Histochemical Studies of Lipids in Oocytes

1957 ◽  
Vol s3-98 (44) ◽  
pp. 407-424
Author(s):  
SARDUL SINGH GURAYA

Judging from their sizes and histochemical reactions, three types of lipid bodies in the form of granules and spheres have been observed in the early stages of oogenesis. The minute granules (L1), belonging to the first type appear to consist of phospholipids, triglycerides, and fatty acids, but the presence of proteins cannot be ruled out. They correspond to the so-called granular mitochondria of other authors of papers on the oogenesis of birds. The second type of lipids (L2) are of medium size and they contain phospholipids and a certain amount of triglycerides. They resemble the socalled ‘Golgi bodies’ of earlier authors in appearance and react like them with osmium tetroxide and silver nitrate. They seem to originate from the granules of the first category (L1). The third type of lipids (L3) are the AH-negative spheres consisting of triglycerides and cholesterol and its esters. They correspond to the ‘fatty yolk’ of earlier authors. Their origin appears to be from the second type of lipid bodies (L2), as intermediate bodies (int) of varying chemical composition between the L2 and L3 have been observed. The ‘peripheral lipid bodies’ (pl) found in association with vacuoles are larger than the second type of AH-positive bodies (L2), but they seem to contain different types of phospholipids, triglycerides, and some other substances also. Besides these lipid bodies, a mass of lipoprotein bodies has also been observed. At the time of their origin, the yolk globules of the advanced oocytes do not contain lipids but later on they develop triglycerides and phospholipids. The lipid bodies of the follicular cells correspond very closely to the first two categories of sudanophil AH-positive bodies (L1 and L2) of the oocyte proper.

1963 ◽  
Vol s3-104 (65) ◽  
pp. 57-68
Author(s):  
O. LUSIS

During vitellogenesis the follicular cells play an active part in the synthesis of yolk. The term ‘corpus luteum’ as applied to the ovary of the locust is invalid and two terms ‘white’ and ‘yellow’ follicle have been introduced to describe the separate identities of the normal and pathological postovulatory follicle. The protein yolk is a proteincarbohydrate compound, presumably a glycoprotein. Three kinds of lipid bodies are present; the first is a phospholipid, the second a combined phospholipid and triglyceride, and the third triglyceride. Lipids are coloured by dissolved β-carotene. Concentration or absorption of the lipids causes the crystallization of carotene and the formation of the pigment body. During resorption the follicular cells act as lecitholytic cells, first breaking down the protein and then the lipid yolk, and finally degenerating themselves. It is suggested that the oocytes have an inherent potential for resorption, the realization of which depends on various factors within the body.


2013 ◽  
Vol 54 (3) ◽  
pp. 289-299
Author(s):  
Jürgen Hunkemöller

The recognition of topoi, i.e. traditional formulae, is an important means of musical analysis. To illustrate this, the paper discusses the types of the battaglia and the pastoral in Bach’s Cantata Halt im Gedächtnis Jesum Christ, and briefly enumerates different types of allusions to jazz in 20th-century compositions by Stravinsky, Milhaud, Blacher, Tippet, and Zimmermann. Then it raises the possibility of an analysis of topoi in Bartók’s music in four main categories. It considers Bartók’s musical quotations from Bach to Shostakovich; the chorale as special topos appearing in Mikrokosmos, in the Concerto for Orchestra, in the Adagio religioso of the Third Piano Concerto; the topos-like employment of the tritone; and finally the idea of a Bartókian Arcadia in the Finale of Music for Strings, and the integration of bird song in the Adagio religioso.


Minerals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 235
Author(s):  
Nicolò Maria Ippolito ◽  
Ionela Birloaga ◽  
Francesco Ferella ◽  
Marcello Centofanti ◽  
Francesco Vegliò

The present paper is focused on the extraction of gold from high-grade e-waste, i.e., spent electronic connectors and plates, by leaching and electrowinning. These connectors are usually made up of an alloy covered by a layer of gold; sometimes, in some of them, a plastic part is also present. The applied leaching system consisted of an acid solution of diluted sulfuric acid (0.2 mol/L) with thiourea (20 g/L) as a reagent and ferric sulfate (21.8 g/L) as an oxidant. This system was applied on three different high-grade e-waste, namely: (1) Connectors with the partial gold-plated surface (Au concentration—1139 mg/kg); (2) different types of connectors with some of which with completely gold-plated surface (Au concentration—590 mg/kg); and (3) connectors and plates with the completely gold-plated surface (Au concentration—7900 mg/kg). Gold dissolution yields of 52, 94, and 49% were achieved from the first, second, and third samples, respectively. About 95% of Au recovery was achieved after 1.5 h of electrowinning at a current efficiency of only 4.06% and current consumption of 3.02 kWh/kg of Au from the leach solution of the third sample.


2021 ◽  
pp. 1-5
Author(s):  
Tayanna Bernardo Oliveira Nunes Messias ◽  
Susana Paula Alves ◽  
Rui José Branquinho Bessa ◽  
Marta Suely Madruga ◽  
Maria Teresa Bertoldo Pacheco ◽  
...  

Abstract In this research communication we describe the composition of fatty acids (FA) present in the milk of the Nordestina donkey breed, and how they differ during lactation. Milk samples were taken from 24 multiparous lactating Nordestina donkeys that grazed the Caatinga, comprising 5 animals at each of around 30, 60 and 90 d in milk (DIM) and a further 9 animals ranging from 120 to 180 DIM. The milk fat content was analysed by mid infrared spectroscopy and the FA profile by gas chromatography. The milk fat percentage ranged from 0.45 to 0.61%. The main FA found in milk were 16:0 and 18:1c9. These did not differ among DIM classes and comprised 23% and 25% of total FA. Notably, the α-Linolenic acid (18:3 n-3) was the third most abundant FA and differed (P < 0.05) with DIM, being lowest in the 30 and 60 DIM samples (around 10.7% of total FA) and highest in the 60 and 90 DIM classes (around 14.6% of total FA). The low-fat content and the FA profile of the donkey milk gives it potential as a functional ingredient, which could help to preserve the commercial viability of the Nordestina donkey breed.


1999 ◽  
Vol 276 (5) ◽  
pp. R1425-R1433 ◽  
Author(s):  
Gertjan van Dijk ◽  
Randy J. Seeley ◽  
Todd E. Thiele ◽  
Mark I. Friedman ◽  
Hong Ji ◽  
...  

To investigate whether brain leptin involves neuropeptidergic pathways influencing ingestion, metabolism, and gastrointestinal functioning, leptin (3.5 μg) was infused daily into the third cerebral ventricular of rats for 3 days. To distinguish between direct leptin effects and those secondary to leptin-induced anorexia, we studied vehicle-infused rats with food available ad libitum and those that were pair-fed to leptin-treated animals. Although body weight was comparably reduced (−8%) and plasma glycerol was comparably increased (142 and 17%, respectively) in leptin-treated and pair-fed animals relative to controls, increases in plasma fatty acids and ketones were only detected (132 and 234%, respectively) in pair-fed rats. Resting energy expenditure (−15%) and gastrointestinal fill (−50%) were reduced by pair-feeding relative to the ad libitum group, but they were not reduced by leptin treatment. Relative to controls, leptin increased hypothalamic mRNA for corticotropin-releasing hormone (CRH; 61%) and for proopiomelanocortin (POMC; 31%) but did not reduce mRNA for neuropeptide Y. These results suggest that CNS leptin prevents metabolic/gastrointestinal responses to caloric restriction by activating hypothalamic CRH- and POMC-containing pathways and raise the possibility that these peripheral responses to CNS leptin administration contribute to leptin’s anorexigenic action.


1961 ◽  
Vol s3-102 (59) ◽  
pp. 381-385
Author(s):  
SARDUL SINGH GURAYA

The cytoplasm of the human oocyte contains two categories of lipid bodies that are here called L1 and L2 for the sake of brevity. The L1 granules and spheres consist of phospholipids and triglycerides. The L2 bodies occur as aggregations of granules or fenestrated plates, consisting of phospholipids. The yolk granules are made up of phospholipids, proteins, and carbohydrates.


2013 ◽  
Vol 3 (3) ◽  
pp. 1-66
Author(s):  
Inge Pasteels ◽  
Dimitri Mortelmans

In deze bijdrage wordt het herpartneren na een scheiding uit een eerste huwelijk beschreven. Met gegevens van “Scheiding in Vlaanderen” verzameld bij 1192 mannen en 1448 vrouwen gescheiden tussen 1981 en 2005, wordt vooreerst de timing van het herpartneren alsook de leefvorm waarvoor men kiest in postmaritale relaties, bestudeerd. We onderscheiden LAT‐relaties, ongehuwd samenwoonrelaties en tweede huwelijken. Daarna wordt de huidige partnerstatus van gescheidenen anno 2010 in kaart gebracht. Ten derde komt de mate waarin partners uit eerste huwelijken of postmaritale relaties gelijkend zijn aan bod. Vervolgens wordt de stabiliteit van LAT‐relaties of ongehuwde samenwoonrelaties na een eerste huwelijk bestudeerd. Een beschrijving van postmaritale relatietrajecten met aandacht voor relatierang en relatietype rondt deze bijdrage af. Alle analyses worden apart uitgevoerd voor mannen en vrouwen van verschillende scheidingscohorten. Abstract : Using surveydata of “Divorce in Flanders” for 1192 men and 1448 women, divorced between 1981 and 2005, we explore repartnering after a first divorce. Firstly, prevalence and timing of repartnering are examined. LAT‐relationships, unmarried cohabitation and remarriage are considered as different types of repartnering. Secondly, the current partner status for divorcees in 2010 is described. Homogamy in first marriages and in higher order relationships is the third topic in this article. At last, stability of postmarital relationships and partnership trajectories are explored. Data are analyzed using a cohort perspective and a gender perspective.


2020 ◽  
Vol 29 (1) ◽  
pp. 5-24
Author(s):  
Vaskrsija Janjić

Many plants cause dermatosis in the human population, thus presenting an everyday problem for dermatologists and allergologists around the world. To this day it is still not known how common are plant-induced dermatosis. It is estimated that around 334 million people worldwide, or 3.5% of the world population, is affected by dermatosis each year. The onset of dermatitis is most common in the early childhood. Around 20% of children in Great Britain and 10% in the USA are affected by different types of dermatosis. It is believed that around 10,000 plant species can cause dermatosis in humans. Phytodermatosis are dermatosis caused by plants. They are usually induced by direct contact with a plant, but can also occur without a direct contact or be activated by the sun. Dermatosis which occur without a direct contact with the plant are observed in those cases where certain perfumes, cremes or other cosmetic products are used. Some cosmetic products contain substances such as eugenol, isoeugenol, cinnamaldehyde, colophonium, turpentines and other substances which originate from plants. Similarly, lichens, which are not plants, but organisms resulting from a fungi and algae symbiosis, produce usnic acids, which are most commonly used in sun protection products.


2020 ◽  
Vol 23 (4) ◽  
pp. 25-33
Author(s):  
Boris V. Kovaliov ◽  
◽  
Vadim E. Pugach ◽  

The article is devoted to the analysis of the functions of the zero name of proper characters in V. V. Nabokov's short prose. The factors are highlighted on the basis of which an attempt can be made to classify zero names in Nabokov's texts. The first factor is the grammatical person from whom the story is told. Particular cases related to narration from the first and third person are analyzed. The second factor is who in the text is the bearer of the zero name: the main character or the minor one? The authors pay special attention to antonomazia as a way of replacing a proper name with a null name. In the course of the study, it turns out that antonomazia can be used as a means of characterizing a character, as well as a reference not to a specific image, but to a certain class. The third factor is the possible taboo of the name as a technique. In the course of the research, two types of tabooing of a proper name are distinguished: religious and sociopsychological. The second part of the article analyzes V. V. Nabokov's story «The Razor» as an example of a text, on the basis of which one can prove the assertion about the polyfunctionality of a null name. The nomination of each character is studied in detail. The authors conclude that the opposition of full and null names plays a key role in building conflict in the plot. Moreover, the opposition of different types of nominations is a technique that determines the structure of the text, its semantic and associative levels. Based on a review of a number of Nabokov's texts and a detailed analysis of «The Razor» story, eleven functions of the zero name of V. V. Nabokov's own characters are formulated.


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