scholarly journals Osteoclast Activity and Subtypes as a Function of Physiology and Pathology—Implications for Future Treatments of Osteoporosis

2011 ◽  
Vol 32 (1) ◽  
pp. 31-63 ◽  
Author(s):  
K. Henriksen ◽  
J. Bollerslev ◽  
V. Everts ◽  
M. A. Karsdal
Keyword(s):  
Bone Resorption ◽  
Bone Quality ◽  
Cell Communication ◽  
Bone Microenvironment ◽  
Cell Type ◽  
Drug Induced ◽  
Current Review ◽  
Heterogeneous Cell Population ◽  
Future Treatments ◽  
Insight Into

Abstract Osteoclasts have traditionally been associated exclusively with catabolic functions that are a prerequisite for bone resorption. However, emerging data suggest that osteoclasts also carry out functions that are important for optimal bone formation and bone quality. Moreover, recent findings indicate that osteoclasts have different subtypes depending on their location, genotype, and possibly in response to drug intervention. The aim of the current review is to describe the subtypes of osteoclasts in four different settings: 1) physiological, in relation to turnover of different bone types; 2) pathological, as exemplified by monogenomic disorders; 3) pathological, as identified by different disorders; and 4) in drug-induced situations. The profiles of these subtypes strongly suggest that these osteoclasts belong to a heterogeneous cell population, namely, a diverse macrophage-associated cell type with bone catabolic and anabolic functions that are dependent on both local and systemic parameters. Further insight into these osteoclast subtypes may be important for understanding cell–cell communication in the bone microenvironment, treatment effects, and ultimately bone quality.

scholarly journals Bisphosphonates

2006 ◽  
Vol 50 (4) ◽  
pp. 735-744 ◽  
Author(s):  
Michael McClung
Keyword(s):  
Bone Resorption ◽  
Bone Loss ◽  
Bone Turnover ◽  
Bone Structure ◽  
Clinical Settings ◽  
Low Bone Mass ◽  
Dosing Regimen ◽  
Drug Induced ◽  
Excellent Safety Profile ◽  
Reduce Fracture Risk

Osteoporosis is the result of bone loss due to an imbalance in bone turnover such that bone resorption exceeds bone formation. Bisphosphonates are potent inhibitors of osteoclast activity that reduce bone turnover and re-establish the balance between bone resorption and formation. In clinical studies, several bisphosphonates prevent bone loss, preserve bone structure, improve bone strength and, in patients with osteoporosis, substantially reduce fracture risk. They are effective in multiple clinical settings including postmenopausal osteoporosis, low bone mass in men and drug-induced bone loss. Intermittent oral dosing and intravenous administration are more convenient than the original daily dosing regimen. These drugs are generally well tolerated and have an excellent safety profile in that serious side effects are uncommon. Potent bisphosphonates are generally the preferred treatment option for most patients with or at risk for osteoporosis.

scholarly journals An insight into the polymeric structures in Asian Palmyra palm (Borassus flabellifer Linn)

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Paulraj Mosae Selvakumar ◽  
Rex Jeya Rajkumar Samdavid Thanapaul
Keyword(s):  
Climatic Conditions ◽  
Palm Tree ◽  
Asian Countries ◽  
Current Review ◽  
Medicinal Value ◽  
Native Tree ◽  
Polymeric Compounds ◽  
Value Add ◽  
Polymeric Structures ◽  
Insight Into

Palmyra Palm (Borassus flabellifer Linn) is a native tree with various ecological, medicinal, economic, and sociological benefits from Asian countries. Palmyra Palm tree-based self-reliant lifestyle and Eco-friendly community living which leads to sustainable development can be called as palmyraculture. For each component, it is the most beneficial species that has economic and medicinal value that could sustain adverse climatic conditions and resist natural calamities. Non-edible, edible, and value add-based uses could be widely categorized into the utility of the plant. Palmyra palm tree is working like a non-stop biochemical factory, creates sugars and many other useful chemicals. They also produce many useful polymeric compounds such as pectin, cellulose, hemicellulose, galactomannan, pentosan, polyphenols, and lignin. The current review gives an insight into the structural, biological and polymeric elements of Asian palmyra. . 

scholarly journals Panoramic stitching of heterogeneous single-cell transcriptomic data

2018 ◽  
Author(s):  
Brian Hie ◽  
Bryan Bryson ◽  
Bonnie Berger
Keyword(s):  
Single Cell ◽  
Cell Types ◽  
Data Sets ◽  
Cell Type ◽  
Data Set ◽  
Wide Range ◽  
Data Set Integration ◽  
Biological Patterns ◽  
Insight Into ◽  
Comprehensive Reference

AbstractResearchers are generating single-cell RNA sequencing (scRNA-seq) profiles of diverse biological systems1–4 and every cell type in the human body.5 Leveraging this data to gain unprecedented insight into biology and disease will require assembling heterogeneous cell populations across multiple experiments, laboratories, and technologies. Although methods for scRNA-seq data integration exist6,7, they often naively merge data sets together even when the data sets have no cell types in common, leading to results that do not correspond to real biological patterns. Here we present Scanorama, inspired by algorithms for panorama stitching, that overcomes the limitations of existing methods to enable accurate, heterogeneous scRNA-seq data set integration. Our strategy identifies and merges the shared cell types among all pairs of data sets and is orders of magnitude faster than existing techniques. We use Scanorama to combine 105,476 cells from 26 diverse scRNA-seq experiments across 9 different technologies into a single comprehensive reference, demonstrating how Scanorama can be used to obtain a more complete picture of cellular function across a wide range of scRNA-seq experiments.

Drug-induced alterations in Mg2+ homoeostasis

2012 ◽  
Vol 123 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Anke L. Lameris ◽  
Leo A. Monnens ◽  
René J. Bindels ◽  
Joost G. J. Hoenderop
Keyword(s):  
Adverse Effects ◽  
Molecular Mechanisms ◽  
Growth Factor Receptor ◽  
Calcineurin Inhibitors ◽  
Drug Induced ◽  
Neuromuscular Abnormalities ◽  
Broad Variety ◽  
Epidermal Growth ◽  
High Doses ◽  
Insight Into

Magnesium (Mg2+) balance is tightly regulated by the concerted actions of the intestine, bone and kidneys. This balance can be disturbed by a broad variety of drugs. Diuretics, modulators of the EGFR (epidermal growth factor receptor), proton pump inhibitors, antimicrobials, calcineurin inhibitors and cytostatics may all cause hypomagnesaemia, potentially leading to tetany, seizures and cardiac arrhythmias. Conversely, high doses of Mg2+ salts, frequently administered as an antacid or a laxative, may lead to hypermagnesaemia causing various cardiovascular and neuromuscular abnormalities. A better understanding of the molecular mechanisms underlying the adverse effects of these medications on Mg2+ balance will indicate ways of prevention and treatment of these adverse effects and could potentially provide more insight into Mg2+ homoeostasis.

scholarly journals Controlled Construction of Stable Network Structure Composed of Honeycomb-Shaped Microhydrogels

Life ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 38 ◽  
Author(s):  
Masayuki Hayakawa ◽  
Satoshi Umeyama ◽  
Ken Nagai ◽  
Hiroaki Onoe ◽  
Masahiro Takinoue
Keyword(s):  
Cell Communication ◽  
Model Systems ◽  
Stable Component ◽  
Photosensitive Polymer ◽  
A Cell ◽  
External Stimuli ◽  
Structural Instabilities ◽  
Insight Into ◽  
Stable Network ◽  
Microfluidic Technique

Recently, the construction of models for multicellular systems such as tissues has been attracting great interest. These model systems are expected to reproduce a cell communication network and provide insight into complicated functions in living systems./Such network structures have mainly been modelled using a droplet and a vesicle. However, in the droplet and vesicle network, there are difficulties attributed to structural instabilities due to external stimuli and perturbations. Thus, the fabrication of a network composed of a stable component such as hydrogel is desired. In this article, the construction of a stable network composed of honeycomb-shaped microhydrogels is described. We produced the microhydrogel network using a centrifugal microfluidic technique and a photosensitive polymer. In the network, densely packed honeycomb-shaped microhydrogels were observed. Additionally, we successfully controlled the degree of packing of microhydrogels in the network by changing the centrifugal force. We believe that our stable network will contribute to the study of cell communication in multicellular systems.

scholarly journals Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling

2018 ◽  
Vol 217 (3) ◽  
pp. 1129-1142 ◽  
Author(s):  
Frederik Johannes Verweij ◽  
Maarten P. Bebelman ◽  
Connie R. Jimenez ◽  
Juan J. Garcia-Vallejo ◽  
Hans Janssen ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Single Cells ◽  
Cell Communication ◽  
Exosome Biogenesis ◽  
Real Time Visualization ◽  
Target Membrane ◽  
Syntaxin 4 ◽  
Optical Reporters ◽  
Druggable Targets ◽  
Insight Into

Exosomes are small endosome-derived extracellular vesicles implicated in cell–cell communication and are secreted by living cells when multivesicular bodies (MVBs) fuse with the plasma membrane (PM). Current techniques to study exosome physiology are based on isolation procedures after secretion, precluding direct and dynamic insight into the mechanics of exosome biogenesis and the regulation of their release. In this study, we propose real-time visualization of MVB–PM fusion to overcome these limitations. We designed tetraspanin-based pH-sensitive optical reporters that detect MVB–PM fusion using live total internal reflection fluorescence and dynamic correlative light–electron microscopy. Quantitative analysis demonstrates that MVB–PM fusion frequency is reduced by depleting the target membrane SNAREs SNAP23 and syntaxin-4 but also can be induced in single cells by stimulation of the histamine H1 receptor (H1HR). Interestingly, activation of H1R1 in HeLa cells increases Ser110 phosphorylation of SNAP23, promoting MVB–PM fusion and the release of CD63-enriched exosomes. Using this single-cell resolution approach, we highlight the modulatory dynamics of MVB exocytosis that will help to increase our understanding of exosome physiology and identify druggable targets in exosome-associated pathologies.

scholarly journals Northstar enables automatic classification of known and novel cell types from tumor samples

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fabio Zanini ◽  
Bojk A. Berghuis ◽  
Robert C. Jones ◽  
Benedetta Nicolis di Robilant ◽  
Rachel Yuan Nong ◽  
...  
Keyword(s):  
Cell Types ◽  
Neoplastic Cell ◽  
Computational Approach ◽  
Pancreatic Tumors ◽  
Published Data ◽  
Cell Type ◽  
Disease Heterogeneity ◽  
Manual Task ◽  
Insight Into

Abstract Single cell transcriptomics is revolutionising our understanding of tissue and disease heterogeneity, yet cell type identification remains a partially manual task. Published algorithms for automatic cell annotation are limited to known cell types and fail to capture novel populations, especially cancer cells. We developed northstar, a computational approach to classify thousands of cells based on published data within seconds while simultaneously identifying and highlighting new cell states such as malignancies. We tested northstar on data from glioblastoma, melanoma, and seven different healthy tissues and obtained high accuracy and robustness. We collected eleven pancreatic tumors and identified three shared and five private neoplastic cell populations, offering insight into the origins of neuroendocrine and exocrine tumors. Northstar is a useful tool to assign known and novel cell type and states in the age of cell atlases.

scholarly journals Drug-Induced Sleep Endoscopy: Clinical Application and Surgical Outcomes

Healthcare ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 100 ◽  
Author(s):  
Vito ◽  
Cammaroto ◽  
Chong ◽  
Carrasco-Llatas ◽  
Vicini
Keyword(s):  
Surgical Outcomes ◽  
Acoustic Analysis ◽  
Three Dimensional ◽  
Upper Airway ◽  
Sleep Endoscopy ◽  
Drug Induced ◽  
Drug Induced Sleep Endoscopy ◽  
Therapeutic Decision Making ◽  
Open Question ◽  
Insight Into

The visualization of the level and pattern of apnea and hypopnea events is of pivotal importance in the diagnosis and therapeutic decision-making for sleep-disordered breathing (SDB). There are numerous techniques available to assess upper airway obstruction, which include imaging, acoustic analysis, pressure transducer recording, and endoscopic evaluation. Drug-induced sleep endoscopy (DISE) is a diagnostic tool that allows the dynamic, three-dimensional evaluation of the patterns of vibration and collapse of the upper airway of SDB patients. DISE may change the initial surgical planning in a high percentage of cases. A universally accepted and methodologically standardized DISE could provide significant insight into its role to improve surgical outcomes. However, up to now the ideal DISE protocol remains an open question.

scholarly journals Perioperative Organ Injury

2013 ◽  
Vol 119 (6) ◽  
pp. 1474-1489 ◽  
Author(s):  
Karsten Bartels ◽  
Jörn Karhausen ◽  
Eric T. Clambey ◽  
Almut Grenz ◽  
Holger K. Eltzschig
Keyword(s):  
Distress Syndrome ◽  
Kidney Injury ◽  
Organ Injury ◽  
Therapeutic Approaches ◽  
The Past ◽  
Novel Treatment ◽  
Current Review ◽  
Common Causes ◽  
Treatment Concepts ◽  
Insight Into

Abstract Despite the fact that a surgical procedure may have been performed for the appropriate indication and in a technically perfect manner, patients are threatened by perioperative organ injury. For example, stroke, myocardial infarction, acute respiratory distress syndrome, acute kidney injury, or acute gut injury are among the most common causes for morbidity and mortality in surgical patients. In the current review, the authors discuss the pathogenesis of perioperative organ injury, and provide select examples for novel treatment concepts that have emerged over the past decade. Indeed, the authors are of the opinion that research to provide mechanistic insight into acute organ injury and identification of novel therapeutic approaches for the prevention or treatment of perioperative organ injury represent the most important opportunity to improve outcomes of anesthesia and surgery.

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