scholarly journals Effect of wound healing in gels containing tinctures of Alnus glutinosa (L) leaves

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Volha Mushkina
Keyword(s):  
Wound Healing ◽  
Alnus Glutinosa ◽  
Skin Wound ◽  
Complete Healing ◽  
New Drugs ◽  
Laboratory Animals ◽  
Control Group ◽  
Wound Area ◽  
Reduction Percentage ◽  
Wound Healing Activity

Abstract Background Alnus glutinosa (L) is a widespread medicinal plant on the territory of the Republic of Belarus. Alnus glutinosa (L) leaves tincture has an anti-inflammatory effect on the diseases of the throat, intestines, in rheumatism and gout. Water extracts from collective fruit, leaves and bark are used for gargling in stomatitis and gum bleeding. The purpose of the research was to establish wound healing activity of gels based on alcohol extracts from Alnus glutinosa (L) leaves on the model of a surface full flap skin wound on laboratory animals. Methods Regenerating action of new drugs (gels) based on tinctures of Alnus glutinosa (L) leaves was studied on a model of planar full-layer skin wound in laboratory animals. Wounds were treated daily with gels applied to complete healing. The wound area was measured planometrically to assess the effectiveness of drugs and then the percentage of area reduction was calculated. Regenerative action of the gels developed was compared to widely used in medical practice 4% Dexpanthenol Gel and Reparef-2 Ointment. 15 g of Reparef-2 Ointment contains 1200 mg of bien and 75 mg of dioxidine. Results Wound healing activity of gels containing tinctures of Alnus glutinosa (L) leaves based on 30% and 60% alcohol as to the rate of healing and the area of wounds epithelialization has been proved. Wound area treated with the gel based on Alnus glutinosa (L) extract containing 60% alcohol on day 13 of the experiment decreased by 95.59% ± 0.9% (p ≤ 0.05); based on the tincture containing 30% alcohol - by 92.93% ± 1.11%. Medicinal preparations with proved wound healing activity were compared: Reparef-2 Ointment (reduction percentage of wound area on day 13 of the experiment made 92.24% ± 2.21%) and Dexpanthenol Gel (reduction percentage of wound area on day 13 of the experiment made 83.09% ± 3.41%). Compared with the control group the gel based on 60% tincture of Alnus glutinosa (L) leaves showed a decrease in the epithelialization period by 29.5%; based on 30% tincture - by 24.6%; Reparef-2 Ointment - by 23.6% and Dexpanthenol Gel - by 13.8%. Conclusions The results of this study showed that the developed gels based on Alnus glutinosa (L) have a regenerating action and can be used to treat wound processes taking into account previously established antimicrobial activity.

scholarly journals Wound Healing Study of Panchavalkal Ointment in Wistar Albino Rats

2021 ◽  
pp. 110-116
Author(s):  
Vaikhari Dhurve ◽  
Pravin Jawanjal ◽  
Mukesh Naria ◽  
Tukaram Dudhamal ◽  
Minal Kalambe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Wound Area ◽  
Mean Values ◽  
Wistar Strain ◽  
The Mean ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

Introduction: Panchavalkal is a well-known Ayurvedic poly-herbal formulation that has been reported to be used against inflammation, to clean ulcer, wound. Aims and Objectives: To investigate the wound healing activity of Panchavalkal ointment. Materials and Methods: Wistar strain albino rats of either sex weighing 200±20 g were used for the experiments divided in four groups each consisted of six rats.  Statistical Analysis: One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variables among the groups followed by Dunnett’s multiple ‘t’ test Observations & Results: Sesame oil and Panchvalkal ointment showed almost similar wound healing effects in comparison to control group. Discussion: Panchavalkal ointment showed statistically highly significant percentage of contraction of excision wound area compared to the normal control. Epithelization period was significantly decreased in oil and Panchavalkal ointment treated group. Conclusion: Panchavalkal ointment decreased the pain, tenderness, redness and swelling that helps to control infection and enhanced the rate of wound healing in albino rats.

scholarly journals The Combined Effect of Photobiomodulation and Curcumin on Acute Skin Wound Healing in Rats

2021 ◽  
Vol 12 ◽  
pp. e9-e9
Author(s):  
Abdollah Amini ◽  
Hasan Soleimani ◽  
Fatemehalsadat Rezaei ◽  
Seyed Kamran Ghoreishi ◽  
Sufan Chien ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Skin Wound ◽  
Control Group ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Group 4 ◽  
Patient Morbidity ◽  
Group 3 ◽  
Combined Impact

Introduction: Abnormal wound repair is a cause for considerable expense, as well as patient morbidity and mortality. Here, we investigated the combined impact of photobiomodulation (PBM) and curcumin on a rat experimental model of an acute skin wound. Methods: A round full-thickness wound was created on the back of each rat. We divided the rats into the following four groups. Group one was the control group. Group two received pulse wave (PW) PBM at a dose of 890 nm, 80 Hz, and 0.2 J/cm2 . Group 3 received 40 mg/kg curcumin by gastric gavage and group 4 were treated with PWPBM + curcumin. We measured the wound area on days 4, 7, and 15, and performed microbiological and tensiometric examinations. Results: There was markedly improved wound contraction in the curcumin (7.5 ± 0.57; P=0.000), PBM (8.5 ± 1.2; P=0.000), and PBM + curcumin (14.5 ± 4.3; P=0.002) groups relative to the control group (25 ± 6). PBM (100 ± 7.3; P=0.005), and PBM + curcumin (98 ± 6; P=0.005) groups meaningfully improved tensile strength relative to the control group (61 ± 8.2). On day 15, the PBM (10 ± 5; P=0.000), curcumin (14 ± 4.5, P=0.000), and PBM + curcumin (27.3 ± 8.3; P=0.000) groups meaningfully decreased microbial flora relative to the control group (95 ± 6). Conclusion: We concluded that the PBM and PBM + curcumin groups meaningfully accelerated wound healing of the acute skin wound in the rats. The results of the PBM group were statistically more effective than the curcumin alone and PBM + curcumin-treated groups.

scholarly journals Formulation and Evaluation of Wound Healing Effect of Aqueous Herbal Cream Containing Various Extracts of Passiflora foetida Linn., Leaves

2019 ◽  
pp. 490-499
Author(s):  
Akila E ◽  
C. Geetha Priya
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Soft Tissues ◽  
Folk Medicine ◽  
New Drugs ◽  
Herbal Remedies ◽  
Control Group ◽  
Wound Model ◽  
Number Of Patients ◽  
Passiflora Foetida

Wounds are a major cause of concern for the patient and clinician alike; chronic Wounds affect a large number of patients and seriously reduce their quality of life. Wound healing is the process of repair that follows injury to the skin and other soft tissues. A cream is a preparation of a medication for topical use that contains a water base. Essentially, it is a preparation of oil in water. Herbal remedies used in folk medicine provide an interesting and still largely unexplored source for the creation and development of potentially new drugs, which might help to overcome the growing problem of resistance and also the toxicity of the currently available commercial antibiotics. In this study we have formulated an Aqueous herbal cream satisfying almost all pharmaceutical parameters which shows better wound healing activity. The wound healing of effect of Aqueous Herbal cream of various extracts of leaves of Passiflora foetida L. was evaluated by incision and excision wound model. The experimental results and histopathological studies showed that Aqueous Herbal Cream of Passiflora foetida L. leaves exhibits significant wound healing property as compared to control group of animals.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

Application of paste-type acellular dermal matrix in hard-to-heal wounds

2021 ◽  
Vol 30 (5) ◽  
pp. 414-418
Author(s):  
Sang Wha Kim ◽  
Hyung Sup Shim ◽  
Jihye Lee ◽  
Youn Hwan Kim
Keyword(s):  
Wound Healing ◽  
Adverse Events ◽  
Acellular Dermal Matrix ◽  
Complete Healing ◽  
Dermal Matrix ◽  
Wound Area ◽  
Number Of Patients ◽  
Complete Wound Healing ◽  
Zero Baseline ◽  
Acellular Dermal

Objective: The extracellular matrix (ECM) is one of the most important elements in wound healing. Absence or dysfunction of the ECM may impair wound healing. The application of acellular dermal matrix (ADM) as a substitute for ECM has been suggested. This study investigated the clinical application and wound healing effects of a paste-type ADM in patients presenting with hard-to-heal wounds due to various causes. Method: Patients with a hard-to-heal wound for >1 month, from September 2017 to February 2019, were included in this study. After debridement, the paste-type ADM was applied, at zero (baseline), two and four weeks. After application of the paste-type ADM, a conventional dressing was applied using polyurethane foam. Wound size, the formation of granulation tissue, re-epithelialisation, complete healing and adverse events were recorded at zero (baseline), one, two, four, eight and 12 weeks after the initial treatment. Results: A total of 18 patients took part (eight male, 10 female, mean age of 56±16.16 years). The mean wound area decreased from 17.42±10.04cm2 to 12.73±7.60cm2 by week one (p<0.05), to 10.16±7.00 by week two (p<0.0005), to 5.56±5.25 by week four (p<0.0001), to 2.77±5.15 by week eight (p<0.0001) and to 2.07±4.78 by week 12 (p<0.0001). The number of patients with >75% re-epithelialisation increased from two (11.1%) at two weeks to five (27.8%) at four weeks, to 11 (61.1%) at eight weeks and to 13 (72.2%) at 12 weeks. The number of patients showing complete wound healing was two (11.1%) at four weeks, nine (50.0%) at eight weeks and 12 (66.7%) at 12 weeks. No adverse events were reported during treatment. Conclusion: The paste-type ADM used in this study is a viable option for facilitating wound healing; it can shorten hospitalisation, and promote a faster recovery and return to normal life activities.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

scholarly journals Phytochemical Composition, Antioxidant Activity, and the Effect of the Aqueous Extract of Coffee (Coffea arabica L.) Bean Residual Press Cake on the Skin Wound Healing

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Regina Celis Lopes Affonso ◽  
Ana Paula Lorenzen Voytena ◽  
Simone Fanan ◽  
Heloísa Pitz ◽  
Daniela Sousa Coelho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Press Cake ◽  
Negative Control ◽  
Skin Wound ◽  
Coffee Bean ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Inflammatory Phase ◽  
Coffee Beans

The world coffee consumption has been growing for its appreciated taste and its beneficial effects on health. The residual biomass of coffee, originated in the food industry after oil extraction from coffee beans, called coffee beans residual press cake, has attracted interest as a source of compounds with antioxidant activity. This study investigated the chemical composition of aqueous extracts of coffee beans residual press cake (AE), their antioxidant activity, and the effect of topical application on the skin wound healing, in animal model, of hydrogels containing the AE, chlorogenic acid (CGA), allantoin (positive control), and carbopol (negative control). The treatments’ performance was compared by measuring the reduction of the wound area, with superior result (p<0.05) for the green coffee AE (78.20%) with respect to roasted coffee AE (53.71%), allantoin (70.83%), and carbopol (23.56%). CGA hydrogels reduced significantly the wound area size on the inflammatory phase, which may be associated with the well known antioxidant and anti-inflammatory actions of that compound. The topic use of the coffee AE studied improved the skin wound healing and points to an interesting biotechnological application of the coffee bean residual press cake.

scholarly journals PENGARUH PENGGUNAAN ASI PADA PERAWATAN LUKA PERINIEM DENGAN LAMA WAKTU PENYEMBUHAN LUKA PERINIUM

2019 ◽  
Vol 7 (2) ◽  
pp. 67-73
Author(s):  
Annisa Ul Mutmainnah ◽  
Siti Noorbaya
Keyword(s):  
Wound Healing ◽  
Data Analysis ◽  
Random Sampling ◽  
Wound Care ◽  
Healing Time ◽  
Natural Process ◽  
Control Group ◽  
P Value ◽  
Research Subjects ◽  
Wound Area

Childbirth is a natural process, but sometimes labor can also cause birth canaltrauma, especially in the aluminum region, this trauma can be injuries to the periniumarea, the cause can be intentional biases such as episiotomy or unintentional actions such as spontaneous tears in the process of removing the baby. Injury to the perineum if not treated properly can cause postpartum infection because the wound area will be a medium for developing germs. The purpose of this study was to analyze the effect of the use of breast milk in the treatment of periniem wounds with the duration of perinium wound healing at the Ramlah Parjib Clinic in Samarinda. The method used in this study was Posttest Only Control Group Desigen research subjects were postpartum motherswith a sample of 60 respondents taken by random sampling and grouped based on treatment using ASI. Data analysis using ANOVA test. The results showed that the use of ASI had a significant effect on the treatment of periniem wounds with a healing time seen from the P value of 0,000. Conclusion: Periniuem wound care techniques are factors that influence the duration of perinieum wound healing

scholarly journals Evaluation of Indonesian and two types of Japanese honey on the acceleration of cutaneous wound healing: an experimental study in mice

2019 ◽  
Vol 2 (2) ◽  
pp. 80-88
Author(s):  
Haryanto Haryanto ◽  
Kanae Mukai ◽  
Nakajima Yukari ◽  
Mayumi Okuwa ◽  
Cau Kim Jiu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Wound Area ◽  
Hydrocolloid Dressing ◽  
Thickness Skin ◽  
Microscopic Evaluation ◽  
Acacia Honey ◽  
Macroscopic Findings

The purpose of this study is to evaluate and clarify the effect of Indonesian honey, two types of Japanese honey and hydrocolloid dressing on wound healing process. Four groups of male mice were treated to produce two circular full-thickness skin wounds on the dorsum. They were then randomly allocated to receive daily Indonesian honey, Japanese honey (Buckwheat and Acacia honey) or hydrocolloid dressing as a control for treatment application. Macroscopic findings were observed from day 0 to 14 after wounding. Microscopic evaluation was assessed using qualitative analysis. The ratios of wound areas for honey groups on day 3 and 7 were smaller than those of the control group. Wound areas of honey groups gradually decreased to almost the same wound area as the control group on day 14, while the wound area of the control group peaked on day 5 and rapidly decreased until day 14. Microscopic finding that Indonesian honey was different with Japanese honey especially Buckwheat honey.

scholarly journals The effect of Curcuminvs. Curcumin combined Antibiotic Ointmentson the Healing of skin wound

2021 ◽  
Vol 14 (3) ◽  
pp. 1587-1593
Author(s):  
Emad K. Abbas ◽  
Hussein H. Echrish ◽  
Sabaa A. Mohammed
Keyword(s):  
Wound Healing ◽  
Normal Saline ◽  
Biological Effects ◽  
Skin Wound ◽  
Control Group ◽  
P Value ◽  
Average Weight ◽  
Wide Range ◽  
Significant Difference ◽  
Animal House

Background:Turmeric is typically used as a spicy food preservative and colorant. It has been proved that curcumin has a wide range of biological effects including anti-inflammatory, anti-viral, anti-fungal, and curcumin activity that can improve antibiotic activity on the wounds. Objectives: To evaluate the effects of Curcumin with and without antibiotics on skin wound treatment. Materials and Methods: The protocol was approved by the animal house in medical college / Basra university. This study used nine male rabbits aged about 6 months and an average weight of (1.083 g). Each group consists of 3 rabbits: control group (normal saline) A, topical curcumin in group B, topical curcumin, and tetracycline ointment in group C. Regular treatments were given to rabbits in therapeutic groups. Result: The lowest Mean ± SDof swelling of suturing area was noted in both groups that treated by curcumin alone (9.07 ± 0.97 vs 15±1 mm, p value = 0.002) and that treated with curcumine and antibiotic (9.1±0.9vs 15±1 mm, p value = 0.002) versus the control group ( that treated by normal saline) and the lowest Mean ± SD of elevation of suture line was noted in both group that treated by curcumin alone (2.63 ± 0.06 vs 4.07±0.21 mm, p value >0.001) and that treated with curcumin and antibiotic (2.7 ± 0.2 vs 4.07±0.21 mm, p value =0.001) versus control group. There is no significant statistical difference between the Mean ± SD neither of swelling of suture area nor of elevation of suture lines of groups that treated by curcumin alone and group that treated with curcumin and antibiotic [(9.07 ± 0.97 vs 9.1 ±0.9, p value=0.97),(2.63±0.06 vs 2.7 ± 0.2, p value=0.61) respectively]. The histopathological evaluation is consistent with morphological changes as at day 3 of wound healing in both groups that treated by curcumine with and without antibiotic, there is formation a thin layer of keratin and absence of features that indicate delay wound healing such as hemorrhage, inflammatory cell infiltrate of (Neutrophils, macrophages and lymphocytes) and debris, which are detected in control group. Furthermore, at day 7 of control group, there is decrease of inflammation, presence of gap between the two edgesof the wound but no keratin formation. No clear histopathological difference in wound healing between tested groups that treated by curcumin with and without antibiotic. Conclusion: There issignificant clinical and histological evidences that the curcumin not only prevent delay of wound healing but it is also enhanced wound healing. No significant difference in using curcumin alone or combine it with local antibiotic.

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