The protective effect of Jangkanghwan (Korean traditional food) on lipopolysaccharide-induced disruption of the colonic epithelial barrier

AbstractJangkanghwan (JKH) is a Korean traditional food that is a mixture of food ingredients and traditional Korean medicine ingredients, and it has been observed to produce satisfactory anti-inflammatory, antioxidant, and antibacterial effects. In the current study, JKH was administered by gavage to BALB/C mice with lipopolysaccharide (LPS)-induced colonic epithelial dysfunction, and mouse body weight and food intake were recorded. Indexes such as colonic paracellular permeability, serum inflammatory cytokines, and bacterial translocation were used to comprehensively evaluate the regulatory effect of JKH on mouse colonic epithelial function, and qPCR and Western blot were also used to analyze the expression of tight junction (TJ)-related genes, such as occludin, claudin, zonula occludens (ZOs) proteins, and junction adhesion molecules (JAM) in the colonic epithelial tissue. The experimental results indicated that JKH relieved the edema of the liver, spleen, and mesenteric lymph node tissues, and reduced the loss of appetite and diarrhea caused by LPS injection in mice. It increased the amount of mice food intake from 3.7 g/day in the LPS group to 4.7 g per day; the water content in the feces of mice in the JKH group was 13.86% less than that in the LPS group. JKH reduced the inflammatory response in mice caused by LPS, protected the integrity of the colon, the permeability of fluorescent macromolecules was one-fourth of the LPS group, and enhanced the mRNA and protein expression of TJ-related proteins in colon tissue. Our findings highlight that JKH has benefits in intestinal health and relieving systemic inflammation, relevant aspects of its use as a functional food.

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Establishment of an Experimental Breast Cancer ZHENG Model and Curative Effect Evaluation of Zuo-Jin Wan

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/324732 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Jia Du ◽

Yang Sun ◽

Xiu-Feng Wang ◽

Yi-Yu Lu ◽

Qian-Mei Zhou ◽

...

Keyword(s):

Breast Cancer ◽

Body Weight ◽

Food Intake ◽

The Body ◽

Effect Evaluation ◽

Curative Effect ◽

Liver Functions ◽

Mouse Breast Cancer ◽

Loss Of Appetite ◽

Tcm Syndrome

Herbal formulas based on the traditional Chinese medicine (TCM) syndrome (ZHENG) have been used as alternative treatments for breast cancer. However, there is a lack of the experimental animal ZHENG model for the evaluation of the herbal formulas. In this study, we have established 4T1 mouse breast cancer with Liver Fire Invading Stomach Syndrome model (4T1 LFISS mice) and investigated the effects of the herbal formula, Zuo-Jin Wan (ZJW). Our results showed that 4T1 LFISS mice have the features of LFISS including irritability, loss of appetite, yellow urine, chow, and a tail hot. Compared to untreated 4T1 LFISS mice, ZJW significantly reduced tumor weight and volume (P<0.05), although it was weaker than Cisplatin. However, ZJW significantly increased the body weight and food intake of 4T1 LFISS mice and decreased serum ALT, AST, Cr, and BUN levels and ZHENG score (P<0.05), while Cisplatin reduced the food intake, and body weight and increased serum ALT, AST, Cr, and BUN levels in 4T1 LFISS mice. Our study has provided a mouse breast cancer ZHENG model and showed that ZJW suppresses tumor growth and improves LFISS and kidney and liver functions in the 4T1 LFISS mice.

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Agave americana (Ketuke) toxicity in the kidney of Clarias batrachus

Journal of Natural History Museum ◽

10.3126/jnhm.v30i0.27573 ◽

2018 ◽

Vol 30 ◽

pp. 244-247

Author(s):

Archana Prasad ◽

Rukmini Puri

Keyword(s):

Behavioral Change ◽

The Other ◽

Clarias Batrachus ◽

Epithelial Tissue ◽

Renal Tubules ◽

Histopathological Alterations ◽

Agave Americana ◽

Excessive Secretion ◽

Loss Of Appetite ◽

Different Parts

Different parts of plant have been used since many decades by humans for catching fishes. In this investigation, ‘ketuke ‘leaf effect was observed on behavioral change and histological alterations in the kidney of Clarias batrachus. Few fishes were kept as control i.e. not exposed to plant piscicide. The other fishes were exposed to 3gm per 30 liters of water for 24 and 48 hrs. In exposed fish’s restlessness, vigorous movement, excessive secretion of slime and loss of appetite was seen. Histologically, glomerular contraction, clumping of melanomacrophages, degeneration of epithelial tissue along with the degeneration of renal tubules and others were seen. Increase in the Bowman’s space was one of the noticeable changes. Extract accumulation was seen in the tissues. Exposure to sub lethal concentrations of Ketuke (Agave americana), caused duration dependent histopathological alterations in the kidney of Clarias batrachus which is discussed in the paper.

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Creating bespoke note by note dishes and drinks inspired by traditional foods

Journal of Ethnic Foods ◽

10.1186/s42779-020-00071-3 ◽

2020 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Róisín M. Burke ◽

Pauline Danaher ◽

David Hurley

Keyword(s):

Nutritional Value ◽

Traditional Food ◽

Traditional Foods ◽

Food Ingredients ◽

Food Neophobia ◽

Molecular Gastronomy ◽

International Activities ◽

The Creation

Abstract French physical chemist, Hervé This, first proposed Note by Note (NbN) cooking in 1994. It is an application of molecular gastronomy and involves the use of compounds, either pure or in mixtures, rather than traditional food ingredients to make dishes. A review of international activities, relating to NbN cooking and cuisine, reveals that it has the potential to contribute to the creation of bespoke foods and drinks, e.g., by using ingredients which are sustainable and which provide nutritional value. However the ‘food neophobia’ of some consumers needs to be overcome to ensure its success. In light of these findings, it was decided to explore if and how the factors of food neophobia, sustainability and nourishment influenced students when they were developing their NbN dishes/drinks for their module assignment in TU Dublin. One hundred and five students at TU Dublin have, since 2013, completed NbN assignments and their corresponding reports which provide details of the development of their dishes/drinks. Eighty-seven of these were available for analysis. It was found that the majority of the students (62%) were inspired by traditional foods to create bespoke NbN dishes/drinks, helping to address the ‘food neophobia’ of some consumers. The development of one of these dishes and one of these drinks is discussed. This information would be beneficial for others who will be developing NbN dishes/drinks.

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Prophylactic Effect of Lactobacillus plantarum YS4 on Oxazolone-Induced Colitis in BALB/c Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/9048971 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Ruokun Yi ◽

Fang Tan ◽

Huayi Suo ◽

Wenfeng Li ◽

Xianrong Zhou ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Serum Levels ◽

Lactobacillus Bulgaricus ◽

Negative Effects ◽

Colon Tissue ◽

Mouse Colon ◽

Mrna And Protein Expression ◽

The Common ◽

Commercial Kits ◽

Better Than

In the present research, the effects of Lactobacillus plantarum YS4 (LP-YS4) on colitis were tested in an oxazolone-induced mouse model. BALB/c mice were induced by oxazolone and then treated with LP-YS4. The serum levels of mice were analyzed using commercial kits and the protein and mRNA expression levels of mouse colon tissue were detected by Western blotting and qPCR assay, respectively. The results demonstrated that LP-YS4 significantly (P<0.05) increased the colon length and ratio of colon weight/length in mice with colitis and attenuated the negative effects of colitis. The results also showed that treatment with LP-YS4 significantly reduced the serum concentrations of ET-1, SP, and IL-10 while significantly increasing those of SS, VIP, and IL-2 in colitis mice (P<0.05). In addition, LP-YS4 significantly increased the activities of GSH and SOD while decreasing those of MPO and MDA in the colon tissue of colitis mice (P<0.05). LP-YS4 also significantly upregulated the mRNA and protein expression of c-Kit, eNOS, nNOSe, and SCF in colitis mice while significantly downregulating the relative expression of iNOS. In summary, LP-YS4 could reduce the negative effects of colitis, and such effects were better than those of the common probiotic Lactobacillus bulgaricus.

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The mitochondrial-dependent pathway is chronically affected in testicular germ cell death in adult rats exposed in utero to anti-androgens

Journal of Endocrinology ◽

10.1677/joe.1.05771 ◽

2004 ◽

Vol 183 (1) ◽

pp. 79-90 ◽

Cited By ~ 28

Author(s):

A Bozec ◽

F Chuzel ◽

S Chater ◽

C Paulin ◽

R Bars ◽

...

Keyword(s):

Germ Cells ◽

Sertoli Cells ◽

In Utero ◽

Testicular Germ Cell ◽

Adult Rats ◽

Wide Range ◽

Mrna And Protein Expression ◽

Related Proteins ◽

Dependent Pathway

In utero exposure to exogenous anti-androgenic compounds induces a wide range of abnormalities of the reproductive system, including hypospermatogenesis, cryptorchidism and hypospadias. By using rats exposed in utero to the anti-androgenic compound flutamide (0.4, 2 or 10 mg/kg per day), it has been shown that hypospermatogenesis in adult testes could be related to (i) a long-term apoptosis in germ cells but not in somatic Leydig and Sertoli cells as evidenced by the TUNEL approach and (ii) alterations in the mRNA and protein expression of pro- (Bax, Bak, Bid) and anti-apoptotic (Bcl-2, Bcl-w) members of the Bcl-2 family. Indeed, the number of apoptotic germ cells increased with the dose of flutamide administered and the apoptotic germ cells were mainly detected at androgen-dependent stages VII–VIII. Moreover, for the Bcl-2-related proteins that were expressed mainly in the germ cells, a decrease in the levels of anti-apoptotic peptides Bcl-w (60%, P=0.003) and Bcl-2 (90%, P=0.0001) was observed at 2 mg/kg per day flutamide and an increase in levels of the pro-apoptotic Bax (2.3-fold, P=0.0004) was detected at 10 mg/kg per day. In contrast, the levels of pro-apoptotic peptide Bak that was mainly expressed in somatic cells decreased (70%, P=0.0008) at 10 mg/kg per day. Such alterations in Bcl-2-related peptides occurred mainly at the protein level except for Bcl-2 (72%, P=0.0001) and Bak (43%, P=00002) transcripts. Together, these results showed that the apoptosis observed in adult germ cells from rats exposed in utero to flutamide may result from a long-term alteration in the balance between pro- and anti-apoptotic Bcl-2-related molecules in favour of pro-apoptotic proteins. These data further supported the concept of an androgen-dependent fetal programming that is in relation with an alteration of the expression of Bcl-2-related genes/proteins promoting apoptosis in testicular germ cells of adult rats with fetal androgen disruption.

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Antibacterial effect of traditional food ingredients for healthcare on Helicobacter pylori

Technology and Health Care ◽

10.3233/thc-191735 ◽

2019 ◽

Vol 27 (5) ◽

pp. 509-518 ◽

Cited By ~ 2

Author(s):

Jae-Hyeok Lee ◽

Jeong-Sook Park

Keyword(s):

Helicobacter Pylori ◽

Antibacterial Effect ◽

Traditional Food ◽

Food Ingredients

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Expression of Glycogenic Genes in the Oviduct of Chinese Brown Frog (Rana Dybowskii) During Pre-Hibernation

10.21203/rs.3.rs-829182/v1 ◽

2021 ◽

Author(s):

Chang Fan ◽

Yuning Liu ◽

Ao Zhang ◽

Wenqian Xie ◽

Haolin Zhang ◽

...

Keyword(s):

Breeding Season ◽

Glucose Transporter ◽

Glycogen Synthesis ◽

Mrna Levels ◽

Morphological Studies ◽

Mrna And Protein Expression ◽

Brown Frog ◽

Gsk 3Β ◽

Related Proteins ◽

Rana Dybowskii

Abstract Background: The oviduct of Chinese brown frog (Rana dybowskii) expands specifically during pre-hibernation. However, the underlying molecular mechanism remains unclear. Results: This study investigated the mRNA and protein expression levels of glycogenic genes in the oviduct of Rana dybowskii during the breeding season and pre-hibernation. Morphological studies revealed increased weight and enlarged diameter of the oviduct in pre-hibernation. Furthermore, significantly increased glycogen level was detected in the oviduct during pre-hibernation when compared to the breeding season. Transcriptome analysis further identified that the differentially expressed genes in the synthesis and metabolism pathways of carbohydrates in the oviduct during pre-hibernation. The relative mRNA levels of glycolysis and glycogenesis-related genes were up-regulated, while gluconeogenesis-related genes were down-regulated during pre-hibernation. Also, Western blot showed that glucose transporter and glycogen synthesis-related proteins, such as total-GYS, and p-GSK-3β were highly expressed in the oviduct during pre-hibernation. In addition, immunohistochemical data showed that glucose transporter and total-GYS, p-GYS, total-GSK-3β and p-GSK-3β related to glycogen synthesis were both expressed regionally in the oviduct of Rana dybowskii during the breeding season and pre-hibernation. Conclusions: The data suggests that carbohydrate metabolism plays an important role in the hibernation process of Rana dybowskii, and provides a new insight into the mechanism underlying the expansion of the oviduct of Rana dybowskii during pre-hibernation.

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SOME DIETARY AND HORMONAL EFFECTS ON MATERNAL, FOETAL AND PLACENTAL WEIGHTS IN THE RAT

Journal of Endocrinology ◽

10.1677/joe.0.0090068 ◽

1953 ◽

Vol 9 (1) ◽

pp. 68-75 ◽

Cited By ~ 22

Author(s):

ROSA M. CAMPBELL ◽

I. R. INNES ◽

H. W. KOSTERLITZ

Keyword(s):

Body Weight ◽

Food Intake ◽

Negative Correlation ◽

Favourable Effect ◽

Stomach Tube ◽

Maternal Body ◽

Hypophysectomized Rats ◽

The Mean ◽

Loss Of Appetite ◽

Definite Correlation

1. A negative correlation was found between the mean weight of an individual placenta and the number in a litter. Within the range examined and with the methods employed, no definite correlation between the mean weight of an individual foetus and litter size was established. 2. Reduction of the food intake to one-half or one-third during the last 6 days of pregnancy did not result in a decrease in the number and weight of foetuses and placentae. This was apparently brought about by drawing on the maternal reserves laid down during the first 14 days of pregnancy. Absence of protein from the diet during the last 6 days resulted in a very moderate decrease in foetal and placental weights. 3. Absence of the maternal adrenals or pituitary had no influence on foetal and placental weights. The decrease in maternal body weight after hypophysectomy was solely due to loss of appetite and could be prevented by giving the rats adequate amounts of food by stomach tube. 4. The increase in body weight during the first 14 days of pregnancy was maintained after removal of the foetuses as long as the placentae survived. The placentae also had a favourable effect on the maternal body weight in the absence of the foetuses, when the ovaries or adrenals or the pituitary or the pituitary and ovaries were removed, but not when both ovaries and adrenals were absent. The placental factor responsible for this effect appears to act through the ovaries or adrenals. 5. The increase in the weight of the ovaries during pregnancy was not affected by removal of the foetuses, foetuses and pituitary, or foetuses and adrenals. The adrenals of pregnant hypophysectomized rats were not heavier than those of non-pregnant hypophysectomized rats.

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The Modernization of Malay Food Ingredients: A qualitative investigation

Environment-Behaviour Proceedings Journal ◽

10.21834/e-bpj.v1i3.367 ◽

2016 ◽

Vol 1 (3) ◽

pp. 227

Author(s):

Mohd. Shazali Md. Sharif ◽

Norazmir Md. Nor ◽

Ahmad Esa Abdul Rahman ◽

Khairunnisa Mohamad Abdullah

Keyword(s):

Open Access ◽

Qualitative Method ◽

Publishing House ◽

Traditional Food ◽

Qualitative Investigation ◽

Preparation Time ◽

Food Ingredients ◽

International Publishing ◽

Insight Into ◽

Open Access Article

This paper attempts to provide and insight into the modernization of Malay food ingredients within Malay communities. Its approach is sociological, using qualitative method of interview and observation, inquiring into the usage of modern food ingredients in the preparation of Malay dishes. Focusing on a small group of consumers in Puncak Alam, Selangor, the findings indicate that the motivation behind choosing modern food ingredients is to save cooking preparation time while improving nutrient intakes.© 2016. The Authors. Published for AMER ABRA by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.Keywords: modernizations; traditional food; Malay food; food ingredients

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