scholarly journals Vinasse: from a residue to a high added value biopolymer

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Daiana V. Trapé ◽  
Olivia V. López ◽  
Marcelo A. Villar
Keyword(s):  
Culture Medium ◽  
Added Value ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Response Variable ◽  
Independent Variables ◽  
Lower Productivity ◽  
Phb Production ◽  
Feasible Alternative ◽  
Growing Conditions

AbstractThis work aimed to study the feasibility of using vinasse for polyhydroxybutyrate (PHB) production by Bacillus megaterium. To optimize the culture medium, a Box–Behnken design was employed considering carbon (C), nitrogen (N), and phosphorus (Ph) concentrations as independent variables and PHB productivity as the response variable. The productivity decreased when C or N were increased, probably due to the presence of phenolic compounds and the limitation of N for the production of PHB by Bacillus sp. bacteria. An additional experimental design to optimize the C/N ratio and growing conditions (fermentation time and temperature) was carried out. Fermentation time had a statistically significant effect on PHB productivity reaching 10.6 mg/L h. On the other hand, the variability in physicochemical properties of vinasse samples led to significant differences in PHB productivity. Lower productivity values were obtained when vinasse had higher values of DBO. Therefore, biopolymers production from vinasse is a feasible alternative to valorize this bioethanol by-product. Graphical Abstract

scholarly journals Vinasse: From A Residue To A High Added Value Biopolymer

2021 ◽  
Author(s):  
Daiana Trapé ◽  
Olivia V. López ◽  
Marcelo A. Villar
Keyword(s):  
Culture Medium ◽  
Added Value ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Response Variable ◽  
Independent Variables ◽  
Lower Productivity ◽  
Phb Production ◽  
Feasible Alternative ◽  
Growing Conditions

Abstract This work aimed to study the feasibility to use vinasse for polyhydroxybutyrate (PHB) production by Bacillus megaterium. To optimize the culture medium, a Box Behnken design was employed considering carbon (C), nitrogen (N), and phosphorus (Ph) concentrations as independent variables and PHB productivity as the response variable. The productivity decreased when C or N were increased, probably due to the presence of phenolic compounds and the limitation of N for the production of PHB by Bacillus sp. bacteria. An additional experimental design to optimize the C/N ratio and growing conditions (fermentation time and temperature) was carried out. Fermentation time had a statistically significant effect on PHB productivity reaching 10.6 mg/L h. On the other hand, the variability in physicochemical properties of vinasse samples led to significant differences in PHB productivity. Lower productivity values were obtained when vinasse had DBO high values. Therefore, biopolymers production from vinasse is a feasible alternative to valorize this bioethanol by-product.

scholarly journals PRELIMINARY STUDIES FOR PRODUCING CRUDE LIPASE FROM TEMPE’S MOULD CULTIVATED IN RICE-HUSK-BASED SOLID MEDIA

2010 ◽  
Vol 7 (2) ◽  
pp. 223-229
Author(s):  
Teuku Beuna Bardant ◽  
Kiky Corneliasari Sembiring ◽  
Achmad Hanafi Setiawan
Keyword(s):  
Rice Husk ◽  
Palm Oil ◽  
Coconut Oil ◽  
Added Value ◽  
Media Composition ◽  
Fermentation Time ◽  
Solid Media ◽  
Oil Derivatives ◽  
Solid Fermentation ◽  
Hydrolysis Activity

The goal of these preliminary studies is to support Indonesian program for increasing palm oil added value through independent production technology based on Indonesian natural resources. Various palm oil derivatives could be synthesized enzymatically using lipase from microbes that available in Indonesia. Tempe's mould is available in abundance in Indonesia and had already been proved for producing lipase. This paper provides information about producing crude lipase from Tempe's mould cultivated in rice-husk-based solid media using palm oil as carbon source. Observed variables include solid media composition, optimum fermentation time, extraction and enriching process of crude lipase. The crude lipase was analyzed its hydrolysis activity on coconut oil and palm oil. The result of these preliminary studies shows that this production process is a simple and tough process and very potential to be developed.   Keywords: lipase, Tempe's mould, palm oil, solid fermentation, rice husk

A Novel Application of Refinery Merox Unit Wastewater for Bovine Hide Unhairing

2020 ◽  
Vol 115 (8) ◽  
pp. 301-308
Author(s):  
Asgarifard Pourya ◽  
Tafreshi Navid ◽  
Sharifi Akbar
Keyword(s):  
Optimum Condition ◽  
Polynomial Equation ◽  
Process Time ◽  
P Value ◽  
Environment Pollution ◽  
Box Behnken Design ◽  
Independent Variables ◽  
Order Polynomial ◽  
Tanning Process ◽  
Bovine Hide

Unhairing is one of the major steps of the leather tanning process which removes the hairs, epidermis and to some degree inter-fibrillary proteins. This process needs high amounts of chemicals that cause environmental difficulties. On the other hand, these chemicals are available in the wastewater of the Merox unit of Kermanshah Refinery. For reducing chemical consumption and decreasing environment pollution, we used the aforementioned wastewater for bovine unhairing. A unhairing liquor was prepared in order to unhair skins and different parameters such as the concentration of CaCO3 and Na2S, process time, and temperature were considered to evaluate their impact on the unhairing process and to obtain optimum condition. Response surface methodology based on Box-Behnken design was applied to model the unhairing efficiency in terms of four independent variables. A second-order polynomial equation was suggested to predict the response with high certainty. ANOVA revealed the validity and importance of that model by P-value of < 0.0001, large F-values, and, R2 of 98.8. The maximum unhairing efficiency of about 98.5 % was obtained under the optimum condition of 1.7 wt% Na2S, 5.8 wt% CaCO3, 114 min process time and 29.5 ºC.

scholarly journals PREPARATION OF ORAL CURCUMIN DELIVERY FROM 3D-NANO-CELLULOSE NETWORKS MATERIAL PRODUCED BY ACETOBACTER XYLINUM USING OPTIMIZATION TECHNIQUE

2020 ◽  
pp. 47-52
Author(s):  
THANH XUAN NGUYEN ◽  
HUONG LAN THI PHAM ◽  
THUONG THI NGO ◽  
PHONG XUAN ONG
Keyword(s):  
Oral Delivery ◽  
Positive Impact ◽  
Optimization Technique ◽  
Box Behnken Design ◽  
Independent Variables ◽  
3D Network ◽  
Nano Cellulose ◽  
Optimized Conditions ◽  
The Impact

Objective: To prepare oral curcumin delivery and optimize curcumin loading of 3D-nano-cellulose networks material (3DCM) by looking into the impact of process variables on the response utilizing response surface methodology (RSM) and Box-Behnken design. Methods: Optimization of curcumin loading of 3DCM was conducted using RSM and Box-Behnken model. Impact of four independent variables, including, the concentration of curcumin (X1), temperature (X2), shaking speed (X3), and time of loading (X4), was studied on one dependent response, that is, an amount of loaded curcumin (Y). Characterization of optimized 3DCM including curcumin was examined by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) analysis . Results: R2 value for Y was approximately 0.94. X1 possessed the biggest positive impact compared to X2, X3 and X4. Optimized conditions for curcumin loading of 3DCM were X1 at 3 mg/ml, X2 at 40 °C, X3 at 120 rpm and X4 at 120 min. SEM photograph of 3DCM surfaces were found including fibers creating a 3D network structure. FTIR spectra studies depicted that there was no interaction between curcumin and 3DCM. Conclusion: The data obtained in this study thus suggest that curcumin loaded 3DCM was successfully fabricated to give a potential oral delivery system of curcumin.

scholarly journals Fluoride removal efficiencies of Al-EC and Fe-EC reactors: process optimization using Box–Behnken design of the surface response methodology

2020 ◽  
Vol 10 (9) ◽  
Author(s):  
Magori J. Nyangi ◽  
Yonas Chebude ◽  
Kessy F. Kilulya
Keyword(s):  
Treatment Time ◽  
Fluoride Concentration ◽  
Fluoride Removal ◽  
Reactor Model ◽  
Optimal Operating ◽  
Surface Response Methodology ◽  
Box Behnken Design ◽  
Surface Response ◽  
Independent Variables ◽  
Initial Ph

Abstract In this study, surface response methodology was employed to investigate the effect of different interacting factors on the removal of fluoride from synthetic water using aluminum electrocoagulation (Al-EC) and iron electrocoagulation (Fe-EC) in different reactors. Box–Behnken design of a Design Expert version 11 was used for the optimization and evaluation of the process independent variables: applied electric density, initial pH, initial fluoride concentration and treatment time on the efficiency of fluoride removal as a response. Results showed that the effect of current density and initial fluoride concentration was significant model terms for fluoride reduction in Fe-EC and Al-EC reactors, respectively. The Al-EC reactor model presented the R2 value of 79.2% while Fe-EC presented R2 value of 75.8%, showing that both models can predict the response well. The reduction by 94% (initial concentration of 16 mgF/L) was established at optimal operating parameters of 18.5 mAcm−2, pH 6.80 in 50 min using Al-EC. On the other hand, 16 mgF/L was reduced by 92% to 1.28 mgF/L in Fe-EC reactor at optimal condition of 6.5 mAcm−2, pH 6.50 in 50 min. Experimental results correlated well to the model predicted results that were 95 and 94% for Al-EC and Fe-EC, respectively. Both reactors manage to reduce fluoride to a level recommended by WHO (≤ 1.5 mg/L) for drinking purpose.

Optimal Decolorization Efficiency of Reactive Red 3 by Fe-RH-MCM-41 Catalytic Wet Oxidation Coupled with Box-Behnken Design

2013 ◽  
Vol 545 ◽  
pp. 109-114 ◽  
Author(s):  
Kitirote Wantala ◽  
Pongsert Sriprom ◽  
Nusavadee Pojananukij ◽  
Arthit Neramittagapong ◽  
Sutasinee Neramittagapong ◽  
...  
Keyword(s):  
Surface Area ◽  
Hexagonal Lattice ◽  
Hexagonal Structure ◽  
Bet Surface Area ◽  
Molar Ratio ◽  
Wet Oxidation ◽  
Catalytic Wet Oxidation ◽  
Box Behnken Design ◽  
Independent Variables ◽  
Mcm 41

The reactive red 3 was degraded by catalytic wet oxidation process over Fe-RH-MCM-41 prepared by Direct Hydrothermal Technique (DHT) at Si/Fe molar ratio of 10 using silica from rice husk. The extended reaction conditions were studied as a function of reaction temperatures, initial H2O2 concentrations and initial pH of solutions designed by Box-Behnken design (BBD) based on Response Surface Methodology (RSM) to achieve the optimal condition and interaction of independent variables. The characterizations of catalyst were studied by XRD, BET surface area and TEM to explain the morphology of surface and to confirm the hexagonal structure. The results showed the 2theta peak can be indexed to hexagonal lattice that also confirmed by TEM result and surface area about 650 m2/g. All of independent variables showed significant on the degradation of reactive red 3 except for initial H2O2 concentration.

scholarly journals Optimization of Culture Medium for Lactobacillus bulgaricus using Box-Behnken Design

2017 ◽  
Vol 21 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Bowen Zhang ◽  
Guowei Shu ◽  
Chunju Bao ◽  
Jili Cao ◽  
Yuan Tan
Keyword(s):  
Culture Medium ◽  
Casein Hydrolysate ◽  
Commercial Production ◽  
Lactobacillus Bulgaricus ◽  
Dairy Production ◽  
Box Behnken Design ◽  
Medium Components ◽  
Viable Bacteria ◽  
Optimized Conditions ◽  
And Storage

AbstractLactobacillus bulgaricusis a common yogurt starter in dairy production. But the viable counts of the bacteria in the productions are relatively low during free-drying and storage which is not good for its commercial production. In order to obtain a medium with high activity and high density for bacterial cultured, the experiments and regression analysis were conducted by Box-Behnken design in this study, and a model was established to predict the influence of glucose (9-11 g·L−1), casein hydrolysate (15-17 g·L−1) and glutamate (6.5-7.5 mg·L−1) on viable counts ofL. bulgaricusand. The results showed that the glucose, 9.5 g·L−1; casein hydrolysate, 15.5 g·L−1; glutamate, 7.0mg·L−1, the number of viable bacteria ofL. bulgaricuscould reach (2.95±0.07) ×109, which was very similar to the predicted value of the model of 3.00×109cfu·mL−1, indicating that the optimized conditions and models used were feasible and effective. The optimized medium components can improve the viable counts of bacteria which are useful from its application in industrial production.

scholarly journals Current Advances in Microbial Production of Acetoin and 2,3-Butanediol by Bacillus spp.

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 307
Author(s):  
Kaloyan Petrov ◽  
Penka Petrova
Keyword(s):  
State Of The Art ◽  
Future Research ◽  
Huge Number ◽  
Genus Bacillus ◽  
Waste Glycerol ◽  
Synthetic Rubber ◽  
Lower Productivity ◽  
Printing Inks ◽  
Bacillus Spp ◽  
Growing Conditions

The growing need for industrial production of bio-based acetoin and 2,3-butanediol (2,3-BD) is due to both environmental concerns, and their widespread use in the food, pharmaceutical, and chemical industries. Acetoin is a common spice added to many foods, but also a valuable reagent in many chemical syntheses. Similarly, 2,3-BD is an indispensable chemical on the platform in the production of synthetic rubber, printing inks, perfumes, antifreeze, and fuel additives. This state-of-the-art review focuses on representatives of the genus Bacillus as prospective producers of acetoin and 2,3-BD. They have the following important advantages: non-pathogenic nature, unpretentiousness to growing conditions, and the ability to utilize a huge number of substrates (glucose, sucrose, starch, cellulose, and inulin hydrolysates), sugars from the composition of lignocellulose (cellobiose, mannose, galactose, xylose, and arabinose), as well as waste glycerol. In addition, these strains can be improved by genetic engineering, and are amenable to process optimization. Bacillus spp. are among the best acetoin producers. They also synthesize 2,3-BD in titer and yield comparable to those of the pathogenic producers. However, Bacillus spp. show relatively lower productivity, which can be increased in the course of challenging future research.

scholarly journals THE ROLE OF THE FOREST COMPLEX IN THE SOCIO-ECONOMIC DEVELOPMENT OF THE REGION (ON THE EXAMPLE OF THE REPUBLIC OF MARIY EL)

2018 ◽  
Vol 8 (1) ◽  
pp. 208-219
Author(s):  
Татьяна Мочаева ◽  
Tat'yana Mochaeva ◽  
Алексей Шургин ◽  
Aleksey Shurgin ◽  
Андрей Туев ◽  
...  
Keyword(s):  
Raw Materials ◽  
Paper Mill ◽  
Forest Resources ◽  
Added Value ◽  
Forest Sector ◽  
Pulp And Paper Mill ◽  
Forest Protection ◽  
Protection Measures ◽  
The Republic ◽  
Growing Conditions

The strategic goal of the development of the Russian forest complex is to significantly increase its contribution to the national economy by creating an economically sustainable, private and globally competitive group of industries. For this, the products of forest sector of Russia should enjoy equal demand both on domestic and foreign markets. The basic branch of forest complex is forestry, which provides protection, reproduction of forests, regulation of forest man-agement and control over the use of forest resources. The Republic of Mariy El is not distinguished by the presence of a large number of industrially significant natural resources. The main natural wealth of the region is forest, which covers more than 50% of its territory. The forest-growing conditions of the Republic of Mariy El in most of its territory are favorable for the growth of valuable coniferous species. Extensive exploitation of the republic's forests is a difficult period of the beginning and the middle of the 20th century, traditional ways of managing in the forest, aimed primarily at selecting the most valuable timber; insufficient financing of reforestation, forest protection measures; as well as cur-rent market situation of timber currently lead to negative changes in species and commodity structure of the country's forest resources. The locomotive of the growth of the forest complex of the Republic of Mariy El is JSC Mari Pulp and Paper Mill, which largely covers its need for wood raw materials by importing it from outside the region. Enterprises-tenants of forest areas carry out the harvesting of timber and subsequent sale of products with low added value, which reduces the contribution of forestry industries to the economy of the region. Forest recreation should be a strategic di-rection for the development of forest management in the Republic of Mariy El.

scholarly journals Penerapan Algoritme Genetik Untuk Seleksi Peubah Regresi Logistik

2020 ◽  
Vol 9 (1) ◽  
pp. 51-58
Author(s):  
Dian Ayuningtyas ◽  
Bagus Sartono ◽  
Farit Mochamad Afendi
Keyword(s):  
Genetic Algorithm ◽  
Variable Selection ◽  
Optimization Method ◽  
Response Variable ◽  
Absolute Value ◽  
Independent Variables ◽  
Interaction Factors ◽  
Independent Variable ◽  
Positive Effect ◽  
Excessive Number

In a study, interaction factors are the potential to have important effects on the response variable. But research involving interaction factors often encounters two problems, namely the excessive number of variables and the difficulty of implementing the heredity principle. The alternative solution is to do variable selection using a metaheuristic optimization method, In this study, the logistic regression variable selection was done using a genetic algorithm. The genetic algorithm is modified so that every independent variable has a different probability to be included in the model. That probability is based on the absolute value of the correlation of the independent variable with the response variable. These modifications have a positive effect on the results of variable selection. To choose significant independent variables, 30 repetitions of the genetic algorithm can be performed using the objective function AIC. Of the 30 repetitions, if a variable appears in all formed models, then the variable is an independent variable that has a significant effect on the response variable. The application of this method to Myopia data can show significant variables well.

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