scholarly journals A Rapid Method for Estimation of the Total Leukocyte Count

Blood ◽  
1958 ◽  
Vol 13 (7) ◽  
pp. 677-683 ◽  
Author(s):  
NAOMI R. BENJAMIN
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Rapid Method ◽  
Leukocyte Count ◽  
Screening Method ◽  
Total Leukocyte Count ◽  
Blood Cell Count ◽  
Microscope Slide ◽  
Standard Size ◽  
Microscopic Appearance

Abstract A technic has been devised for the estimation of the white blood cell count by examination of a stained drop of blood of standard size. The drop is measured by immersing a pinhead in the blood and then touching onto a microscope slide the blood which adheres to the surface of the pinhead. The drop is dried and stained and the count is estimated by comparing its microscopic appearance with photographs or "standard" preparations. It was developed to be used as a screening procedure in case of atomic war, but it may prove useful in epidemics or as a routine screening method in the hematology laboratories of large hospitals.

scholarly journals Efek Merokok Berat terhadap Jumlah Leukosit dan Jenis Leukosit pada Pria Usia Produktif di Kelurahan Tanjung Pinang Kota Palangka Raya

2019 ◽  
Vol 1 (2) ◽  
pp. 34-40
Author(s):  
Rinny Ardina ◽  
Nada Soraya
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
Leukocyte Count ◽  
Sampling Technique ◽  
Blood Cell Count ◽  
Differential Leukocyte Count ◽  
Cross Sectional ◽  
Heavy Smoking

Prevalence of productive men smokers in Kalimantan Tengah in 2013 has been reached 64.9% with a mean of smoking about 12.3 cigarettes a day. Cigarette smoke contains 1015 of oxidative free radicals which can cause oxidative stress. It will trigger of activation of inflammatory genes and increase of cytokines release by increasing of leukocytes and activate some of the differential leukocytes. Continual inflammation can cause atherosclerosis and coronary heart disease. This study aimed to determine the effects of heavy smoking on white blood cell count and differential leukocyte count in productive men in Kelurahan Tanjung Pinang, Palangka Raya. This study used a descriptive observational method with a cross-sectional design. The sample obtained by 28 people with purposive sampling technique with criteria: men, active smoker, smoking more than ten years, working as a farmer, fish farmer, or laborer, and willing to become a respondent. White blood cell count and differential leukocyte count were analyzed by an automatic method using Hematology Analyzer. The results showed that 3.6% of active smokers had high white blood cell count and others had low white blood cell count (3.6% ). Whereas differential leukocyte count showed that 2 persons had high of eosinophils (7.10%), 5 people had low of eosinophils (17.9%), one person had little of neutrophils (3.6%), 2 persons had high of lymphocytes (7.20%), 10 people had little of lymphocytes (35.7%), and 15 people had high of monocytes (53.6%). To reduce the risks, preventive and educative actions from medical personnel are needed for active smokers and their families.

scholarly journals Biochemical and hematological parameters in chronic kidney disease

2018 ◽  
Vol 4 (1) ◽  
pp. 4-11
Author(s):  
S. Singh ◽  
S. Bhatta
Keyword(s):  
Chronic Kidney Disease ◽  
Platelet Count ◽  
Kidney Disease ◽  
Blood Cell ◽  
Biochemical Parameters ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Blood Cell Count ◽  
Red Blood Cell Count

Background: Chronic kidney disease is a worldwide public health problem. It is associated with various biochemical and hematological abnormalities that leads to morbidity and mortality. The aim of this study was to evaluate the biochemical and hematological parameters in chronic kidney disease patients.Methods: This is a prospective cross sectional study conducted over a period of six months on 52 chronic kidney disease patients at Kist Medical College Teaching Hospital, Lalitpur, Nepal. Biochemical parameters such as urea, creatinine, calcium, phosphorous, sodium, potassium and hematological parameters like hemoglobin, hematocrit, red blood cell count, total leukocyte count, platelet count were measured using standard techniques in chronic kidney disease cases and the findings were compared with age and sex matched controls. Results were analyzed using SPSS 21 for Windows.Results: Hemoglobin, hematocrit, red blood cell count, total leukocyte count and platelet count were reduced and statistically significant in chronic kidney disease patients compared to controls(p <0.05).In biochemical parameters, serum urea, creatinine, phosphorous were increased and calcium was reduced which was found statistically significant as compared to controls (p <0.05). The combination of diabetes and hypertension was the lead cause of chronic kidney disease found in 38.46% followed by hypertension alone in 30.76%Conclusion: Biochemical and hematological parameters are deranged in patients with chronic kidney disease. Routine evaluation of these parameters is useful in the management of these patients.

scholarly journals LEUKOSITOSIS BER-FLAGGING BINTANG () BERPOTENSI ADANYA INTERFERENSI ALAT ANALISIS HEMATOLOGI OTOMATIS

2018 ◽  
Vol 17 (2) ◽  
pp. 121
Author(s):  
Christine Sugiarto ◽  
Leni Lismayanti ◽  
Nadjwa Zamalek Dalimoenthe
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell Count ◽  
Ammonium Salt ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Leukocyte Count ◽  
Peripheral Blood Smear ◽  
Impedance Method ◽  
Blood Cell Count

Leukocytosis is a condition in which there is an increasing number of white blood cell count in the peripheral blood compared to thenormal range based on age. Several conditions can amplify leukocyte count from haematological auto analyzers, not only those whichcorrespond to the pathologic and physiologic condition, but also with other factors, such as diluent and haematological auto analyzer’smethods. The information about these factors should be evaluated to lessen errors in the patient’s diagnosis and therapy. This casereport describes a leukocytosis in a 35-day old baby boy, diagnosed as duodenal obstruction, admitted in Paediatric Surgery Department,Hasan Sadikin Hospital, Bandung. Discrepancies occurred in this patient’s leukocyte count with some different haematological autoanalyzers. The leukocyte count from the auto analyzer by impedance method and ammonium salt diluent was 129.200/mm3 which wasindicated by a star-flagged (), while from the auto analyzer with light scatter method and anhydrous sodium sulphate and sodiumchloride diluent was 9.200/mm3, from manual count by the counting chamber with Turk diluent was 14.200/mm3 and the estimationby peripheral blood smear was 7.000–10.000/mm3. False leukocytosis by auto analyzer with impedance method was caused by thelimitation of the analyzer’s method and by the erythrocyte lysine reagent (diluent) using ammonium salt. As investigated in this case,the interferences were thought as being caused by the Lyses-resistant Red Blood Cells, thus the non-lysed/lyses cells which were enlargedin size were identified as leukocytes other than erythrocytes. It can be that the white blood concluded cell count examination which isindicated by star-flagged (), or white blood cell count >100.000/mm3 must be confirmed by manual examination (counting chamberand peripheral blood smear) or by another haematological auto analyzer method that has a different and more potent diluent

Changes in Automated Complete Blood Cell Count and Differential Leukocyte Count Results Induced by Storage of Blood at Room Temperature

2002 ◽  
Vol 126 (3) ◽  
pp. 336-342
Author(s):  
Gene L. Gulati ◽  
Lawrence J. Hyland ◽  
William Kocher ◽  
Rolland Schwarting
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Room Temperature ◽  
Leukocyte Count ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Normal Range ◽  
Differential Leukocyte Count ◽  
The Mean ◽  
Blood Specimens

Abstract Objective.—To delineate changes that occur in various parameters of automated complete blood cell count (CBC) and differential leukocyte count (differential) on prolonged storage of blood at room temperature. Design.—A CBC and an automated differential were performed on the Coulter Gen.S on 40 K3 (tripotassium ethylenediamine-tetraacetate) EDTA-anticoagulated blood specimens once daily, specimen volume permitting, for 3 to 7 days. Specimens were kept at room temperature throughout the study. The results were tabulated using a personal computer with Excel software. Percent change or absolute difference from the initial value for each parameter for each subsequent day of the study period was calculated. Results.—Among the CBC parameters, hemoglobin, red blood cell count, and mean corpuscular hemoglobin were stable for the duration of the study (7 days), white blood cell count was stable for at least 3 days (up to 7 days, if the count was within or above the normal range), and platelet count was stable for at least 4 days (up to 7 days, if the count was within or above the normal range). The mean corpuscular volume, mean platelet volume, hematocrit, and red blood cell distribution width each increased, and the mean corpuscular hemoglobin concentration decreased from day 2 onward. Among the differential parameters, the relative percentages and absolute numbers of neutrophils, lymphocytes, and eosinophils tended to increase, whereas those of monocytes trended downward over time. Limited data on basophils did not reveal an appreciable change. Conclusion.—Blood specimens stored at room temperature for more than 1 day (up to 3 days or possibly longer) were found to be acceptable with some limitations for CBC but not for the differential.

Cigarette Smoking Increases Thromboxane A2 Formation without Affecting Platelet Survival in Young Healthy Females

1992 ◽  
Vol 68 (05) ◽  
pp. 583-588 ◽  
Author(s):  
Annika Dotevall ◽  
Christina Rångemark ◽  
Elsa Eriksson ◽  
Jack Kutti ◽  
Hans Wadenvik ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cigarette Smoking ◽  
Blood Cell ◽  
Urinary Excretion ◽  
Life Span ◽  
Cell Count ◽  
Thromboxane A2 ◽  
Blood Cell Count ◽  
Platelet Activity ◽  
Pai 1

SummarySmoking is a risk factor for the development of atherosclerotic cardiovascular disease, in men as well as in women. An increased urinary excretion of the thromboxane metabolite 2,3-dinor-thromboxane B2 (Tx-M) has been observed in smokers of both genders, suggesting that cigarette smoking may facilitate cardiovascular disease via an action on the platelets. The present study addressed the hypothesis that the increased Tx-M excretion in female smokers reflects a true facilitation of platelet reactivity in vivo, rather than an increased destruction of the platelets. In healthy female volunteers (aged 20–46 years, 18 smokers and 17 non-smokers) platelet life-span and indices of platelet activity were determined, together with plasma levels of plasminogen activator inhibitor-1 (PAI-1), fibrinogen, peripheral blood cell counts and hematocrit. The urinary excretion of Tx-M was higher in smokers than in non-smokers (361 vs. 204 pg/mg creatinine, respectively, p <0.05), while plasma and urinary β-thromboglobulin, plasma platelet factor 4, platelet mean life-span and platelet production rate did not differ between the groups. PAI-1 activity, white blood cell count and hematocrit were higher in smokers than in non-smokers (p <0.05). These data indicate that smoking facilitates platelet formation of thromboxane A2 without affecting platelet survival; i.e. it increases the activity of platelets without affecting their viability to a measurable extent. Such an increase in platelet activity, operating in parallel to a reduced fibrinolytic activity and a higher hematocrit and white blood cell count, may play an etiological role in smoking-induced cardiovascular disease in women.

Influence of increased doses of fenbendazole supramolecular complex on sheep’s organism

2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Negative Influence ◽  
Supramolecular Complex ◽  
Control Group ◽  
Blood Cell Count ◽  
Russian Scientific ◽  
Russian Scientific Research Institute ◽  
Hematological And Biochemical Parameters

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.

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