scholarly journals Prevalence of group B streptococcus colonization in pregnant women in Jiangsu, East China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yanmei Ge ◽  
Fei Pan ◽  
Rui Bai ◽  
Yuan Mao ◽  
Wenli Ji ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Bacterial Culture ◽  
Clinical Laboratory ◽  
Group B Streptococcus ◽  
East China ◽  
Quick Method ◽  
Positive Rate ◽  
The Difference ◽  
Group B ◽  
Conventional Detection

Abstract Background Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China, and revealed the difference of GBS infection between culture and PCR. Methods A total of 16,184 pregnant women at 34 to 37 weeks’ gestation aged 16–47 years were recruited from Nanjing Kingmed Center for Clinical Laboratory. Nine thousand twenty-two pregnant women received GBS screening by PCR detection only. Seven thousand one hundred sixty-two pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance. Results The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. Colonization rate was highest in the “25–29 years” age group. The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin, and levofloxacin was 77.5, 68.3, and 52.2%, respectively. Conclusions This study revealed the data on the prevalence of GBS colonization in pregnant women at 34 to 37 weeks’ gestation in Jiangsu, East China. It compared the difference of the sensitivity to detect GBS between PCR and culture. PCR was expected to become a quick method in pregnancy women conventional detection of GBS infection.

scholarly journals Prevalence of Group B Streptococcus Colonization in Pregnant Women in Jiangsu, East China

2020 ◽  
Author(s):  
Rui Wang ◽  
Hongmei Qiu ◽  
Ge Yanmei ◽  
Fei Pan ◽  
Shuhui Bian
Keyword(s):  
Pregnant Women ◽  
Group B Streptococcus ◽  
East China ◽  
Age Difference ◽  
Quick Method ◽  
Positive Rate ◽  
The Difference ◽  
Group B ◽  
Over 40 Years ◽  
Conventional Detection

Abstract Background: Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China and reveled the difference of GBS infection between culture and PCR . Methods: A total of 16,184 pregnant women at 34 to 37 weeks, gestation aged 16–47 years were recruited from Nanjing Kingmed Diagnostics. There were 9022 pregnant women receiving GBS screening by PCR detection only. 7162 pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance. Results: The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. There was no significant age difference of GBS infection, but the 25-29 age group and people aged over 40 years should pay more attention . The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin and levofloxacin was 77.5%, 68.3% and 52.2%, respectively. Conclusions: This study revealed the data on the prevalence of GBS colonization in pregnant women in Jiangsu, East China. And it compared the difference of GBS infection between culture and PCR. PCR was expectd to become a quick method in pregnancy women conventional detection of GBS infection.

scholarly journals Prevalence of Group B Streptococcus Colonization in Pregnant Women in Jiangsu, East China

2019 ◽  
Author(s):  
Rui Wang ◽  
Hongmei Qiu ◽  
Ge Yanmei ◽  
Fei Pan ◽  
Shuhui Bian
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Neonatal Sepsis ◽  
Susceptibility Testing ◽  
Bacterial Culture ◽  
Group B Streptococcus ◽  
East China ◽  
Age Group ◽  
The Difference ◽  
Group B

Abstract Background : Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China. Methods: A total of 16,184 pregnant women at 34 to 37 weeks , gestation aged 16–47 years were recruited from Nanjing Kingmed Diagnostics, including 9022 pregnant women who received GBS screening by PCR detection and 7162 by bacterial culture, antimicrobial susceptibility testing was performed on GBS positive samples. Results: The overall GBS prevalence was 8.7% for pregnant women studied by PCR and 3.5% by culture. The 25-29 age group had the highest rate of GBS colonization in the pregnant women. The prevalence of resistance to erythromycin, clindamycin and levofloxacin was 77.5%, 68.3% and 52.2%, respectively. Conclusions: This study revealed the prevalence characteristics of GBS in pregnant women and the difference of GBS colonization between culture and PCR in Jiangsu province.

scholarly journals Prevalence and molecular characterization of group B streptococcus in pregnant women from hospitals in Ohangwena and Oshikoto regions of Namibia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Erastus Lafimana Haimbodi ◽  
Munyaradzi Mukesi ◽  
Sylvester Rodgers Moyo
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Group B Streptococcus ◽  
Tetracycline Resistance ◽  
Geographic Location ◽  
Molecular Characteristics ◽  
Capsular Type ◽  
Type Ia ◽  
Positive Rate ◽  
Group B

Abstract Background The main purpose of this study was to investigate the prevalence rate, antimicrobial susceptibility patterns and molecular characteristics of Streptococcus agalactiae isolated from pregnant women at 35 weeks of gestation and above, who attended antenatal screening at selected hospitals in Ohangwena and Oshikoto regions of Namibia. Results Out of 210 women screened for Group B Streptococcus (GBS), 12 (5.7%) were colonised of which 25.0% were colonised rectovaginally, 58.0% vaginally and 17.0% rectally. No significant association was reported between GBS colonisation and maternal age, geographic location, marital status, education, employment, parity, still births and miscarriages (P values > 0.05). Antimicrobial susceptibility was reported at 100% for ampicillin, penicillin & ceftriaxone which are commonly used for empiric treatment of infection with GBS. Resistance to tetracycline was reported at 100%. Tetracycline resistance gene tet(M) was present in 88.9% of the isolates only and none of the isolates presented with tet(O). Polysaccharide capsular type Ia was found in 9(50%) and Ib was found in 1(5.5%) of the total isolates. The remaining isolates were not typeable using PCR. Conclusion Streptococcus agalactiae’s positive rate was 5.7% among the pregnant women examined. Socio-demographic and obstetric factors had no influence on GBS colonisation (P values > 0.05). No resistance was reported to ampicillin, penicillin and ceftriaxone. No sensitivity was reported to tetracycline. Fifty percent of the isolates were capsular type Ia, 5.5% were type Ib and 44.4% were not typeable using PCR. The study provides crucial information for informing policy in screening of GBS in pregnant women.

scholarly journals Reliable Detection of Group B Streptococcus in the Clinical Laboratory

2017 ◽  
Vol 55 (9) ◽  
pp. 2590-2598 ◽  
Author(s):  
Manuel Rosa-Fraile ◽  
Barbara Spellerberg
Keyword(s):  
Pregnant Women ◽  
Clinical Laboratory ◽  
Culture Media ◽  
Group B Streptococcus ◽  
Molecular Techniques ◽  
Diagnostic Tools ◽  
Species Determination ◽  
Flight Mass Spectrometry ◽  
Detection And Identification ◽  
Group B

ABSTRACTGroup B streptococcus (GBS) is a leading cause of invasive neonatal infections and a significant pathogen in immunocompromised adults. Screening to detect GBS colonization in pregnant women determines the need for antibiotic prophylaxis in that pregnancy. Efficient determination of the GBS colonization status of pregnant women is crucial. Methods that maximize the probability of GBS recovery are needed. The availability of technologies such as matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), molecular techniques, and chromogenic culture media, including Granada-type media, have changed the scenario for GBS detection and identification. This review presents and evaluates novel diagnostic tools, as well as classic identification techniques, for GBS species determination.

scholarly journals Prevalence and Molecular Characterization of Group B Streptococcus in Pregnant Women from Hospitals in Ohangwena and Oshikoto Regions of Namibia.

2020 ◽  
Author(s):  
Sylvester Rodgers Moyo ◽  
Erastus Lafimana Haimbodi ◽  
Munyaradzi Mukesi
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Group B Streptococcus ◽  
Tetracycline Resistance ◽  
Molecular Characteristics ◽  
Capsular Type ◽  
P Values ◽  
Type Ia ◽  
Positive Rate ◽  
Group B

Abstract BackgroundThe main purpose of this study was to investigate the prevalence rate, antimicrobial susceptibility patterns and molecular characteristics of Streptococcus agalactiae isolated from pregnant women at 35 weeks of gestation and above, who attended antenatal screening at selected hospitals in Ohangwena and Oshikoto regions of Namibia.ResultsTwelve (5.7%) of women screened were colonised by GBS, of which 25.0% were colonised rectovaginally, 58.0% vaginally and 17.0% rectally. No significant association was reported between GBS colonisation and maternal age, habitat, marital status, education, employment, parity, still births and miscarriages (P values >0.05). Antimicrobial susceptibility was reported at 100% for ampicillin, penicillin & ceftriaxone. Resistance to tetracycline was reported at 100%. Tetracycline resistance gene tet(M) was present in 88.9% of the isolates only and none of the isolates presented with tet(O). Polysaccharide capsular type Ia was found in 9(50%) and Ib was found in 1(5.5%) of the total isolates. The remaining isolates were not typeable using PCR.ConclusionStreptococcus agalactiae’s positive rate was 5.7% among the pregnant women examined. Socio-demographic and obstetric factors had no influence on GBS colonisation (P values >0.05). No resistance was reported to ampicillin, penicillin and ceftriaxone. No sensitivity was reported to tetracycline. Fifty (50%) of the isolates were capsular type Ia, 5.5% were type Ib and 44.4% were not typeable using PCR.

The Application of Realtime Fluorescence Quantitative PCR for Prenatal Screening of Group B Streptococcal Infections

2019 ◽  
Vol 2 (3) ◽  
pp. 12
Author(s):  
Changzhi Xu ◽  
Donglin Zhu ◽  
Zhizhi Xie ◽  
Yun Xi
Keyword(s):  
Quantitative Pcr ◽  
Prenatal Screening ◽  
Bacterial Culture ◽  
Group B Streptococcus ◽  
Gene Sequencing ◽  
Culture Method ◽  
Gold Standard Method ◽  
Fluorescence Quantitative Pcr ◽  
Positive Rate ◽  
Group B

Objective: In the prenatal screening, several different methods were used to detect the presence of group B streptococcus (GBS) infection, in this assay, the diagnostic value and clinical signifcance of the application of realtime fluorescent PCR were explored. Methods: A total of 86 women with 35-37 weeks pregnancy were enrolled, vaginal secretion samples were collected. Fluorescence PCR, bacterial culture and gene sequencing were used to detect whether there was GBS infection, and the results obtained were compared and analyzed. Results: 10 subjects were detected to be positive for GBS by fluorescence PCR (the positive rate was 11.6%), however, only 4 cases were positive for GBS by bacterial culture method (the positive rate was 4.7%). There was a statistically signifcant difference in the positive rate between the two methods (P<0.01). Compared with the results of gene sequencing, the detection of GBS infection by fluorescence PCR has an accuracy of 95.2%, and the sensitivity was 90.9% with 100% specificity. Conclusion: The application of realtime fluorescence quantitative PCR for the detection of GBS infection is signifcantly better than the use of bacterial culture method. Compared with the gold standard method (gene sequencing method), its detection effciency, accuracy, sensitivity and specifcity are relatively high. In summary, PCR for prenatal screening of GBS is worthy of promotion in clinical practice.

scholarly journals On the Safety of COOK Double Balloons to Promote Cervical Ripening in Pregnant Women With Group B Streptococcus Colonization

2021 ◽  
Author(s):  
Ping Ni ◽  
Weitao Yang ◽  
Lanting Yu ◽  
Hua Li ◽  
Lihui Huang ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Pregnant Women ◽  
Group B Streptococcus ◽  
Cervical Ripening ◽  
Control Group ◽  
Observation Group ◽  
Third Trimester ◽  
The Third ◽  
The Difference ◽  
Group B

Abstract Background: For pregnant women who develop complications during the third trimester of pregnancy, or who have not given birth naturally after more than 41 weeks of pregnancy, artificial induction of labor is needed in order to obtain a healthy outcome for both the mother and the child. The 2014 edition of the Guidelines for Promoting Cervical Maturation and Delivery in Late Pregnancy point out that the use of COOK cervical ripening balloons to mechanically dilate the cervix can be used in the third trimester to promote cervical ripening and labor induction [1]. The disadvantage is the risk of infection, premature rupture of membranes, and umbilical cord prolapse [2]. The safety of balloon induction for pregnant women colonized by group B streptococcus (GBS) is currently lacking in multi-center clinical research data. This article will study the safety of COOK double balloon induction in pregnant women colonized by GBS.Methods: A total of 1,681 pregnant women who used COOK double balloons for cervical ripening in Changsha Maternity and Child Health Hospital from September 2018 to September 2020 were selected as the research subjects, from which 125 cases with colonization of group B streptococcus in the reproductive tract were selected as the observation group. Pregnant women without group B streptococcus colonization (N = 1556) served as the control group. This study compares the two groups’ delivery methods, postpartum complications, and neonatal conditions. Results: The rate of transition to cesarean section in the observation group was slightly higher, and the difference was statistically significant (p = 0.049). The rate of postpartum hemorrhage was higher than that of the control group (p < 0.05). Although chorioamnionitis increased compared to the control group, the difference was not significant (p > 0.05). The comparison of newborn birth indicators between the two groups showed no statistically significant difference (p > 0.05). Conclusion: When pregnant women with colonization of group B streptococcus of the genital tract use the COOK double balloon to promote cervical ripening, the success rate of labor induction is high. Use of the balloon does not increase the cesarean section rate and the incidence of chorioamnionitis, nor does it increase the risk of neonatal infection. However, the risk of postpartum hemorrhage increases, and it is necessary to take active measures to reduce this risk.

scholarly journals Biomarkers for a histological chorioamnionitis diagnosis in pregnant women with or without group B streptococcus infection: a case-control study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jie Ren ◽  
Zhe Qiang ◽  
Yuan-yuan Li ◽  
Jun-na Zhang
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Peripheral Blood ◽  
Group B Streptococcus ◽  
Diagnostic Value ◽  
Adverse Outcomes ◽  
Intercellular Adhesion Molecule ◽  
Tnf Α ◽  
Group B ◽  
The Impact

Abstract Background Chorioamnionitis may cause serious perinatal and neonatal adverse outcomes, and group B streptococcus (GBS) is one of the most common bacteria isolated from human chorioamnionitis. The present study analyzed the impact of GBS infection and histological chorioamnionitis (HCA) on pregnancy outcomes and the diagnostic value of various biomarkers. Methods Pregnant women were grouped according to GBS infection and HCA detection. Perinatal and neonatal adverse outcomes were recorded with a follow-up period of 6 weeks. The white blood cell count (WBC), neutrophil ratio, and C-reactive protein (CRP) level from peripheral blood and soluble intercellular adhesion molecule-1 (sICAM-1), interleukin 8 (IL-8), and tumor necrosis factor α (TNF-α) levels from cord blood were assessed. Results A total of 371 pregnant women were included. Pregnant women with GBS infection or HCA had a higher risk of pathological jaundice and premature rupture of membranes and higher levels of sICAM-1, IL-8, and TNF-α in umbilical cord blood. Univariate and multivariate regression analysis revealed that sICMA-1, IL-8, TNF-α, WBC, and CRP were significantly related to an increased HCA risk. For all included pregnant women, TNF-α had the largest receiver operating characteristic (ROC) area (area: 0.841; 95% CI: 0.778–0.904) of the biomarkers analyzed. TNF-α still had the largest area under the ROC curve (area: 0.898; 95% CI: 0.814–0.982) for non-GBS-infected pregnant women, who also exhibited a higher neutrophil ratio (area: 0.815; 95% CI: 0.645–0.985) and WBC (area: 0.849; 95% CI: 0.72–0.978), but all biomarkers had lower value in the diagnosis of HCA in GBS-infected pregnant women. Conclusion GBS infection and HCA correlated with several perinatal and neonatal adverse outcomes. TNF-α in cord blood and WBCs in peripheral blood had diagnostic value for HCA in non-GBS-infected pregnant women but not GBS-infected pregnant women.

scholarly journals Poor Adherence to the Screening-Based Strategy of Group B Streptococcus Despite Colonization of Pregnant Women in Greece

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 418
Author(s):  
Maria Maroudia Berikopoulou ◽  
Aikaterini Pana ◽  
Theodota Liakopoulou-Tsitsipi ◽  
Nikos F. Vlahos ◽  
Vasiliki Papaevangelou ◽  
...  
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Pregnant Women ◽  
Late Onset ◽  
Group B Streptococcus ◽  
Culture Collection ◽  
Carrier Status ◽  
Cross Sectional ◽  
Screening Guidelines ◽  
Group B

Group B streptococcus (GBS) is a leading cause of serious neonatal infections. Maternal GBS colonization is associated with early- and late-onset neonatal disease (EOD/LOD). In Greece, a screening-based strategy is recommended, in which concurrent vaginal-rectal cultures should be obtained between 36 0/7 and 37 6/7 weeks’ gestation. We sought to examine the level of adherence to the GBS screening guidelines and estimate the prevalence of GBS colonization among pregnant women. Although in Greece the screening-based strategy is followed, we also examined known EOD risk factors and linked them to GBS colonization. A cross-sectional study of 604 women postpartum in three hospitals and maternity clinics was conducted. Following written informed consent, data were collected via a short self-completed questionnaire and review of patients’ records. In 34.6% of the enrolled pregnant women, no culture had been taken. Of the remaining, 12.8% had proper vaginal-rectal sample collections. The overall maternal colonization rate was 9.6%. At least one risk factor for EOD was identified in 12.6% of participants. The presence of risk factors was associated with positive cultures (p = 0.014). The rate of culture collection did not differ between women with or without an EOD risk factor. Adherence to a universal screening of pregnant women with vaginal-rectal cultures was poor. Despite probable underestimation of GBS carrier status, almost 1 in 10 participants were GBS positive during pregnancy. Screening of women with risk factors for EOD should, at least, be prioritized to achieve prevention and prompt intervention of EOD.

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