scholarly journals Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

BMC Genomics ◽  
2021 ◽  
Vol 22 (S3) ◽  
Author(s):  
Menglei Wang ◽  
Chenhui Yang ◽  
Kangning Wei ◽  
Miao Zhao ◽  
Liqiang Shen ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Floral Development ◽  
Mitochondrial Gene ◽  
Growth Conditions ◽  
Winter Dormancy ◽  
Temporal Expression ◽  
Expression Study ◽  
Leaf Stage ◽  
Jointing Stage ◽  
Post Transcriptional Regulation

Abstract Background Winter wheat requires prolonged exposure to low temperature to initiate flowering (vernalization). Shoot apical meristem of the crown is the site of cold perception, which produces leaf primordia during vegetative growth before developing into floral primordia at the initiation of the reproductive phase. Although many essential genes for winter wheat cold acclimation and floral initiation have been revealed, the importance of microRNA (miRNA) meditated post-transcriptional regulation in crowns is not well understood. To understand the potential roles of miRNAs in crown tissues, we performed a temporal expression study of miRNAs in crown tissues at the three-leaf stage, winter dormancy stage, spring green-up stage, and jointing stage of winter wheat grown under natural growth conditions. Results In total, 348 miRNAs belonging to 298 miRNA families, were identified in wheat crown tissues. Among them, 92 differentially expressed miRNAs (DEMs) were found to be significantly regulated from the three-leaf stage to the jointing stage. Most of these DEMs were highly expressed at the three-leaf stage and winter dormancy stage, and then declined in later stages. Six DEMs, including miR156a-5p were markedly induced during the winter dormancy stage. Eleven DEMs, including miR159a.1, miR390a-5p, miR393-5p, miR160a-5p, and miR1436, were highly expressed at the green-up stage. Twelve DEMs, such as miR172a-5p, miR394a, miR319b-3p, and miR9676-5p were highly induced at the jointing stage. Moreover, 14 novel target genes of nine wheat or Pooideae-specific miRNAs were verified using RLM-5′ RACE assay. Notably, six mTERFs and two Rf1 genes, which are associated with mitochondrial gene expression, were confirmed as targets of three wheat-specific miRNAs. Conclusions The present study not only confirmed the known miRNAs associated with phase transition and floral development, but also identified a number of wheat or Pooideae-specific miRNAs critical for winter wheat cold acclimation and floral development. Most importantly, this study provided experimental evidence that miRNA could regulate mitochondrial gene expression by targeting mTERF and Rf1 genes. Our study provides valuable information for further exploration of the mechanism of miRNA mediated post-transcriptional regulation during winter wheat vernalization and inflorescent initiation.

scholarly journals Naturally Occurring Ecdysteroids in Triticum aestivum L. and Evaluation of Fenarimol as a Potential Inhibitor of Their Biosynthesis in Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2855
Author(s):  
Anna Janeczko ◽  
Jana Oklestkova ◽  
Danuše Tarkowská ◽  
Barbara Drygaś
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Animal Kingdom ◽  
Experimental Conditions ◽  
P450 Monooxygenase ◽  
Naturally Occurring ◽  
Regulatory Effects ◽  
Potential Use

Ecdysteroids (ECs) are steroid hormones originally found in the animal kingdom where they function as insect molting hormones. Interestingly, a relatively high number of these substances can also be formed in plant cells. Moreover, ECs have certain regulatory effects on plant physiology, but their role in plants still requires further study. One of the main aims of the present study was to verify a hypothesis that fenarimol, an inhibitor of the biosynthesis of ECs in the animal kingdom, also affects the content of endogenous ECs in plants using winter wheat Triticum aestivum L. as a model plant. The levels of endogenous ECs in winter wheat, including the estimation of their changes during a course of different temperature treatments, have been determined using a sensitive analytical method based on UHPLC-MS/MS. Under our experimental conditions, four substances of EC character were detected in the tissue of interest in amounts ranging from less than 1 to over 200 pg·g−1 FW: 20-hydroxyecdysone, polypodine B, turkesterone, and isovitexirone. Among them, turkesterone was observed to be the most abundant EC and accumulated mainly in the crowns and leaves of wheat. Importantly, the level of ECs was observed to be dependent on the age of the plants, as well as on growth conditions (especially temperature). Fenarimol, an inhibitor of a cytochrome P450 monooxygenase, was shown to significantly decrease the level of naturally occurring ECs in experimental plants, which may indicate its potential use in studies related to the biosynthesis and physiological function of these substances in plants.

Application of natural and synthetic polyamines as growth regulators to improve the freezing tolerance of winter wheat (Triticum aestivum L.)

2012 ◽  
Vol 60 (1) ◽  
pp. 1-10 ◽  
Author(s):  
D. Todorova ◽  
I. Sergiev ◽  
V. Alexieva
Keyword(s):  
Winter Wheat ◽  
Foliar Application ◽  
Normal Growth ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Freezing Stress ◽  
Preliminary Treatment ◽  
Fresh Weight ◽  
Soil Culture ◽  
Weight Losses

Wheat cultivars were grown as soil culture under normal growth conditions. Twoweek- old seedlings were exposed to 4°C for 6 h and then transferred to −12°C for 24 h in the dark. Twenty-four hours before freezing stress, some of the plants were sprayed with aqueous solutions of spermine, spermidine, putrescine, 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA). The data showed that freezing stress caused a decrease in the fresh weight, chlorophyll content and plant survival rate, accompanied by a simultaneous accumulation of free proline and the enhanced leakage of electrolytes. Preliminary treatment with polyamines caused a decline in electrolyte leakage and a considerable augmentation in proline quantity, indicating that the compounds are capable of preventing frost injury. Additionally, the foliar application of polyamines retarded the destruction of chlorophyll, and lessened fresh weight losses due to freezing stress. The synthetic triamine DETA was the most effective, having the most pronounced action in all the experiments, followed by the tetraamine spermine. The application of polyamines to wheat crops could be a promising approach for improving plant growth under unfavourable growth conditions, including freezing temperatures. The results demonstrate that treatment with polyamines could protect winter wheat by reducing the stress injuries caused by subzero temperatures.

scholarly journals Mamo decodes hierarchical temporal gradients into terminal neuronal fate

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Ling-Yu Liu ◽  
Xi Long ◽  
Ching-Po Yang ◽  
Rosa L Miyares ◽  
Ken Sugino ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Neuronal Diversity ◽  
Temporal Expression ◽  
Identity Maintenance ◽  
Temporal Gene Expression ◽  
Neuronal Fate ◽  
Mammalian Genes ◽  
Post Transcriptional Regulation

Temporal patterning is a seminal method of expanding neuronal diversity. Here we unravel a mechanism decoding neural stem cell temporal gene expression and transforming it into discrete neuronal fates. This mechanism is characterized by hierarchical gene expression. First, Drosophila neuroblasts express opposing temporal gradients of RNA-binding proteins, Imp and Syp. These proteins promote or inhibit chinmo translation, yielding a descending neuronal gradient. Together, first and second-layer temporal factors define a temporal expression window of BTB-zinc finger nuclear protein, Mamo. The precise temporal induction of Mamo is achieved via both transcriptional and post-transcriptional regulation. Finally, Mamo is essential for the temporally defined, terminal identity of α’/β’ mushroom body neurons and identity maintenance. We describe a straightforward paradigm of temporal fate specification where diverse neuronal fates are defined via integrating multiple layers of gene regulation. The neurodevelopmental roles of orthologous/related mammalian genes suggest a fundamental conservation of this mechanism in brain development.

Temperature and leaf area expansion of sugarcane: integration of controlled-environment, field and model studies

1998 ◽  
Vol 25 (7) ◽  
pp. 819 ◽  
Author(s):  
Michael J. Robertson ◽  
Graham D. Bonnett ◽  
R. Michael Hughes ◽  
Russell C. Muchow ◽  
James A. Campbell
Keyword(s):  
Leaf Area ◽  
Leaf Senescence ◽  
Leaf Size ◽  
Field Studies ◽  
Growth Conditions ◽  
Thermal Time ◽  
Leaf Position ◽  
Potential Growth ◽  
Leaf Stage ◽  
Leaf Appearance

Canopy development is an important determinant of crop radiation interception, and in the absence of stress is mainly driven by temperature. The responses to temperature of the component processes of canopy dynamics in sugarcane: leaf appearance, leaf size, tillering, and leaf senescence, were analysed for the commercial Australian cultivar, Q117. Data were derived under optimal growth conditions from controlled environments, and from irrigated field studies in subtropical and tropical locations. Regression of number of fully-expanded leaves in field-grown plants against cumulative thermal time revealed that the thermal time between the appearance of successive leaves increased as a function of leaf number, such that leaf 1 required 86˚Cd and leaf 40 required 160˚Cd. At any moment, on average there were 3.7 leaves still expanding on the stalks. Functions describing leaf appearance gave acceptable prediction of the time course of leaf appearance taken from independent datasets of field-grown plant and ratoon crops. Leaf size increased with leaf position, with the largest leaves observed at approximately leaf 17 and above. Combining functions describing leaf appearance and leaf size as a function of leaf position allowed estimation of leaf area index (LAI) of main stems in plant and ratoon crops in subtropical and tropical environments. Tiller LAI, derived by difference, accounted for 60–90% of total LAI at the 5- leaf stage, declining to 20–50% at the 15-leaf stage. Plant and ratoon crops were similar in terms of the amount and proportion of tiller LAI. Combining data from all field studies indicated under potential growth conditions, leaf senescence was closely related to leaf production. The functions derived in this study give a basis for simulating canopy dynamics under potential growth conditions in sugarcane, though the extent of genotypic variation for the key parameters and their modification by stress remains to be assessed.

scholarly journals Affordable Phenotyping of Winter Wheat under Field and Controlled Conditions for Drought Tolerance

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 882
Author(s):  
Dhananjay Kumar ◽  
Sandeep Kushwaha ◽  
Chiara Delvento ◽  
Žilvinas Liatukas ◽  
Vivekanand Vivekanand ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Winter Wheat ◽  
Growth Rates ◽  
Plant Biomass ◽  
Growth Conditions ◽  
Seedling Stage ◽  
Growth Stages ◽  
Digital Cameras ◽  
Relative Growth ◽  
Relative Growth Rates

Drought stress is one of the key plant stresses reducing grain yield in cereal crops worldwide. Although it is not a breeding target in Northern Europe, the changing climate and the drought of 2018 have increased its significance in the region. A key challenge, therefore, is to identify novel germplasm with higher drought tolerance, a task that will require continuous characterization of a large number of genotypes. The aim of this work was to assess if phenotyping systems with low-cost consumer-grade digital cameras can be used to characterize germplasm for drought tolerance. To achieve this goal, we built a proximal phenotyping cart mounted with digital cameras and evaluated it by characterizing 142 winter wheat genotypes for drought tolerance under field conditions. The same genotypes were additionally characterized for seedling stage traits by imaging under controlled growth conditions. The analysis revealed that under field conditions, plant biomass, relative growth rates, and Normalized Difference Vegetation Index (NDVI) from different growth stages estimated by imaging were significantly correlated to drought tolerance. Under controlled growth conditions, root count at the seedling stage evaluated by imaging was significantly correlated to adult plant drought tolerance observed in the field. Random forest models were trained by integrating measurements from field and controlled conditions and revealed that plant biomass and relative growth rates at key plant growth stages are important predictors of drought tolerance. Thus, based on the results, it can be concluded that the consumer-grade cameras can be key components of affordable automated phenotyping systems to accelerate pre-breeding for drought tolerance.

Winter Wheat (Triticum aestivum) Growth Stage Effect on Paraquat Bioactivity

1991 ◽  
Vol 5 (2) ◽  
pp. 439-441
Author(s):  
Randy L. Anderson ◽  
David C. Nielsen
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Growth Stage ◽  
Time Of Day ◽  
Growth Stages ◽  
Leaf Stage ◽  
Time Of Application ◽  
Biomass Reduction

Paraquat was applied at 0.28 and 0.56 kg ai ha-1to winter wheat at five growth stages at 0800, 1300, and 1600 hr to determine whether growth stage or time of application influenced winter wheat response to paraquat. Paraquat bioactivity was affected by growth stage. Biomass reduction by paraquat was 84% when winter wheat was in the 1 to 3 leaf stage, but only 68% when application was delayed until tillering. Paraquat bioactivity continued to decrease at later growth stages. The time of day when paraquat was applied did not affect its bioactivity on winter wheat.

scholarly journals Systematic Analysis of FASTK Gene Family Alterations in Cancer

2021 ◽  
Vol 22 (21) ◽  
pp. 11337
Author(s):  
Lorena Magraner-Pardo ◽  
Dino Gobelli ◽  
Miguel A. de la Fuente ◽  
Tirso Pons ◽  
María Simarro
Keyword(s):  
Mitochondrial Gene ◽  
Interaction Network ◽  
Genetic Alterations ◽  
Future Research ◽  
Mrna Levels ◽  
Systematic Analysis ◽  
Lung Cancers ◽  
Protein Protein Interaction ◽  
Starting Point ◽  
Post Transcriptional Regulation

The FASTK family of proteins have been recently reported to play a key role in the post-transcriptional regulation of mitochondrial gene expression, including mRNA stability and translation. Accumulated studies have provided evidence that the expression of some FASTK genes is altered in certain types of cancer, in agreement with the central role of mitochondria in cancer development. Here, we obtained a pan-cancer overview of the genomic and transcriptomic alterations of FASTK genes. FASTK, FASTKD1, FASTKD3 and FASTKD5 showed the highest rates of genetic alterations. FASTK and FASTKD3 alterations consisted mainly of amplifications that were seen in more than 8% of ovarian and lung cancers, respectively. FASTKD1 and FASTKD5 were the most frequently mutated FASTK genes, and the mutations were identified in 5–7% of uterine cancers, as well as in 4% of melanomas. Our results also showed that the mRNA levels of all FASTK members were strongly upregulated in esophageal, stomach, liver and lung cancers. Finally, the protein-protein interaction network for FASTK proteins uncovers the interaction of FASTK, FASTKD2, FASTKD4 and FASTKD5 with cancer signaling pathways. These results serve as a starting point for future research into the potential of the FASTK family members as diagnostic and therapeutic targets for certain types of cancer.

scholarly journals Evaluation of Crop Coefficient, Cumulative and Dynamic Evapo-Transpiration of Winter Wheat under Deficit Irrigation Treatments in Weighing Lysimeter in Beijing, China

2017 ◽  
Vol 1 (1) ◽  
pp. 38 ◽  
Author(s):  
Mohamad Hesam Shahrajabian ◽  
Ali Soleymani ◽  
Peter Oko Ogbaji ◽  
Xuzhang Xue
Keyword(s):  
Winter Wheat ◽  
Precision Agriculture ◽  
Growth Yield ◽  
Crop Coefficient ◽  
Weather Data ◽  
Flowering Stage ◽  
Daily Weather Data ◽  
Irrigation Treatments ◽  
Jointing Stage ◽  
Weighing Lysimeter

<p><em>The availability of water plays an important role in plant growth, yield and quality of crops In 2012-2013 experiment, Irrigation treatments were (1): Irrigation before sowing (60 Liter), (2): Irrigation before sowing (30 Liter) + before freezing (30 Liter); (3): before sowing (30 Liter) + before freezing (30 Liter) + in the beginning of erecting stage (60 Liter) + at flowering stage (60 Liter); (4): before sowing (30 Liter) + before freezing (30 Liter) + at booting stage (60 Liter) + at flowering stage (60 Liter). The weighing lysimeter system is located in National Precision Agriculture Demonstration Station in Xiaotangshang Town of Beijing. The maximum evapotranspiration value in March, April and May was obtained for lysimeter 10 (I2) (558.70 kg), lysimeter 11 (I3) (467.25 kg), and lysimeter 10 (I2) (488.68 kg), respectively. Knowledge about changes of soil water changes during successive growing seasons from planting to final ripening is a necessary tool for an effective planning of irrigation programs, in order to improve both quality and quantity of crop. From October to June, evapo-transpiration trends increased steadily, especially in last four months, in which the lysimeter fields were covered by winter wheat completely. In 2013-2014 experiment, Irrigation treatments were (1) no irrigantion, (2) irrigation only at jointing stage (60L), (3) irrigation at jointing (60L) and flowering stage (60L), (4) at jointing stage (April 8th, 60L), 100% flowering stage (April 30th, 60L), and grain filling period (May 10th, 60L) before irrigation, crop coefficient (KC) was low and exactly after irrigation, it increased and then it decreased gradually day by day. R2 between evapotranspiration which is on the basis of hourly weather data and daily weather data with measured ET in big lysimter was 0.962 and 0.953, respectively. Evapotranspiration and crop coefficient almost exactly match according to R2 of the regression. In conclusion, evapotranspiration and KC give a closer idea of the value of research on relationship between evapotranspiration and crop coefficient.</em></p>

Sign in / Sign up

Export Citation Format

Share Document