Effect of Fibrinolytic Microbes and Enzymes on Biochemical Blood Parameters in Crossbred Calves

2019 ◽  
Vol 15 (02) ◽  
pp. 18-21
Author(s):  
R I Kapadiya ◽  
S V Shah ◽  
Y G Patel ◽  
P R Pandya
Keyword(s):  
Blood Parameters ◽  
The Other ◽  
Control Group ◽  
Glutamic Pyruvic Transaminase ◽  
Normal Range ◽  
Blood Samples ◽  
Overall Performance ◽  
Similar Body ◽  
Control Plasma ◽  
Plasma Phosphorus

Twenty crossbred (HF x Kankrej) calves were allotted randomly to five groups (each had one male and three female calves) almost with similar body weight (85.70±6.37 kg) and age (167.55±21.70 days). Experimental calves of the control group were offered hybrid napier untreated, and the other four groups were offered hybrid napier treated with (i) fungus- Aspergillus spp. (1×107 per g feed), (ii) fibrolytic bacteria- Escherichia spp. (106 CFU per g feed), (iii) xylanase (50 mL/kg having xylanase 1.2 IU/mL), and (iv) consortium of 1/3rd dose of fungus + bacteria + enzyme as treatments for 140 days. The blood samples were collected from each calf at a biweekly intervals in the EDTA vacuttee. Nutrients offered to crossbred calves were sufficient to satisfy the nutrients requirements. Bacteria fed calves had significantly higher (plessthan0.05) mean hemoglobin levels than control. Blood plasma glucose of calves in fibrolytic microbes and enzyme groups was statistically similar to control. Feeding of fungus, bacteria, enzyme, and consortium was without a significant effect on plasma total proteins.In comparison to control (7.53 mg/dL), the plasma phosphorus was significantly (plessthan0 0.05) higher in calves fed enzyme (7.87 ± 0.20 mg/dl) and lower in group fed fungus (7.13 mg/dL). In contrast, in bacteria (7.57 mg/dL) and consortium group (7.60 mg/dL) it was statistically similar to control. Plasma glutamic pyruvic transaminase concentration of crossbred calves was lower ( pless than 0.05) in enzyme and higher (p lessthan 0.05) in fungus, bacteria, and consortium groups than in control, while plasma glutamic oxaloacetate transaminase concentration in all groups was statistically similar. In conclusion, all blood parameters of different groups were within normal range and without adverse effect on the overall performance of calves.

scholarly journals Effect of different doses of uranyl acetate on some blood parameters and hepatic enzymesin females rats.

2014 ◽  
Vol 8 (3) ◽  
pp. 37-43
Author(s):  
Sarah Ghazi Alzorii
Keyword(s):  
Oral Administration ◽  
Liver Enzyme ◽  
Blood Parameters ◽  
Uranyl Acetate ◽  
The Other ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Different Doses

This study was carried out to investigate effects of oral administration of uranyl acetate on haematological parameters and liver enzyme. For this purpose, twenty females mature Albino rats were divided into four equal groups, control group G1 administrated distilled water, the other three groups administrated orally 50,75,100 mg/kg/b.w. /day of uranyl acetate. The route of administration was oral intubations for 10 weeks, for 14 days before mating with untreated males, as well as during pregnancy 3 weeks and lactation 5 weeks. At the end of the treatment blood samples were collected from mothers of rats. The results were obtained a significant P< 0.05 increase in the total WBC counts in groups G2,G3,G4 compared with control .A significant P<0.05 decrease in the lymphocytes and monocytes percentage in groups (G3,G4), while no significant decrease P>0.05 in G2 in the lymphocytes and monocytes percentage compared with control. And the results show significant P< 0.05 decrease in HB value in groups G3,G4, while no significant decrease P>0.05 in G2 in HB value compared with control. A significant P< 0.05 decrease was observed in total RBCs counts in groups G2,G3,G4 compared with control. No significant increase P>0.05 in GOT and GPT in groups G2 and G3 and significant P<0.05 increase in GOT and GPT activity in the serum in group G4 compared with control. No significant increase P>0.05 in CPK activity in groups G2,G3,G4 compared with control.

scholarly journals Serum Protein Profile Of Iraqi Hydatidosis Patients with Different Sites of Infection

2007 ◽  
Vol 4 (4) ◽  
pp. 612-616
Author(s):  
Baghdad Science Journal
Keyword(s):  
Serum Protein ◽  
Protein Profile ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Healthy Individuals ◽  
Banding Patterns ◽  
Blood Samples ◽  
Molecular Weights ◽  
Serum Protein Profile

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

scholarly journals 380 Effects of estradiol benzoate on reproductive characteristics in beef cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 145-145
Author(s):  
Lacey M Dysart ◽  
Caleb O Lemley ◽  
Riley Messman ◽  
Jamie E Larson
Keyword(s):  
Doppler Ultrasonography ◽  
Blood Perfusion ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
The Other ◽  
Control Group ◽  
Blood Samples ◽  
Doppler Probe ◽  
Imagej Software

Abstract Ovulation prompted by exogenous GnRH may cause the release of sub-mature oocytes and lead to decreased pregnancy rates. Inclusion of estradiol benzoate (EB) has improved pregnancy rates. The objective was to determine if EB affects blood perfusion of follicles, CL, or uterus, concentrations of estradiol, or incidence of standing estrus. Forty-seven suckled beef cows were synchronized (GnRH+CIDR on d -9, PGF2a+CIDR removal on d -2, expected estrus=d 0). On d -1, 24 of 47 cows received an injection of EB (1mg/2mL) while the other 23 remained as a control group. Ovaries were evaluated via Doppler ultrasonography on d -1, 0, 6, 14, and 21. A laser Doppler probe was used to measure blood perfusion of the uterus on d -1, 0, and 6. Only cows that exhibited estrus (n = 29) were evaluated on d 14 and 21. Images were analyzed via ImageJ software to determine mean area of perfusion. Blood samples were collected on d -1 and 0 and analyzed for concentrations of estradiol using RIA. Using SAS, MIXED and FREQ were used to determine the influence of treatment on blood perfusion and estradiol, and incidence of standing estrus, respectively. Treatment with EB increased (P &lt; 0.001) the number of cows exhibiting standing estrus compared to control (21/24 vs 8/23, respectively). Treatment of EB did not (P &gt; 0.10) alter blood perfusion of the follicles or uterus on any day or perfusion of the CL on d 6. However, EB increased (P &lt; 0.036) the size of the CL on d 14 (25.06±1.7 vs 6.06±3.7) and tended (P &lt; 0.097) to increase the size of the CL on d 21. Treatment of EB increased (P &lt; 0.003) concentrations of estradiol on d 0 compared to control (21.35±4.49 vs 9.44±4.61 pg/mL). In conclusion, treatment with EB may play a role in changing the CL after ovulation.

scholarly journals A Propensity Score Cohort Study on the Long-Term Safety and Efficacy of Sleeve Gastrectomy in Patients Older Than Age 60

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Judith Molero ◽  
Romina Olbeyra ◽  
Josep Vidal ◽  
Ferran Torres ◽  
Silvia Cañizares ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Control Group ◽  
Normal Range ◽  
Inverse Probability ◽  
The Past ◽  
Obese Subjects ◽  
Similar Body

Background. Bariatric surgery (BS) in older obese subjects (>60 years of age) has risen in the past decade and will continue to rise in the coming years due to ageing of the population. Aim. To evaluate the short- (12 months) and long-term (60 months) results of laparoscopic sleeve gastroscopy (LSG) in patients older than age 60. Methods. We performed a retrospective review of patients prospectively included in a database from January 2007 to December 2013. All patients >60 [older group (OG)] who had undergone LSG were included. The control group (CG) included patients aged 50 to 59 years who had undergone LSG during the same period. Results. 116 (8.4 % of total surgery) and 145 patients were included in the OG and CG, respectively. BS in patients >60 years increased from 2.4% in 2003 to 14% in the last 2 years of the study. After inverse probability of treatment weighting (IPTW) analysis, all absolute standardized differences were <0.15. A 60-month follow-up was attained in 90% of patients in the OG and 74% in the CG. There were no significant differences in postoperative complications between the two groups. At 12 and 60 months after LSG, both groups achieved a similar body mass index. There was no statistical difference in the percentage of resolution of type 2 diabetes, hypertension, dyslipidemia, and SAHS between the two groups. In both groups, all the nutritional parameters evaluated remained within the normal range throughout the study. Conclusions. LSG provides acceptable outcomes and is safe in older adults indicating that age should not be a limitation to perform BS in this population.

scholarly journals The Importance of Holotranscobalamin Measurement

2007 ◽  
Vol 26 (3) ◽  
pp. 227-230 ◽  
Author(s):  
Velibor Čabarkapa ◽  
Zoran Stošić ◽  
Radmila Žeravica ◽  
Branislava Ilinčić ◽  
Ana Filipović
Keyword(s):  
Significant Relationship ◽  
Total Serum ◽  
Control Group ◽  
Vitamin B ◽  
Normal Range ◽  
Total Vitamin ◽  
Blood Samples ◽  
Low Levels ◽  
Clinically Significant ◽  
M 14

The Importance of Holotranscobalamin MeasurementClinically significant vitamin B12deficiency can occur even with total vitamin B12levels apparently within normal range. There is an indeterminate zone between approximately 154 and 300 pmol/L of vitamin B12where there is likely misclassification of B12status if relying on total serum B12. The aim of the present study was to assess the usefulness of holotranscobalamin in diagnosis of B12deficiency. Blood samples were collected and subjected to assays for vitamin B12and holotranscobalamin. We examined the levels of holotranscobalamin in 32 subjects (n=32, f=18, m=14) with vitamin B12values within interval 154-300 pmol/L. These subjects were compared with control group with vitamin B12>300 pmol/L (n=31, f=17, m=14). 25% of subjects with vitamin B12of 154 to 300 pmol/L had low levels of holotranscobalamin. Holotranscobalamin levels of patients with vitamin B12of 154 to 300 pmol/L were significantly lower than those of control subjects (38.55 ± 23.0 vs. 61.35 ± 31.81 pmol/L, p<0.01). The obtained results also show a positive significant relationship between levels of holotranscobalamin and vitamin B12(r=0.53, p<0.01). In conclusion, holotranscobalamin is a better indicator of early vitamin B12deficiency than total serum cobalamins and it is a useful tool in the diagnosis of vitamin B12deficiency.

scholarly journals Effects of Feeding Diets Containing Pearl Millet Supplemented with Enzyme on the Carcass and Haematological Values of Broiler Chicken

2021 ◽  
pp. 52-60
Author(s):  
John Amedu Edache ◽  
Lydia Inyam ◽  
Stephen Ejembi Edache
Keyword(s):  
Pearl Millet ◽  
Broiler Chickens ◽  
Energy Content ◽  
Control Diet ◽  
Blood Parameters ◽  
Metabolizable Energy ◽  
The Other ◽  
Control Group ◽  
Haematological Values ◽  
Feeding Diets

Pearl millet, which is comparable to maize in terms of its metabolizable energy content is an identified potential replacement for maize in broiler diets. However, as the competition and price of maize continues to spike, the continuous need to completely replace maize in broiler diets is imperative. Therefore, the objectives of this study were to investigate the effects of feeding diets containing pearl millet supplemented with enzyme NatuzymeTM on the carcass characteristics and haematological values of broiler chickens. 150 two weeks-old broilers were randomly divided into 5 groups with 10 birds per pen translating into 10 birds per replicate and fed an isonitrogenous (23%CP) starter diet of control diet (A); 0% (B); 10% (C); 20% (D); 30.01% and (E) 37.41 % pearl millet. Similarly, an isonitrogenous (21%CP) finisher diet of control diet (A); 0% (B); 10.64% pearl millet (C); 21.28% (D); 31.92% and (E) 39.27 % of pearl millet for 6 weeks. The birds were randomly allotted to the diets in a completely randomized design with feed and water given ad libitum. With the exception of drumstick of birds fed diet D which was significantly lower than the other diets, the results showed that the carcass values (parameters) of broilers fed 100% pearl millet were similar or non-superior to the control group and the other diets. Similarly, blood parameters were not significantly different between groups. These results show that pearl millet supplemented with enzyme can replace maize in broiler diets without affecting carcass yields and haematological parameters.

scholarly journals The Changes of Whole Blood Cells and Plasma Proteins in Donors After Plateletpheresis: 42 Donors With a Interval of 14-16 Days and More Than 20 Times

2021 ◽  
Author(s):  
Jun Li ◽  
Hongmei Liao ◽  
Danfeng Cheng
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Venous Blood ◽  
Control Group ◽  
Observation Group ◽  
Research Subjects ◽  
Normal Range ◽  
Blood Samples ◽  
Whole Blood Cells

Abstract Background To study the changes of whole blood cells and plasma proteins in donors after plateletpheresis with multiple donations. Materials and Methods From October 2015 to September 2019, 42 donors with a plateletpheresis interval of 14-16 days and more than 20 times were selected as the research subjects. The venous blood samples were collected from the first and the last screening before plateletpheresis. The result of last screening before plateletpheresis as the observation group, and the first as the control group. Then, the venous blood samples was detected. Results The whole blood cells and plasma proteins in donors after plateletpheresis changes within a normal range in the two groups. The PLT counts in the the observation group was 220.1±40.4 x109/L, which was no statistically significant compared with the change of 216.6±44.5 x109/L in the control group(P>0.05). The HGB in the the observation group was 142.8±10.2 g/L, which was no statistically significant compared with the change of 142.1±8.3g/L in the control group(P>0.05). The HCT in the the observation group was 43.50±3.2%, which was no statistically significant compared with the change of 44.1±2.8% in the control group(P>0.05). The serum TP levels in the the observation group was 70.4±4.7g/L, which was no statistically significant compared with the change of 69.0±4.8g/L in the control group(P>0.05). The serum ALB levels in the observation group was 46.3±2.3g/L, which was no statistically significant compared with the change of 45.8±2.3g/L in the control group(P>0.05). Conclusion There have no effect on the whole blood cells and plasma proteins in donors after plateletpheresis with multiple donations.

Effect of Tylvalosin on Blood Parameters in Healthy Broiler Chickens

2021 ◽  
Vol 19 (8) ◽  
pp. 141-144
Author(s):  
Mohammed Mosleh Shwaish ◽  
Ammar H. Salman ◽  
Asif Hasan Abdulrazaq
Keyword(s):  
Body Weight ◽  
Normal Saline ◽  
Broiler Chickens ◽  
Treated Group ◽  
Blood Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Blood Samples

To evaluate the effects of Tylvalosin on blood parameters in healthy broiler chickens, twenty broiler chicks (Ross 308) aged 21 days old and weight 600-800 gm was divided into two equal groups, the first one was control group (G1) and the second one was treated group (G2). G1 dosed with 1ml/kg of body weight of normal saline while G2 dosed with 25mg/Kg of body weight of Tylvalosin. Blood samples were collected with EDTA tubes after 4 days of treatment. The results showed significant decrease in total WBCs counts in G2 treated group as compared with control group (G1), also the eosinophil showed a significant decrease in G2 as compared with G1, while other parameters didn’t show any significant differences. In conclusion, WBCs were decreased after treatment with tylvalosin.

scholarly journals Effect of Varying Doses of Estradiol Benzoate on the body weight and blood picture in Turkeys

2012 ◽  
Vol 36 (2) ◽  
pp. 174-179
Author(s):  
Saad J. Gatie, Bassam A. Al-Shimmary
Keyword(s):  
Body Weight ◽  
Estradiol Benzoate ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Blood Picture ◽  
Blood Samples ◽  
The Third ◽  
Control Body ◽  
Rbc Count

A present study was carried out to investigate the effects of Estradiol benzoate 2 (mg/ml) hormone doses on the body weight and blood picture of Turkeys birds through studying the following parameters: body weight, RBC count , total WBC, MCV, MCH, MCHC, and platelet PLT. Forty growing Turkeys (30 days age) weighting between (250-350)gm were randomly divided into four groups (ten each group) treated as follows: Turkeys in the first group were injected with single dose of Estradiol benzoate (1 mg) on the first day of experiment (30 day post hatching) subcutaneously in the neck and termed as G1 group, the Turkeys in the 2nd group (G2) were injected Estradiol benzoate (2 mg) on the first day of experiment subcutaneously in the neck while the Turkeys in the third group (G3) were injected Estradiol benzoate (0.3 ml) at the first day of experiment subcutaneously in the neck the fourth group (G4) served as control, body weight was determined on (0, 15 and 30) days of experiment and blood samples were collected on 15th and 30th day of the experiment from groups to evaluate the blood parameters. Results revealed a significant increase (P<0.05) in body weight of G1, G2 and G3 in a comparison with control group at (15 and 30) days of experiment. Furthermore, in comparison among treated groups G2 and G3 also showed a significant increase (P<0.05) in the body weight as compared with G1 group during periods. Moreover, data revealed that all treated groups showed non-significant differences in blood parameters experiment as compared with control group.

scholarly journals Growth Performance, Nutrient Digestibility, Biochemical Properties, Hematological Traits, and Intestinal Histopathology of Broiler Chicks Fed Mannan Oligosaccharides

2021 ◽  
Vol 11 (4) ◽  
pp. 621-633
Author(s):  
Abdallah Ali Ghazalah ◽  
Mohamed Ahmed Fouad El-Manylawi ◽  
Hady Fathy Abbas Motawe ◽  
Marwa Salah Khattab ◽  
Yara Ibrahem Youssef
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Blood Parameters ◽  
Nutrient Digestibility ◽  
The Other ◽  
Control Group ◽  
Broiler Chicks ◽  
Treatment Groups

, , , , and Antibiotics as growth promoters in poultry diets are currently restricted, so other feed additives, such as prebiotics and probiotics, have been suggested as an antibiotics alternative to improve the performance and gut health of poultry. The current experiment was conducted to study the effects of adding Mannan oligosaccharides (MOS) as a potential replacement for an antibiotic on productive performance, nutrient digestibility, some blood parameters, and caecal microbiota of broiler chickens. For conducting the current research experiment, a total of 180 one–day old Ross broiler chicks were randomly divided into one control group fed a basal diet and four dietary treatments with six replicates for each treatment. The treatment groups were fed a basal diet supplemented with different levels of MOS 0.5, 1, and 2 g/Kg diet and Lincomycin 4.4 mg/Kg diet during 35 days of the feeding trial. With exception of the growing period, the group fed a basal diet supplemented with 2 g MOS/Kg feed had considerably higher body weight and weight gain, while having the lowest feed consumption and best feed conversion ratio compared to the other treatment groups, during all experimental periods. Moreover, dietary supplementation of MOS resulted in a significant decrease in the counts of caecal E. coli and Enterococcus, while Lactobacillus and Yeast bacteria counts were significantly higher, compared to non-supplemented groups. Broiler chicks having a 2 g MOS/kg diet recorded lower values of cholesterol, low-density lipoprotein (LDL), urea, and liver enzymes, including Aspartate transaminase (AST) and Alanine aminotransferase (ALT), while they recorded significantly higher high-density lipoprotein (HDL), compared to the other experimental groups. Group treated with MOS 2 g/Kg feed improved digestibility of crude protein, ether extract, crude fiber, nitrogen-free extract, and organic matter, compared to the control group, Additionally, MOS supplementation also increased the intestinal villi length, width, and crypt depth and decreased intestinal inflammation, compared to the control group. In conclusion, supplementation of MOS at 2 g/kg diet improved growth performance, digestibility, and blood parameters without having adverse effects on the intestine of broiler chickens, comparable to the Lincomycin.

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