scholarly journals Pregnancy-associated changes of serum biochemical values in Lipizzaner broodmares

2015 ◽  
Vol 63 (3) ◽  
pp. 303-316 ◽  
Author(s):  
Boglárka Vincze ◽  
Orsolya Kutasi ◽  
Ferenc Baska ◽  
Ottó Szenci
Keyword(s):  
Alkaline Phosphatase ◽  
Glutamate Dehydrogenase ◽  
Reference Values ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Clinical Chemistry ◽  
Venous Blood ◽  
Serum Triglyceride ◽  
Control Group ◽  
Aspartate Transaminase

The aims of this study were to detect physiological changes in blood biochemical parameters throughout gestation, to compare the findings in nonpregnant and pregnant Lipizzaner mares in early-mid and late pregnancy, and to provide reference values for clinical chemistry parameters in this horse breed. A total of 136 venous blood samples were collected from 20 pregnant and 10 nonpregnant (control) asymptomatic Lipizzaner broodmares for biochemical analyses. Twelve parameters (albumin, total protein, urea, triglycerides, glucose, creatinine, alkaline phosphatase, aspartate transaminase, glutamate dehydrogenase, gammaglutamyltransferase, creatine kinase and lactate dehydrogenase) were measured. For the statistical analyses, correlation, analysis of variance and Kruskal–Wallis H-test were used to evaluate the possible associations between parameters. Serum triglyceride levels proved to be significantly different in pregnant mares compared to the control group. Total protein and urea levels significantly decreased, while glucose, triglyceride and glutamate dehydrogenase values increased from approx. the fifth month of gestation until parturition. Four biochemical parameters (albumin, aspartate transaminase, total protein and urea) were lower and three other variables (glucose, alkaline phosphatase and creatinine) were significantly higher in late-term pregnant mares than in mares in early or mid-gestation. It is concluded that reference values not only reflect the species, breed and sex but also the reproductive status of animals.

scholarly journals Effects of feeding graded levels of whole cottonseed on haemato-biochemical parameters of red Sokoto bucks

2020 ◽  
Vol 18 (2) ◽  
pp. 83-90
Author(s):  
J.I. Itodo ◽  
P.I. Rekwot ◽  
T.T. Aluwong ◽  
J.S. Rwuaan ◽  
K.O. Abah ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Control Group ◽  
Transferase Activity ◽  
Albumin Concentration ◽  
Whole Cottonseed ◽  
Group D ◽  
Aspartate Transferase

Whole cottonseed (WCS) is an important source of protein for ruminants, however, it contains a polyphenolic secondary metabolite gossypol which may reduce its palatability and cause pathophysiological effects. The study was aimed at evaluating the effects of feeding graded levels of WCS on the haemato-biochemical parameters of Red Sokoto bucks (RSB). After a 14-day pre-treatment period, 20 bucks (n = 5) were assigned for 90 days to one of four isonitrogeneous treatments: control (A); 0 % (B); 15 % (C); 30 % and (D); 45 %. Blood was collected for Packed Cell Volume (PCV) and serum analyses at days 0, 45 and 90 of the experimental period for concentrations of urea, creatinine, total protein, albumin, globulin and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). The PCV was decreased in groups fed above 15 % of WCS on days 45. There were no significant (P > 0.05) differences in globulin concentration, alanine aminotransferase and alkaline phosphatase activities but aspartate transferase activity was higher (p < 0.05) in group D (45% WCS) compared to the various treatment groups. The urea concentration was higher (p < 0.05) in groups B and D at day 45 compared to the control group. The creatinine concentration was higher (p < 0.05) in group D at days 45 and 90 compared to the control group. The serum total protein and albumin concentration were higher (p < 0.05) in groups C and D at day 45 compared to the control group. Our finding revealed that prolonged feeding of bucks above 30 % WCS for more than 60 days exerted considerable deleterious and adverse effects on blood constituents leading to aneamia, hyper-cretenaemia, ureamia, increased levels of total protein, albumin, aspartate transferase activity and death. Keywords: Biochemical parameters, Bucks, Gossypol, Serum, Whole cotton seed

scholarly journals Biological characterization of implant surfaces - in vitro study

2015 ◽  
Vol 44 (4) ◽  
pp. 195-199 ◽  
Author(s):  
Priscilla Barbosa Ferreira Soares ◽  
Camilla Christian Gomes Moura ◽  
Huberth Alexandre da Rocha Júnior ◽  
Paula Dechichi ◽  
Darceny Zanetta-Barbosa
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Viability ◽  
Total Protein ◽  
Cell Attachment ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Serum Total Protein ◽  
Trypan Blue Exclusion ◽  
Experimental Surface

<title>Abstract</title><sec><title>Objective</title><p>Evaluate the biological performance of titanium alloys grade IV under different surface treatments: sandblasting and double etching (Experimental surface 1; Exp1, NEODENT); surface with wettability increase (Experimental surface 2; Exp2, NEODENT) on response of preliminary differentiation and cell maturation.</p></sec><sec><title>Material and method</title><p>Immortalized osteoblast cells were plated on Exp1 and Exp2 titanium discs. The polystyrene plate surface without disc was used as control group (C). Cell viability was assessed by measuring mitochondrial activity (MTT) at 4 and 24 h (n = 5), cell attachment was performed using trypan blue exclusion within 4 hours (n = 5), serum total protein and alkaline phosphatase normalization was performed at 4, 7 and 14 days (n = 5). Data were analyzed using one-way ANOVA and Tukey test.</p></sec><sec><title>Result</title><p>The values of cell viability were: 4h: C– 0.32±0.01<sup>A</sup>; Exp1– 0.34±0.08<sup>A</sup>; Exp2– 0.29±0.03<sup>A</sup>. 24h: C– 0.43±0.02<sup>A</sup>; Exp1– 0.39±0.01<sup>A</sup>; Exp2– 0.37±0.03<sup>A</sup>. The cell adhesion counting was: C– 85±10<sup>A</sup>; Exp1- 35±5<sup>B</sup>; Exp2– 20±2<sup>B</sup>. The amounts of serum total protein were 4d: C– 40±2<sup>B</sup>; Exp1– 120±10<sup>A</sup>; Exp2– 130±20<sup>A</sup>. 7d: C– 38±2<sup>B</sup>; Exp1– 75±4<sup>A</sup>; Exp2– 70±6<sup>A</sup>. 14 d: C– 100±3<sup>A</sup>; Exp1– 130±5<sup>A</sup>; Exp2– 137±9<sup>A</sup>. The values of alkaline phosphatase normalization were: 4d: C– 2.0±0.1<sup>C</sup>; Exp1– 5.1±0.8<sup>B</sup>; Exp2– 9.8±2.0<sup>A</sup>. 7d: C– 1.0±0.01<sup>C</sup>; Exp1– 5.3±0.5<sup>A</sup>; Exp2– 3.0±0.3<sup>B</sup>. 14 d: C– 4.1±0.3<sup>A</sup>; Exp1– 4.4±0.8<sup>A</sup>; Exp2– 2.2±0.2<sup>B</sup>. Different letters related to statistical differences.</p></sec><sec><title>Conclusion</title><p>The surfaces tested exhibit different behavior at dosage of alkaline phosphatase normalization showing that the Exp2 is more associated with induction of cell differentiation process and that Exp1 is more related to the mineralization process.</p></sec>

scholarly journals Reference values of 23 clinically important biochemical parameters in 107 normal pleasure horses residing in northern Greece

2018 ◽  
Vol 53 (2) ◽  
pp. 138
Author(s):  
N. DIAKAKIS (Ν.ΔΙΑΚΑΚΗΣ) ◽  
M. E. MYLONAKIS (Μ. Ε. ΜΥΛΩΝΑΚΗΣ) ◽  
N. ROUBIES (Ν. ΡΟΥΜΠΙΕΣ) ◽  
C. KOUTINAS (Χ.Κ. ΚΟΥΤΙΝΑΣ) ◽  
A. FYTIANOU (Α. ΦΥΤΙΑΝΟΥ) ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Reference Values ◽  
Biochemical Parameters ◽  
Clinical Pathology ◽  
Biochemical Parameter ◽  
Reference Interval ◽  
Biochemical Profile ◽  
Northern Greece ◽  
Clinically Normal ◽  
Abnormal Increase

The purpose of this study was το establish die reference values of die most useful biochemical parameters from a clinical ροίητ of view in pleasure horses kept in northern greek riding clubs. This biochemical profile included a total of 23 parameters such as Urtai proteins, albumins, globulins, the albumin/globulin ratio, urea nitrogen (BUN), creatinine, glucose, cholesterol, triglycerides, total and direct bilirubin, alkaline phosphatase (ALP), alanine aminotrasferase (ALT), aspartate aminotrasferase (AST), gamma-glutamylotransferase (γ-GT), creatine kinase (CK), lactate dehydrogonase (LDH), sorbitol dehydrogonase (SD), phosphorus (P), calcium (Ca), potassium (K), sodium (Na) and magnesium (Mg). A total of 107 horses, which had been considered clinically normal on historical, clinical and hematological grounds, were sampled. All the measurements were done in the blood serum, applying the relevant methods and reagents which are normally used in the Laboratory of Clinical Diagnosis and Clinical Pathology, School of Veterinary Medicine, A.U.T.. Besides the establishment of the reference interval for each biochemical parameter, all the factors that may cause an abnormal increase or decrease are discussed accordingly.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

Reference intervals for 21 clinical chemistry analytes in arterial and venous umbilical cord blood

1993 ◽  
Vol 39 (6) ◽  
pp. 1041-1044 ◽  
Author(s):  
S L Perkins ◽  
J F Livesey ◽  
J Belcher
Keyword(s):  
Alkaline Phosphatase ◽  
Umbilical Cord ◽  
Clinical Chemistry ◽  
Venous Blood ◽  
Reference Interval ◽  
Reference Intervals ◽  
Nonparametric Analysis ◽  
Term Infants ◽  
Male And Female ◽  
Gamma Glutamyltransferase

Abstract Reference intervals were determined for 21 clinical chemistry analytes in umbilical cord arterial and venous blood from healthy term infants. Nonparametric analysis (rank number) was used to determine the central 95% reference interval. No significant differences were observed between male and female infants. Reference intervals for glucose, urea, creatinine, urate, phosphate, calcium, albumin, total protein, cholesterol, triglycerides, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, creatine kinase, lactate dehydrogenase, gamma-glutamyltransferase, and magnesium all were significantly different from adult values.

scholarly journals Plasma Calcium Levels in Pregnant Asian Women

1976 ◽  
Vol 13 (1-6) ◽  
pp. 339-344 ◽  
Author(s):  
Nina Polanska ◽  
R. A. Dale ◽  
M. R. Wills
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Dietary Intake ◽  
Total Protein ◽  
Plasma Calcium ◽  
Asian Women ◽  
Control Group ◽  
Calcium Phosphorus ◽  
And Control ◽  
Endogenous Synthesis

Plasma calcium, phosphorus, alkaline phosphatase, total protein, and albumin were measured during pregnancy in a group of Asian women living in the south of England and in a control group. The Asian women had slightly lower mean plasma calcium concentrations than the control group. Exposure of Asian women to sunshine appeared to be low, and it was inferred that they probably had little endogenous synthesis of cholecalciferol as a result. Both Asian and control subjects ingested similarly low amounts of vitamin D. The Asian women consumed greater amounts of phytate and vegetable fibres than the controls, and it is suggested that these substances may reduce absorption of insoluble lipids (including cholecalciferol) and calcium in a setting where the dietary intake and endogenous synthesis of vitamin D are already borderline for the subjects' requirements.

scholarly journals Toxic Effect of Ladosulfan-Pesticides on Biochemical Indices of Clarias Gariepinus (Burchell 1822) Juveniles

2020 ◽  
Vol 3 (3) ◽  
Keyword(s):  
Water Quality ◽  
Alkaline Phosphatase ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Clarias Gariepinus ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Commercial Formulation ◽  
Aspartate Amino Transferase ◽  
Biochemical Indices

ladosufan is an insecticide extensively used in agriculture for pest control. The 96h LC50 estimated from probeit analysis was 1.89mg/L. Sub lethal concentrations of 1/10th (0.19 mg/L),1/5th (0.49mg/L) and 1/2th (0.95mg/L) of LC50 were used to determine the effects of the ladosulfan -pesticide on biochemical parameters at day 1, 7, 14 and 21exposure periods. Water quality test was determined on pH, temperature, dissolved oxygen, hardness and alkalinity. The results show that biochemical parameters viz; Total Protein, Alkaline Phosphatase ALP, Alanine Transferase ALTAspartate Amino Transferase (AST) concentrations increased significantly (P ≤ 0.05) in time and concentration dependent manner. The biochemical parameters concentration had higher values in treated samples than control. The total protein increased from (8.80±0.002b -9.20±0.103b) at 0.19 mg/L, (9.20±0.102a –9.70±0.103c) at 0.48 mg/L and (9.27±0.152a –9.80±0.103c) at 0.95 mg/L of ladosulfan when compared with the control (7.67±0.931c –9.06±0.763c ); Alkaline Phosphatase increased from (60.00±0.002a -86.00±1.001b) at 0.19 mg/L, (62.00±1.002b –88.00±1.001c) at 0.48 mg/L and (65.00±1.001a –92.00±1.002d) at 0.95 mg/L of endosulfan when compared with the control (40.00±1.002a–53.67±5.511a) ; Alanine Transferase also increased from (30.00±1.002a -40.00±1.001c) at 0.19 mg/L, (35.00±1.002a –54.00±1.001a) at 0.48 mg/L and (38.00±1.002b –89.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (22.33±2.083c –27.33±2.882b) ; Aspartate Amino Transferase increased from (64.00±1.002b -87.00±1.001c) at 0.19 mg/L, (66.00±1.002c –88.00±2.001d) at 0.48 mg/L and (68.33±0.582c –90.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (63.00±2.002b –68.00±1.001b) ; respectively. The results indicate that commercial formulation of ladosulfan is toxic to C. gariepinus, the insecticide should be prudently used in both terrestrial and aquatic eco-systems to avoid eco-toxicological hazards.

scholarly journals The effect of camel milk curd masses on rats blood serum biochemical parameters: Preliminary study

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256661
Author(s):  
Fatima Dikhanbayeva ◽  
Elmira Zhaxybayeva ◽  
Zhuldyz Smailova ◽  
Arman Issimov ◽  
Zhechko Dimitrov ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Camel Milk ◽  
Control Group ◽  
Serum Biochemical Parameters ◽  
Group V ◽  
Group I ◽  
Serum Biochemical ◽  
Group Ii

This study aimed to assess potential feeding effect of camel milk curd mass and its mixes to experimental rat’s blood serum biochemical parameters, enzymatic activity and the peptide toxicity. Fifty healthy male Sprague-Dawley rats were divided into five groups (n = 10 each). Each group was fed with camel milk pure curd mass and its mixes for 16 days. At the end of the experiment, rats were sacrificed to collect the samples from the blood serum. Blood serum biochemical parameters total protein, cholesterol, glucose, albumin, triglycerides; the enzymatic activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were determined on the A25 automatic analyser, and peptide toxicity analysed by the reference method. The statistical data have shown no significant differences in body weight gain in all groups. Total protein decreased in group II, IV, and V; however, it increased in group III compared to the control group. Cholesterol grew up in group II and it slightly increased in group V, dropped in groups III and IV compared to group I result. Glucose increased in groups II, III, IV compared to group I; still, group V results show a slight decrease. Albumin decreased in group IV, yet in group V it increased than the group I result. Simultaneously, groups II and III results were changed with less percentage. Triglyceride grew up in groups II, V, and it dropped significantly in groups III, IV compared to the control group. De Ritis ratio of enzymes in groups II, III, and IV fluctuated between 1.31 and 0.98 IU/L; however, group V demonstrated significant data versus group I. Diets peptide toxicity in all groups was lower than control group data. The experimental results indicated that curd mass from camel milk could be used as a pure or with additives and it did not discover the observed side effects.

scholarly journals Comparison of the effects of basil (<i>Ocimum basilicum</i>) essential oil, avilamycin and protexin on broiler performance, blood biochemistry and carcass characteristics

2015 ◽  
Vol 58 (2) ◽  
pp. 425-432 ◽  
Author(s):  
S. R. Riyazi ◽  
Y. Ebrahimnezhad ◽  
S. A. Hosseini ◽  
A. Meimandipour ◽  
A. Ghorbani
Keyword(s):  
Essential Oil ◽  
Biochemical Parameters ◽  
Body Weight Gain ◽  
Density Lipoprotein ◽  
Serum Triglyceride ◽  
Carcass Characteristics ◽  
Feed Additives ◽  
Control Group ◽  
Growth Promoter ◽  
Feed Conversion

Abstract. The effects of a probiotic (protexin), a medicinal plant (basil essential oil) and an antibiotic growth promoter (avilamycin) as broiler feed additives on performance, carcass characteristics and some blood parameters of broilers were studied. A total of 600 Arian broilers were divided into six treatments, with four replicates of 25 birds. Treatments were a plant essential oil in three levels (200, 400 and 600 ppm), the probiotic (150 ppm), the antibiotic (150 ppm) and a control group with no additives. Birds in different treatments received the same diets during the experimental period. Growth performance, blood biochemical parameters and carcass traits were measured. There were no effects of dietary treatment on body weight gain, feed intake and feed conversion ratio of the broilers. Internal organ weights and carcass characteristics were not influenced by treatments; however, 200 ppm basil essential oil supplementation decreased abdominal fat (P < 0.05). Biochemical parameters including serum low-density lipoprotein (LDL), high-density lipoprotein (HDL) and total cholesterol were not statistically influenced. However, addition of 400 ppm basil essential oil into diet increased serum triglyceride as compared to the control (P < 0.05). Results of this experiment demonstrated that the herbal natural feed additives such as basil may be used as alternatives to an antibiotic growth promoter without any adverse effects on broiler production.

249 BIOPHYSICAL AND BIOCHEMICAL CHARACTERISTICS OF ALPACA SEMEN AFTER COLLECTION BY ELECTROEJACULATION

2013 ◽  
Vol 25 (1) ◽  
pp. 272 ◽  
Author(s):  
J. Rodriguez ◽  
W. Huanca ◽  
M. Ramos ◽  
M. Vasquez ◽  
J. Espinoza
Keyword(s):  
Alkaline Phosphatase ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Alanine Transaminase ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Biochemical Characteristics

The collection of alpaca semen presents difficulties because of the characteristics of mounts and copulation time in addition to the high viscosity of semen. The biophysical and biochemical characteristics of alpaca semen were investigated. Semen samples from four adult males from 6 to 7 years of age were collected using an electroejaculation technique. The biophysical parameters measured in seminal plasma included volume, color, sperm concentration, motility, and viscosity, and the biochemical parameters included pH and glucose, calcium, triglyceride, cholesterol, high-density lipoprotein cholesterol, total protein, albumin, alanine transaminase, alkaline phosphatase, and γ-glutamyltranspeptidase concentrations. The mean time for semen collection was 10.3 ± 1.5 min; the volume varied from 1.2 to 3.8 and the colour from clear to milky, and pH was 7.4 ± 0.03. Mean concentration of spermatozoa was 69.3 ± 193 million mL–1, and motility was 52.4 ± 9.7%. Biochemical parameters were glucose (mg dL–1) 8.22 ± 0.77; cholesterol (mg dL–1) 79.78 ± 5.64; triglycerides (mg dL–1) 44.12 ± 7.38; high-density lipoprotein cholesterol (mg dL–1) 4.73 ± 0.30; total protein (g dL–1) 2.36 ± 0.15; albumin (g dL–1) 0.97 ± 0.33; calcium (mg dL–1) 11.77 ± 1.74; alanine transaminase (U L–1) 17.92 ± 9.09; alkaline phosphatase (U L–1) 288.76 ± 279.59; and γ-glutamyltranspeptidase (U L–1) 89.62 ± 39.09. These results provide information on the biophysical and biochemical characteristics of alpaca semen collected by electroejaculation, with variable results in the enzymatic components.

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