  
The Absolute

Abstract Several lines of evidence indicate a central role for stem cells in the pathogenesis of human leukaemias and exemplify the need to develop strategies that target this sub-population of cells. It is proposed that these cells may exhibit different chemo-sensitivity and consequently may be resistant to drug regimens designed to kill the bulk leukaemia population. Inherently resistant leukaemia stem cells may contribute to subsequent disease relapse. Clearly, there is a need to assess the relative efficacy of therapeutic agents on the sub-populations of cells in addition to the bulk leukaemia. We have previously demonstrated that the sub-population of childhood acute lymphoblastic leukaemia (ALL) cells, capable of serially engrafting NOD/SCID mice, have a CD34+/CD19− or CD34+/CD7− phenotype in B cell precursor (BCP) ALL and T-ALL, respectively. In this investigation we have compared the efficacy of a current glucocorticoid therapeutic agent on these putative ALL stem cells with their effects on the bulk leukaemia population. The effect of dexamethasone (dex), a key component of the treatment of childhood ALL, on primary ALL cells from 13 paediatric cases was examined. Unsorted ALL cells were co-cultured with and without dex for 48 hours. Subsequently, cell viability and apoptosis were evaluated by flow cytometry using propidium iodide and annexin V staining, with Flow-Count fluorospheres to directly determine absolute cell counts. Primary cells from 11 patients with BCP ALL were sorted for expression of CD34/CD19 and cells from 2 T-ALL cases were sorted for expression of CD34/CD7. The unsorted cells and the sorted sub-fractions were co-cultured with increasing concentrations of dex (0.05 to 500 μM) to compare the relative chemosensitivity of the bulk and putative leukaemia stem cell populations. The unsorted leukaemia populations were completely refractory to dex with no significant difference in the levels of apoptosis observed or the absolute number of viable cells in the treated samples and in the untreated controls. Interestingly, when the sorted populations were assessed, an increase in the absolute numbers of viable CD34+/CD19− (1.2–9.7 fold, P<0.02) and CD34+/CD7− (2.6–5 fold, P<0.04) leukaemia cells were observed even at the highest steroid dose, compared to the respective untreated sub-fractions. The other leukaemic sub-fractions did not show a significant increase in the number of viable cells following dex exposure. These data show that 10 out of 11 drug treated primary leukaemia cells were resistant to dex. The putative CD34+/CD19− BCP ALL cells and CD34+/CD7− T-ALL cells showed a significantly enhanced proliferative potential when exposed to the drug, suggesting that it is these cells that may be responsible for disease relapse.

Role of the Transcription Factor LYL-1 in the Adult and Fetal Hematopoietic Stem Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2775-2775
Author(s):  
Claude Capron ◽  
Catherine Lacout ◽  
Yann Lecluse ◽  
Isabelle Poullion ◽  
Fedor Svinarchouk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Hematopoietic Stem Cells ◽  
Absolute Number ◽  
Binding Motif ◽  
Hematopoietic Stem ◽  
Cell Counts ◽  
Significant Difference ◽  
The Absolute

Abstract The hematopoietic stem cells (HSC) have the ability to self-renew and to give rise to all blood lineages. These processes occur via a hierarchy of progenitors with progressively more limited differentiation and self-renewal potential and are orchestrated by specialized protein such as transcription factors. LYL-1 protein contains a basic helix-loop-helix DNA binding motif also found in several proteins involved in the control of cellular proliferation and differentiation such as SCL/TAL-1. As LYL-1 shares an 80% homology at the protein level with SCL/TAL-1, we wanted to determine the function of LYL-1 in hematopoiesis and particularly on HSC. For this study, we used knock in lyl-1−/− mice in which exon 4 was replaced by LacZ/Neo cassette. Lyl−/− mice are viable and have normal blood cell counts as well as a normal marrow cellularity. In addition, using a hematopoietic colony forming cells (CFCs) assay, no significant difference was seen in the myeloid CFCs of either lyl-1−/− or lyl-1+/+ BM and FL cells except a 2-fold increase in the absolute number of BFU-E in lyl-1−/− FL as compared to lyl-1+/+ FL. We analyzed more primitive progenitors in details because using Fluorecein Di-beta Galactopyranoside (FDG)-staining assay, we showed that lyl-1 is mainly expressed in primitive Lin− Sca-1+ c-Kit+ cells (LSK) cells from either BM or FL (91 ± 7% and 78 ± 5% of FDG positive cells in lyl-1−/− BM and FL LSK cells, respectively). In addition, analysis of lyl-1−/− and lyl-1+/+ cells revealed a 1.8-fold and 2-fold decrease in the percentage of primitive LSK in BM and FL, respectively, as compared to wild type cells. Furthermore, using the Hoechst 33342 efflux assay, we noticed a significant decrease in the absolute number of more primitive LSK-SP (side population) cells in lyl-1−/− BM as compared to lyl-1+/+ BM cells (52800 ± 5412 cells/femur versus 91080 ± 8475 cells/femur, respectively) suggesting an important role of LYL-1 in the HSC function. In order to confirm this hypothesis, in vivo assays were performed. We observed a 1.5-fold decrease in the lyl-1−/− BM and FL day 12 CFU-S content as compared to lyl-1+/+ cells. Adoptive transfer experiments were subsequently performed using lethally irradiated Ly5.1 mice. Data showed that lyl-1−/− cells from either BM or FL displayed a hematopoietic reconstitution defect in competitive repopulation assays. Indeed, Ly5.1 recipients were injected with a mixture of 5x106 (5:1), 106 (1:1) or 0.5x106 (0.5:1) lyl-1−/− or lyl-1+/+ Ly5.2 expressing cells and 106 competitive BM Ly5.1 expressing cells. All hosts engrafted with lyl-1−/− BM cells shown a significant reduced levels of chimerism (% of circulating Ly5.2+ cells) as compared to hosts engrafted with lyl-1+/+ BM donors (4.3 ± 2.8% (5:1); 7.5 ± 5.5% (1:1); 0.6 ± 0.3% (0.5:1) in lyl-1−/− BM cells versus 66 ± 8% (5:1); 52 ± 9% (1:1); 53 ± 10% (0.5:1) in lyl-1+/+ BM cells) and similar difference was observed with FL donors (45 ± 2% (5:1); 25 ± 5% (1:1); 11 ± 5% (0.5:1) in lyl-1−/− FL cells versus 83 ± 1% (5:1); 70 ± 3% (1:1); 53 ± 6% (0.5:1) in lyl-1+/+ FL cells). This altered defect in HSC was also confirmed using LTC-IC in vitro experiments. Altogether, our results demonstrate an important role of the transcription factor LYL-1 on the maintenance of HSC properties.

Adipose Derived Stem Cells Characterization from Human Lipoaspirate: A Comparative Study

2013 ◽  
Vol 18 ◽  
pp. 73-83 ◽  
Author(s):  
Aris Sterodimas ◽  
Vasiliki E. Kalodimou ◽  
Beatriz Nicaretta
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Autologous Transplantation ◽  
Absolute Number ◽  
High Expression ◽  
Adipose Derived Stem Cells ◽  
Cell Counts ◽  
The Mean ◽  
The Absolute ◽  
Low Expression

Background Numerous studies have provided data on the efficacy of ADSCs, supporting their use in current and future clinical applications. This is the first study to our knowledge, which aims at comparing the cell viability and the absolute number of mesenchymal stem cells and ADSCs from three different approaches of preparing adipose tissue for autologous transplantation. Patients & MethodsAdipose tissue was taken from the hip/thigh region of 8 female donors undergoing liposuction. From every patient, there was sent three different fat samples: lipoaspirated fat decanted (A), lipoaspirated fat prepared by normal saline washing (B) and stromal enriched lipograft (C). Multi-parameter flow cytometry to determine the absolute number and viability of ADSCs was performed. ResultsThe mean absolute cell counts per gram of adipose tissue were 8.33x10⁶ in samples A and 5.97x10⁶ in sample C. In B samples the mean absolute cell counts per gram of adipose tissue were 2.13x10⁶. The presence of ADSCs specific markers in all the C samples showed high expression (> 95%) in the positive markers and low expression (< 2%) in the negative markers and are essential to validate the purity of adipose stem cells in a sample. ConclusionThe results obtained from the analysis of eight different donors of lipoaspirate indicate that the highest absolute number of viable adipose derived stem cells is found in the Stromal Enriched Lipograft (sample C). Their purity was confirmed by the high expression (> 95%) in the positive markers and low expression (< 2%) in the negative markers.

The Impact of Age and Gender on Hematopoietic Stem Cells and Immune Contexture of the Bone Marrow Microenvironment

2020 ◽  
pp. 1-6
Author(s):  
Rebar N. Mohammed
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Immune Cells ◽  
Absolute Number ◽  
Cell Number ◽  
Hematopoietic Stem ◽  
The Absolute ◽  
And Gender ◽  
The Impact

Hematopoietic stem cells (HSCs) are a rare population of cells that reside mainly in the bone marrow and are capable of generating and fulfilling the entire hematopoietic system upon differentiation. Thirty-six healthy donors, attending the HSCT center to donate their bone marrow, were categorized according to their age into child (0–12 years), adolescence (13–18 years), and adult (19–59 years) groups, and gender into male and female groups. Then, the absolute number of HSCs and mature immune cells in their harvested bone marrow was investigated. Here, we report that the absolute cell number can vary considerably based on the age of the healthy donor, and the number of both HSCs and immune cells declines with advancing age. The gender of the donor (male or female) did not have any impact on the number of the HSCs and immune cells in the bone marrow. In conclusion, since the number of HSCs plays a pivotal role in the clinical outcome of allogeneic HSC transplantations, identifying a younger donor regardless the gender is critical.

White blood cell counts in bursectomized birds

1964 ◽  
Vol 207 (6) ◽  
pp. 1371-1373 ◽  
Author(s):  
Bruce Glick ◽  
Koji Sato
Keyword(s):  
Absolute Number ◽  
Significant Decline ◽  
Bursa Of Fabricius ◽  
Optimum Level ◽  
Absolute Count ◽  
Single Intravenous Injection ◽  
Cell Counts ◽  
Circulating Lymphocytes ◽  
The Absolute ◽  
White Blood Cell Counts

Birds were bursectomized before 3 days of age. Bursectomy did not significantly influence the total or absolute number of leukocytes at 12, 19, or 41 days of age. The bursectomized birds contained significantly fewer small lymphocytes at 5 and 41 days of age than medium lymphocytes. A single intravenous injection of 4 IU or 6 USP units of ACTH/100 g body weight significantly increased the absolute heterophil counts of both sham and bursectomized birds. However, only the bursectomized birds showed a significant decline in the absolute count of lymphocytes. The results suggest that the bursa of Fabricius is necessary for an optimum level of circulating lymphocytes. The bursa may influence the lymphocyte count by releasing specific cells or elaborating a humoral factor.

scholarly journals Redox Status Is Critical for Stemness in Skin Equivalents

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hye-Ryung Choi ◽  
Youn-A Kang ◽  
Jung-Won Shin ◽  
Jung-Im Na ◽  
Chang-Hun Huh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Vitamin C ◽  
Plant Extract ◽  
Plant Extracts ◽  
Redox Status ◽  
Proliferative Potential ◽  
Basal Cells ◽  
Intense Staining ◽  
Significant Difference

The skin is constantly exposed to environmental oxidative stress. Skin equivalent (SE) models are three-dimensional systems in which cell-cell or cell-matrix interactions can be investigated. In this study, the effects of vitamin C or plant extracts with high antioxidant activities were tested. There was no significant difference in the epidermal thickness, but the basal cells became cuboidal when vitamin C or plant extracts were supplemented. Furthermore, immunohistochemical staining showed linear and intense staining ofα6 andβ1 integrin along the basement membrane in vitamin C or plant extract treated models. The p63 and PCNA were also stained. Results showed that the number of p63 and PCNA positive cells was higher in the vitamin C or plant extract treated models than in the control SEs. Although the relationship between oxidative stress and stem cells is not known, our results suggest that redox status affects the stemness and the proliferative potential of epidermal basal cells by modulating microenvironment to epidermal basal stem cells.

Enrichment of Mononuclear Cells From Cryopreserved Cord Blood Units Using the Purecell” Select System.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2159-2159
Author(s):  
Bryan P Spencer ◽  
Indreshpal Kaur ◽  
Patrick Fong ◽  
Safa Karandish ◽  
Nirmali Ponweera ◽  
...  
Keyword(s):  
T Cells ◽  
Cord Blood ◽  
Mononuclear Cells ◽  
Cellular Therapy ◽  
Standard Procedure ◽  
Absolute Number ◽  
Common Procedure ◽  
Expansion Time ◽  
Significant Difference ◽  
The Absolute

Abstract Abstract 2159 Poster Board II-136 INTRODUCTION: Many procedures for manufacturing clinical products for cellular therapy involve enrichment of mononuclear cells (MNC). The most common procedure is density gradient separation. Disadvantages of this procedure are low yield of cells especially with cryopreserved products and the open events during processing. We evaluated use of the Purecell” Select System (PALL Medical, NY) for enriching MNC from cryopreserved Cord Blood Units (CBU). METHODS: Initial experiments were performed to optimize the system for recoveries of total nucleated cells (TNC), MNC, neutrophils, lymphocytes, monocytes and CD34+/CD3+ cells. We evaluated thaw/predilute/filter vs thaw/filter, starting volumes (30 — 95mls) and three different methods for harvesting cells from the filter (standard method, input bag rinse and harvest port rinse). Once conditions were optimized, cryopreserved CBU were thawed and split into two fractions. One half of the product was diluted and processed on the Purecell” Select System. The other half was washed and ficolled. The MNC fraction was CD3+ enriched using CD3/28 beads and then cultured with rIL-2 (200 units/ml) for 14 days. The absolute number of CD3+ cells post culture, fold expansion and viabilities of these cells were determined. RESULTS: The Purecell” procedure was 15 times faster than the ficoll method. Optimal volume to load onto the filter was 50ml. When MNC were harvested by the three recommended procedures, there was no difference in recoveries of TNC, MNC, CD34+ and CD3+ cells and neutrophils. However, the lymphocyte and monocyte recoveries were higher (p<0.05 and p=0.001) when harvested with Input bag rinse compared to the standard procedure. Monocyte recoveries were also higher with the harvest port rinse (p=0.004) when compared to the standard procedure. The direct comparison studies of the two MNC enrichment systems demonstrated that the Purecell” Select System gave significantly higher recovery of TNC (p= 0.003), MNC (p=0.029), CD34+ cells (p<0.001) and granulocytes (p<0.001). There was no statistical difference in T cell recoveries, however, there was a significant difference in the recovery of T cells after CD3/28 enrichment.. Interestingly, T cells began to proliferate earlier from the PALL system compared to ficoll isolated T cells (day 4 vs day 7). Although the fold expansion was greater for the ficolled prepared cells, the absolute numbers of T cells obtained after 14 days of culture with rIL-2 was greater for the Purecell Select System in all experiments. The viabilities of the cells from both cultures were comparable. CONCLUSIONS: PALL's Purecell” Select System can be used for clinical processing since it is a functionally closed system. The advantage of this system compared to the ficoll method are the reduced time for processing, increase yield in T cells (post processing), the earlier expansion time These benefits result in an increase in absolute number of T cells in post culture. A clinical trial using this system is about to be initiated. Disclosures: Karandish: Pall Medical: Employment. McMannis:Pall Medical: Research Funding.

scholarly journals Dynamics of minor T-lymphocyte subpopulations in patients undergoing kidney transplantation

2020 ◽  
pp. 75-83
Author(s):  
S. V. Zybleva ◽  
S. L. Zyblev
Keyword(s):  
Kidney Transplantation ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Graft Function ◽  
Absolute Number ◽  
Primary Graft Dysfunction ◽  
Graft Dysfunction ◽  
Double Positive ◽  
Significant Difference ◽  
The Absolute

Objective: to study the dynamics of the indicators of CD3+CD4+CD8+double-positive and CD3+CD4-CD8- double-negative T-lymphocytes in patients who underwent kidney transplantation. Material and methods. Three groups were formed out of 197 allograft recipients. PGF group consisted of patients with primary satisfactory graft function. PGD group - with primary graft dysfunction. GR group - with primary graft dysfunction and histologically confirmed graft rejection. We studied the CD3+CD4+CD8+ (DP) and CD3+CD4-CD8- (DN) T-lymphocyte levels before the transplantation, and on the 1st, 3rd, 7th, 30th, and 90th days after the transplantation. Results. Within a month after the transplantation we noted a decrease in the relative DN T-lymphocyte level in the PGF group, while in the PGD and GR groups this indicator significantly increased. By the 90th day, the count of DN T-lymphocytes had remained unchanged in the PGD group, while there had been a statistically significant increase of this subpopulation in the PGF group. The absolute counts of DN T-lymphocytes in the PGF group on the 1st and 7th days were lower than in the GR group. On the 90th day, there was no statistically significant difference in the absolute number of DN T-lymphocytes in the recipient groups. In all the groups, there was a decrease in the number of DP T-lymphocytes on the 1st day, however, in the PGF group, the relative level was significantly higher. This tendency retained for 3 months. There were no statistically significant differences between the PGD and GR groups. The absolute number of DP T-lymphocytes in the PGF group during the entire observation period was significantly higher than in the PGD and GR groups. Conclusion. We noted a decrease in the indicators of DN T-lymphocytes in the PGF group associated with the increase in the DP T-lymphocyte level within the first three months. In the PGD and GR groups, an increase in the DN T-lymphocyte level was revealed due to a decrease in the indicators of DP T-lymphocytes within 90 days after transplantation.

scholarly journals Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla

2018 ◽  
Vol 45 (2) ◽  
pp. 31-35 ◽  
Author(s):  
Kr. Hristov ◽  
N. Gateva ◽  
P. Stanimirov ◽  
N. Ishkitiev ◽  
R. Tsikandelova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Citric Acid ◽  
Sodium Hypochlorite ◽  
Colorimetric Assay ◽  
Permanent Teeth ◽  
Clinical Challenge ◽  
Viable Cells ◽  
Chemical Agents ◽  
Significant Difference ◽  
Apical Papilla

Abstract The endodontic treatment of immature permanent teeth with necrotic pulp is a serious clinical challenge. The chemical agents, used in regenerative procedures, should be selected not only based on their bactericidal/bacteriostatic properties, but also on their ability to ensure the survival of the patient’s stem cells. The aim of this study was to evaluate the effect of citric acid on the vitality of SCAP in a model of an immature tooth root. Models of immature roots were created from 12 freshly extracted teeth. The models were gas sterilized with ethylene oxide and they were separated into three groups, based on the used combinations of irrigants: 1) 1.5% sodium hypochlorite / 17% EDTA; 2) 1.5% sodium hypochlorite / 10% citric acid; 3) saline. SCAPs in a hyaluronic acid–based scaffold were seeded into the canals and cultured for 7 days. Viable cells were quantified using a colorimetric assay. There was no statistically significant difference between the groups, irrigated with NaOCl/EDTA and NaOCl/citric acid. The results from our experiment show that 10% citric acid can be used in combination with 1.5% NaOCl in a regenerative endodontic procedure.

scholarly journals In vivo administration of stem cell factor to mice increases the absolute number of pluripotent hematopoietic stem cells

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 445-455 ◽  
Author(s):  
DM Bodine ◽  
NE Seidel ◽  
KM Zsebo ◽  
D Orlic
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Stem Cell ◽  
Peripheral Blood ◽  
Stem Cell Factor ◽  
Absolute Number ◽  
Hematopoietic Stem ◽  
The Absolute ◽  
In Vivo Administration

We have examined the effects of administration of stem cell-factor (SCF) on the number and distribution of pluripotent hematopoietic stem cells (PHSC) in normal mice. Using the competitive repopulation assay we found that in vivo administration of SCF increases the absolute number of PHSC per mouse threefold. The increased numbers of PHSC are found in the peripheral blood and spleen of the SCF-treated animals. The spleen and peripheral blood stem cells completely repopulated the erythroid, myeloid, and lymphoid lineages of irradiated or W/Wv hosts, similar to bone marrow PHSC. PHSC from the peripheral blood of SCF- treated mice have a lineage marker-negative, c-kit-positive phenotype that is indistinguishable from that of bone marrow PHSC. The increase in the absolute number of spleen PHSC is associated with efficient gene transfer to these cells without prior treatment with 5-fluorouracil. This is a US government work. There are no restrictions on its use.

scholarly journals In vivo administration of recombinant methionyl human stem cell factor expands the number of human marrow hematopoietic stem cells

Blood ◽  
1993 ◽  
Vol 82 (3) ◽  
pp. 784-791 ◽  
Author(s):  
J Tong ◽  
MS Gordon ◽  
EF Srour ◽  
RJ Cooper ◽  
A Orazi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Locally Advanced ◽  
Proliferative Potential ◽  
Human Stem Cell ◽  
Hematopoietic Stem ◽  
Hla Dr ◽  
The Absolute ◽  
Human Stem Cell Factor

A growing number of in vitro studies suggest that recombinant human stem cell factor (SCF) is capable of augmenting the proliferative capacity of human hematopoietic progenitor cells (HPC) and stem cells (HSC). We further evaluated this biologic effect by analyzing the response of bone marrow (BM) HPCs and HSCs to the administration of SCF in eight patients with locally advanced or metastatic breast cancer who were enrolled in an ongoing phase I study. SCF was administered for 14 days by daily subcutaneous injection at dosages of 10, 25, or 50 micrograms/kg/d. BM CD34+ HLA-DR+ and CD34+ HLA-DR- CD15- cells, previously shown by our laboratory to be enriched for various classes of differentiated and primitive HPCs, respectively, were quantitated in BM samples on day 0 (pretreatment) and day 15 (posttreatment). These CD34+ HLA-DR+ and CD34+ HLA-DR- CD15- cells were then isolated by cell- sorting and assayed for several classes of HPCs, including the high-- proliferative potential colony-forming cell (HPP-CFC), the burst- forming unit--megakaryocyte (BFU-MK), and the long-term BM culture-- initiating cell (LTBMC-IC). SCF administration resulted in a 3.3-fold (range, 1.4- to 18.8-fold; P = .018) increase in the absolute numbers of CD34+ cells, a 3.7-fold (range, 1.2- to 8.2-fold; P = .028) increase in the absolute numbers of CD34+ HLA-DR+ cells, and a 2.4-fold (range, 1.1- to 29.3-fold; P = .010) increase in the absolute numbers of CD34+ HLA-DR- CD15- cells. Following the infusion of SCF, a statistically significant increase in the absolute numbers of HPP-CFC (P = .018), BFU- MK (P = .046), CFU-granulocyte, erythrocyte, monocyte, megakaryocyte (CFU-GEMM: P = .043), BFU-erythrocyte (BFU-E; P = .043), CFU- granulocyte, macrophage (CFU-GM; P = .045), and CFU-megakaryocyte (CFU- MK; P = .028) per milliliter of marrow was observed. Stromal cell-free LTBMCs supplemented with SCF and interleukin-3 (IL-3), initiated with CD34+ HLA-DR- CD15- cells obtained on day 0, produced viable cells for 9.6 weeks, compared with 11.5 weeks for LTBMCs initiated with CD34+ HLA- DR- CD15- cells obtained on day 15. Cumulative cellular production by LTBMCs initiated with day 15 CD34+ HLA-DR- CD15- cells was statistically greater than that by day 0 LTBMCs (P = .031). These same cultures produced CFU-GM for 6.3 weeks (day 0) versus 9 weeks (day 15).(ABSTRACT TRUNCATED AT 400 WORDS)

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