scholarly journals Tooth and temporary filling material fractures caused by Cavit, Cavit W and Coltosol F: an in vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bedram Djouiai ◽  
Thomas Gerhard Wolf
Keyword(s):  
In Vitro Study ◽  
Clinical Situation ◽  
Vitro Study ◽  
Rank Test ◽  
Control Group ◽  
Filling Material ◽  
Tooth Structure ◽  
Temporary Filling ◽  
Observation Period

Abstract Background Tooth fractures can occur after temporary inter-appointment endodontic filling, resulting in not preserving and thus extraction of the affected tooth. The purpose of this investigation was therefore to evaluate the tooth substance fracture potential given by the expansion of endodontic temporary filling materials. Methods Tooth and access cavities were prepared in 80 mandibular molars. Four groups of 20 teeth each (Cavit, Cavit W and Coltosol F and control) were included. To simulate a clinical situation, the teeth were endodontically pre-treated and a calcium hydroxide dressing was placed. The cavities were filled with the corresponding temporary filling material, with exception of the control group, and kept submerged in distilled water for 15 days. The teeth were examined every 24 h by two calibrated observers under a stereomicroscope (7.5×), fractures of the temporary filling material and tooth structure were photo-documented, and the results statistically analyzed. Kaplan–Meier survival analysis were calculated to illustrate (survival = no fracture) probabilities to evaluate the time when the temporary filling material, tooth structure or both together occurred. Log-rank test was performed in order to assess significant differences between the materials and the subgroups used. Results Fractures were observed only in the Coltosol F group (p < 0.01), at the end of the observation period, a total of 13 teeth (65%) showed temporary filling material and eight teeth (40%) showed tooth structure fractures. No fractures in the pulp chamber area were observed at the end of the observation period in any group. Conclusions Within the limitations of the current in vitro study, the results obtained suggest that tooth structure fractures caused by a temporary filling material can occur during endodontic treatment, thus compromising the success of the treatment.

scholarly journals Effect of topical fluoride on microshear bond strength of primary enamel to composite, microhardness of enamel and its surface morphology: An in vitro study

2019 ◽  
Vol 13 (4) ◽  
pp. 305-310
Author(s):  
Mina Biria ◽  
Sajedeh Namaei Ghasemi ◽  
Seyedeh Mahsa Sheikh-Al-Eslamian ◽  
Narges Panahandeh
Keyword(s):  
Bond Strength ◽  
Artificial Saliva ◽  
Morphological Characteristics ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Modes Of Failure ◽  
Group 3 ◽  
Microshear Bond Strength

Background. This in vitro study aimed to evaluate the microshear bond strength (μSBS), microhardness and morphological characteristics of primary enamel after treating with sodium fluoride (NaF) and acidulated phosphate fluoride (APF). Methods. Forty-eight primary canines were cut into mesial and distal sections and assigned to five groups randomly: group 1 (immersed in saliva as a control), group 2 (treated with NAF and immersed in saliva for 30 minutes), group 3 (treated with APF and immersed in saliva for 30 minutes), group 4 (treated with NAF and immersed in saliva for 10 days), and group 5 (treated with APF and immersed in saliva for 10 days). Composite resin (Filtek Z250) was bonded on the specimens (n=15) for measuring the μSBS. After storage in 37°C artificial saliva for 24 hours, µSBS and Vickers hardness tests (10 readings) were performed. The data were analyzed using one-way ANOVA and Kolmogorov-Smirnov, Levene’s and Tukey HSD tests (P<0.05). Morphological analysis of enamel and modes of failure were carried out under a scanning electron microscope (SEM) on two remaining specimens. Results. Significant differences in μSBS were only noted between groups 2 and 4 (P=0.024). Group 3 showed a significant decrease in hardness after storage in artificial saliva (P<0.001), with a significantly lower hardness than the other groups (P<0.001). The SEM observations showed irregular particles in groups 3 and 5; uniform, smooth and thin coats were seen in groups 2 and 4. Conclusion. Fluoride therapy with NaF and APF gels prior to restorative treatments had no adverse effects on the microshear bond strength.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

scholarly journals Comparative evaluation of force decay pattern in orthodontic active tiebacks exposed to five different mouth rinses: An in vitro Study

2020 ◽  
Vol 14 (4) ◽  
pp. 244-249
Author(s):  
Amir Hossein Mirhashemi ◽  
Atefe Saffar Shahroudi ◽  
Keyvan Shahpoorzadeh ◽  
Niloofar Habibi Khameneh
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Mouth Rinses ◽  
Force Decay ◽  
Force Relaxation ◽  
Decay Pattern ◽  
Initial Force ◽  
And Control

Background. This study compared the force decay pattern of two different orthodontic active tiebacks (ATBs) exposed to five different commercially available mouth rinses. Methods. In this in vitro study, 90 transparent ATBs and 90 gray ATBs were divided into six groups; one was the control group, and the others were exposed to one of these mouth rinses twice a day for 60 seconds: Listerine, chlorhexidine, Orthokin, Persica, and fluoride. The initial force of each ATB was 250 g at a 24-mm extension. The force of ATBs was measured on days 1, 7, 14, and 28 using a digital gauge. Results. The highest percentage of force loss was observed between days 14 and 28 (P<0.05). At the end of the study, the Persica group exhibited the highest force degradation in both ATB types. In the transparent ATBs, it was followed by Orthokin, Listerine, fluoride, chlorhexidine, and control groups, respectively. In the gray ATBs, Orthokin, chlorhexidine, control, Listerine, and fluoride groups exhibited the highest force decay in descending order. In some groups, the differences between transparent and gray ATBs were significant. In the control group, the force of transparent ATB was significantly higher than gray ones on days 7 and 14 but not significantly after four weeks. Conclusion. ATBs’ force degradation could be exacerbated by the use of some mouth rinses. There were some differences between force relaxation patterns of transparent and gray ATBs. The data could be beneficial in choosing appropriate O-rings for making ATBs.

scholarly journals An Evaluation of GuttaFlow2 in Filling Artificial Internal Resorption Cavities: An in vitro Study

2016 ◽  
Vol 17 (6) ◽  
pp. 445-450 ◽  
Author(s):  
Mohammad Y Hajeer ◽  
Yara Mohammad ◽  
Hisham Alafif ◽  
Oula Yassin
Keyword(s):  
Cross Sections ◽  
Root Canal ◽  
In Vitro Study ◽  
Free Flow ◽  
Control Group ◽  
Injection Technique ◽  
Filling Material ◽  
The Third ◽  
Gutta Percha

ABSTRACT Introduction Obturation of root canal with internal resorption represents a major challenge in Endodontics. In spite of that, usual obturation techniques are often employed without considering the best technique to solve this problem. The goal of this study was to investigate the ability of GuttaFlow2 in filling artificial internal resorption cavities. Materials and methods The study sample included 36 human upper central incisors that were prepared using Protaper system (F4). Internal resorption cavities were prepared by cutting each tooth at 7 mm from the apex and preparing hemispherical cavities on both the sides and then re-attaching them. The sample was randomly separated into three groups (n = 12 in each group). In the first group, thermal injection technique (Obtura II) was employed and served as the control group. In the second group, injection of cold free-flow obturation technique with a master cone (GF2-C) was employed, whereas in the third group injection of cold free-flow obturation without a master cone (GF2) was followed. The teeth were re-cut at the same level as before and examined under a stereomicroscope. Subsequently, the captured images were transferred to AutoCAD program to measure the percentage of total filling “TF,” gutta-percha “G,” sealer “S,” and voids “V” out of the total surface of the cross sections. Results All materials showed high filling properties in terms of “total filling,” ranging from 99.17% (for Obtura II) to 99.72% (for GF2-C). Regarding gutta-percha percentages of filling, they ranged from 83.15 to 83.93%, whereas those for the sealer ranged from 5.71 to 15.24%. GuttaFlow2 group with a master cone appeared to give the best results despite the insignificant differences among the three groups. Conclusion The GuttaFlow2 with a master cone technique seemed to be a promising filling material and gave results similar to those observed with Obtura II. It is recommended for use to obturate internal resorption cavities in clinical practice due to its good adaptability to root canal walls, ease of handling, and application. Clinical significance Internal resorption defects can be successfully filled with GuttaFlow2 material when supplemented with a master cone, and the results are comparable with those obtained with the Obtura II technique. How to cite this article Mohammad Y, Alafif H, Hajeer MY, Yassin O. An Evaluation of GuttaFlow2 in Filling Artificial Internal Resorption Cavities: An in vitro Study. J Contemp Dent Pract 2016;17(6):445-450.

scholarly journals Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Saeedeh Galledar ◽  
Robab Farhang ◽  
Malek Abazari ◽  
Parisa Negahdar
Keyword(s):  
Root Canal ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the  and MTA Fillapex test groups. While, the observed difference between  and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that  and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

scholarly journals Comparison of force decay pattern in orthodontic elastomeric chains and NiTi closed coil springs, affected by five different mouthwashes: An in vitro Study

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Amir Hossein Mirhashemi ◽  
Niloofar Habibi Khameneh ◽  
Keyvan Shahpoorzadeh ◽  
Atefe Saffar Shahroudi
Keyword(s):  
Reduction Rate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Force Decay ◽  
Decay Pattern ◽  
Force Reduction ◽  
Initial Force ◽  
Elastomeric Chains

Background: The aim of this study was to compare the force decay pattern of elastomeric chains and NiTi coil springs which were exposed to five different commercially available mouthwashes.Methods: In this in vitro study, 60 pieces of elastomeric chain (EC) and 60 NiTi closed coil springs (CS) were divided into 6 groups. The specimens were exposed to one of these mouthwashes twice a day for 60 seconds:  Listerine, chlorhexidine, Orthokin, Persica, fluoride and artificial saliva as the control group. The elastomeric chains and NiTi springs were stretched so that they exert the initial force of 250 gr. Their force was measured on the 1, 7, 14 and 28 following days by means of a digital gauge.Results: Elastomeric chains and coil springs had force decrease over time, but EC' force reduction was greater with the highest reduction rate in the first week. However, in the CS group, the force decrease wasn't statistically significant in the first two weeks.  After 28 days in the control groups, 49.8% of the initial force was remained in ECs while the value was 93.3% for CSs. In comparison between mouthwashes, in EC groups, in all mouthwashes except Persica, the remained force was statistically less than control group.Conclusion: Force degradation of elastomeric chains could be exacerbated by use of mouthwashes. About coil springs, force decay was also observed. However, it was not statistically significant. Force reduction was detectable after four weeks of coil springs usage, but in EC groups, the greatest reduction was after the first week.  

scholarly journals Dissolution capacity of orange and eucalyptus oils versus endodontic types of cement (in vitro study)

2022 ◽  
Vol 10 (19) ◽  
pp. 29-33
Author(s):  
Sebastián M. Ulloa-Alvarez ◽  
María E. Guerrero-Coello ◽  
José E. Torracchi-Carrasco
Keyword(s):  
In Vitro Study ◽  
Control Group ◽  
Filling Material ◽  
Cement Sample ◽  
Orange Oil ◽  
Eucalyptus Oil ◽  
Before And After ◽  
The Difference ◽  
Post Hoc

Introduction: Endodontic retreatment seeks reentry to the root canal system to remove all filling material, identification and correction of pathological or iatrogenic defects. Objective: This study aimed to compare the dissolving capacity of eucalyptus oil, orange oil, and distilled water versus three types of endodontic cements. Materials and methods: Two hundred and four stainless steel molds were fabricated, where each cement sample was placed to the edge and left to set in the incubator, simulating normal oral conditions for 7 days. Subsequently, the solubility of each cement was analysed by immersing the samples in eucalyptus and orange oil for ten minutes in a static and ultrasonic environment. The solubility of the cement was evidenced by the difference between the weight before and after contact with the oils, data were statistically analysed with the ANOVA test and the post hoc Tukey bootstrap means test with 1,000 repetitions. Results: The control group and eucalyptus oil having no significant effect (p>0.05), the ultrasonic environment was found to be effective (p<0.05), whereas orange oil had better dissolution effects (p=0.004). Conclusions: The most susceptible cement was Sealapex, while the least soluble was MTA-Fillapex in all the solvents studied.

scholarly journals Effects of a newly designed apparatus on orthodontic skeletal anchorage

2013 ◽  
Vol 07 (S 01) ◽  
pp. S083-S088 ◽  
Author(s):  
Murat Tozlu ◽  
Didem Nalbantgil ◽  
Fulya Ozdemir
Keyword(s):  
Cortical Bone ◽  
Lateral Displacement ◽  
Tangential Force ◽  
Testing Machine ◽  
In Vitro Study ◽  
Vitro Study ◽  
Favorable Effect ◽  
Control Group ◽  
Insertion Torque

ABSTRACT Objective: An appliance was designed to increase the cortical bone surface contact area of miniscrew implants (MSIs). The purpose of this in vitro study was to evaluate the effects of this appliance on the anchorage force resistance and the stability of orthodontic MSIs. Materials and Methods: A total of 48 MSIs were placed into bone specimens prepared from the ilium of bovines. Half were placed with the newly designed apparatus and half were placed conventionally. All the specimens were subjected to tangential force loading perpendicular to the MSI with lateral displacement of 0.6 mm, using an Instron Universal Testing machine. The maximum removal torque of each tested specimen was also recorded. Both study and control groups were divided into two subgroups based on whether they had thin and thick cortical bone. Results: The test group had statistically higher force anchorage resistance and maximum insertion torque values than the control group (p < 0.001). The results were found to be more significant in cases in which the cortical bone was thin (p < 0.001). Conclusions: Within the limits of this in vitro study, the present findings suggest that the newly designed apparatus might have a favorable effect on MSI stability in patients presenting with thin cortical bone. Clinical studies are necessary to confirm the results that were observed in vitro.

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