scholarly journals Reference values of 23 clinically important biochemical parameters in 107 normal pleasure horses residing in northern Greece

2018 ◽  
Vol 53 (2) ◽  
pp. 138
Author(s):  
N. DIAKAKIS (Ν.ΔΙΑΚΑΚΗΣ) ◽  
M. E. MYLONAKIS (Μ. Ε. ΜΥΛΩΝΑΚΗΣ) ◽  
N. ROUBIES (Ν. ΡΟΥΜΠΙΕΣ) ◽  
C. KOUTINAS (Χ.Κ. ΚΟΥΤΙΝΑΣ) ◽  
A. FYTIANOU (Α. ΦΥΤΙΑΝΟΥ) ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Reference Values ◽  
Biochemical Parameters ◽  
Clinical Pathology ◽  
Biochemical Parameter ◽  
Reference Interval ◽  
Biochemical Profile ◽  
Northern Greece ◽  
Clinically Normal ◽  
Abnormal Increase

The purpose of this study was το establish die reference values of die most useful biochemical parameters from a clinical ροίητ of view in pleasure horses kept in northern greek riding clubs. This biochemical profile included a total of 23 parameters such as Urtai proteins, albumins, globulins, the albumin/globulin ratio, urea nitrogen (BUN), creatinine, glucose, cholesterol, triglycerides, total and direct bilirubin, alkaline phosphatase (ALP), alanine aminotrasferase (ALT), aspartate aminotrasferase (AST), gamma-glutamylotransferase (γ-GT), creatine kinase (CK), lactate dehydrogonase (LDH), sorbitol dehydrogonase (SD), phosphorus (P), calcium (Ca), potassium (K), sodium (Na) and magnesium (Mg). A total of 107 horses, which had been considered clinically normal on historical, clinical and hematological grounds, were sampled. All the measurements were done in the blood serum, applying the relevant methods and reagents which are normally used in the Laboratory of Clinical Diagnosis and Clinical Pathology, School of Veterinary Medicine, A.U.T.. Besides the establishment of the reference interval for each biochemical parameter, all the factors that may cause an abnormal increase or decrease are discussed accordingly.

ESTABLISHING IN-HOUSE REFERENCE INTERVALS FOR DOGS IN VETERINARY CLINICS

2016 ◽  
Vol 42 (02) ◽  
pp. 53-67
Author(s):  
Shang-Hsiu Chung ◽  
Li-Wen Chang ◽  
Tsun-Li Cheng ◽  
Chen-Jou Lin ◽  
Wen-Ying Chen ◽  
...  
Keyword(s):  
Uric Acid ◽  
Bile Acid ◽  
Biochemical Parameters ◽  
Clinical Pathology ◽  
Reference Interval ◽  
Reference Intervals ◽  
Gamma Glutamyl Transferase ◽  
Coagulation Parameters ◽  
American Society ◽  
Glutamyl Transferase

Reference interval (RIs) were critical to the identification of illness. However, RIs set in one laboratory may not be appropriate for another because of biological, geographical and instrumental factors. Interpretation of clinical data using inappropriate RIs may cause misclassification of results and misdiagnosis that lead to improper treatment. RIs in Taiwan have been mostly referencing from foreign resources, it is desirable to establish one that is closer to the overall conditions in Taiwan (such as breed, climate, diseases, etc.) and to investigate its differences to foreign RIs. The present study used the American Society for Veterinary Clinical Pathology (ASVCP) guidelines to establish in-house RIs for hematological, biochemical and coagulation parameters using dogs in middle Taiwan. The results were also compared to two foreign and one local RIs. The results suggested that the hematological RIs are more comparable to foreign RIs than the biochemical and hemostatic parameters. Differences were found for biochemical parameters including gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), lipase, uric acid, bile acid, bilirubin and magnesium; and coagulation parameters including prothrombin time (PT) and activated partial thromboplastin. In all, 18% (7/40) of the all tested parameters were different from the local RI while 38% (18/48) and 41% (19/46) of the parameters were different from the two foreign RIs. The differences in more than 30% RIs and better similarities to local RIs underscore the importance of having own RIs if possible.

scholarly journals Postpartum blood biochemical profile in heifers in connection with polymorphic variants of the SCD1 gene and reproductive ability

2020 ◽  
Vol 195 (4) ◽  
pp. 57-63
Author(s):  
V. Leibova ◽  
M. Pozovnikova
Keyword(s):  
Alkaline Phosphatase ◽  
Biochemical Parameters ◽  
Leningrad Region ◽  
Biochemical Profile ◽  
Serum Samples ◽  
Holstein Breed ◽  
Blood Biochemical ◽  
Service Period ◽  
Polymorphic Variants ◽  
Glutamyl Transferase

Abstract. The purpose of this study was to compare the biochemical profile of blood in the first two months of lactation in first-calf cows with polymorphic variants of the stearoyl-CoA-desaturase gene (SCD1) and its relationship with reproductive parameters. Methods. The work was carried out in one of the breeding factories of the Leningrad Region on first-calf cows of the Holstein breed with an average milk yield of 9073 ± 263 kg for 305 days of lactation. Animal genotypes were determined by PCR-RFLP. Serum samples were used to determine the content of total protein, albumin, urea, glucose, triglycerides, cholesterol, the activity of the enzymes AST (aspartate aminotransferase), ALT (alanine aminotransferase), alkaline phosphatase (alkaline phosphatase), GGT (γ-glutamyl transferase). Laboratory studies of blood serum were performed on a RX Daytona biochemical analyzer (Randox Laboratories, UK) using reagents from Cormay, Poland. The results of the study. Of the 20 individuals, 12 animals were carriers of the CT genotype, the remaining first-calf cows had the CC (n = 6) and TT (n = 2) genotypes. Blood biochemical parameters were determined in animals with CT and SS genotypes. On the 44-50th day of lactation in animals with CT genotype, the urea concentration and AST activity decreased by 1.2–1.3 times (p ≤ 0.001). In individuals with the SS genotype, no change in these indicators was found. As a result, at the end of the second month of lactation in cows with the SS genotype, blood urea and AST activity were 1.2 times higher than in animals with the CT genotype. The content of total cholesterol increased 1.3–1.4 times (p < 0.01) by the 6–7th week of lactation in cows of both genotypes (SS and CT). The remaining biochemical parameters did not differ between time intervals within the same genotype, and between groups of animals with different genotypes. When distributing the examined cows into groups depending on the genotype and service period, it was found that the duration of the service period of more than 150 days is twice as common in individuals with the CC genotype. In cows with the heterozygous ST genotype of the SCD1 gene, biochemical profile indicators are more optimal, which may be associated with an increased frequency of occurrence of individuals with a shorter service period.

scholarly journals Hematological and biochemical profile of spider monkey (Ateles geoffroyi) in captivity

2021 ◽  
Author(s):  
Carlos David Pérez Brígido ◽  
Dora Romero Salas ◽  
Anabel Cruz Romero ◽  
Ricardo Serna Lagunes ◽  
José Luis Bravo Ramos ◽  
...  
Keyword(s):  
Reference Values ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Ateles Geoffroyi ◽  
Corpuscular Volume ◽  
Biochemical Profile ◽  
Neotropical Primates ◽  
In Captivity ◽  
Hematological And Biochemical Parameters ◽  
Normal Health

Objective: To estimate the hematological and biochemical reference values inMAteles geoffroyi individuals in captivity. Methodology: Eleven males and 23 females were captured. We collected blood,from the coccygian vein; conventional techniques were used to analyze thesamples.Results: mean corpuscular volume (MCV; P &lt; 0.03), platelets (PLT; P &lt; 0.04) were significantly higher in females than in males. In relation to biochemical blood values wasn’t significantly in male and female groups.Limitations/Implications: hematological and biochemical reference values of A. geoffroyi are within normal health parameters, are between the ranges reported for other species of neotropical primates and can be used as a reference in for the management of the health of this species in captivity.Conclusions: hematological and biochemical parameters of captive specimens of A. geoffroyi are described, which provide reference indicators for the management of the health of the species.

scholarly journals Haemato-biochemical Alterations in Obstructive and Non-obstructive Renal Diseases

2021 ◽  
Author(s):  
P. Gupta ◽  
H.R. Bhardwaj ◽  
A.K. Gupta ◽  
R.B. Kushwaha ◽  
A. Sharma ◽  
...  
Keyword(s):  
Renal Failure ◽  
Alkaline Phosphatase ◽  
Biochemical Parameters ◽  
Biochemical Parameter ◽  
Urinary Obstruction ◽  
Renal Diseases ◽  
Urinary System ◽  
Significant Morbidity ◽  
Biochemical Alterations ◽  
Healthy Dogs

Background: Diseases involving urinary system are very common in dogs causing significant morbidity and mortality in dogs. Renal failure and urinary obstruction are frequently encountered in clinical cases. Early diagnosis and treatment increase the survival rate and decreases the chances of complications related to renal failure. The present study was conducted to evaluate haemato-biochemical alterations in dogs having urinary system dysfunction. Methods: The study was conducted on 38 dogs to observe the haemato-biochemical parameter changes in dogs suffering from obstructive and non-obstructive renal system affections as compared to healthy dogs. Various haemato-biochemical parameters viz. Hb, PCV, TEC, TLC, DLC, BUN, creatinine, ALT, AST, ALP, TP, albumin and A:G ratio were evaluated. Qualitative analysis of urine was done using Multistix dip stick method. Result: In the present study the values of various haemato-biochemical parameters showed different pattern in both obstructive and non-obstructive renal diseases. Significant haematobiochemical changes reported in renal failure dogs were anemia, azotemia, increased alkaline phosphatase and AST values and decreased A:G ratio and in dogs with urinary obstruction the significant changes were uremia and leukocytosis. The results of the study suggested that the evaluation of various haemato-biochemical parameters help in diagnosis and predicting the prognosis of a renal disease.

scholarly journals Pregnancy-associated changes of serum biochemical values in Lipizzaner broodmares

2015 ◽  
Vol 63 (3) ◽  
pp. 303-316 ◽  
Author(s):  
Boglárka Vincze ◽  
Orsolya Kutasi ◽  
Ferenc Baska ◽  
Ottó Szenci
Keyword(s):  
Alkaline Phosphatase ◽  
Glutamate Dehydrogenase ◽  
Reference Values ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Clinical Chemistry ◽  
Venous Blood ◽  
Serum Triglyceride ◽  
Control Group ◽  
Aspartate Transaminase

The aims of this study were to detect physiological changes in blood biochemical parameters throughout gestation, to compare the findings in nonpregnant and pregnant Lipizzaner mares in early-mid and late pregnancy, and to provide reference values for clinical chemistry parameters in this horse breed. A total of 136 venous blood samples were collected from 20 pregnant and 10 nonpregnant (control) asymptomatic Lipizzaner broodmares for biochemical analyses. Twelve parameters (albumin, total protein, urea, triglycerides, glucose, creatinine, alkaline phosphatase, aspartate transaminase, glutamate dehydrogenase, gammaglutamyltransferase, creatine kinase and lactate dehydrogenase) were measured. For the statistical analyses, correlation, analysis of variance and Kruskal–Wallis H-test were used to evaluate the possible associations between parameters. Serum triglyceride levels proved to be significantly different in pregnant mares compared to the control group. Total protein and urea levels significantly decreased, while glucose, triglyceride and glutamate dehydrogenase values increased from approx. the fifth month of gestation until parturition. Four biochemical parameters (albumin, aspartate transaminase, total protein and urea) were lower and three other variables (glucose, alkaline phosphatase and creatinine) were significantly higher in late-term pregnant mares than in mares in early or mid-gestation. It is concluded that reference values not only reflect the species, breed and sex but also the reproductive status of animals.

Paget’s disease: clinical update

2011 ◽  
Vol 152 (33) ◽  
pp. 1337-1346
Author(s):  
Judit Donáth ◽  
Gyula Poór
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Paget’S Disease ◽  
Paget's Disease ◽  
Etiologic Role ◽  
Osteoblast Activity ◽  
Chronic Disorder ◽  
Abnormal Increase ◽  
Osteoclast Function ◽  
High Level

Paget’s disease is a chronic disorder of bone remodeling, characterized by an abnormal increase of osteoclast and, hence, osteoblast activity. The imbalance of bone turnover results in the formation of unhealthy and fragile bone. It also leads to impairment of adjacent joints and to a risk of various complications. Current research focuses on the elucidation of the etiologic role viral infection and predisposing genetic factors. Paget’s disease is commonly discovered by chance; its suspicion is raised either by high level of alkaline phosphatase or by the X-ray of the pathological bone. Bisphosphonates have proven to be effective in controlling disease activity because they inhibit osteoclast function. Their use is recommended when bone-derived serum alkaline phosphatase is high and/or when disease localizations are highly suspected for the development of complications. Orv. Hetil., 2011, 152, 1337–1346.

scholarly journals Reference values of plasma homocysteine in Cuban children and adults

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Alina Concepción Alvarez ◽  
Ivette Camayd Viera ◽  
Lisy Vento Buigues ◽  
Yanet Fernández Martínez ◽  
Eraida Hernández ◽  
...  
Keyword(s):  
Reference Values ◽  
High Performance ◽  
Age Groups ◽  
Coffee Consumption ◽  
Reference Interval ◽  
Reference Intervals ◽  
Multifactorial Diseases ◽  
Weak Association ◽  
Different Populations ◽  
Cuban Population

AbstractObjectivesHomocysteine (Hcy) is a nonessential amino acid, produced by the demethylation of methionine. High Hcy levels, or hyperhomocysteinemia, have been associated with genetic and multifactorial diseases. Hcy reference values may vary between different populations, as Hcy levels are affected by factors such as sex, age, diet, smoking, and coffee consumption. The estimation reference interval (RI) allows to establish the normal values of this marker in population. At present, these levels are unknown in Cuba. The aim of this work is to estimate the Hcy reference intervals in Cuban children and adults.MethodsTotal Hcy concentration was quantified by high performance liquid chromatography (HPLC) in plasma. Hcy levels were evaluated in samples from 507 healthy individuals (260 children, 247 adults).ResultsRIs were estimated by nonparametric methods. We found significant differences between both age groups, but we did not find significant differences between sexes, within these groups. The established ranges were 2.56–14.55 µM and 3.63–17.19 µM for children and adults, respectively. Also, we observed a weak association between Hcy levels and age in both sex groups.ConclusionsThis is the first study that assesses Hcy reference values in Cuban population. Our results will allow the introduction of Hcy as a biochemical marker in laboratory testing.

Reference values for intraocular pressure and Schirmer tear test in clinically normal Sanjabi sheep

2011 ◽  
Vol 97 (1-3) ◽  
pp. 101-103 ◽  
Author(s):  
Masoud Selk Ghaffari ◽  
Mohammad Shojaei ◽  
Amin Sabzevari ◽  
Nargess Khorami
Keyword(s):  
Intraocular Pressure ◽  
Reference Values ◽  
Clinically Normal ◽  
Schirmer Tear Test

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

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