scholarly journals A buffered media system for yeast batch culture growth

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rianne C. Prins ◽  
Sonja Billerbeck
Keyword(s):  
Nitrogen Source ◽  
Ammonium Sulfate ◽  
Batch Culture ◽  
Biomass Production ◽  
Buffering Capacity ◽  
Drop Out ◽  
Batch Cultures ◽  
Media System ◽  
The Media ◽  
Media Types

Abstract Background Fungi are premier hosts for the high-yield secretion of proteins for biomedical and industrial applications. The stability and activity of these secreted proteins is often dependent on the culture pH. As yeast acidifies the commonly used synthetic complete drop-out (SD) media that contains ammonium sulfate, the pH of the media needs to be buffered in order to maintain a desired extracellular pH during biomass production. At the same time, many buffering agents affect growth at the concentrations needed to support a stable pH. Although the standard for biotechnological research and development is shaken batch cultures or microtiter plate cultures that cannot be easily automatically pH-adjusted during growth, there is no comparative study that evaluates the buffering capacity and growth effects of different media types across pH-values in order to develop a pH-stable batch culture system. Results We systematically test the buffering capacity and growth effects of a citrate-phosphate buffer (CPB) from acidic to neutral pH across different media types. These media types differ in their nitrogen source (ammonium sulfate, urea or both). We find that the widely used synthetic drop-out media that uses ammonium sulfate as nitrogen source can only be effectively buffered at buffer concentrations that also affect growth. At lower concentrations, yeast biomass production still acidifies the media. When replacing the ammonium sulfate with urea, the media alkalizes. We then develop a medium combining ammonium sulfate and urea which can be buffered at low CPB concentrations that do not affect growth. In addition, we show that a buffer based on Tris/HCl is not effective in maintaining any of our media types at neutral pH even at relatively high concentrations. Conclusion Here we show that the buffering of yeast batch cultures is not straight-forward and addition of a buffering agent to set a desired starting pH does not guarantee pH-maintenance during growth. In response, we present a buffered media system based on an ammonium sulfate/urea medium that enables relatively stable pH-maintenance across a wide pH-range without affecting growth. This buffering system is useful for protein-secretion-screenings, antifungal activity assays, as well as for other pH-dependent basic biology or biotechnology projects.

ENRICHING MEDIA MERCHANDISE SARANA PENUNJANG PROMOSI STUDI KASUS PADA BOOKSTORE

CCIT Journal ◽  
2014 ◽  
Vol 7 (3) ◽  
pp. 420-436
Author(s):  
Dewi Immaniar ◽  
Sudaryono Sudaryono ◽  
Ayu Ningrum
Keyword(s):  
Visual Media ◽  
Test Duration ◽  
Media Campaign ◽  
Business World ◽  
Retail Business ◽  
The Media ◽  
Media Types ◽  
The Times ◽  
A Company

Talk about retail business can not be separated from the importance of service to consumers and good quality goods . But at the present time due to intense competition in the business world , the service and quality of goods is not enough to be able to increase revenue and attract customers loyal . This makes companies think hard to survive and stable in the business . One of them is by using a media campaign in this regard more toward print or visual media is indirectly felt the value of their effectiveness in communicating product marketing programs . PT . Times Prima Indonesia is a company engaged in the retail book with the name of the Times bookstores . Based on the analysis of the company’s problems requires additional media types supporting more varied and creatif promotion of existing ones, which will be used as a complement and a media campaign as well as to enrich the data renewal campaign design to capture the interest of consumers in which one form of the media campaign is shaped merchandise . Therefore , do Enriching ( enrich ) media campaign merchandise before it is less varied and has not formed a company image . The methodology used is the analysis, observation and design . Besides the new design has been tested with the implemented test duration for 6 months, and greatly increases the perceived contribution , this is evidenced by the chart sales increasing each month.

Activated carbon as a better habitat for water and wastewater treatment microorganisms

2000 ◽  
Vol 42 (12) ◽  
pp. 149-154 ◽  
Author(s):  
M. Okada ◽  
H. Morinaga ◽  
W. Nishijima
Keyword(s):  
Batch Culture ◽  
Bacterial Growth ◽  
Membrane Filter ◽  
Synthetic Medium ◽  
Dialysis Tubing ◽  
Water And Wastewater Treatment ◽  
Batch Cultures ◽  
K 12 ◽  
Bacterial Yield ◽  
Adsorption Desorption

Effects of PAC on bacterial activity were evaluated by sequencing batch cultures (20 hours each) of E.coli K-12 on synthetic medium containing glucose as a sole carbon source. Four suspended sequencing batch culture systems were operated; CP: cultures supplemented with PAC, CR: cultures with removal of metabolites by PAC at the end of each batch culture, CD: cultures supplemented with PAC in dialysis tubing to separate from E.coli, and CC: cultures without PAC (control). The supernatant of each batch culture was filtered through a membrane filter (0.2 μm) and was mixed with the same volume of fresh medium to be used as the medium for the next batch culture. The sequencing batch cultures were repeated three times for all the systems. The bacterial growth in CC was inhibited with the increase in the number of batch cultures. Although a significant amount of metabolites was accumulated in the 3rd batch culture of CC, little accumulation was noted in the 3rd batch culture of CP. No growth inhibition was noted in CP for all the batch cultures. The little differences in the bacterial yield and metabolite accumulation between CR and CD suggested that adsorption/desorption of metabolites with PAC did not play a major role in bacterial growth. PAC addition may partly stimulate the growth by the removal of growth inhibiting metabolites. However, the fact that CP showed higher yield than CR and CD indicated that the contact between bacteria and PAC plays a significant role in the growth of bacteria.

scholarly journals “As quiet as a mouse”: Media use in Azerbaijan

2020 ◽  
Vol 45 (s1) ◽  
pp. 893-911
Author(s):  
Ilgar Seyidov
Keyword(s):  
Soviet Union ◽  
Media Use ◽  
Structured Interview ◽  
Soviet Period ◽  
Political Systems ◽  
Media System ◽  
The Soviet Union ◽  
Supportive Role ◽  
The Media ◽  
The One

AbstractDuring the Soviet period, the media served as one of the main propagandist tools of the authoritarian regime, using a standardized and monotype media system across the Soviet Republics. After the collapse of the Soviet Union, 15 countries became independent. The transition from Soviet communism to capitalism has led to the reconstruction of economic, socio-cultural, and political systems. One of the most affected institutions in post-Soviet countries was the media. Media have played a supportive role during rough times, when there was, on the one hand, the struggle for liberation and sovereignty, and, on the other hand, the need for nation building. It has been almost 30 years since the Soviet Republics achieved independence, yet the media have not been freed from political control and continue to serve as ideological apparatuses of authoritarian regimes in post-Soviet countries. Freedom of speech and independent media are still under threat. The current study focuses on media use in Azerbaijan, one of the under-researched post-Soviet countries. The interviews for this study were conducted with 40 participants living in Nakhichevan and Baku. In-depth, semi-structured interview techniques were used as research method. Findings are discussed under six main themes in the conclusion.

scholarly journals Osmoadaptation Strategy of the Most Halophilic Fungus, Wallemia ichthyophaga, Growing Optimally at Salinities above 15% NaCl

2013 ◽  
Vol 80 (1) ◽  
pp. 247-256 ◽  
Author(s):  
Janja Zajc ◽  
Tina Kogej ◽  
Erwin A. Galinski ◽  
José Ramos ◽  
Nina Gunde-Cimerman
Keyword(s):  
Biomass Production ◽  
Osmotic Shock ◽  
Model Organism ◽  
Salinity Range ◽  
Growth Media ◽  
Optimal Salinity ◽  
Content Type ◽  
Batch Cultures ◽  
Specific Growth Rates ◽  
Optimum Salinity

ABSTRACTWallemia ichthyophagais a fungus from the ancient basidiomycetous genusWallemia(Wallemiales, Wallemiomycetes) that grows only at salinities between 10% (wt/vol) NaCl and saturated NaCl solution. This obligate halophily is unique among fungi. The main goal of this study was to determine the optimal salinity range for growth of the halophilicW. ichthyophagaand to unravel its osmoadaptation strategy. Our results showed that growth on solid growth media was extremely slow and resulted in small colonies. On the other hand, in the liquid batch cultures, the specific growth rates ofW. ichthyophagawere higher, and the biomass production increased with increasing salinities. The optimum salinity range for growth ofW. ichthyophagawas between 15 and 20% (wt/vol) NaCl. At 10% NaCl, the biomass production and the growth rate were by far the lowest among all tested salinities. Furthermore, the cell wall content in the dry biomass was extremely high at salinities above 10%. Our results also showed that glycerol was the major osmotically regulated solute, since its accumulation increased with salinity and was diminished by hypo-osmotic shock. Besides glycerol, smaller amounts of arabitol and trace amounts of mannitol were also detected. In addition,W. ichthyophagamaintained relatively small intracellular amounts of potassium and sodium at constant salinities, but during hyperosmotic shock, the amounts of both cations increased significantly. Given our results and the recent availability of the genome sequence,W. ichthyophagashould become well established as a novel model organism for studies of halophily in eukaryotes.

Media Systems in the Digital Age: An Empirical Comparison of 30 Countries

2022 ◽  
Author(s):  
Edda Humprecht ◽  
Laia Castro Herrero ◽  
Sina Blassnig ◽  
Michael Brüggemann ◽  
Sven Engesser
Keyword(s):  
Cluster Analysis ◽  
Empirical Evidence ◽  
Information Technologies ◽  
Digital Age ◽  
Online News ◽  
Empirical Comparison ◽  
Media Freedom ◽  
Media System ◽  
The Media ◽  
Media Systems

Abstract Media systems have changed significantly as a result of the development of information technologies. However, typologies of media systems that incorporate aspects of digitalization are rare. This study fills this gap by identifying, operationalizing, and measuring indicators of media systems in the digital age. We build on previous work, extend it with new indicators that reflect changing conditions (such as online news use), and include media freedom indicators. We include 30 countries in our study and use cluster analysis to identify three clusters of media systems. Two of these clusters correspond to the media system models described by Hallin and Mancini, namely the democratic-corporatist and the polarized-pluralist model. However, the liberal model as described by Hallin and Mancini has vanished; instead, we find empirical evidence of a new cluster that we call “hybrid”: it is positioned in between the poles of the media-supportive democratic-corporatist and the polarized-pluralist clusters.

scholarly journals Newspaper attention and policy activities in Spain

2013 ◽  
Vol 33 (1) ◽  
pp. 65-88 ◽  
Author(s):  
Laura Chaqués Bonafont ◽  
Frank R. Baumgartner
Keyword(s):  
Policy Process ◽  
Front Page ◽  
Political Leaders ◽  
Daily Newspapers ◽  
Media Attention ◽  
Comprehensive Database ◽  
Media System ◽  
The Media ◽  
Over Time

AbstractSpain's newspapers are characterised by strong partisan identities. We demonstrate that the two leading newspapers nonetheless show powerful similarities in the topics of their coverage over time. The media system is strongly related to the policy process and it shows similar levels of skew (attention focuses on just a few topics) and friction (attention lurches rapidly from topic to topic) as others have shown for policy processes more generally. Further, media attention is significantly related to parliamentary activities. Oral questions in parliament track closely with media attention over time. Our assessment is based on a comprehensive database of all front-page stories (over 95,000 stories) in El País and El Mundo, Spain's largest daily newspapers, and all 7,446 oral questions from 1996 to 2009. The paper shows that explanations of friction and skew in governmental activities should incorporate media dynamics as well. Political leaders are clearly sensitive to media salience.

scholarly journals System medialny współczesnego Izraela

2018 ◽  
Vol 8 ◽  
pp. 119-132
Author(s):  
Kacper Kosma Kocur
Keyword(s):  
Current Situation ◽  
Political Orientation ◽  
The State ◽  
The Internet ◽  
Radio Stations ◽  
Media System ◽  
The Press ◽  
History Of ◽  
The Media ◽  
State Of Israel

The media system in Israel todayThe paper examines the media system in the state of Israel. It takes into account both the history of the media — from the press through radio and television to the internet — and the current situation. The author describes the most important Israeli media: newspapers, television and radio stations, as well as websites, taking into consideration their popularity on the market, political orientation and importance in Israel’s media world.

scholarly journals Medier og selvstændighed i Grønland

Politik ◽  
2019 ◽  
Vol 22 (1) ◽  
Author(s):  
Signe Ravn-Højgaard
Keyword(s):  
Nation Building ◽  
Digital Platforms ◽  
The Public ◽  
Media Regulation ◽  
Media System ◽  
The Media ◽  
Streaming Services ◽  
Potential Impact ◽  
Media Systems ◽  
Public Economy

This article discusses the potential impact of Greenlandic independence on Greenland’s media system and suggests initiatives necessary for maintaining strong Greenlandic media in the future. Using Manuel Puppis' (2009) theory of the characteristics of small media systems, the Greenlandic media system is described. It is shown that it is built with the following aim in mind: the media should support the Greenlandic society by being independent and diverse, strengthening the Greenlandic language, and providing quality journalism that can heighten the public debate. However, as a small media system it is vulnerable to global tendencies where legacy media lose users and advertisers to digital platforms like Facebook and streaming services. The article argues that the vulnerability of the Greenlandic media system could increase if independence leads to a tighter public economy, impeding the media's ability to support Greenlandic society and culture. An interventionist media regulation could, therefore, be a prerequisite for a strong Greenlandic media system that can act as a unifying and nation-building institution.

The State and Media Financing: Bulgaria and the Mediterranean Media Model

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Ivo Indjov ◽  
◽  
◽  
Keyword(s):  
Latin American ◽  
The State ◽  
State Funding ◽  
Communist Country ◽  
Media System ◽  
Role Of The State ◽  
Public Media ◽  
The Media ◽  
The Mediterranean ◽  
The Government

The study examines the applicability of the comparative framework of Hallin and Mancini (2004) with their three models of media‒politics relations (Mediterranean or Polarized Pluralist Model, North/ Central European or Democratic Corporatist Model, and North Atlantic or Liberal Model) to a post-communist country like Bulgaria. The answer to this question is sought through a study of the role of the state in relation to the media system, particularly the state funding of media in its various forms. The analysis leads to the conclusion that the Bulgarian media system is most similar to the Mediterranean Model due to the power of еtatism (the state finances public media, and the government buys media love through state and municipal advertising). At the same time, ineffective media regulation favors media concentration and the instrumentalization of large government media groups. The processes of rapid liberalization, privatization and deregulation in the media sector after 1989 brought Bulgaria closer to the countries included in the Liberal Model. Therefore, its media system is hybrid to some extent, but the similarities with the Mediterranean Model remain in the lead. The clientelism through which they are tamed, resp. corrupt the media, brings Bulgaria closer to the Latin American countries where it is much stronger than in the Mediterranean region (Hallin, Papathanassopoulos 2002). The concluding part predicts that, in the future, the analysis of the Bulgarian media system can be enhanced with a study of the applicability of the concepts of the “captured liberal model” of the media (in Latin America) and the “captured media” in the post-communist world.

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