Genome-wide identification and analysis of Oleosin gene family in four cotton species and its involvement in oil accumulation and germination

Abstract Background Cotton is not only a major textile fiber crop but also a vital oilseed, industrial, and forage crop. Oleosins are the structural proteins of oil bodies, influencing their size and the oil content in seeds. In addition, the degradation of oleosins is involved in the mobilization of lipid and oil bodies during seed germination. However, comprehensive identification and the systematic analysis of the Oleosin gene (OLEOs) family have not been conducted in cotton. Results An in-depth analysis has enabled us to identify 25 and 24 OLEOs in tetraploid cotton species G. hirsutum and G. barbadense, respectively, while 12 and 13 OLEOs were identified in diploid species G. arboreum and G. raimondii, respectively. The 74 OLEOs were further clustered into three lineages according to the phylogenetic tree. Synteny analysis revealed that most of the OLEOs were conserved and that WGD or segmental duplications might drive their expansion. The transmembrane helices in GhOLEO proteins were predicted, and three transmembrane models were summarized, in which two were newly proposed. A total of 24 candidate miRNAs targeting GhOLEOs were predicted. Three highly expressed oil-related OLEOs, GH_A07G0501 (SL), GH_D10G0941 (SH), and GH_D01G1686 (U), were cloned, and their subcellular localization and function were analyzed. Their overexpression in Arabidopsis increased seed oil content and decreased seed germination rates. Conclusion We identified OLEO gene family in four cotton species and performed comparative analyses of their relationships, conserved structure, synteny, and gene duplication. The subcellular localization and function of three highly expressed oil-related OLEOs were detected. These results lay the foundation for further functional characterization of OLEOs and improving seed oil content.

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Genome-wide identification and comparative analysis of diacylglycerol kinase (DGK) gene family and their expression profiling in Brassica napus under abiotic stress

BMC Plant Biology ◽

10.1186/s12870-020-02691-y ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Fang Tang ◽

Zhongchun Xiao ◽

Fujun Sun ◽

Shulin Shen ◽

Si Chen ◽

...

Keyword(s):

Gene Family ◽

Metal Ion ◽

Oil Content ◽

Seed Oil ◽

Catalytic Domain ◽

Expression Patterns ◽

Cadmium Stress ◽

Pcr Analysis ◽

Evolutionary Analysis ◽

Seed Oil Content

Abstract Background Diacylglycerol kinases (DGKs) are signaling enzymes that play pivotal roles in response to abiotic and biotic stresses by phosphorylating diacylglycerol (DAG) to form phosphatidic acid (PA). However, no comprehensive analysis of the DGK gene family had previously been reported in B. napus and its diploid progenitors (B. rapa and B. oleracea). Results In present study, we identified 21, 10, and 11 DGK genes from B. napus, B. rapa, and B. oleracea, respectively, which all contained conserved catalytic domain and were further divided into three clusters. Molecular evolutionary analysis showed that speciation and whole-genome triplication (WGT) was critical for the divergence of duplicated DGK genes. RNA-seq transcriptome data revealed that, with the exception of BnaDGK4 and BnaDGK6, BnaDGK genes have divergent expression patterns in most tissues. Furthermore, some DGK genes were upregulated or downregulated in response to hormone treatment and metal ion (arsenic and cadmium) stress. Quantitative real-time PCR analysis revealed that different BnaDGK genes contribute to seed oil content. Conclusions Together, our results indicate that DGK genes have diverse roles in plant growth and development, hormone response, and metal ion stress, and in determining seed oil content, and lay a foundation for further elucidating the roles of DGKs in Brassica species.

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A genome-wide analysis of the lysophosphatidate acyltransferase (LPAAT) gene family in cotton: organization, expression, sequence variation, and association with seed oil content and fiber quality

BMC Genomics ◽

10.1186/s12864-017-3594-9 ◽

2017 ◽

Vol 18 (1) ◽

Cited By ~ 15

Author(s):

Nuohan Wang ◽

Jianjiang Ma ◽

Wenfeng Pei ◽

Man Wu ◽

Haijing Li ◽

...

Keyword(s):

Gene Family ◽

Oil Content ◽

Sequence Variation ◽

Seed Oil ◽

Fiber Quality ◽

Seed Oil Content ◽

Genome Wide Analysis ◽

Genome Wide ◽

A Genome

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Correlation and path analysis of yield and yield components in winter rapeseed

Genetika ◽

10.2298/gensr2101157r ◽

2021 ◽

Vol 53 (1) ◽

pp. 157-166

Author(s):

Velimir Radic ◽

Igor Balalic ◽

Milos Krstic ◽

Ana Marjanovic-Jeromela

Keyword(s):

Seed Germination ◽

Path Analysis ◽

Protein Content ◽

Seed Yield ◽

Seed Weight ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content ◽

Correlation And Path Analysis ◽

Positive Direct

Five different rapeseed genotypes were tested. The following traits were considered: seed yield, seed germination, seed oil content, 1000 seed weight and seed protein content and they were used to determine whether there is significant or nonsignificant correlation. Highest average results were determined in genotype G-3 (yield 2.544 kg; germination 90%; 1000 seed weight 4,30 g; oil content 45,31% and protein content 19,83%) while G-5 (1.838 kg; 87%; 3,50 g; 44,77% and 17,28%) had the lowest average result of observed parameters. Highly significant positive correlations were found while comparing seed yield with 1000 seed weight (0.753**) and protein content (0.726**). High significant positive correlation were also determined comparing seed germination with 1000 seed weight (0.832**) and protein content (0.892**). Also high significant correlation was determined comparing 1000 seed weight and protein content (0.812**). Positive significant correlations were found by comparing seed yield and seed germination (0.644*). Path analysis indicated highest significant positive direct effect of 1000 seed weight (0.716**) and protein content (0.666**) on seed yield. High but negative significant value had seed germination (-0.645**). In the study of indirect effects on seed yield, none of significant effects were determined. The study of direct effects on oil content showed that the seed germination had high significant positive effect (-1.296**) on oil content. Significant but hot high effect has seed yield (0.556*). In other two traits it was determined negative high significant effects (1000 seed weight - 0.797** and protein content - 0.717**) on seed oil content. The existence of indirect significant effects on oil content in four cases was determined.

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Bioinformatics analysis of <italic>SnRK</italic> gene family and its relation with seed oil content of <italic>Brassica napus</italic> L.

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2021.04108 ◽

2020 ◽

Vol 47 (3) ◽

pp. 416-426

Author(s):

Jing-Quan TANG ◽

Nan WANG ◽

Jie GAO ◽

Ting-Ting LIU ◽

Jing WEN ◽

...

Keyword(s):

Brassica Napus ◽

Gene Family ◽

Oil Content ◽

Seed Oil ◽

Bioinformatics Analysis ◽

Seed Oil Content

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Registration of ‘NC-Miller’ Soybean with High Yield and High Seed-Oil Content

Journal of Plant Registrations ◽

10.3198/jpr2012.01.0007crc ◽

2012 ◽

Vol 6 (3) ◽

pp. 294-297 ◽

Cited By ~ 2

Author(s):

J. W. Burton ◽

L. M. Miranda ◽

T. E. Carter ◽

D. T. Bowman

Keyword(s):

Oil Content ◽

Seed Oil ◽

High Yield ◽

Seed Oil Content

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Faculty Opinions recommendation of The homeobox gene GLABRA2 affects seed oil content in Arabidopsis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1031749.367855 ◽

2006 ◽

Author(s):

Erich Grotewold

Keyword(s):

Oil Content ◽

Seed Oil ◽

Homeobox Gene ◽

Seed Oil Content

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Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in Brassica napus L.

International Journal of Molecular Sciences ◽

10.3390/ijms22031033 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1033

Author(s):

Abirami Rajavel ◽

Selina Klees ◽

Johanna-Sophie Schlüter ◽

Hendrik Bertram ◽

Kun Lu ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Brassica Napus ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content ◽

Complex Interplay ◽

Brassica Napus L ◽

Data Set ◽

Developmental Switches

Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.

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