scholarly journals The recent trend in mycobacterial strain diversity among extra pulmonary lymph node tuberculosis and their association with drug resistance and the host immunological response in South India

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Shanmugam Sivakumar ◽  
Yuvaraj Chandramohan ◽  
Gokul Raj Kathamuthu ◽  
Gomathi Sekar ◽  
Devika Kandhasamy ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Lymph Node ◽  
Latin American ◽  
Tamil Nadu ◽  
The Body ◽  
Tuberculous Lymphadenitis ◽  
Drug Resistant ◽  
Cytokine Profiles ◽  
Pulmonary Lymph Node ◽  
Extra Pulmonary

Abstract Background Tuberculosis (TB) though primarily affects the lungs it may also affect the other parts of the body and referred as extra pulmonary (EPTB). This study is focused on understanding the genetic diversity and molecular epidemiology of Mycobacterium tuberculosis (M.tb) among tuberculous lymphadenitis (TBL), a form of EPTB patients identified in Chennai, Tamil Nadu. Methods The genetic diversity was identified by performing spoligotyping on the M.tb clinical isolates that were recovered from lymph node samples. A total of 71 M.tb isolates were recovered from extra pulmonary lymph node samples and subjected to Drug susceptibility testing and spoligotyping was carried out. In addition, immunological characterization from blood of same individuals from whom M.tb was isolated was carried out between the two major lineages groups East African Indian 3 (EAI3) and non-EAI3 strains by ELISA. The results of spoligotyping patterns were compared with the world Spoligotyping Database of Institute Pasteur de Guadeloupe (SpolDB4). Results We found 41 spoligotype patterns and their associated lineages. Out of 41 spoligotype pattern, only 22 patterns are available in the spoldB4 database with Spoligotype international Type (SIT) number and remaining patterns were orphan strains without SIT number. The most predominant spoligotype lineage that was found in lymph node sample in this region of India was EAI (36), followed by central Asian strain (CAS) (6), T1 (5), Beijing (3), Latin American & Mediterranean (LAM) (2), U (1), X2 (1) and orphan (22). In addition to EAI, CAS and Beijing, our study identified the presence of orphan and unique spoligotyping patterns in Chennai region. We observed six drug resistant isolates. Out of six drug resistant isolates, four were resistant to isoniazid drug and associated with EAI family. Moreover, we observed increased levels of type 2 and type 17 cytokine profiles between EAI3 and non-EAI family, infected individuals. Conclusions The study confirms that EAI lineage to be the most predominant lineages in EPTB patients with lymphadenitis and were found to have increased type 1 and type 17 proinflammatory cytokine profiles.

scholarly journals Comparison of Acid Fast Bacilli Smear and Culture for the Diagnosis of Extra-Pulmonary Tuberculosis

2004 ◽  
Vol 43 (155) ◽  
Author(s):  
Basista Rijal ◽  
P Ghimire ◽  
N R Tuladhar
Keyword(s):  
Lymph Node ◽  
Pulmonary Tuberculosis ◽  
Pericardial Fluid ◽  
The Body ◽  
Diagnostic Methods ◽  
Isolation Rate ◽  
Extra Pulmonary Tuberculosis ◽  
Acid Fast Bacilli ◽  
New Methods ◽  
Extra Pulmonary

The study was conducted with the objectives to compare the Acid Fast Bacilli (AFB) staining and Culture for the diagnosis of extra-pulmonary tuberculosis and to find out the burden of extra-pulmonary tuberculosis from the different site of the body in suspected TB patients. The standard Ziehl-Neelsen Technique was done for AFB staining and culture was done in Ogawa Medium. Of the 292 extra-pulmonary samples examined by AFB smear only 2(0.7%) were positive and of the 1058 extra-pulmonary samples cultured 20 (1.9%) were positive. The isolation rate for extra-pulmonary samples was three fold higher in culture in comparison to AFB Smear. The confirmation rate of extra-pulmonary tuberculosis was approximately 1/ 8th of the pulmonary tuberculosis by conventional bacteriological diagnostic methods. Of the extra-pulmonary tuberculosis renal, endometrial or pelvic and cold abscess were common in this study. M.tuberculosis was also isolated from peritoneal fluid, pericardial fluid, Synovial fluid, lymph node and cerebro spinal fluids only by culture. In conclusion, the standard AFB culture has significant role for diagnosis of extra-pulmonary tuberculosis, even though the conventional bacteriological technique could diagnose very low number of extra-pulmonary tuberculosis. The new methods of diagnosis should be considered to confirm more extra- pulmonary  tuberculosis.Key Words: Extra-pulmonary tuberculosis, AFB Smear, Culture, Diagnosis, Nepal.

scholarly journals Pattern of extra pulmonary tuberculosis among urban population with special reference to CBNAAT as a diagnostic tool: a retrospective study at a tertiary care hospital of Odisha, India

2018 ◽  
Vol 6 (10) ◽  
pp. 3375
Author(s):  
C. Mohan Rao ◽  
Bhaskar Thakur ◽  
Saswat Subhankar ◽  
D. P. Dash
Keyword(s):  
Lymph Node ◽  
Tertiary Care Hospital ◽  
Diagnostic Yield ◽  
Point Of Care ◽  
Tertiary Care ◽  
Pulmonary Involvement ◽  
The Body ◽  
Nucleic Acid Amplification ◽  
Care Hospital ◽  
Extra Pulmonary

Background: Diagnosis of Extra-pulmonary TB (EPTB) is a challenge. Authors wanted to assess the sites of extra-pulmonary involvement during 2013-2017 in a tertiary care hospital cum medical college. Authors also wanted to evaluate the role of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) in diagnosis of EPTB and compare its efficacy with AFB Culture.Methods: Total 470 EPTB cases diagnosed between 2013 and 2017 from 840 TB treatment records maintained in designated microscopy centre. Specific samples from appropriate sites were taken up for smear for AFB, CBNAAT and AFB culture.Results: There was incremental detection and registration in both TB and EPTB cases from 96 and 50 cases in 2013 to 246 and 150 cases in 2017 respectively. Among the total 470 EPTB cases in 2013-2017 (55.9%), lymph node followed by pleura and abdomen were the organs having maximum involvement. Bone involvement was more witnessed in adult male than children (p <0.05). There was male preponderance. CBNAAT results were 100 % sensitive and 87.5% specific. Lymph node samples and pus elsewhere in the body had much better diagnostic yield than serous effusions.Conclusions: Awareness and availability of diagnostic services in tertiary care institutions has lead to increased reporting of EPTB under RNTCP services. CBNAAT can be also be utilized as a point of care testing for lymph node aspirate and pus specimen.

Scanning electron microscopy of freeze-fractured prescapular lymph node of caprine

1984 ◽  
Vol 42 ◽  
pp. 244-245
Author(s):  
O. Faroon ◽  
F. Al-Bagdadi ◽  
T. G. Snider ◽  
C. Titkemeyer
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Lymphatic System ◽  
Three Dimensional ◽  
Meat Products ◽  
The Body ◽  
Morphological Data ◽  
The World ◽  
Cellular Constituent ◽  
Scanning Electron

The lymphatic system is very important in the immunological activities of the body. Clinicians confirm the diagnosis of infectious diseases by palpating the involved cutaneous lymph node for changes in size, heat, and consistency. Clinical pathologists diagnose systemic diseases through biopsies of superficial lymph nodes. In many parts of the world the goat is considered as an important source of milk and meat products.The lymphatic system has been studied extensively. These studies lack precise information on the natural morphology of the lymph nodes and their vascular and cellular constituent. This is due to using improper technique for such studies. A few studies used the SEM, conducted by cutting the lymph node with a blade. The morphological data collected by this method are artificial and do not reflect the normal three dimensional surface of the examined area of the lymph node. SEM has been used to study the lymph vessels and lymph nodes of different animals. No information on the cutaneous lymph nodes of the goat has ever been collected using the scanning electron microscope.

Prognostic Implications of Lymph Node Metastases in Carcinoma of the Body and Tail of the Pancreas

Pancreas ◽  
2011 ◽  
Vol 40 (7) ◽  
pp. 1029-1033 ◽  
Author(s):  
Tevfik T. Sahin ◽  
Tsutomu Fujii ◽  
Mitsuro Kanda ◽  
Shunji Nagai ◽  
Yasuhiro Kodera ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastases ◽  
The Body ◽  
Node Metastases ◽  
Prognostic Implications

scholarly journals Imaging of Central Nervous System Tuberculosis

2017 ◽  
Vol 2 (2) ◽  
pp. 84-90
Author(s):  
Anggraini Dwi Sensusiati
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Mycobacterium Tuberculosis ◽  
Lymph Node ◽  
Ct Scan ◽  
Spinal Tuberculosis ◽  
Bacterial Disease ◽  
Pulmonary Tb ◽  
Intracranial Tuberculoma ◽  
Extra Pulmonary

Tuberculosis, or TB, is an infectious bacterial disease caused by Mycobacterium tuberculosis. TB can occur in pulmonary or extra pulmonary. Extra pulmonary TB occurs in locations other than the lung, such as larynx, lymph node, pleura, brain, kidneys and bones. CT Scan can detect intracranial tuberculoma, and MRI is the best method to detect the abnormality in spinal tuberculosis.

scholarly journals Imaging Sensitive and Drug-Resistant Bacterial Infection with [11C]-TMP: In Vitro and First-in-Human Evaluation

2021 ◽  
Author(s):  
Iris K Lee ◽  
Daniel A Jacome ◽  
Joshua K Cho ◽  
Vincent Tu ◽  
Anthony Young ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Mammalian Cells ◽  
Bacterial Infections ◽  
The Body ◽  
Response Monitoring ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Critical Question ◽  
In Vitro Uptake

Recently, several molecular imaging strategies have developed to image bacterial infections in humans. Nuclear approaches, specifically positron emission tomography (PET), affords sensitive detection and the ability to non-invasively locate infections deep within the body. Two key radiotracer classes have arisen: metabolic approaches targeting bacterial specific biochemical transformations, and antibiotic-based approaches that have inherent selectivity for bacteria over mammalian cells. A critical question for clinical application of antibiotic radiotracers is whether resistance to the template antibiotic abrogates specific uptake, thus diminishing the predictive value of the diagnostic test. We recently developed small-molecule PET radiotracers based on the antibiotic trimethoprim (TMP), including [11C]-TMP, and have shown their selectivity for imaging bacteria in preclinical models. Here, we measure the in vitro uptake of [11C]-TMP in pathogenic susceptible and drug-resistant bacterial strains. Both resistant and susceptible bacteria showed similar in vitro uptake, which led us to perform whole genome sequencing of these isolates to identify the mechanisms of TMP resistance that permit retained radiotracer binding. By interrogating these isolate genomes and a broad panel of previously sequenced strains, we reveal mechanisms where uptake or binding of TMP radiotracers can potentially be maintained despite the annotation of genes conferring antimicrobial resistance. Finally, we present several examples of patients with both TMP-sensitive and drug-resistant infections in our first-in-human experience with [11C]-TMP. This work underscores the ability of an antibiotic radiotracer to image bacterial infection in patients, which may allow insights into human bacterial pathogenesis, infection diagnosis, and antimicrobial response monitoring.

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