Delivery Systems for Proteins and Peptides

2019 ◽  
pp. 145-169
Author(s):  
Sougata Jana ◽  
Arijit Gandhi ◽  
Kalyan Kumar Sen
Keyword(s):  
Delivery Systems ◽  
Proteins And Peptides

scholarly journals Recent Advances in Encapsulation, Protection, and Oral Delivery of Bioactive Proteins and Peptides using Colloidal Systems

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1161 ◽  
Author(s):  
Sarah L. Perry ◽  
David Julian McClements
Keyword(s):  
Ace Inhibitors ◽  
Oral Delivery ◽  
Colloidal Particles ◽  
Functional Performance ◽  
Delivery Systems ◽  
Colloidal Systems ◽  
Adverse Conditions ◽  
Bioactive Proteins ◽  
Food Applications ◽  
Proteins And Peptides

There are many areas in medicine and industry where it would be advantageous to orally deliver bioactive proteins and peptides (BPPs), including ACE inhibitors, antimicrobials, antioxidants, hormones, enzymes, and vaccines. A major challenge in this area is that many BPPs degrade during storage of the product or during passage through the human gut, thereby losing their activity. Moreover, many BPPs have undesirable taste profiles (such as bitterness or astringency), which makes them unpleasant to consume. These challenges can often be overcome by encapsulating them within colloidal particles that protect them from any adverse conditions in their environment, but then release them at the desired site-of-action, which may be inside the gut or body. This article begins with a discussion of BPP characteristics and the hurdles involved in their delivery. It then highlights the characteristics of colloidal particles that can be manipulated to create effective BPP-delivery systems, including particle composition, size, and interfacial properties. The factors impacting the functional performance of colloidal delivery systems are then highlighted, including their loading capacity, encapsulation efficiency, protective properties, retention/release properties, and stability. Different kinds of colloidal delivery systems suitable for encapsulation of BPPs are then reviewed, such as microemulsions, emulsions, solid lipid particles, liposomes, and microgels. Finally, some examples of the use of colloidal delivery systems for delivery of specific BPPs are given, including hormones, enzymes, vaccines, antimicrobials, and ACE inhibitors. An emphasis is on the development of food-grade colloidal delivery systems, which could be used in functional or medical food applications. The knowledge presented should facilitate the design of more effective vehicles for the oral delivery of bioactive proteins and peptides.

scholarly journals Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3002
Author(s):  
Simge Er ◽  
Ushna Laraib ◽  
Rabia Arshad ◽  
Saman Sargazi ◽  
Abbas Rahdar ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Amino Acids ◽  
Amino Acid ◽  
Gene Delivery ◽  
Cost Effective ◽  
Delivery Systems ◽  
Drug Molecules ◽  
Or Gene ◽  
Gold Nanoislands ◽  
Proteins And Peptides

Over various scientific fields in biochemistry, amino acids have been highlighted in research works. Protein, peptide- and amino acid-based drug delivery systems have proficiently transformed nanotechnology via immense flexibility in their features for attaching various drug molecules and biodegradable polymers. In this regard, novel nanostructures including carbon nanotubes, electrospun carbon nanofibers, gold nanoislands, and metal-based nanoparticles have been introduced as nanosensors for accurate detection of these organic compounds. These nanostructures can bind the biological receptor to the sensor surface and increase the surface area of the working electrode, significantly enhancing the biosensor performance. Interestingly, protein-based nanocarriers have also emerged as useful drug and gene delivery platforms. This is important since, despite recent advancements, there are still biological barriers and other obstacles limiting gene and drug delivery efficacy. Currently available strategies for gene therapy are not cost-effective, and they do not deliver the genetic cargo effectively to target sites. With rapid advancements in nanotechnology, novel gene delivery systems are introduced as nonviral vectors such as protein, peptide, and amino acid-based nanostructures. These nano-based delivery platforms can be tailored into functional transformation using proteins and peptides ligands based nanocarriers, usually overexpressed in the specified diseases. The purpose of this review is to shed light on traditional and nanotechnology-based methods to detect amino acids, peptides, and proteins. Furthermore, new insights into the potential of amino protein-based nanoassemblies for targeted drug delivery or gene transfer are presented.

Two-level delivery systems for oral administration of peptides and proteins based on spore capsules of Lycopodium clavatum

2017 ◽  
Vol 5 (37) ◽  
pp. 7711-7720 ◽  
Author(s):  
Natalia Sudareva ◽  
Olga Suvorova ◽  
Natalia Saprykina ◽  
Alexander Vilesov ◽  
Petr Bel'tiukov ◽  
...  
Keyword(s):  
Oral Administration ◽  
Therapeutic Proteins ◽  
Delivery Systems ◽  
Lycopodium Clavatum ◽  
Proteins And Peptides

Two-level delivery systems for oral administration of therapeutic proteins and peptides were developed.

Orally active-targeted drug delivery systems for proteins and peptides

2014 ◽  
Vol 11 (9) ◽  
pp. 1435-1447 ◽  
Author(s):  
Xiuying Li ◽  
Miaorong Yu ◽  
Weiwei Fan ◽  
Yong Gan ◽  
Lars Hovgaard ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Targeted Drug ◽  
Orally Active ◽  
Targeted Drug Delivery Systems ◽  
Proteins And Peptides

scholarly journals Nanomaterials for the Local and Targeted Delivery of Osteoarthritis Drugs

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Parthiban Chinnagounder Periyasamy ◽  
Jeroen C. H. Leijten ◽  
Pieter J. Dijkstra ◽  
Marcel Karperien ◽  
Janine N. Post
Keyword(s):  
Targeted Delivery ◽  
Building Blocks ◽  
Delivery Systems ◽  
Science And Engineering ◽  
Disease Modifying Drugs ◽  
Nanodrug Delivery ◽  
Naturally Occurring ◽  
Current Therapies ◽  
Cartilage Diseases ◽  
Proteins And Peptides

Nanotechnology has found its potential in every possible field of science and engineering. It offers a plethora of options to design tools at the nanometer scale, which can be expected to function more effectively than micro- and macrosystems for specific applications. Although the debate regarding the safety of synthetic nanomaterials for clinical applications endures, it is a promising technology due to its potential to augment current treatments. Various materials such as synthetic polymer, biopolymers, or naturally occurring materials such as proteins and peptides can serve as building blocks for adaptive nanoscale formulations. The choice of materials depends highly on the application. We focus on the use of nanoparticles for the treatment of degenerative cartilage diseases, such as osteoarthritis (OA). Current therapies for OA focus on treating the symptoms rather than modifying the disease. The usefulness of OA disease modifying drugs is hampered by side effects and lack of suitable drug delivery systems that target, deliver, and retain drugs locally. This challenge can be overcome by using nanotechnological formulations. We describe the different nanodrug delivery systems and their potential for cartilage repair. This paper provides the reader basal understanding of nanomaterials and aims at drawing new perspectives on the use of existing nanotechnological formulations for the treatment of osteoarthritis.

scholarly journals Micellar Drug Delivery Systems Based on Natural Biopolymers

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 477
Author(s):  
Leonard Ionut Atanase
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Drug Loading ◽  
Cost Effective ◽  
Delivery Systems ◽  
Micellar Systems ◽  
Micellar Aggregates ◽  
Loading Methods ◽  
Proteins And Peptides

The broad diversity of structures and the presence of numerous functional groups available for chemical modifications represent an enormous advantage for the development of safe, non-toxic, and cost-effective micellar drug delivery systems (DDS) based on natural biopolymers, such as polysaccharides, proteins, and peptides. Different drug-loading methods are used for the preparation of these micellar systems, but it appeared that dialysis is generally recommended, as it avoids the formation of large micellar aggregates. Moreover, the preparation method has an important influence on micellar size, morphology, and drug loading efficiency. The small size allows the passive accumulation of these micellar systems via the permeability and retention effect. Natural biopolymer-based micellar DDS are high-value biomaterials characterized by good compatibility, biodegradability, long blood circulation time, non-toxicity, non-immunogenicity, and high drug loading, and they are biodegraded to non-toxic products that are easily assimilated by the human body. Even if some recent studies reported better antitumoral effects for the micellar DDS based on polysaccharides than for commercial formulations, their clinical use is not yet generalized. This review is focused on the studies from the last decade concerning the preparation as well as the colloidal and biological characterization of micellar DDS based on natural biopolymers.

Techniques For The Preparation of Tissue Samples Containing Injectable Polymer Microcapsules

1985 ◽  
Vol 43 ◽  
pp. 710-711
Author(s):  
G.E. Visscher ◽  
R. L. Robison ◽  
G. J. Argentieri
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Gastrocnemius Muscle ◽  
Degradation Process ◽  
Delivery Systems ◽  
Tissue Reaction ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Injectable Polymer ◽  
Microscopic Techniques

The use of various bioerodable polymers as drug delivery systems has gained considerable interest in recent years. Among some of the shapes used as delivery systems are films, rods and microcapsules. The work presented here will deal with the techniques we have utilized for the analysis of the tissue reaction to and actual biodegradation of injectable microcapsules. This work has utilized light microscopic (LM), transmission (TEM) and scanning (SEM) electron microscopic techniques. The design of our studies has utilized methodology that would; 1. best characterize the actual degradation process without artifacts introduced by fixation procedures and 2. allow for reproducible results.In our studies, the gastrocnemius muscle of the rat was chosen as the injection site. Prior to the injection of microcapsules the skin above the sites was shaved and tattooed for later recognition and recovery. 1.0 cc syringes were loaded with the desired quantity of microcapsules and the vehicle (0.5% hydroxypropylmethycellulose) drawn up. The syringes were agitated to suspend the microcapsules in the injection vehicle.

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