Protein synthesis during Ilyanassa development: effect of the polar lobe

Development ◽  
1973 ◽  
Vol 29 (2) ◽  
pp. 267-281
Author(s):  
G. Teitelman
Keyword(s):  
Protein Synthesis ◽  
Normal Development ◽  
Disc Electrophoresis ◽  
Developmental Differences ◽  
Protein Solutions ◽  
Polar Lobe ◽  
Stages Of Development ◽  
Morphogenetic Factors ◽  
Slow Moving ◽  
The One

Removal of the polar lobe at the trefoil stage of the first cleavage division of Ilyanassa embryos causes abnormalities much later in development. To determine if the developmental differences between normal and delobed embryos were reflected in alterations in protein synthesis and at what stages of development these become evident, protein solutions were separated by disc electrophoresis on basic acrylamide gels. For the analysis of the newly synthesized proteins, two protein samples, one labelled with 14C and the other with 3H, were combined in the same electrophoretic column. Each was prepared from normal embryos or lobeless embryos at different stages of development. The distribution of the two groups of differentially labelled proteins was compared by a determination, for each fraction, of the ratio of the normalized 3H/14C counts for that particular fraction (R = 3H/14C). The plot of R versus fraction number was studied for various combinations of samples. During normal development the profile of labelled proteins remains unchanged until the onset of visible differentiation. At this stage, around day 4 of development, there are changes in biosynthesis revealed by a greater emphasis on the synthesis of slow moving proteins. The profile of labelled proteins of lobeless embryos remains unchanged up to the 5th day of development. This result is correlated with the absence, in the lobeless embryos, of many of the visible differentiations. Preliminary studies revealed that the spectrum of labelled proteins of the polar lobe is identical to the one present in lobeless embryos and in normal embryos in early stages of development. This suggests the possibility that the morphogenetic factors associated with the polar lobe are not among the newly synthesized proteins. A hypothesis is presented to account for the effects on morphogenesis and protein synthesis which are produced by removal of the polar lobe.

The study of Apara (placenta) and its correlation with the Prakriti and Weight of newborn

2017 ◽  
Vol 2 (05) ◽  
Author(s):  
Apurva Dhakulkar ◽  
Sachin Khedikar
Keyword(s):  
Basic Concept ◽  
Normal Development ◽  
Unique Contribution ◽  
Multiple Functions ◽  
Intrauterine Life ◽  
Foetal Growth ◽  
Healthy Pregnancy ◽  
The One

The placenta is the most important and the only organ between mother and foetus serving multiple functions like endocrinal, respiratory and metabolic. Normal development of the placenta is the one of the important requirements for a healthy pregnancy, regulating foetal growth and foetal health so that formation of the healthy progeny. In cotemporary sciences many researches are going in the aim of formation of healthy progeny. Apara is the vital feature related to Garbha Sharira in Ayurveda, but its description is in a Sutra form. Hence this study is an humble attempt to explore the concept of ‘Ayurveda Garbha Sharira’ by studying the description of Apara trying to establishment of the assessment of correlation between features of placenta with that of Prakriti is more instinct for this study. Prakriti is the basic concept of Ayurveda. It forms in intrauterine life by combination of Shukra and Shonita. However we do not find direct references of Apara relation with the Prakriti of newborn in Ayurveda. This study intends to fill in the lacunae of both the disciplines by knowledge of integration. If this study establishes some parameters based on the characteristics of Apara with weight and Prakriti of newborn then it will be the unique contribution for Ayurveda Garbha Sharira.

Effect of microbial isolates on microbial yield estimation in RUSITEC system

1998 ◽  
Vol 22 ◽  
pp. 306-308
Author(s):  
M. D. Carro ◽  
E. L. Miller
Keyword(s):  
Protein Synthesis ◽  
Liquid Phase ◽  
Solid Phase ◽  
Liquid Fraction ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Continuous Culture System ◽  
The One

The estimation of rumen microbial protein synthesis is one of the main points in the nitrogen (N)-rationing systems for ruminants, as microbial protein provides proportionately 0.4 to 0.9 of amino acids entering the small intestine in ruminants receiving conventional diets (Russell et al., 1992). Methods of estimating microbial protein synthesis rely on marker techniques in which a particular microbial constituent is related to the microbial N content. Marker : N values have generally been established in mixed bacteria isolated from the liquid fraction of rumen digesta and it has been assumed that the same relationship holds in the total population leaving the rumen (Merry and McAllan, 1983). However, several studies have demonstrated differences in composition between solid-associated (SAB) and fluid-associated bacteria in vivo (Legay-Carmier and Bauchart, 1989) and in vitro (Molina Alcaide et al, 1996), as well in marker : N values (Pérez et al., 1996). This problem could be more pronounced in the in vitro semi-continuous culture system RUSITEC, in which there are three well defined components (a free liquid phase, a liquid phase associated with the solid phase and a solid phase), each one having associated microbial populations.The objective of this experiment was to investigate the effect of using different bacterial isolates (BI) on the estimation of microbial production of four different diets in RUSITEC (Czerkawski and Breckenridge, 1977), using (15NH4)2 SO4 as microbial marker, and to assess what effects any differences would have on the comparison of microbial protein synthesis between diets.This study was conducted in conjunction with an in vitro experiment described by Carro and Miller (1997). Two 14-day incubation trials were carried out with the rumen simulation technique RUSITEC (Czerkawski and Breckenridge, 1977). The general incubation procedure was the one described by Czerkawski and Breckenridge (1977) and more details about the procedures of this experiment are given elsewhere (Carro and Miller, 1997).

scholarly journals Морфологічні зміни тимусу курчат при різних схемах антибактеріальної терапії

2017 ◽  
Vol 19 (73) ◽  
pp. 11-18
Author(s):  
L.Y. Lyzogub
Keyword(s):  
Antibacterial Agents ◽  
Normal Development ◽  
Negative Impact ◽  
Preventive Treatment ◽  
Antibacterial Drug ◽  
Antibacterial Drugs ◽  
Main Thing ◽  
The Moment ◽  
The One ◽  
Positive Effect

The maximum number of products at the lowest cost – is the main thing, what modern poultry industry can be characterized. One of the most pressing problems at the moment is early antibiotic therapy and the associated safety of young poultry early age. In this regard, of particular interest for this study is how the most widely used antibacterial drugs effects on the one of key organs of the immune system – thymus. The article presents the data obtained in the course of the experiment, which determines how preventive treatment schemes, that includes usage single and multiple antibacterial drugs, influenced on the morphological status of the chickens thymus. We selected scheme and a combination of antibacterial drugs have not been studied previously. That was compared with the usage of probiotic Bolmol at the same treatment scheme. A comparison of morpho-functional state of the thymus of chickens 15-, 22- and 41-days-old, when applying several treatments schemes. The schemes of treatment included: the usage of one or two antibiotics during the cycle, compared with the usage of probiotic. We made the conclusions about the absence of the negative impact of selected drugs on the morphofunctional state of the thymus and the positive effect of probiotic «Bolmol». The morphometric parameters of the cortex and medulla in the lobules of the thymus of chicks 15-day-old, taking as an antibacterial drug probiotic Bolmol, were not significantly different from control values and were statistically significant when compared with chicks who used antibiotics: the width of the cortical area was on 15% and 29.09% wider then chickens receiving antibiotic treatment. This indicates a positive effect of probiotic Bolmol compared with antibacterial agents which were chosen. Thus, these data demonstrate that the use of probiotics in cyclic schemes of antibiotic prophylaxis contribute to the normal development of the thymus, which in turn leads to increased resistance and preservation of poultry. Was given the substantiation about safely usage of cyclic schemes of antibacterial drugs in the event of objective necessity.

Cytoplasmic regulation of translation during Ilyanassa embryogenesis

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 263-269
Author(s):  
Collier JR
Keyword(s):  
Protein Synthesis ◽  
Actinomycin D ◽  
Specific Gene ◽  
Early Cleavage ◽  
Polar Lobe ◽  
Nuclear Activity ◽  
Two Dimensional Electrophoresis ◽  
Regulation Of Translation ◽  
Maternal Mrnas ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of labeled proteins and hybridization of mRNAs with specific gene probes was demonstrate changes in protein synthesis, and the of actin maternal mRNAs during the early development isolated polar lobe was used as a nucleus-free egg the regulation of translation. It was shown that actin present in the unfertilized egg and are therefore are inactive during early cleavage, and are translated lobeless, actinomycin D-treated blastulae and in that have been aged for 24h. Thus, the activation of controlled by cytoplasmic factors that function cleavage and nuclear activity. I suggest that the cytoplasmic clock determines when maternal mRNAs are that this clock is made and set running during protein synthesis that occurred during early cleavage also involve the diminution of some early cleavage suggested that this diminution is controlled by localized in the blastomeres of the lobeless egg but polar lobe.

scholarly journals Molecular Replication

1985 ◽  
Vol 112 ◽  
pp. 199-200
Author(s):  
Leslie E. Orgel
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
Life Form ◽  
Similar Process ◽  
The Earth ◽  
Early Appearance ◽  
Living Organisms ◽  
The One ◽  
Molecular Replication

The replication of DNA is the process by means of which genetic information is passed from one generation to the next in all living organisms. It is widely believed that a similar process must have become important early in the development of life on the Earth, either before or at the same time as the evolution of protein synthesis. The evolution of any life-form based on familiar chemistry would seem to require the early appearance of some type of residue-by-residue replication, although it might be very different in detail from the one we know.

scholarly journals Loss of Innocence: Albert Moll, Sigmund Freud and the Invention of Childhood Sexuality Around 1900

2012 ◽  
Vol 56 (2) ◽  
pp. 156-183 ◽  
Author(s):  
Lutz D.H. Sauerteig
Keyword(s):  
Nineteenth Century ◽  
Twentieth Century ◽  
Sigmund Freud ◽  
Normal Development ◽  
The Other ◽  
Sexual Experiences ◽  
Childhood Sexuality ◽  
Adults And Children ◽  
Formed Part ◽  
The One

AbstractThis paper analyses how, prior to the work of Sigmund Freud, an understanding of infant and childhood sexuality emerged during the nineteenth century. Key contributors to the debate were Albert Moll, Max Dessoir and others, as fin-de-siècle artists and writers celebrated a sexualised image of the child. By the beginning of the twentieth century, most paediatricians, sexologists, psychologists, psychiatrists, psychoanalysts and pedagogues agreed that sexuality formed part of a child’s ‘normal’ development. This paper argues that the main disagreements in discourses about childhood sexuality related to different interpretations of children’s sexual experiences. On the one hand stood an explanation that argued for a homology between children’s and adults’ sexual experiences, on the other hand was an understanding that suggested that adults and children had distinct and different experiences. Whereas the homological interpretation was favoured by the majority of commentators, including Moll, Freud, and to some extent also by C.G. Jung, the heterological interpretation was supported by a minority, including childhood psychologist Charlotte Bühler.

Chromatin remodelling and nuclear reprogramming at the onset of embryonic development in mammals

1998 ◽  
Vol 10 (8) ◽  
pp. 573 ◽  
Author(s):  
Jean-Paul Renard
Keyword(s):  
Normal Development ◽  
Blastocyst Stage ◽  
Chromatin Remodelling ◽  
Chromatin Organization ◽  
Nuclear Reprogramming ◽  
Cell Stage ◽  
Donor Nucleus ◽  
The One ◽  
Kinetics Of ◽  
Determining Factor

Two main strategies are used to produce cloned mammals. The first involves the condensation of donor chromatin into chromosomes directly exposed to the recipient cytoplasm, whereas the second leaves the donor nucleus in interphase until the time of the first mitosis. Both strategies, which induce marked changes in chromatin organization, allow full reprogrammation of somatic-differentiated fetal and adult cells. This paper reviews some of the recent data that contribute to our understanding of chromatin remodelling at the onset of normal development, as well as after the introduction of a foreign nucleus into a recipient enucleated oocyte. These data indicate that the coordinated changes in chromatin organization that take place up until the first cellular differentiations at the blastocyst stage are determinants for successful cloning. Although some degree of synchronization between the cell cycle stages of donor and recipient cells is necessary for correct remodelling of a transferred nucleus, the kinetics of remodelling events occurring during the one-cell stage appears to be the determining factor for the normal onset of gene expression.

Role of peptidylarginine deiminase 4 (PAD4) in pig parthenogenetic preimplantation embryonic development

Zygote ◽  
2012 ◽  
Vol 21 (4) ◽  
pp. 385-393
Author(s):  
Manjula Brahmajosyula ◽  
Masashi Miyake
Keyword(s):  
Gene Regulation ◽  
Embryonic Development ◽  
Normal Development ◽  
Preimplantation Embryos ◽  
Peptidylarginine Deiminase ◽  
Stages Of Development ◽  
Developmental Arrest ◽  
Slow Development ◽  
Arginine Modification

SummaryArginine modification to citrulline (citrullination) is catalyzed by peptidylarginine deiminases (PADs) and one of the isomers PAD4 is shown to be involved in the gene regulation. In our previous paper we studied the localization and expression of PAD4 and the target of PAD4 in mammalian gametes and preimplantation embryos. In this study the role of PAD4 was examined in the pig diploid parthenogenetic preimplantation embryonic development. Knockdown of PAD4 by RNAi resulted in delayed development. Inhibition of PAD4 by a potent PAD4 inhibitor Cl-amidine from the time of activation for 24 h resulted in developmental arrest at the first cleavage. Inhibition at the later stages of development resulted in delayed or arrested development. A shorter exposure to Cl-amidine for 6 h at any stage of growth resulted in slow development. Thus, this study suggests that PAD4 activity is essential for the normal development of the embryos.

PROTEIN SYNTHESIS IN DARK-GROWN BEAN LEAVES

1965 ◽  
Vol 43 (7) ◽  
pp. 817-824 ◽  
Author(s):  
David Racusen ◽  
Murray Foote
Keyword(s):  
Protein Synthesis ◽  
Soluble Protein ◽  
Specific Activity ◽  
Disc Electrophoresis ◽  
Protein Zone ◽  
Bean Leaves

RuDP carboxylase was present in 4-day-old, dark-grown bean leaves. The enzyme increased during subsequent dark growth and in 11 days reached one-third of the specific activity of a light-grown control. Preparative disc electrophoresis of the soluble protein revealed a zone symmetrical with RuDP carboxylase. Increases in RuDP carboxylase during dark growth were matched by corresponding increases in this protein zone. The correlation indicated that the enzyme accounted for 45% of the soluble protein in 11-day-old, light-grown leaves. The formation of peroxidase and catalase was much slower than the formation of RuDP carboxylase during dark growth.

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