scholarly journals Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Xiaolin Li ◽  
Hongli Jing ◽  
Xiaofei Liu ◽  
Qin Wang ◽  
Songyin Qiu ◽  
...  
Keyword(s):  
Test Performance ◽  
Comparative Evaluation ◽  
Diagnostic Technique ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Crossings ◽  
Akabane Virus ◽  
Elisa Kit ◽  
Inspection And Quarantine ◽  
Surveillance Programs ◽  
Cattle Serum

Abstract Background Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a commonly used diagnostic technique for AKAV infection, including the IDEXX and IDVET ELISA kits. However, the comparative evaluation of the IDEXX and IDVET ELISA kits has not been published. The object of this study was to evaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle. Results With virus neutralization test (VNT) as the “relative gold standard”, the diagnostic sensitivity (DSe) was 80.39% (123/153) and 93.46% (143/153) for the IDEXX and IDVET ELISA kit, when suspect samples were included. The diagnostic specificity (DSp) for the IDEXX and IDVET ELISA kit was 93.48% (502/537) and 82.31% (442/537), respectively. Conclusion Both of the tested ELISA kits could be applied to detect antibodies against AKAV in cattle serum. The IDVET ELISA kit had a higher DSe. The IDEXX ELISA kit possessed the higher DSp. These results have important implications if the kits are used to screen herds or individual cattle in surveillance programs, or at border crossings for import-export inspection and quarantine.

scholarly journals Performance analysis of rapid diagnostic tests on atypical bovine spongiform encephalopathy

2012 ◽  
Vol 24 (5) ◽  
pp. 976-980 ◽  
Author(s):  
John G. Gray ◽  
Sandor Dudas ◽  
Catherine Graham ◽  
Stefanie Czub
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Test Performance ◽  
Rapid Test ◽  
Rapid Diagnostic Tests ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spongiform Encephalopathy ◽  
Analytical Sensitivity ◽  
Atypical Bovine Spongiform Encephalopathy ◽  
Surveillance Programs ◽  
Rapid Tests

The preferred method to determine the prevalence of bovine spongiform encephalopathy (BSE) in a country is to use immunology-based rapid-tests. Though these tests are validated to detect C-type BSE disease–associated prion (PrPsc), test-specific properties may influence their ability to detect H- and/or L-type BSE PrPsc, where both are atypical from C-type PrPsc. Molecular characterization shows atypical BSE PrPsc to have a different sensitivity to proteinase activity and different affinities for certain prion-specific antibodies. It is important to understand how atypical BSE PrPsc may affect the performance of rapid-tests, which are typically dependant on the use of specific proteases and antibodies. The current study used experimentally generated C-, H-, and L-type BSE PrPsc to evaluate 3 tests used in various national BSE surveillance programs: an immunochromatographic assay, a standard sandwich enzyme-linked immunosorbent assay (stndELISA), and a PrPsc-conformation–specific ELISA (confELISA). Although BSE PrPsc type had some effects on rapid-test performance, analytical sensitivity for atypical BSE PrPsc on all 3 platforms was not significantly compromised. When testing for atypical BSE PrPsc, the 3 tests were able to meet the same requirements that the European Food Safety Authority set when evaluating the tests for C-type BSE PrPsc.

scholarly journals Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis

2021 ◽  
Vol 14 (3) ◽  
pp. 803-812
Author(s):  
Mohandoss Nagalingam ◽  
Thaslim J. Basheer ◽  
Vinayagamurthy Balamurugan ◽  
Rajeswari Shome ◽  
S. Sowjanya Kumari ◽  
...  
Keyword(s):  
Western Blot ◽  
Recombinant Proteins ◽  
Brucella Abortus ◽  
Comparative Evaluation ◽  
Area Under The Curve ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Diagnostic Potential ◽  
Cattle Serum

Background and Aim: The present serodiagnosis of brucellosis in livestock is based on the whole cell or smooth lipopolysaccharide of the Brucella organism in which specificity is hampered by the cross-reactivity, especially with the antibodies against Yersinia enterocolitica O:9 organism. The problem can be addressed by screening for better immunodominant antigens. Hence, the present study was undertaken to screen protein antigens of Brucella abortus for their diagnostic potential in cattle brucellosis. Materials and Methods: Protein antigens of B. abortus (n=10) non-reactive to antibodies against Y. enterocolitica O:9 were selected, expressed in Escherichia coli, assessed the reactivity of expressed recombinant proteins by Western blot, standardized indirect-enzyme-linked immunosorbent assay (ELISA) for detecting Brucella antibodies in cattle serum, and comparative evaluation was done. Results: All the selected protein antigens were expressed and in the Western blot with Brucella antibodies positive cattle serum, six recombinant (Brucella protein 26 [BP26], Cu-Zn Superoxide dismutase [SodC], B. abortus I-1885, Serine protease, Bacterioferritin, and Brucella Lumazine Synthase [BLS]) proteins showed reaction whereas none of the proteins showed reactivity with Brucella negative cattle serum. ELISA has been done using known Brucella positive and negative cattle sera samples (n=113 each) in which the performance of recombinant proteins in diagnosing brucellosis was in the order of BP26 > BLS > SodC followed by rest of the proteins. BP26 based ELISA was found to be better with area under the curve as 0.953, and diagnostic sensitivity, diagnostic specificity, and Youden's index of 90.27%, 95.58%, and 0.8584, respectively, with the excellent agreement (k=0.85). Conclusion: BP26 could be a potential diagnostic antigen among the immunodominant proteins of B. abortus in ruling out Y. enterocolitica O:9 infection while diagnosing brucellosis in cattle herds.

Can Osteopontin Be Considered a Biomarker for Endometriosis?

2017 ◽  
Vol 68 (9) ◽  
pp. 2132-2134
Author(s):  
Daniela Roxana Albu (Matasariu) ◽  
Elena Mihalceanu ◽  
Alina Pangal ◽  
Carmen Vulpoi ◽  
Mircea Onofriescu ◽  
...  
Keyword(s):  
Serum Level ◽  
Immunosorbent Assay ◽  
Pelvic Pain ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Multifactorial Disease ◽  
Elisa Kit ◽  
Healthy Women ◽  
Progesterone Treatment ◽  
Large Dispersion

Endometriosis is a multifactorial disease that is manifested by infertility and pelvic pain. The purpose of the study was to evaluate the effect of progesterone treatment on the serum level of osteopontin, a multipotent cytokine, in patients with endometriosis. The study was prospective and we evaluated osteopontin levels that were measured in the serum of 40 patients with endometriosis and 12 healthy women using a standardized Enzyme-Linked Immunosorbent Assay (ELISA) kit. Osteopontin seric levels were lower in endometriosis patients and increased after progesterone treatment. Because of the large dispersion of data even in the control group, we find the association between osteopontin and endometriosis questionable.

Detection of antibodies against akabane virus in bovine sera by enzyme-linked immunosorbent assay

1989 ◽  
Vol 20 (3) ◽  
pp. 275-280 ◽  
Author(s):  
S. Ide ◽  
K. Baba ◽  
M. Tsuchimoto ◽  
H. Nagano ◽  
Y. Eiguchi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Akabane Virus

scholarly journals Evaluation of an enzyme immunoassay for clinical diagnosis of neurocysticercosis in symptomatic patients

2010 ◽  
Vol 43 (6) ◽  
pp. 647-650 ◽  
Author(s):  
Reynaldo Mendes de Carvalho Junior ◽  
Dorcas Lamounier Costa ◽  
Savyo Carvalho Soares ◽  
Carlos Henrique Nery Costa
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Sensitivity And Specificity ◽  
Cerebral Spinal Fluid ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Disease ◽  
Multivariate Logistic Regression ◽  
Human Central Nervous System ◽  
Elisa Kit

INTRODUCTION: Neurocysticercosis is an infection of the human central nervous system caused by the metacestode larvae of Taenia solium. Neurocysticercosis is the most common parasitic disease in developing countries. Epilepsy is the most common clinical manifestation. Difficulties in confirming the diagnosis motivated the evaluation of the enzyme-linked immunosorbent assay on cerebral spinal fluid (CSF). METHODS: Twenty-two patients with NCC and 44 control patients were studied. CSF was analyzed using a commercial ELISA kit developed for NCC. Sensitivity and specificity were measured and a multivariate logistic regression was performed. RESULTS: Sensitivity and specificity of ELISA were 31.8% and 100%, respectively, with accuracy of 77.3%. Only the size of the lesions proved to be important for performance of the test. CONCLUSIONS: The results showed that ELISA contributes to the diagnosis of neurocysticercosis if the result is negative or if the patient has a lesion of 2 cm or more.

Comparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogs

2008 ◽  
Vol 157 (3-4) ◽  
pp. 175-181 ◽  
Author(s):  
Teresinha Cristina Cândido ◽  
Sílvia Helena Venturoli Perri ◽  
Tatiana de Oliveira Gerzoschkwitz ◽  
Maria Cecília Rui Luvizotto ◽  
Valéria Marçal Felix de Lima
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Comparative Evaluation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Purified Antigen

scholarly journals Kadar Prostaglandin dan Endorfin pada Remaja dengan Dismenore Primer yang diberi Hidroterapi Hangat dan Dingin

2020 ◽  
Vol 12 (1) ◽  
pp. 115-121
Author(s):  
Andi Asrina ◽  
Arsyad Aryadi ◽  
Nilawati Andi
Keyword(s):  
Experimental Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Primary Dysmenorrhea ◽  
Not Given ◽  
Elisa Kit ◽  
Group Design ◽  
Prostaglandin Level ◽  
Post Test ◽  
Quasi Experimental ◽  
The Mean

This study aims to determine the comparison of prostaglandin and endorphin levels in adolescents with primary dysmenorrhea with and without warm (37-40oC) and cold (18-20oC) hydrotherapy. This quasi-experimental study with a post-test only controls group design was carried out in Islamic Boarding Schools with a sample of 36 young girls divided into 3 groups: 12 teens given warm hydrotherapy, 12 teens given cold hydrotherapy and 12 teens not given intervention (control). Blood plasma is taken after an intervention is given on the first day of menstruation. Examination of prostaglandin and endorphins levels using the enzyme-linked immunosorbent assay (ELISA) kit method. After cold hydrotherapy, the mean levels of prostaglandins in the cold hydrotherapy group were twice higher (569 pg/ml) compared to controls (394 pg/ml). The mean prostaglandin level in the warm hydrotherapy group also showed an increase prostaglandin (437 pg/ml) compared to the control (394 pg/ml). In addition to increasing levels of prostaglandins, increased levels of endorphins also occurred in the group given warm hydrotherapy (154 pg/ml) and the group was given cold hydrotherapy (187 pg/ml) compared to the control (119 pg/ml) p = 0.001. The conclusion in this study is that warm and cold hydrotherapy can increase levels of prostaglandins and endorphins in adolescents with primary dysmenorrhea. However, cold hydrotherapy increases endorphin levels higher than warm hydrotherapy. Key words: Prostaglandin, Endorphin, Hydrotherapy, Primary Dismenorrhea.

scholarly journals High Anti-HCV Seroprevalence and Low Performance of ICT Strip in Diagnosing Hepatitis C Virus Infection in Children in Enugu Metropolis

2018 ◽  
Vol 12 (1) ◽  
pp. 149-156
Author(s):  
Maryann C. Ezeilo ◽  
Godwill A. Engwa ◽  
Romanus I. Iroha ◽  
Damian N. Odimegwu
Keyword(s):  
Risk Factors ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Test Performance ◽  
Clinical Manifestations ◽  
Signs And Symptoms ◽  
Cross Sectional Study ◽  
Hcv Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional

Background:The lack of a vaccine for Hepatitis C virus (HCV) places children at a high risk of contracting the infection. It becomes necessary to accurately diagnose this infection for proper treatment as well as identifying potential risk factors for effective management.Aim:This study was conceived to assess the test performance of the commonly used Immunochromatographic test (ICT) strip and identify the associated clinical manifestations and risk factors of HCV in children in Enugu Metropolis.Method:A cross-sectional study involving randomly selected 270 children below six years of age was conducted in Enugu Nigeria. The subjects were screened for anti-HCV by ICT and Enzyme-Linked Immunosorbent Assay (ELISA) and the demographic, signs and symptoms and risk factors were collected.Results:A total of 50 out of 270 children were positive for anti-HCV with a seropositivity of 18.5%. ICT strip had a very low sensitivity of 38.00% with an accuracy of 88.52% in detecting anti-HCV. The presence of dark urine was associated (p= 0.01) with HCV infection.Conclusion:A seroprevalence of 18.5% of Anti-HCV was found in children below six years old in Enugu metropolis and the performance of ICT in diagnosing HCV infection was poor compared to ELISA.

Development of Direct Competitive ELISA Kit for the Detection of Tetrodotoxin Using HRP Labeled Antigen

2011 ◽  
Vol 236-238 ◽  
pp. 2820-2824 ◽  
Author(s):  
Qing Ping Zhong ◽  
An Cheng Huang ◽  
Bin Wang ◽  
Xue Dong
Keyword(s):  
Room Temperature ◽  
Inhibitory Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Competitive Reaction ◽  
Antigen Concentration ◽  
Time Saving ◽  
Elisa Kit ◽  
Standard Curve ◽  
Coating Method ◽  
Ml Detection

The direct competitive enzyme-linked immunosorbent assay (dcELISA) kit was developed for detecting tetrodotoxin (TTX). The working conditions of the dcELISA kit including the anti-TTX mAb coating concentration, coating method, enzyme-labeled antigen concentration, the antigen diluents, reaction time and temperature were all optimized. The result showed that mAb coating concentration was 3.72 μg/ml, it was coated at the condition of minimal power treatment of microwave oven for 3 min. The enzyme-labeled antigen concentration was 4.08 μg/ml. The competitive reaction was under the condition of room temperature 25 °C for 30 min. The half maximal inhibitory concentration (IC50) of the standard curve was 20.4 ng/ml, detection limit was 1.1 ng/ml, linear range 3.3～137 ng/ml, the intra-assay CV and inter-assay CV were 6.25% and 7.34% respectively. And recovery rate of TTX ranged from 65.0% to 93.2% with the CV of 9.41～12.77%. This method is convenient, sensitive and time-saving, hope this dcELISA kit can bring benefits and reference for TTX detection.

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