Screening of antidiabetic and antioxidant potential along with phytochemicals of Annona genus: a review

Abstract Background Annona species can be found in the subtropical and tropical parts of the world. Because of their medicinal capabilities and highly exotic edible fruits, they are one of the most important members of the Annonaceae family. Isoquinolines, pyrimidine-β-carboline alkaloids, lectins, acetogenins, and volatile oils are among the active metabolites found in this genus, all of which have been shown to have anti-diabetic and antioxidant activities. Main body The fundamental objective of this review was to summarize the antidiabetic and antioxidant activity based on reported secondary data from different plants of the genus Annona. These species include Annona cherimola, Annona squamosa, Annona macroprophyllata, Annona muricate, Annona reticulata, Annona carcans, Annona coriacea, Annona cornifolia, and Annona senegalensis. The Annona species investigated had significant antihyperglycemic and antioxidant properties. Conclusion The available evidence, both in vitro and in vivo, confirms the ability of Annona species to treat diabetes in addition to producing oxidative damage.

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Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem098 ◽

2009 ◽

Vol 6 (2) ◽

pp. 227-231 ◽

Cited By ~ 28

Author(s):

S. A. Adesegun ◽

A. Fajana ◽

C. I. Orabueze ◽

H. A. B. Coker

Keyword(s):

Ascorbic Acid ◽

Leaf Extract ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

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Phytochemical analysis and nephroprotective effect of cactus (Opuntia ficus-indica) cladodes on sodium dichromate-induced kidney injury in rats

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2018-0184 ◽

2019 ◽

Vol 44 (3) ◽

pp. 239-247

Author(s):

Mbarka Hfaiedh ◽

Dalel Brahmi ◽

Mohamed Nizar Zourgui ◽

Lazhar Zourgui

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Wistar Rat ◽

Phytochemical Analysis ◽

Sodium Dichromate ◽

Opuntia Ficus Indica

Environmental and occupational exposure to chromium compounds, especially hexavalent chromium, is widely recognized as potentially nephrotoxic in humans and animals. The present study aimed to assess the efficacy of cactus (Opuntia ficus-indica) against sodium dichromate-induced nephrotoxicity, oxidative stress, and genotoxicity. Cactus cladodes extract (CCE) was phytochemically studied and tested in vitro for its potential antioxidant activities. Additionally, the preventive effect of CCE against sodium dichromate-induced renal dysfunction in a Wistar rat model (24 rats) was evaluated. For this purpose, CCE at a dose of 100 mg/kg was orally administered, followed by 10 mg/kg sodium dichromate (intraperitoneal injection). After 40 days of treatment, the rats were sacrificed, and the kidneys were excised for histological, lipid peroxidation, and antioxidant enzyme analyses. The phenol, flavonoid, tannin, ascorbic acid, and carotenoid contents of CCE were considered to be important. Our analyses showed that 1 mL of CCE was equivalent to 982.5 ± 1.79 μg of gallic acid, 294.37 ± 0.84 μg of rutin, 234.78 ± 0.24 μg of catechin, 204.34 ± 1.53 μg of ascorbic acid, and 3.14 ± 0.51 μg of β-carotene. In vivo, pretreatment with CCE was found to provide significant protection against sodium dichromate-induced nephrotoxicity by inhibiting lipid peroxidation, preserving normal antioxidant activities, and protecting renal tissues from lesions and DNA damage. The nephroprotective potential of CCE against sodium dichromate toxicity might be due to its antioxidant properties.

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Antinociceptive and Antioxidant Activities of Phytol In Vivo and In Vitro Models

Neuroscience Journal ◽

10.1155/2013/949452 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 79

Author(s):

Camila Carolina de Menezes Patrício Santos ◽

Mirian Stiebbe Salvadori ◽

Vanine Gomes Mota ◽

Luciana Muratori Costa ◽

Antonia Amanda Cardoso de Almeida ◽

...

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Thiobarbituric Acid ◽

In Vitro Models ◽

Control Group ◽

Second Phase ◽

Hot Plate Test ◽

Antinociceptive Effects

The objective of the present study was to evaluate the antinociceptive effects of phytol using chemical and thermal models of nociception in mice and to assess its antioxidant effects in vitro. Phytol was administered intraperitoneally (i.p.) to mice at doses of 25, 50, 100, and 200 mg/kg. In the acetic acid-induced writhing test, phytol significantly reduced the number of contortions compared to the control group (P<0.001). In the formalin test, phytol reduced significantly the amount of time spent in paw licking in both phases (the neurogenic and inflammatory phases), this effect being more pronounced in the second phase (P<0.001). Phytol also provoked a significant increase in latency in the hot plate test. These antinociceptive effects did not impaire the motor performance, as shown in the rotarod test. Phytol demonstrated a strong antioxidant effect in vitro in its capacity to remove hydroxyl radicals and nitric oxide as well as to prevent the formation of thiobarbituric acid reactive substances (TBARS). Taken as a whole, these results show the pronounced antinociceptive effects of phytol in the nociception models used, both through its central and peripheral actions, but also its antioxidant properties demonstrated in the in vitro methods used.

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Synthesis, Characterization of Pyrano-[2,3-c]-Pyrazoles Derivatives and Determination of Their Antioxidant Activities

Iranian Jornal of Toxicology ◽

10.32598/ijt.15.3.798.1 ◽

2021 ◽

Vol 15 (3) ◽

pp. 175-194

Author(s):

Boutaina Addoum ◽

◽

Bouchra El khalfi ◽

Mohamed Idiken ◽

Souraya Sakoui ◽

...

Keyword(s):

Oxidative Stress ◽

High Performance ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Model Organism ◽

Biologically Active ◽

Strong Activity ◽

Nitrative Stress

Background: Antioxidants are developed to assist the immune system and overcome oxidative stress, the aggression of cellular constituents due to imbalance between reactive oxygen species and the inner antioxidant system. The main objective of this study was to search for new and potent antioxidants to protect humans against diseases associated with oxidative stress. Methods: In this study, three pyrano-[2,3-c]-pyrazole derivatives were synthesized via Multicomponent Reaction (MCR) approach and were characterized, using a melting point, High-Performance Liquid Chromatography (HPLC), and spectroscopic analyses (IR; 1H-NMR; 13C-NMR). All of the generated compounds were screened for their antioxidant properties in vivo, using ciliate “Tetrahymena” as a model organism exposed to oxidative and nitrative stress. They were then studied in vitro by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Results: The results demonstrated that the three compounds (5a, b, c) are biologically active and possess potent antioxidant activities, especially the 5a and 5b derivatives. On the other hand, the in vitro bioassays revealed that the 5a derivative possessed a significant antioxidant activity much greater than ascorbic acid. Accordingly, the in silico data are consistent with the experimental data. Conclusion: These findings confirmed the potent antioxidant property of the synthesized compounds, providing us with new inspiration and challenges to design a library of pharmaceutical compounds with strong activity and low toxicity in the future.

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Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats

10.20944/preprints201803.0148.v1 ◽

2018 ◽

Author(s):

Songul Cetik Yildiz ◽

Cumali Keskin ◽

Adnan Ayhanci

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Stress Index ◽

Total Phenolic ◽

Rat Tissues ◽

Protective Effects ◽

Methanol Extracts

The aim of this study was to investigate in-vitro antioxidant properties and in-vivo protective effects of different concentrations of Hypericum triquetrifolium Turra. (HT) seed methanol extracts against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats exposed to overdose of cyclophosphamide (CP). HT seed methanol extracts were tested in view of its in-vitro antioxidant activities as total phenolic contents and DPPH free radical-scavenging activity. To investigate in-vivo protective effects of HT seed methanol extracts on rat tissues; tested animals were divided into nine groups. Three groups only were treated with HT extracts (25, 50 and 100 mg/kg HT) for 6 days. Three groups were pre-treated with the extract of HT (25, 50 and 100 mg/kg HT) for 6 days and on the last day they were injected with single dose of CP (150-mg/kg body weight). Two groups were used as control groups and one group was only treated with CP (150 mg/kg) on the 6th day. The toxic effects of CP and protective effects of HT extracts on the nucleated cells which were produced by bone marrow and serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined histopathologically. Our results show that HT seed methanol extract has high total phenolic content and antioxidant activity. Over dose CP administration caused hepatotoxicity, myelotoxicity and hematotoxicity on rat. Whereas, 25, 50 and 100 mg/kg HT plus CP administered groups showed significant protective effects on nucleated cells. And 25, 50, 100 mg/kg HT plus CP treated groups showed an important decrease on serum ALT, ALP, LDH and OSI levels when compared with CP treated group. Our results showed that the administration of different HT doses with high doses of CP significantly reduced hepatotoxicity, myelotoxicity and hematoxicity on rats.

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Comparative Study of Piper sylvaticum Roxb. Leaves and Stems for Anxiolytic and Antioxidant Properties Through In Vivo, In Vitro, and In Silico Approaches

Biomedicines ◽

10.3390/biomedicines8040068 ◽

2020 ◽

Vol 8 (4) ◽

pp. 68 ◽

Cited By ~ 5

Author(s):

Md. Adnan ◽

Md. Nazim Uddin Chy ◽

A.T.M. Mostafa Kamal ◽

Md Obyedul Kalam Azad ◽

Kazi Asfak Ahmed Chowdhury ◽

...

Keyword(s):

Molecular Docking ◽

In Silico ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Reducing Power ◽

Docking Study ◽

Molecular Docking Study ◽

Dose Dependent

Piper sylvaticum Roxb. is traditionally used by the indigenous people of tropical and subtropical countries like Bangladesh, India, and China for relieving the common cold or a variety of chronic diseases, such as asthma, chronic coughing, piles, rheumatic pain, headaches, wounds, tuberculosis, indigestion, and dyspepsia. This study tested anxiolytic and antioxidant activities by in vivo, in vitro, and in silico experiments for the metabolites extracted (methanol) from the leaves and stems of P. sylvaticum (MEPSL and MEPSS). During the anxiolytic evaluation analyzed by elevated plus maze and hole board tests, MEPSL and MEPSS (200 and 400 mg/kg, body weight) exhibited a significant and dose-dependent reduction of anxiety-like behavior in mice. Similarly, mice treated with MEPSL and MEPSS demonstrated dose-dependent increases in locomotion and CNS simulative effects in open field test. In addition, both extracts (MEPSL and MEPSS) also showed moderate antioxidant activities in DPPH scavenging and ferric reducing power assays compared to the standard, ascorbic acid. In parallel, previously isolated bioactive compounds from this plant were documented and subjected to a molecular docking study to correlate them with the pharmacological outcomes. The selected four major phytocompounds displayed favorable binding affinities to potassium channel and xanthine oxidoreductase enzyme targets in molecular docking experiments. Overall, P. sylvaticum is bioactive, as is evident through experimental and computational analysis. Further experiments are necessary to evaluate purified novel compounds for the clinical evaluation.

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Geographic Variation in the Chemical Composition and Antioxidant Properties of Phenolic Compounds from Cyclocarya paliurus (Batal) Iljinskaja Leaves

Molecules ◽

10.3390/molecules23102440 ◽

2018 ◽

Vol 23 (10) ◽

pp. 2440 ◽

Cited By ~ 7

Author(s):

Yang Liu ◽

Pei Chen ◽

Mingming Zhou ◽

Tongli Wang ◽

Shengzuo Fang ◽

...

Keyword(s):

Phenolic Compounds ◽

High Performance ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Geographic Origin ◽

Cyclocarya Paliurus ◽

Sampling Locations ◽

Kaempferol Glycosides

Cyclocarya paliurus has been widely used as an ingredient in functional foods in China. However, the antioxidant properties of phenolic compounds and the effect of the plant origin remain unclear. The present study evaluated the geographical variation of this plant in term of its phenolic composition and antioxidant activities based on leaf materials collected from five regions. high-performance liquid chromatography (HPLC) analysis showed that there are three major components, quercetin-3-O-glucuronide, kaempferol-3-O-glucuronide, and kaempferol-3-O-rhamnoside, and their contents varied significantly among sampling locations. The investigated phenolic compounds showed substantial antioxidant activities, both in vitro and in vivo, with the highest capacity observed from Wufeng and Jinzhongshan. Correlation analysis revealed that quercetin and kaempferol glycosides might be responsible for the antioxidant activities. Our results indicate the importance of geographic origin, with sunny hours and temperature as the main drivers affecting the accumulation of C. paliurus phenolics and their antioxidant properties.

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Antioxidant and Antisenescence Effects of Bergamot Juice

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/9395804 ◽

2018 ◽

Vol 2018 ◽

pp. 1-14 ◽

Cited By ~ 13

Author(s):

Eleonora Da Pozzo ◽

Marinella De Leo ◽

Immacolata Faraone ◽

Luigi Milella ◽

Chiara Cavallini ◽

...

Keyword(s):

High Performance ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Natural Antioxidant ◽

Antioxidant Compounds ◽

Cellular Models ◽

Health Promoting ◽

Phenolic And Flavonoid

Aging is one of the main risk factor for the onset of cardiovascular diseases; one of the possible explanations could be linked to the age-associated overproduction of free radicals. This increase of oxidative stress can be overcome with a high intake of food antioxidants. In this context, a number of studies have been addressed to assess the antiaging potential of natural antioxidant compounds. Recently, it has been shown that the juice of bergamot (Citrus bergamia Risso et Poiteau), a fruit mostly produced in the Ionian coastal areas of Southern Italy (Calabria), is a valuable source of health-promoting constituents with, among other, antioxidant properties. In order to investigate the potential antiaging effects of this Mediterranean natural antioxidant source, bergamot juices of three different cultivars (“fantastico,” “femminello,” and “castagnaro”) were herein characterized by the mean of high-performance liquid chromatography-photodiode array-electrospray ionization-tandem mass spectrometry. Then, juices were investigated for the evaluation of total polyphenolic and flavonoid contents, cell-free model antioxidant activities, and in vitro antiaging properties on two different cellular models of induced myocardial senescence. The best performing juice was also assessed in vivo. The phytochemical profiles confirmed that juices were rich in flavonoids, both flavone and flavanone glycosides. In addition, two limonoid glycosides were also identified in all cultivars. Each cultivar showed different phenolic and flavonoid contents. In tube results showed the juice robust antioxidant activities that correlate with their phenolic and flavonoid contents. Moreover, for the first time, the ability of juice to counteract the chemical-induced senescence was here demonstrated in both cellular models. Lastly, the in vivo data obtained from mouse hearts evidenced an increase in transcription of genes involved in antiaging and antioxidant responses. The overall results suggest that bergamot juice exerts antioxidant and antisenescence effects, making it useful for nutraceutical purposes.

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Potential In Vitro and In Vivo Antioxidant Activities from Piper Crocatum and Persea Americana Leaf Extracts

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1686 ◽

2019 ◽

Vol 12 (2) ◽

pp. 661-667

Author(s):

Fatmawaty Fatmawaty ◽

Ni G. M. Anggreni ◽

Naufal Fadhil ◽

Vivitri D. Prasasty

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Persea Americana ◽

Health Claims ◽

In Vitro Assays ◽

Moderate Activity ◽

Leaf Extracts ◽

Water Fraction

Piper crocatum and Persea americana Mill leaves are commonly used in traditional medicinal remedies, such as antidiabetes, antitumors and Alzheimer treatment. However, the extensive use of plant extracts over worldwide becomes significant concerns including its safety, efficacy, and quality. Therefore, the accurate scientific evaluation has become a precondition for acceptance of herbal health claims. The aim of this study was to determine the antioxidant activities of Piper crocatum Ruiz & Pav and Persea americana Mill leaf extracts. Isolation of antioxidant fractions were conducted using organic solvent extraction techniques. Antioxidant assays were conducted by using in vitro and in vivo methods involving DPPH and MDA methods. In this study, in vitro assays of Persea americana Mill and Piper crocatum Ruiz & Pav leaf extracts showed the best activity in water fraction. Moreover, in vivo assays of both plant leaf extracts showed the best dose at 8 mg. Persea americana Mill and Piper crocatum Ruiz & Pav leaf extracts have been successfully determined in antioxidant actions in vitro and in vivo. Persea americana Mill in water and ethanol solvents exhibit strong antioxidant properties. Meanwile, Piper crocatum Ruiz & Pav exhibit moderate activity in water and weak antioxidant activity in ethanol. Both plant leaves showed that 8 mg dose was better than the dose of 4 mg and 16 mg in vivo.

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In Vivo Anti-Inflammatory and In Vitro Antioxidant Activities of Moroccan Medicinal Plants

Natural Product Communications ◽

10.1177/1934578x1100601007 ◽

2011 ◽

Vol 6 (10) ◽

pp. 1934578X1100601

Author(s):

Mina Moussaid ◽

Abd Elaziz Elamrani ◽

Nourdinne Bourhim ◽

Mohamed Benaissa

Keyword(s):

Antioxidant Activities ◽

Inflammatory Diseases ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Wild Plants ◽

Acid Oxidation ◽

Mentha Pulegium ◽

Anti Inflammatory

Five ethanol extracts of wild plants from the Casablanca region (Morocco) used in local traditional medicine for the treatment of inflammatory diseases were evaluated for their in vivo topical anti-inflammatory activity (inhibition of carrageenan-induced ear edema in mice) and in vitro antioxidant and antiradical properties (inhibition of linoleic acid oxidation, DPPH radical scavenging). All the extracts showed an anti-inflammatory effect: 300 μg/cm2 provoked edema reductions ranging from 22 to 28%. All the extracts also exerted radical scavenging and/or antioxidant properties, the most active plant being Mentha pulegium L. (Lamiaceae), which contained the highest amount of phenolics (339 mg/g), and flavonoids (16.7 mg/g).

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