Detection of circulating hypoxia-regulated miR-210 in pancreatic adenocarcinoma patients

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 4624-4624
Author(s):  
A. S. Ho ◽  
X. Huang ◽  
H. Cao ◽  
A. C. Koong ◽  
Q. T. Le
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Patients ◽  
Fold Increase ◽  
Adverse Outcomes ◽  
T Test ◽  
Cellular Mechanisms ◽  
C Elegans ◽  
Novel Mirna ◽  
Student’S T ◽  
Student’S T Test

4624 Background: MicroRNAs (miRs) are small non-coding transcripts involved in many cellular mechanisms, including tumorigenesis. miR-210, in particular, has been shown to be induced by hypoxia, over-expressed in several different cancers, and correlated with adverse outcomes in breast cancer. Moreover, since pancreatic adenocarcinomas have been previously shown to be extremely hypoxic, we hypothesized that miR-210 may be elevated in the plasma of these patients compared to non-cancer controls. Here, we compared the circulating plasma levels of miR-210 in pancreatic cancer patients and controls using a novel miRNA extraction approach and quantitative PCR. Methods: Pretreatment EDTA plasma samples were obtained from pancreatic cancer patients and age-matched non-cancer controls. miRNA was extracted from 40ul of plasma and reverse transcribed to cDNA. A known quantity of c. elegans miR-54 was added to the sample as a normalization control. miR-210 and cel-miR-54 were then quantified using TaqMan MicroRNA Assays. The procedure was performed on the initial 11 pairs of age-matched pancreatic cancer patients and non- cancer controls, then validated with a second cohort of 12 pancreatic cancer patients and 11 controls. Results: miR-210 was reliably detected and quantified in small amounts of plasma using the approach developed in our study. There is a statistically significant four-fold increase of mir-210 expression in pancreatic cancer patients compared to normal controls (Student's t-test, p <0.0001). This difference was confirmed in the validation group (Student's t-test, p<0.05). Conclusions: Circulating miR-210 levels can be readily measured from a small quantity of plasma using a novel extraction method. Its expression is significantly higher in the blood of pancreatic cancer patients compared to controls and may potentially serve as a useful biomarker for pancreatic cancer diagnosis. No significant financial relationships to disclose.

The requirement of fentanyl patch in terminally ill patients with cancer.

2016 ◽  
Vol 34 (26_suppl) ◽  
pp. 202-202
Author(s):  
Naoki Shimada ◽  
Aya Watanabe ◽  
Hiroto Ishiki ◽  
Tsukuru Chiba ◽  
Junya Kinkawa ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Pain ◽  
Cancer Patients ◽  
Terminally Ill ◽  
Mixed Effect Model ◽  
Fentanyl Patch ◽  
Mixed Effect ◽  
Effect Model ◽  
Student’S T ◽  
Student’S T Test

202 Background: Cancer pain is the most important problem to overcome in terminally ill cancer patients (TICPs). Recently, the fentanyl patch (FP) has been widely used for the treatment of cancer pain. However, the FP requirement largely varies in patients. The purpose of this study was to identify the determinants of the FP requirement in TICPs and propose effective pain relief using a FP. Methods: In a retrospective chart review, we investigated cancer patients who were admitted in our hospital from April 2012 to July 2015 and used FP until their deaths. We examined the time course of FP use in TICPs until death. We used descriptive statistics and a mixed effect model. The primary endpoint of this study was the final dose of FP use (FDFP). Results: Eighty patients were included the analysis. The clinical characteristics were as follows: age (median, range): 64.5 (29-88) years; male/female ratio: 40/40; primary tumor sites: pancreas (n = 33), digestive tract (n = 12), liver and bile duct (n = 7), ovary and uterus (n = 6), hematologic (n = 5), lung (n = 4), head and neck (n = 4), breast (n = 3), and other (n = 6). FDFP was inversely correlated with age (R = -0.272, P < 0.05; Spearman test). The FDFP (mean ± SD) administered in the patients was 3.06 ± 3.20 mg/day (Male/Female: 3.83 ± 4.06/2.29 ± 1.81 mg/day). The FDFP was 4.36 ± 4.32/2.15 ± 1.64 mg/day in patients with/without pancreatic cancer. FDFP was significantly higher in male than in female patients (P < 0.05; student’s t test), and also significantly higher in patients with pancreatic cancer than in patients without pancreatic cancer (P < 0.005; student’s t test). In pancreatic cancer patients, the FP adjustments were more frequent in the last 60 days of life than in patients with other malignancies (P for interaction < 0.001; mixed effect model). Conclusions: We found that younger age, male sex, and pancreatic cancer were risk factors for higher requirement of FP in TICPs. TICPs with pancreatic cancer in particular required more frequent adjustment of FP near death. We should consider quick titration of fentanyl in patients with pancreatic cancer because the pain-relieving action of FP requires time.

scholarly journals Screening and Validation of Independent Predictors of Poor Survival in Pancreatic Cancer

2021 ◽  
Vol 27 ◽  
Author(s):  
Shui Liu ◽  
Yan Cai ◽  
E. Changyong ◽  
Jiyao Sheng ◽  
Xuewen Zhang
Keyword(s):  
Pancreatic Cancer ◽  
Cox Regression ◽  
Clinical Stage ◽  
T Test ◽  
Functional Similarity ◽  
Hub Genes ◽  
Vital Status ◽  
Student’S T ◽  
One Way Anova ◽  
Student’S T Test

Pancreatic cancer is a digestive system malignant tumor with high mortality and poor prognosis, but the mechanisms of progression remain unclear in pancreatic cancer. It’s necessary to identify the hub genes in pancreatic cancer and explore the novel potential predictors in the prognosis of pancreatic cancer. We downloaded two mRNA expression profiles from Gene Expression Omnibus and The Cancer Genome Atlas Pancreatic Cancer (TCGA-PAAD) datasets to screen the commonly differentially expressed genes in pancreatic cancer by limma package in R. Subsequently, measurement of the functional similarity among the 38 DEGs in common was performed to identify the hub genes using GOSemSim package. Then, survival analysis and Cox regression were applied to explore prognosis-related hub genes using the survival package. Statistics analysis by two-tailed Student’s t-test or one-way based on TCGA-PAAD datasets and qPCR detection in clinical samples were performed to explore the correlations between expression of hub genes in pancreatic cancer tissues and clinical parameters. Based on integrated analysis of TCGA and GEO datasets, we screened 38 DEGs in common, which were all up-regulated. The functional similarity results showed that 10 DEGs including TSPAN1, MSLN, C1orf116, PKP3, CEACAM6, BAIAP2L1, PPL, RAB25, ERBB3, and AP1M2 in the DEGs in common, which had the higher average functional similarity, were considered as the hub genes. Survival analysis results and Cox regression analysis showed that TSPAN1, CEACAM6, as well as ERBB3 were all associated with poor overall survival of PC. qPCR results showed that the expression levels of TSPAN1 and ERBB3 were significantly upregulated in the PC tissues. The statistical analysis results revealed that TSPAN1 expression correlated significantly with histologic grade, T stage, clinical stage, and vital status by two-tailed Student’s t-test or one-way ANOVA; ERBB3 expression correlated significantly with T stage, clinical stage, and vital status by two-tailed Student’s t-test or one-way ANOVA. We found that TSPAN1 and ERBB3 could be independent predictors of poor survival in pancreatic cancer.

scholarly journals Nurses' leadership styles in the icu: association with personal and professional profile and workload

2009 ◽  
Vol 17 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Alexandre Pazetto Balsanelli ◽  
Isabel Cristina Kowal Olm Cunha ◽  
Iveth Yamaguchi Whitaker
Keyword(s):  
Leadership Styles ◽  
Leadership Style ◽  
T Test ◽  
Descriptive Statistics ◽  
Nursing Workload ◽  
Comparison Test ◽  
Multiple Comparison Test ◽  
Professional Profile ◽  
Student’S T ◽  
Student’S T Test

This study aims to explore the association between nurses' leadership styles and personal and professional nursing profile and workload. The sample consisted of seven nurses and seven nursing technicians who were grouped into pairs. At the end of three months, nurses were queried regarding what leadership style would be adopted when the nursing technician under their evaluation delivered care to patients admitted to the ICU. Relevant data was analyzed by applying descriptive statistics, Tukey's multiple comparison test and Student's t-test (p< 0.05). Nursing workload reached 80.1% on average. The personal and professional profile variables did not show any relation with the leadership styles chosen by nurses (p>0.05). The determine, persuade, and share leadership styles prevailed. However, whenever the nursing workload peaked, the determine and persuade styles were used (p<0.05).

scholarly journals Intravesical prostaglandin e2 effectiveness in the prevention of urinary retention after transvaginal reconstruction of the pubo-cervical fascia and short arm sling according to Lahodny: a prospective randomized clinical trial

2010 ◽  
Vol 14 (1) ◽  
pp. 15 ◽  
Author(s):  
G. QUADRI ◽  
N. NATALE ◽  
C. SPREAFICO ◽  
C. BELLONI ◽  
D. BARISANI ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Urinary Retention ◽  
Study Groups ◽  
T Test ◽  
Control Group ◽  
Fisher Exact Test ◽  
Test Results ◽  
Exact Test ◽  
Student’S T ◽  
Student’S T Test

Intravesical prostaglandin E2 is effective in the recovery of spontaneous voiding after transvaginal reconstruction of the pubocervical fascia and short arm sling according to Lahodny. The aim of the study was to compare the effects of intravesical prostaglandin E2 in the prevention of urinary retention after transvaginal reconstruction of the pubocervical fascia and short arm sling according to Lahodny. STUDY DESIGN: From November 1996 to June 1999 fifty women underwent the Lahodny procedure for moderate/severe cystocele and stress urinary incontinence. Women were randomly assigned to 1 of the 2 study groups: intravesical prostaglandin E2 versus controls. Data obtained were analyzed with the Student t test and the Fisher exact test. RESULTS: Two patients of the treatment group had to be excluded from the study, one because of the wrong measurement of the post-voidal residual volume and another due to a fastidious burning sensation which appeared immediately after prostaglandin instillation and required the suspension of the treatment. No other side effects such as nausea, vomiting, diarrhea or hyperthermia were observed. Patients who underwent the prostaglandin E2 treatment showed a recovery of spontaneous voiding after 7.9&plusmn;6.7 days, whereas this interval was significantly longer in the control group, being 12.9&plusmn;9.7 days (p=0.04, Two tailed Unpaired Student's T test). CONCLUSION: The effectiveness and the low associated morbidity mark the treatment with intravesical prostaglandin E2 useful in the recovery of normal voiding after transvaginal pubocervical fascia reconstruction and short arm sling with the procedure according to Lahodny.

scholarly journals Study of Relationship of Anemia among Edentulous Patients

2018 ◽  
Vol 5 (2) ◽  
pp. 105-108
Author(s):  
Lijo Isaac ◽  
A. P. Nirmal Raj ◽  
Reshma Karkera ◽  
R Naveen Reddy
Keyword(s):  
Nutritional Status ◽  
T Test ◽  
Dental Status ◽  
Adult Patients ◽  
Control Group ◽  
Age Group ◽  
Edentulous Patients ◽  
Student’S T ◽  
Relationship Of ◽  
Student’S T Test

Very little studies were done on relationship of the dental status and the nutritional status. The present study was done to study relation between edentulism and the presence of anemia. The study was included of 46 adult patients with edentulism and same numbers of patients were taken as controls. The results were tabulated and analyzed with the help of IBM SPSS statistics 20 using student’s t test. The hemoglobin levels were lower in the edentulous patients that that of the control group. The present study had shown that the nutritional status were poor resulting in anemia in case of edentulous patients as compared to control group with the same age group.  

scholarly journals The influence of proteinuria on tubular transport of Na+, K+, CL

2002 ◽  
Vol 130 (3-4) ◽  
pp. 64-67
Author(s):  
Dejan Petrovic ◽  
Radmila Obrenovic ◽  
Mileta Poskurica ◽  
Biljana Stojimirovic
Keyword(s):  
Statistical Analysis ◽  
Creatinine Clearance ◽  
Significant Influence ◽  
Fractional Excretion ◽  
T Test ◽  
The Third ◽  
Tubular Transport ◽  
Student’S T ◽  
Student’S T Test

Functional and structural damages of tubulointerstitium are caused by proteinuria. The aim of this study was to assess the influence of different proteinuria levels on Na+, K+, Cl tubular transport. We examined 50 patients (24 males, 26 females), mean age 46.50 ? 13.08 years, with mean creati-nine clearence of 87.29 ? 31.17 mL/min. They were separated in three groups depending on proteinuria value. The first group with proteinuria less than 0.3 g/24h included 19 persons (7 males, 12 females), mean age 45.12 ? 13.28 years, with mean creatinine clearance of 94.27 ? 34.70 mL/min. The second group of 18 patients (8 males, 10 females), mean age 45.39 ? 12.64 years had proteinuria of 0.3-3,0 g/24h and mean creatinine clearance of 90.07 ? 31.89 mL/min. The third group had proteinuria level higher than 3.0g/24h and mean creatinine clearance of 73.25 ? 20.44 mL/min. It included 13 patients (9 males, 4 females), mean age 50.08 ? 13.73 years. As a parameter of proteinuria influence on tubular transport of Na+, K+ and Cl-, fractional excretion of these electrolytes, was studied. Student's T test, Mann Whitney U test and c2 test were used for statistical analysis. No statistically significant influence of proteinuria was found on Na+, K+ and Cl tubular transport.

scholarly journals Πρόγνωση ασθενών με κακοήθεια που εισάγονται στη μονάδα εντατικής θεραπείας

2013 ◽  
Author(s):  
Σουσάνα Ανίσογλου
Keyword(s):  
Standard Deviation ◽  
Performance Status ◽  
T Test ◽  
Apache Ii ◽  
Saps Ii ◽  
Student’S T ◽  
Student’S T Test

ΣΚΟΠΟΣ: Η συλλογή, ανάλυση και επεξεργασία στοιχείων ογκολογικών ασθενών που νοσηλεύθηκαν στη Μονάδα Εντατικής Θεραπείας (Μ.Ε.Θ.) και η συσχέτισή τους με συγκεκριμένους πιθανούς παράγοντες κινδύνου .ΥΛΙΚΟ: Πρόκειται για μία προοπτική μελέτη παρατήρησης (prospective observational) σε δείγμα 125 ογκολογικών ασθενών που νοσηλεύθηκαν στη Μ.Ε.Θ. κατά την τελευταία διετία.ΜΕΘΟΔΟΣ: Αξιολογήθηκαν επιδημιολογικοί, κλινικοί και λειτουργικοί παράγοντες. Ειδικότερα ελέγχθηκαν η πρωτοπαθής νεοπλασματική νόσος, η ηλικία, η παρουσία στεφανιαίας νόσου, χρόνιας αποφρακτικής πνευμονοπάθειας, σακχαρώδους διαβήτη, νεφρικής ανεπάρκειας, ο δείκτης μάζας σώματος, η εφαρμογή χημειοθεραπείας ή και ακτινοθεραπείας, η διάρκεια νοσηλείας, τα score βαρύτητας (APACHE II, SAPS II, SOFA) και η έκβαση (εξιτήριο ή θάνατος) και έγιναν συσχετίσεις με τα προηγούμενα στοιχεία. Χρησιμοποιήθηκε το στατιστικό λογισμικό πακέτο SPSS 17.0 (SPSS, Chicago, IL, USA) για Windows. Οι συνεχείς μεταβλητές παρουσιάζονται ως μέσοι όροι + τυπική απόκλιση (mean + standard deviation). Για τη σύγκριση των μέσων όρων μεταξύ δύο ομάδων έγινε χρήση του student’s t-test, εφόσον οι μεταβλητές ακολουθούσαν την κανονική κατανομή και του Mann-Whitney U test στην αντίθετη περίπτωση. Για την ανάδειξη συσχετίσεων μεταξύ ποιοτικών μεταβλητών έγινε χρήση του x2 test.ΑΠΟΤΕΛΕΣΜΑΤΑ: Η θνητότητα ανήλθε στο 48.8%. Στατιστικά σημαντικοί παράγοντες κακής έκβασης κατά την εισαγωγή του ασθενούς στη ΜΕΘ ήταν τα score βαρύτητας (APACHE II, SAPS II, SOFA), το πτωχό performance status και τα συνυπάρχοντα σοβαρά νοσήματα. Στατιστικά σημαντικοί παράγοντες κακής έκβασης κατά τη διάρκεια νοσηλείας του ασθενούς στη Μ.Ε.Θ. ήταν η διάρκεια του μηχανικού αερισμού, η χρήση αγγειοσυσπαστικών, η πολυοργανική ανεπάρκεια και η σηπτική κατάσταση. Από τις εργαστηριακές εξετάσεις στατιστικά σημαντικές ήταν ο χαμηλός αριθμός αιμοπεταλίων και η θετική αιμοκαλλιέργεια. Ο στατιστικός έλεγχος αξιολόγησης κατά Hosmer Lemeshow παρά τον σχετικά μικρό αριθμό του δείγματος επιβεβαίωσε τη χρησιμότητα των APACHE II, SAPS II, SOFA ως προγνωστικών μοντέλων.ΣΥΜΠΕΡΑΣΜΑΤΑ: Η πρόγνωση ογκολογικών ασθενών που εισάγονται στη ΜΕΘ είναι μέτρια. Υπάρχουν σαφείς προγνωστικοί παράγοντες κινδύνου που μπορούν να συνθέσουν ένα είδος προγνωστικού μοντέλου, ωστόσο απαιτούνται περαιτέρω πολυκεντρικές μελέτες με προοπτικό χαρακτήρα σε μεγαλύτερο αριθμό ασθενών.

On the Use of Student's t-Test in an Asymmetrical Population

Biometrika ◽  
1949 ◽  
Vol 36 (3/4) ◽  
pp. 426
Author(s):  
S. G. Ghurye
Keyword(s):  
T Test ◽  
Student’S T ◽  
Student’S T Test
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