Effect of different doses of uranyl acetate on some blood parameters and hepatic enzymesin females rats.

This study was carried out to investigate effects of oral administration of uranyl acetate on haematological parameters and liver enzyme. For this purpose, twenty females mature Albino rats were divided into four equal groups, control group G1 administrated distilled water, the other three groups administrated orally 50,75,100 mg/kg/b.w. /day of uranyl acetate. The route of administration was oral intubations for 10 weeks, for 14 days before mating with untreated males, as well as during pregnancy 3 weeks and lactation 5 weeks. At the end of the treatment blood samples were collected from mothers of rats. The results were obtained a significant P< 0.05 increase in the total WBC counts in groups G2,G3,G4 compared with control .A significant P<0.05 decrease in the lymphocytes and monocytes percentage in groups (G3,G4), while no significant decrease P>0.05 in G2 in the lymphocytes and monocytes percentage compared with control. And the results show significant P< 0.05 decrease in HB value in groups G3,G4, while no significant decrease P>0.05 in G2 in HB value compared with control. A significant P< 0.05 decrease was observed in total RBCs counts in groups G2,G3,G4 compared with control. No significant increase P>0.05 in GOT and GPT in groups G2 and G3 and significant P<0.05 increase in GOT and GPT activity in the serum in group G4 compared with control. No significant increase P>0.05 in CPK activity in groups G2,G3,G4 compared with control.

Download Full-text

Effect of Fibrinolytic Microbes and Enzymes on Biochemical Blood Parameters in Crossbred Calves

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.2.5 ◽

2019 ◽

Vol 15 (02) ◽

pp. 18-21

Author(s):

R I Kapadiya ◽

S V Shah ◽

Y G Patel ◽

P R Pandya

Keyword(s):

Blood Parameters ◽

The Other ◽

Control Group ◽

Glutamic Pyruvic Transaminase ◽

Normal Range ◽

Blood Samples ◽

Overall Performance ◽

Similar Body ◽

Control Plasma ◽

Plasma Phosphorus

Twenty crossbred (HF x Kankrej) calves were allotted randomly to five groups (each had one male and three female calves) almost with similar body weight (85.70±6.37 kg) and age (167.55±21.70 days). Experimental calves of the control group were offered hybrid napier untreated, and the other four groups were offered hybrid napier treated with (i) fungus- Aspergillus spp. (1×107 per g feed), (ii) fibrolytic bacteria- Escherichia spp. (106 CFU per g feed), (iii) xylanase (50 mL/kg having xylanase 1.2 IU/mL), and (iv) consortium of 1/3rd dose of fungus + bacteria + enzyme as treatments for 140 days. The blood samples were collected from each calf at a biweekly intervals in the EDTA vacuttee. Nutrients offered to crossbred calves were sufficient to satisfy the nutrients requirements. Bacteria fed calves had significantly higher (plessthan0.05) mean hemoglobin levels than control. Blood plasma glucose of calves in fibrolytic microbes and enzyme groups was statistically similar to control. Feeding of fungus, bacteria, enzyme, and consortium was without a significant effect on plasma total proteins.In comparison to control (7.53 mg/dL), the plasma phosphorus was significantly (plessthan0 0.05) higher in calves fed enzyme (7.87 ± 0.20 mg/dl) and lower in group fed fungus (7.13 mg/dL). In contrast, in bacteria (7.57 mg/dL) and consortium group (7.60 mg/dL) it was statistically similar to control. Plasma glutamic pyruvic transaminase concentration of crossbred calves was lower ( pless than 0.05) in enzyme and higher (p lessthan 0.05) in fungus, bacteria, and consortium groups than in control, while plasma glutamic oxaloacetate transaminase concentration in all groups was statistically similar. In conclusion, all blood parameters of different groups were within normal range and without adverse effect on the overall performance of calves.

Download Full-text

Paracetamol induced hepatic toxicity and amelioration by cinnamon in rats

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v4i2.6529 ◽

2016 ◽

Vol 4 (2) ◽

pp. 187 ◽

Cited By ~ 2

Author(s):

Ashraf Elkomy ◽

Mohamed Aboubakr ◽

Ahmed Soliman ◽

Ahmed Abdeen ◽

Afaf Abdelkader ◽

...

Keyword(s):

Oral Administration ◽

Aqueous Extract ◽

Normal Values ◽

Control Group ◽

Albino Rats ◽

Distilled Water ◽

Hepatic Toxicity ◽

Protective Activity ◽

Group I ◽

Single Oral Administration

The study was designed to evaluate the hepato-protective activity of aqueous extract of cinnamon in acute experimental liver injury induced by paracetamol. Twenty four male albino rats were randomly divided into four groups (six rats in each). Group I rats received distilled water for 15 days and served as a vehicle control. The animals in the group II were given single oral administration of paracetamol (1 g/kg), 1 h after last distilled water administration and acts as paracetamol toxic control group. Groups III and IV received aqueous extract of cinnamon (200 and 400 mg/kg bwt), respectively, once daily for 15 consecutive days followed by a single oral administration of paracetamol (1 g/kg), 1 h after the last cinnamon dose administration. The degree of hepatoprotection was measured using liver enzymes (Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), bilirubin, albumin, and lipid profile levels. Also, histopathological examinations of liver were done. The significantly disturbed liver functions by paracetamol toxicity were restored to nearly normal values by administration of cinnamon. Also, the change in biochemical parameters in groups received paracetamol alone was modified towards the normal values in groups given paracetamol and cinnamon. Histopathological effect of paracetamol on liver was also markedly decreased by co-administration of cinnamon. Our findings concluded that cinnamon aqueous extracts possessed hepato-protective activity against paracetamol induced hepatic toxicity in rats.

Download Full-text

Effect of Quinolones on Serum Testosterone Level in Male Albino Rats

Annals of King Edward Medical University ◽

10.21649/akemu.v23i1.1495 ◽

2017 ◽

Vol 23 (1) ◽

Author(s):

Rubina Iqbal ◽

Saud Iqbal ◽

Shahzad Anjum

Keyword(s):

Testosterone Level ◽

Morphological Changes ◽

Serum Testosterone ◽

Serum Testosterone Level ◽

Term Therapy ◽

Control Group ◽

Albino Rats ◽

Distilled Water ◽

Blood Samples ◽

Testosterone Levels

Objective: To determine the toxic effects of quinolones on serum testosterone level in male albino rats.Methods: Eighty male albino rats were randomly divided into A, B, C and D groups each group having 20 albino rats. These groups were further subdivided into A1, A2, B1, B2, C1, C2, D1 and D2 having 10 albino rats each. Ciprofloxcin, ofloxacin and enoxacin dissolved in distilled water were given at 135mg/kg/ day, 72mg/kg/day and 12.5mg/kg/day to groups A, B and C respectively for 12 weeks. Only distilled water was given to group D which was control group for the same time period. Blood samples were drawn for testosterone hormone level estimation at 0, 14th, 28th and 42nd day in subgroups Al, B1, Cl and D1 and then the animals in said groups were sacrificed on 42nd day to identify testicular morphological changes. Rats in subgroup A2, B2, C2 and D2 were kept alive till 84th day after stopping drugs at 42 days to find out if there is any change in levels of testosterone after discontinuation of the treatment. Blood samples for testosterone hormone estimation were taken at 0, 14th, 28th, 42nd, 56th, 70th and 84th day from subgroups A2, B2, C2 and D2. Testosterone hormone estimation assay was performed on the collected samples.Results: Testosterone assay showed significant decrease in serum testosterone levels in all experimental groups, when compared with control group. These levels did not return back to normal even after withdrawal of drugs. This study suggests a gonadotoxic potential of quinolones.Conclusion: Quinolones reduce serum testosterone levels and should be used carefully for long term therapy.

Download Full-text

Some blood parameters study in albino female mice orally given crude aqueous soy bean (Glycine max) seeds extract

Baghdad Science Journal ◽

10.21123/bsj.8.1.208-214 ◽

2011 ◽

Vol 8 (1) ◽

pp. 208-214

Author(s):

Baghdad Science Journal

Keyword(s):

Blood Cells ◽

Blood Parameters ◽

Control Group ◽

Distilled Water ◽

Water Control ◽

Blood Samples ◽

Fourth Group ◽

Female Mice ◽

Crude Aqueous Extract ◽

Albino Female

The possible effect of the crude aqueous extract of soy bean seeds on some blood parameters (total count of red blood cells, white blood cell , (total and differential) blood platelates, packed cell volume and concentration of blood hemoglobin) was studied in 20 albino female mice which were allocated in four experimental groups (5 mice/group). The first group was orally treated with distilled water (control group) while the second, third and fourth group were given a concentration of 4%, 6% and 8% of the extract, respectively. At the end of the daily gavaging, which lasted for 4 weeks, the animals were killed, after recording their life body weight, and blood samples were collected from each mice to study the effect of the extract on the above mentioned parameters. Some of the active ingredients in the soy bean seeds extract were analytically tested. This test showed that the extract contained flavonoids and saponins. The effect of the extract on the studied blood parameters is reflected by the following results: 1) Significant (P

Download Full-text

RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i1.15399 ◽

2016 ◽

Vol 9 (1) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

V. S. K. Nishihira ◽

N. J. Mezzomo ◽

M. D. Baldissera ◽

R. A. Vaucher ◽

C. G. Pinto ◽

...

Keyword(s):

Inclusion Complex ◽

Oral Administration ◽

Biochemical Parameters ◽

Wistar Rats ◽

Metabolic Diseases ◽

Hematological Parameters ◽

Density Lipoprotein ◽

Blood Parameters ◽

Control Group ◽

Scanning Calorimetry

Objective: The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.Methods: The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.Results: The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P>0.05) similar to the control group.Conclusion: The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.

Download Full-text

Effect of the oral administration of monosodium glutamate during pregnancy and breast-feeding in the offspring of pregnant Wistar rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000100010 ◽

2010 ◽

Vol 25 (1) ◽

pp. 37-42 ◽

Cited By ~ 4

Author(s):

Vinicius von Diemen ◽

Manoel Roberto Maciel Trindade

Keyword(s):

Weight Gain ◽

Oral Administration ◽

Wistar Rats ◽

Monosodium Glutamate ◽

The Other ◽

Control Group ◽

Pregnant Rats ◽

Dependent Relation ◽

Dose Dependent

PURPOSE: Determine the effects of the MSG (monosodium glutamate) in the offspring of pregnant rats through the comparison of the weight, NAL (nasal-anal length) and IL (Index of Lee) at birth and with 21 days of life. METHODS: Pregnant Wistar rats and their offspring were divided into 3 groups: GC, G10 and G20. Each of the groups received 0%, 10% and 20% of MSG, respectively from coupling until the end of the weaning period. RESULTS: Neither weight nor NAL were different among the groups at birth. The group G20 at birth had an IL lower than the group GC (p<0,05) and with 21 days of life presented weight and NAL lower than the groups G10 and this lower than the GC (p<0,01). Otherwise the G20 at 21 days of life had the IL similar to the other two groups. The weight profit percentage from birth to the 21st day of life was lower in the G20 regarding the other two groups (p<0,01). The G20 had a NAL increase percentage from birth to the 21st day of life lower than the G10 and this lower than the GC (p<0,01). CONCLUSIONS: MSG presented a dose-dependent relation in the variables weight and NAL. It caused a decrease in the growth pattern as well as in the weight gain pattern until the 21st day of life. The IL of the group 20% had an increased in relation to the control group after 3 weeks of follow up.

Download Full-text

Evaluation of the Effect of Hydroalcoholic Extracts of Cassia Occidentalis Leaves in Neutrophil Adhesion Test in Rats

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i4-s.3940 ◽

2020 ◽

Vol 9 (4-s) ◽

pp. 1218-1221 ◽

Cited By ~ 1

Author(s):

Dhaval M Patel ◽

Advaita B Patel ◽

Riddhi D Trivedi ◽

Viram J Parmar ◽

Urvisha V Bangoriya

Keyword(s):

Oral Administration ◽

Leukocyte Count ◽

Control Group ◽

Neutrophil Adhesion ◽

Adhesion Test ◽

Differential Leukocyte Count ◽

Hydroalcoholic Extract ◽

Cassia Occidentalis ◽

Blood Samples ◽

Blood Smears

Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis leaves in neutrophil adhesion test in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis leaves on neutrophil has been studied and is compared with control group on rats. The differential leukocyte count (DLC) was performed by fixing the blood smears and staining with leucofine and percent neutrophils in each sample was determined. After the initial counts, blood samples were incubated with 80 mg/ml of nylon fibers for 10 min at 37ºC. Supplementation with hydroalcoholic extract of Cassia occidentalis leaves significantly decreased neutrophil adhesion. The results indicate that the leaf of Cassia occidentalis is endowed with protected neutrophil adhesion. These effects could conclude that Cassia occidentalis has an antiasthmatic property. Keywords: Cassia occidentalis, Neutrophil adhesion, analysis of variance

Download Full-text

Effect of the Clenbuterol drug in some physiological parameters in male rabbits(Oryctolagus cuniculs)

Baghdad Science Journal ◽

10.21123/bsj.13.4.639-648 ◽

2016 ◽

Vol 13 (4) ◽

pp. 639-648

Author(s):

Baghdad Science Journal

Keyword(s):

Kidney Function ◽

Adult Male ◽

Biochemical Study ◽

Study Drug ◽

Physiological Parameters ◽

The Other ◽

Distilled Water ◽

Water Control ◽

Blood Samples

This study was designed to investigate the effect of the Clenbuterol drug in some physiological parameters of Liver, Kidney function and lipidprofie concentration. Fourty adult male rabbits were used in this study. The animals were randomly divided into four groups,the first group was given distilled water (control) ,while the other third groups were given (2,4,6 µg/kg) of Clenbuterol drug for 30 days. Blood samples were collected after 14 and 30 days of the treatment for biochemical study. Drug caused non significant increase(P

Download Full-text

Serum Protein Profile Of Iraqi Hydatidosis Patients with Different Sites of Infection

Baghdad Science Journal ◽

10.21123/bsj.4.4.612-616 ◽

2007 ◽

Vol 4 (4) ◽

pp. 612-616

Author(s):

Baghdad Science Journal

Keyword(s):

Serum Protein ◽

Protein Profile ◽

The Body ◽

The Other ◽

Control Group ◽

Healthy Individuals ◽

Banding Patterns ◽

Blood Samples ◽

Molecular Weights ◽

Serum Protein Profile

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

Download Full-text

380 Effects of estradiol benzoate on reproductive characteristics in beef cows

Journal of Animal Science ◽

10.1093/jas/skz258.296 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 145-145

Author(s):

Lacey M Dysart ◽

Caleb O Lemley ◽

Riley Messman ◽

Jamie E Larson

Keyword(s):

Doppler Ultrasonography ◽

Blood Perfusion ◽

Beef Cows ◽

Estradiol Benzoate ◽

Pregnancy Rates ◽

The Other ◽

Control Group ◽

Blood Samples ◽

Doppler Probe ◽

Imagej Software

Abstract Ovulation prompted by exogenous GnRH may cause the release of sub-mature oocytes and lead to decreased pregnancy rates. Inclusion of estradiol benzoate (EB) has improved pregnancy rates. The objective was to determine if EB affects blood perfusion of follicles, CL, or uterus, concentrations of estradiol, or incidence of standing estrus. Forty-seven suckled beef cows were synchronized (GnRH+CIDR on d -9, PGF2a+CIDR removal on d -2, expected estrus=d 0). On d -1, 24 of 47 cows received an injection of EB (1mg/2mL) while the other 23 remained as a control group. Ovaries were evaluated via Doppler ultrasonography on d -1, 0, 6, 14, and 21. A laser Doppler probe was used to measure blood perfusion of the uterus on d -1, 0, and 6. Only cows that exhibited estrus (n = 29) were evaluated on d 14 and 21. Images were analyzed via ImageJ software to determine mean area of perfusion. Blood samples were collected on d -1 and 0 and analyzed for concentrations of estradiol using RIA. Using SAS, MIXED and FREQ were used to determine the influence of treatment on blood perfusion and estradiol, and incidence of standing estrus, respectively. Treatment with EB increased (P < 0.001) the number of cows exhibiting standing estrus compared to control (21/24 vs 8/23, respectively). Treatment of EB did not (P > 0.10) alter blood perfusion of the follicles or uterus on any day or perfusion of the CL on d 6. However, EB increased (P < 0.036) the size of the CL on d 14 (25.06±1.7 vs 6.06±3.7) and tended (P < 0.097) to increase the size of the CL on d 21. Treatment of EB increased (P < 0.003) concentrations of estradiol on d 0 compared to control (21.35±4.49 vs 9.44±4.61 pg/mL). In conclusion, treatment with EB may play a role in changing the CL after ovulation.

Download Full-text