scholarly journals Anti-acne vulgaris effect including skin barrier improvement and 5α-reductase inhibition by tellimagrandin I from Carpinus tschonoskii

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jun Yin ◽  
In Hyoek Hwang ◽  
Min Won Lee
Keyword(s):  
Mrna Expression ◽  
Inhibitory Activity ◽  
Acne Vulgaris ◽  
Skin Barrier ◽  
Positive Control ◽  
Skin Barrier Function ◽  
Rat Liver Microsomes ◽  
Dependent Manner ◽  
Inhibitory Activities ◽  
Anti Inflammatory

Abstract Background Carpinus tschonoskii (CT) has been previously studied for various activities in the improvement of skin diseases. In the present study, we examined the in vitro anti-acne vulgaris (AV) effect of CT leaves (CTL) and tellimagrandin I (TI), one of the main ellagitannins from CT, including skin barrier improvement and 5α-reductase inhibitory activity. Methods To test the anti-AV activities of CTL and TI, firstly, anti-oxidative and anti-inflammatory activities including DPPH radical scavenging activity, nitric oxide (NO) inhibitory activity, and cytokines [interleukin (IL)-6 and IL-8] were tested. Skin barrier improvement experiments were tested using developing cornified envelope (CE) formation, and filaggrin mRNA expression level was determined by RT-PCR. The 5α-reductase inhibitory activity was determined by measuring the testosterone levels in rat liver microsomes. Results CTL and TI showed potent anti-oxidative activity and anti-inflammatory activities. Especially, the cytokine production inhibitory activities of TI were found to be similar to the positive control, epigallocatechin gallate (EGCG). CTL and TI enhanced the CE formation and filaggrin mRNA expression levels and showed potent activities compared to that in the positive control, 1.5 mM Ca2+. In additionally, CTL and TI showed 5α-reductase inhibitory activities in a dose-dependent manner. Conclusion The results showed that CTL and TI inhibit AV endogenous factors such as 5α-reductase and inflammatory cytokines and affect exogenous factors such as developing skin barrier function (CE and filaggrin levels). Therefore, CTL and TI may be plant-derived agent, promising in the treatment of acne vulgaris.

scholarly journals Skin Soothing Effect of Three Herbs from the Namwon-Mt.Jiri Regions

2021 ◽  
Vol 19 (4) ◽  
pp. 595-607
Author(s):  
Jiwon Han ◽  
Bomi Nam ◽  
Seyul Kim ◽  
Yuna Park ◽  
Beom Seok Lee ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Raw Materials ◽  
Radical Scavenging ◽  
Skin Barrier ◽  
Simultaneous Analysis ◽  
Skin Barrier Function ◽  
Expression Levels ◽  
Anti Inflammatory

Purpose: This study was conducted to evaluate lavender, lemongrass, and peppermint grown in the Namwon-Mt.Jiri regions as functional soothing ingredients for cosmetics.Methods: The simultaneous analysis of 19 polyphenols in 50% ethanol-extracted samples of lavender, lemongrass, and peppermint cultivated in the Namwon-Mt.Jiri regions were analyzed by high-performance liquid chromatography (HPLC). Antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Using real-time PCR, improvements in skin barrier function were confirmed by observing the mRNA expression levels of filaggrin and involucrin, and the moisturizing ability was confirmed through the mRNA expression of HAS-2 and HAS-3. The anti-inflammatory efficacy was verified by confirming the expression levels of the inflammatory cytokine IL-6 and the pro-inflammatory mediator nitric oxide (NO).Results: In the simultaneous analysis of 19 phenolic compounds, rosmarinic acid from lavender; chlorogenic acid, syringic acid, p-coumaric acid, and ferulic acid from lemongrass; and caffeic acid, quercitin hydrate, rosmarinic acid, and hesperetin from peppermint were identified. Antioxidant efficacy was confirmed by DPPH radical scavenging, and excellent efficacy was shown in the order of lavender, peppermint, and lemongrass. Filaggrin and involucrin, skin barrier-related genes, were increased more in lavender, lemongrass, and peppermint than in the untreated group. HAS-2 and HAS-3 were also confirmed to be increased in lavender, lemongrass, and peppermint. Lavender, lemongrass, and peppermint all showed concentration-dependent inhibition of IL-6 and NO.Conclusion: Extracts of lavender, lemongrass, and peppermint cultivated in the Namwon-Mt.Jiri regions had excellent antioxidant, skin barrier, moisturizing and anti-inflammatory effects, so may be considered for use as natural raw materials for soothing cosmetics.

scholarly journals Anti-Acne Vulgaris Effects of Pedunculagin from the Leaves of Quercus mongolica by Anti-Inflammatory Activity and 5α-Reductase Inhibition

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2154
Author(s):  
Min Kim ◽  
Jun Yin ◽  
In Hyeok Hwang ◽  
Dong Hui Park ◽  
Eun Kyeong Lee ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Leaf Extract ◽  
Acne Vulgaris ◽  
Positive Control ◽  
No Production ◽  
Quercus Mongolica ◽  
Main Compound ◽  
Inhibitory Activities

Quercus mongolica (QM)—a member of the Fagaceae family—has been used as traditional medicine in Korea, China and Mongolia as a treatment for inflammation of oral, genital or anal mucosa and for external inflammation of skin. To treat acne vulgaris (AV), we evaluated the inhibition of inflammatory cytokines (IL-6 and IL-8) of QM leaf extract (QML) and its main compound, pedunculagin (PD) in vitro and 5α-reductase inhibitory activity by western blotting. As results, QML and PD showed potent NO production inhibitory activity compared with the positive control (PC), NG-monomethyl-L-arginine (L-NMMA). QML and PD was also showed the decreases of IL-6 and IL-8 compared with the PC, EGCG and exhibited potent 5α-reductase type 1 inhibitory activities compared with the PC, dutasteride.

scholarly journals Anti-Inflammatory Effects of Phenolic Compounds Isolated from Quercus Mongolica Fisch. ex Ledeb. on UVB-Irradiated Human Skin Cells

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3094 ◽  
Author(s):  
Jun Yin ◽  
Han Hyuk Kim ◽  
In Hyeok Hwang ◽  
Dong Hee Kim ◽  
Min Won Lee
Keyword(s):  
Mrna Expression ◽  
Janus Kinase ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Monocyte Chemoattractant Protein 1 ◽  
Quercus Mongolica ◽  
Cytokines And Chemokines ◽  
Mitogen Activated Protein ◽  
Anti Inflammatory ◽  
Uvb Exposure

Quercus mongolica Fisch. ex Ledeb. (QM) has been used as an oriental traditional medicine to relieve hemorrhoids, fever, and enteritis. We screened the inhibitory activities of the extracts and compounds (1–6) isolated from QM on the production of inflammatory cytokines and chemokines to evaluate their anti-inflammatory activities. Further, we evaluated the expression levels of cytokines, chemokines, and immune factors on pedunculagin (PC, 1), which was selected from isolated compounds (1–6) because of its potential anti-inflammation effect. Additionally, we evaluated whether the inflammation mitigation effects of PC (1) following UVB exposure in keratinocytes occurred because of nuclear factor (NF)-κB and signal transducer and activator of transcription (STAT)/Janus kinase (JAK) activation. PC (1) remarkably suppressed interleukin (IL)-6, IL-10, IL-13, and monocyte chemoattractant protein-1 (MCP-1) mRNA expression and reduced the mRNA expression level of Cyclooxygenase-2 (COX-2) and also reduced the phosphorylation of p38 mitogen-activated protein kinases (p38), c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) in a concentration-dependent manner.

scholarly journals Increased Anti-Inflammatory Effects on LPS-Induced Microglia Cells by Spirulina maxima Extract from Ultrasonic Process

2019 ◽  
Vol 9 (10) ◽  
pp. 2144 ◽  
Author(s):  
Woon Yong Choi ◽  
Jae-Hun Sim ◽  
Jung-Youl Lee ◽  
Do Hyung Kang ◽  
Hyeon Yong Lee
Keyword(s):  
Mrna Expression ◽  
Dependent Manner ◽  
Spirulina Maxima ◽  
Concentration Dependent Manner ◽  
Quantitative Correlation ◽  
Tnf Α ◽  
Ultrasonic Process ◽  
Anti Inflammatory ◽  
Low Cytotoxicity ◽  
High Concentrations

The Spirulina maxima exact from a non-thermal ultrasonic process (UE) contains 17.5 mg/g of total chlorophyll, compared to 6.24 mg/g of chlorophyll derived from the conventional 70% ethanol extraction at 80 °C for 12 h (EE). The UE also showed relatively low cytotoxicity against murine microglial cells (BV-2) and inhibited the production of the inflammatory mediators, NO and PGE2. The UE also effectively suppresses both mRNA expression and the production of pro-inflammatory cytokines, such as TNF-α, IL-6 and IL-1β, in a concentration-dependent manner. Notably, TNF-α gene and protein production were most strongly down-regulated, while IL-6 was the least affected by all ranges of treatment concentrations. This work first demonstrated a quantitative correlation between mRNA expression and the production of cytokines, showing that suppression of TNF-α gene expression was most significantly correlated with its secretion. These results clearly proved that the anti-inflammatory effects of Spirulina extract from a nonthermal ultrasonic process, which yielded high concentrations of intact forms of chlorophylls, were increased two-fold compared to those of conventional extracts processed at high temperature.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF TINOCRISPOSIDE BY INHIBITING NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDES-STIMULATED RAW 264.7 CELLS

2018 ◽  
Vol 11 (4) ◽  
pp. 149 ◽  
Author(s):  
Adek Zamrud Adnan ◽  
Muhammad Taher ◽  
Tika Afriani ◽  
Annisa Fauzana ◽  
Dewi Imelda Roesma ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Diseases ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Anti Inflammatory

 Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa.Methods: Anti-inflammatory effect was quantified spectrometrically using Griess method by measuring nitric oxide (NO) production after the addition of Griess reagent.Results: The sample concentrations of 1, 5, 25, 50, and 100 μM and 100 μM of dexamethasone (positive control) have been tested against the LPS-stimulated RAW 264.7 cells, and the results showed NO level production of 39.23, 34.00, 28.9, 20.25, 16.3, and 13.68 μM, respectively, and the inhibition level of 22.67, 33.00, 43.03, 60.10, 68.00, and 73%, respectively.Conclusions: From the study, it could be concluded that tinocrisposide was able to inhibit the formation of NO in the LPS-stimulated RAW 264.7 cells in concentration activity-dependent manner, with half-maximal inhibition concentration 46.92 μM. It can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase. The production of NO has been established as a mediator in inflammatory diseases.

scholarly journals HPLC Analysis, Antioxidant, Anti-inflammatory and Xanthine Oxidase Inhibitory Activity of Cudrania tricuspidata

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Shivraj Hariram Nile ◽  
Doo Hwan Kim
Keyword(s):  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Folk Medicine ◽  
Positive Control ◽  
Significant Activity ◽  
Diene Conjugate ◽  
Antioxidant Power ◽  
Cudrania Tricuspidata ◽  
Free Radical Formation ◽  
Anti Inflammatory

Cudrania tricuspidata is a plant used in folk medicine in Korea for treatment of diseases related to oxidative stress and inflammation. In this study the leaf and shoot extract was studied for its antioxidant, anti-inflammatory, and xanthine oxidase (XO) inhibitory activities. The extract with predominant phenolics was quantified using HPLC-DAD. Antioxidant activity was measured using ABTS [2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric ion reducing antioxidant power), and anti-inflammatory activity by diene-conjugate and β-glucuronidase assays. The average antioxidant effects of C. tricuspidata extracts (1–2 mg/mL) revealed significant activity of 32.5% to 50.2% (ABTS) and 24.2 to 40.5% (FRAP) compared with Trolox, having 55.1% (ABTS) and 42.6% (FRAP) activity, respectively. The anti-inflammatory activities showed as 26.5% to 40.5% (DC) and 40.2% to 70.2% (βG) inhibition compared with the control {phenylbutazone; 42.1% (DC) and 80.2% (βG) inhibition, respectively}. The XO inhibitory activity of the plant extract revealed 90.5% inhibition of that of the control (allopurinol) (97% inhibition at 100 μg/mL concentration). The kinetic parameters of XO inhibition revealed a noncompetitive type of inhibition, where, K m and V max of C. tricuspidata extracts (25 to 100 μg/mL) were 0.25 mM/mL and 0.040, 0.036, 0.032, and 0.030 (μg/min), while for the positive control K m and V max the values were 0.30 mM/mL and 0.045 (μg/min), respectively. Results suggest that C. tricuspidata can be exploited against diseases associated with free radical formation and xanthine oxidase activity.

Salazinic Acid-Derived Depsidones and Diphenylethers with α-Glucosidase Inhibitory Activity from the Lichen Parmotrema dilatatum

Planta Medica ◽  
2020 ◽  
Vol 86 (16) ◽  
pp. 1216-1224
Author(s):  
Asshaima Paramita Devi ◽  
Thuc-Huy Duong ◽  
Solenn Ferron ◽  
Mehdi A. Beniddir ◽  
Minh-Hiep Dinh ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Positive Control ◽  
Spectroscopic Analyses ◽  
Ic50 Values ◽  
Inhibitory Activities

AbstractThree new depsidones, parmosidones F – G (1 – 2), and 8′-O-methylsalazinic acid (3), and 3 new diphenylethers, parmetherines A – C (4 – 6), together with 2 known congeners were isolated from the whole thalli of Parmotrema dilatatum, a foliose chlorolichen. Their structures were unambiguously determined by extensive spectroscopic analyses and comparison with literature data. The isolated polyphenolics were assayed for their α-glucosidase inhibitory activities. Newly reported benzylated depsidones 1 and 2 in particular inhibited α-glucosidase with IC50 values of 2.2 and 4.3 µM, respectively, and are thus more potent than the positive control, acarbose.

scholarly journals Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells

Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2706 ◽  
Author(s):  
Akshay Bisht ◽  
Martin Dickens ◽  
Kay Rutherfurd-Markwick ◽  
Rohith Thota ◽  
Anthony N. Mutukumira ◽  
...  
Keyword(s):  
Cell Viability ◽  
Chlorogenic Acid ◽  
Mrna Expression ◽  
Inflammatory Marker ◽  
Pcr Analysis ◽  
Marker Genes ◽  
Dependent Manner ◽  
Major Barrier ◽  
Tnf Α ◽  
Anti Inflammatory

The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.

scholarly journals Phenolic Constituents from the Heartwood of Artocapus Altilis and their Tyrosinase Inhibitory Activity

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Mai Ha Khoa Nguyen ◽  
Hai Xuan Nguyen ◽  
Mai Thanh Thi Nguyen ◽  
Nhan Trung Nguyen
Keyword(s):  
Phenolic Compounds ◽  
Vanillic Acid ◽  
Inhibitory Activity ◽  
Kojic Acid ◽  
Positive Control ◽  
Coumaric Acid ◽  
Meoh Extract ◽  
Tyrosinase Inhibitory Activity ◽  
Phenolic Constituents ◽  
Inhibitory Activities

From the MeOH extract of the heartwood of Artocapus altilis, thirteen phenolic compounds have been isolated, namely curcumin (1), desmethoxycurcumin (2), retrodihydrochalcone (3), apigenin (4), tangeretin (5), nobiletin (6), O-methyldehydrodieugenol (7), dehydrodieugenol (8), β-hydroxypropiovanillone (9), p-coumaric acid (10), p-hydroxybenzaldehyde (11), vanillin (12), and vanillic acid (13). This is the first report on the presence of these compounds in the heartwood of A. altilis. Compounds 1, 2, and 10 showed more potent tyrosinase inhibitory activities, with IC50 values ranging from 2.3 to 42.0 μM, than the positive control kojic acid (IC50, 44.6 μM). The most active compound, p-coumaric acid (10) (IC50, 2.3 μM), was 22 times more active in tyrosinase inhibitory activity than kojic acid.

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