scholarly journals Effects of 3% binahong (Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats (Rattus norvegicus)

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 923
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Diana Sofia Hanafiah ◽  
Gostry Aldica Dohude ◽  
Denny Satria ◽  
Livita Livita ◽  
...  
Keyword(s):  
Bone Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Biological Activities ◽  
The Other ◽  
Fibroblast Proliferation ◽  
Mandibular Incisor ◽  
Group I ◽  
One Way Anova

Background: Binahong (Anredera cordifolia (Ten.) STEENIS) is a widely available herbal plant in Indonesia and has been intensely researched for its healing abilities due to its biological activities, but few have studied its capability in accelerating hard tissue healing in post-extraction tooth sockets. The purpose of this study was to analyse the effects of 3% binahong leaf extract gel on alveolar bone healing in post-extraction sockets in Wistar rats. Methods: In this study, 48 male Wistar rats were randomly allocated to twelve groups. After the extraction of the left mandibular incisor, sockets in Group I to IV were given 3% binahong leaf extract gel, group V to VIII were given a control gel, and group IX to XII were given Gengigel® for 14 days. The residual socket volume (RSV) and fibroblast proliferation were observed on the 3rd, 7th, and 14th day post-extraction, while the osteoblast and osteocyte proliferation were observed on the 7th, 14th, and 28th day post-extraction. The RSV data were analysed using repeated measure ANOVA and one-way ANOVA, while the histopathological data were analysed using one-way ANOVA. Results: The results showed that the binahong group had the lowest RSV and the highest fibroblast proliferation compared to the other groups on the 7th day (p<0.05) and the highest osteoblast and osteocyte proliferation compared to the other groups on the 14th day (p<0.05). Conclusion: The experiment showed that 3% binahong leaf extract gel could accelerate wound closure, which was characterized by a greater decrease in the RSV value in comparison to the other treatment groups and could enhance alveolar bone healing by increasing the proliferation of fibroblasts, osteoblasts, and osteocytes.

scholarly journals POTENTIALLY OF PURPLE LEAVES TO INCREASE OSTEOBLASTAT ALVEOLAR BONE RAT INDUCED PORPHYROMONAS GINGIVALIS

2021 ◽  
Vol 4 (3) ◽  
pp. 114
Author(s):  
Pramita Wahyu Dyasti ◽  
Atik Kurniawati ◽  
Peni Pujiastuti
Keyword(s):  
Periodontal Disease ◽  
Porphyromonas Gingivalis ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Bone Matrix ◽  
Left Mandible ◽  
Male Wistar Rats ◽  
One Way Anova ◽  
Purple Leaf

Background: Periodontal disease are common dental and oral health problems in the community. Porphyromonas gingivalis (Pg) is one of the main causes of the periodontal disease. The alveolar bone resorption could be as severity indicator of the disease, so that need a material that can help a process of bone remineralization. Osteoblasts are bone-forming cells that are responsible for the mineralization of the bone matrix. Purple leaf is one of the thirteen commodities developed by DitjenPOM as a superior medicinal plant. Purpose: To determine the effect of 2,5%, 5% and 10% purple leaf extract (EDU) of osteoblasts cells in wistar rats induced by Pg. Method: Thirty male wistar rats divided into 5 groups, namely N (without treatment), K (Pg), P (Pg+EDU 2,5%), P2 (Pg+ EDU 5%), P3 (Pg+EDU 10%). EDU administration once a day for 7 days. The mice were decaputated on the 7 th day and the left mandible was taken to make preparations followed by HE staining, observation and calculation of the number of osteoblast cells in 3 different fields. The results of the observations (data) were added up, averaged and analyzed by One Way ANOVA. Result: The group induced by Pg and EDU had a higher average number of osteoblasts than the K and N group. Conclusion: Purple leaf extract (EDU) can increase osteoblasts cells in the alveolar bone of male wistar rats induced by Pg with the highest number of osteoblasts in the 10% concentration.

scholarly journals GAMBARAN HISTOLOGIK AORTA TIKUS WISTAR DENGAN DIET LEMAK BABI SETELAH PEMBERIAN EKSTRAK DAUN PEPAYA

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Friska W. F. Panjaitan ◽  
Marie M. Kaseke ◽  
George N. Tanudjaja
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Group Iv ◽  
Control Group ◽  
Histological Features ◽  
Group I ◽  
Group Ii ◽  
Lard Diet ◽  
Control Group Group

Abstract: Papaya leaves contain several antioxidants (flavonoid, tanin, and vitamin C) which have antiatherogenic effects that may inhibit the progression of an atherosclerotic lesion. One of the risk factors causing atherosclerosis is the consumption of food containing highly saturated fatty acids e.g. lard. Low density lipoproteins (LDL) accumulate within the intima and then are oxidized (LDL-ox). This LDL-ox is ingested by macrophages, resulting in foam-cell formation (early lesion of atherosclerosis). This study aimed to find out the histological features of the aorta of wistar rats having lard diets without the addition of the papaya leaf extract; having lard diets along with the papaya leaf extract; and having lard diets followed by papaya leaf extract. This was an experimental study on 16 wistar rats divided into 4 groups: group I without treatment (negative control group), group II lard diet for 14 days (positive control group), group III lard diet with papaya leaf extract for 14 days (treatment group I), and group IV lard diet for 14 days, and then followed by papaya leaf extract for 14 days (treatment group II). It was found that the aorta of group I showed adipose cells in the intima and media layers; group II and III showed foam cells in both layers; and group IV showed foam cells in fewer numbers than group II. Conclusion: The aorta histological features of wistar rats given lard diets for 14 days, with or without papaya leaf extract, showed foam cells in the intima and media layers. Papaya leaf extraxt added to lard diets had no effect on decreasing foam cells (no protective effect), meanwhile papaya leaf extract following lard diets showed a reduction of foam cells (therapeutic effect). Keywords: papaya leaf, lard dietary, foam cells, wistar rat.   Abstrak: Daun pepaya mengandung antioksidan (flavonoid, vitamin C) yang berefek anti-aterogenik, sehingga diharapkan dapat menghambat perkembangan lesi aterosklerosis. Salah satu faktor risiko penyebab aterosklerosis yaitu makanan yang berkandungan tinggi asam lemak jenuh, antara lain lemak babi. Konsumsi lemak jenuh berlebihan dapat mengganggu fungsi sel endotel, sehingga lipoprotein berdensitas rendah (LDL) dapat masuk dan menjadi LDL teroksidasi (LDL-oks). Makrofag menangkap LDL-oks dan menjadi sel busa (lesi dini aterosklerosis). Penelitian ini bertujuan untuk mengetahui gambaran histologik aorta tikus wistar dengan diet lemak babi tanpa pemberian ekstrak daun pepaya, diet lemak babi bersamaan pemberian ekstrak daun pepaya, dan setelah diet lemak babi dilanjutkan pemberian ekstrak daun pepaya. Penelitian ini bersifat eksperimental. Subyek penelitian terdiri dari 16 ekor tikus wistar yang dibagi menjadi empat kelompok: kelompok I tanpa perlakuan (kelompok kontrol negatif); kelompok II dengan diet lemak babi selama 14 hari (kelompok kontrol positif); kelompok III dengan diet lemak babi serta pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan I); dan kelompok IV dengan diet lemak babi selama 14 hari, dilanjutkan pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan II). Hasil penelitian memperlihatkan gambaran histologi aorta kelompok I tampak perlemakan; pada kelompok II terdapat sel-sel busa; pada kelompok III masih terdapat sel-sel busa; dan pada kelompok IV terdapat sel-sel busa, namun dalam jumlah yang lebih sedikit dibandingkan pada gambaran kelompok II. Simpulan: Tikus wistar dengan diet lemak babi selama 14 hari, baik dengan maupun tanpa ekstrak daun pepaya, memperlihatkan gambaran histologik adanya sel-sel busa pada tunika intima dan tunika media aorta. Pemberian ekstrak daun pepaya bersamaan dengan diet lemak babi tidak berefek menurunkan jumlah sel busa (tidak ada efek protektif) sedangkan pemberian ekstrak daun pepaya setelah diet lemak babi berefek mengurangi jumlah sel-sel busa yang terbentuk (efek terapi). Kata kunci: daun pepaya, diet lemak babi, sel-sel busa, tikus wistar.

scholarly journals Effects of Enoant and Ischemia and Reperfusion on Lens Metabolites of Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Hülya Güngel ◽  
Asiye Nurten ◽  
İhsan Kara ◽  
Serife Evrim Kepekci Tekkeli ◽  
Elif Özkök ◽  
...  
Keyword(s):  
Wistar Rats ◽  
The Other ◽  
Ischemia And Reperfusion ◽  
Polyphenol Content ◽  
Membrane Stabilization ◽  
Group Iii ◽  
Group I ◽  
Ischemia Group ◽  
Dry Diet

The effects of the ischemia and reperfusion on the lens metabolites and the effect of a phytotherapeutic commercial product called “Enoant” (mixed polyphenol content) on the selected lens metabolites were investigated. For this aim, 30 Wistar rats were divided into three groups according to their diet and being subjected to ischemia. 10 of the rats as Group I were fed on dry diet; the other 10 (Group II) were fed on dry diet and drinking water with Enoant. At the end of 15 days period, both groups of rats were subjected to ischemia for 2 hours and reperfused. After another 15 days with their same diet, the rats were decapitated. The remaining 10 rats, which were not subjected to ischemia (Group III), were fed on dry diet only. 1HNMR spectroscopy was used for the determination of lens metabolites of each group of rats. The results obtained from the three groups have been compared statistically. The differences of metabolites were significant except pyruvate and succinate. Oral administration of Enoant revealed effects on increasing membrane stabilization, the antioxidant capacity, osmotic regulator molecule capacity, and sorbitol content of lens disturbed by ischemia. Enoant can be used where oxidative or osmotic stress is formed.

scholarly journals Brine Shrimp Cytotoxic and Lemna minor Phytotoxic Evaluations of the Methanol Extracts of the Leaves, Stem and Root Barks of Sacrocephalus latifolius

2020 ◽  
Vol 27 (2) ◽  
pp. 136-141
Author(s):  
E. O. Ikpefan
Keyword(s):  
Brine Shrimp ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Lemna Minor ◽  
Biological Activities ◽  
Stem Bark ◽  
The Other ◽  
Root Bark ◽  
Artemia Nauplii ◽  
Powder Samples

This work was aimed at evaluating the probable toxicity of the methanol extract of the leaf, stem bark and root of Sacrocephalus latifolius on Artemia nauplii and fronds of Lemna minor. The powder samples of the three morphological parts were independently extracted with soxhlet extractor apparatus using 95% methanol and were dried with aid of ritory evaporator at 40ºC. The extracts were subjected to biological activities involving brine shrimp cytotoxicity and Lemna minor test for phytotoxicity at 10-100 μg /mL respectively. The experiment was done in replicates of three. Concentration-dependent cytotoxic and phytotoxic activities were recorded for the extracts. The results revealed that extract of the leaves demonstrated significant cytotoxic and phytotoxic activities over the other parts of the plant. At 10μg/mL, the three extracts recorded little or no cytotoxic activity. However, at the maximum concentration of 1000 μg /mL, cytotoxicities of 66.67 and 3.33% were recorded for extracts of the leaf and root bark respectively, while the extract of the stem bark showed no activity. However, the LC50 of the leaf was 467.74μg /mL, and that of the other extracts were observed to be ˃1000μg /mL. The results for phytotoxicity followed a similar trend with the extracts of the leaf and root bark recording 52.96 and 23.33 % phytotoxicities respectively at 1000 μg /mL. Having shown a higher activity over the other extracts, the leaf extract of S. latifolius could serves as a natural alternative pesticide and weedicide. Keywords: Sacrocephalus latifolius, phytotoxicity, cytotoxicity, fronds, Artemia nauplii, Lemna minor

scholarly journals Combination of poly L-lactic acid nanofiber scaffold with omentum graft for bone healing in experimental defect in tibia of rabbits

2012 ◽  
Vol 27 (10) ◽  
pp. 694-701 ◽  
Author(s):  
Amir Sotoudeh ◽  
Gholamreza Jahanshahi ◽  
Amirali Jahanshahi ◽  
Mohammad Ashrafzadeh Takhtfooladi ◽  
Iman Shabani ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bone Healing ◽  
Time Course ◽  
The Other ◽  
White Female ◽  
Control Group ◽  
Electrospinning Technique ◽  
Group Iii ◽  
Nanofiber Scaffold ◽  
Group I

PURPOSE: To investigate the osteoconductive properties and biological performance of Poly L-lactic acid (PLLA) with omentum in bone defects. METHODS: PLLA nanofiber scaffolds were prepared via electrospinning technique. Forty four New Zealand white female rabbits randomly divided into three groups of 18 rabbits each. Created defects in right tibias were filled in group I with omentum, in group II with PLLA nanofiber scaffold and in group III with combination of the omentum and PLLA. The same defects were created in left tibia of all groups but did not receive any treatment (control group). Histological and histomorphometric evaluations were performed at two, four and six weeks after the implantation. RESULTS: Histological changes on all groups along with the time course were scored and statistical analysis showed that the average scores in group III were significantly higher than the other groups. CONCLUSION: Histomorphometric analysis of bone healing was shown to be significantly improved by the combined PLLA with omentum compared with the other groups, suggesting this biomaterial promote the healing of cortical bone, presumably by acting as an osteoconductive scaffold.

scholarly journals Effects of Paper Mulberry (Broussonetia papyrifera) Leaf Extract on Growth Performance and Fecal Microflora of Weaned Piglets

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Guoshun Chen ◽  
Shengzhang Shui ◽  
Mingjie Chai ◽  
Dong Wang ◽  
Yingyu Su ◽  
...  
Keyword(s):  
Growth Performance ◽  
Leaf Extract ◽  
The Other ◽  
Control Group ◽  
Immune Functions ◽  
Weaned Piglets ◽  
Broussonetia Papyrifera ◽  
Fecal Microflora ◽  
Group I ◽  
Group Ii

The paper mulberry (Broussonetia papyrifera) leaf is rich in alkaloids and flavonoids, which has high medicinal and feeding value. We aimed to analyze the effects of B. papyrifera leaf extract on growth performance, antioxidant capacity, immune functions, and fecal microflora of weaned piglets. Thirty healthy, 28-day-old piglets were randomly assigned to three groups and fed with a basal diet supplemented with 0, 150, and 300 g/t B. papyrifera leaf extract for 42 days (control group, group I, and group II) separately. The result revealed that the final weight of piglets in group II was higher than the other groups, and the diarrhea rate in this group was 62.9% lower than in the control group. The feed conversion ratio in group I was significantly lower than the other two groups. Higher blood urine nitrogen concentration was noted in group II, higher glutathione peroxidase and catalase in group II, higher superoxide dismutase in the control group, and higher immune globulins (Ig) IgG, IgA, and IgM in group II. There was no significant difference in community richness and community diversity among the three groups of fecal samples. The relative level of Roseburia was higher in groups I and II, while Lactobacillus was higher in the control group. In conclusion, supplementation with B. papyrifera leaf extract at a certain dosage can increase growth performance and antioxidant capacity of weaned piglets, reduce the occurrence of diarrhea, enhance immune functions and disease resistance, and affect the composition of fecal microflora.

scholarly journals ANTI-INFLAMMATORY PROPERTIES OF ZANTHOXYLUM OVALIFOLIUM WEIGHT METHANOLIC EXTRACT ON ALBINO WISTAR RATS

2019 ◽  
pp. 274-277 ◽  
Author(s):  
PAVANI P ◽  
RAJA NAIKA
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Statistical Significance ◽  
Saline Group ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Group I ◽  
Anti Inflammatory

Objective: The object of the present study, the methanolic leaf extract of Zanthoxylum ovalifolium was examined for anti-inflammatory activity and tested using carrageenan-induced paw edema in albino Wistar rats. Materials and Methods: The rats were grouped into five, each group consisting of six animals, Group I (negative control) received 1 ml of saline, Group II (standard) received 10 mg/kg p. o. of indomethacin, and remaining three groups received three different doses (100, 200, and 400 mg/kg p. o.) of the methanolic leaf extract after 1 h 0.1 ml of 1% carrageenan injection. The result of anti-inflammatory activity was set up to be dose-dependent in the carrageenan-induced paw edema model. Results: The methanolic leaf extract has shown significant (p<0.001) inhibition of paw edema, 66%, 66.03%, and 69.29% on the 3rd h at the doses of 100, 200, and 400 mg/kg, p. o., respectively. The results were expressed as the Mean±standard error of mean and statistical significance was analyzed by ANOVA followed by Dunnett’s test. The methanolic leaf of Z. ovalifolium extract showed significant anti-inflammatory activity compared with the standard drug indomethacin. Conclusion: Methanolic leaf extract of Z.ovalifolium showed excellent results in the anti-inflammatory experiment so, the plant can be explored as a potential medicine in suppressing inflammation.

scholarly journals Evaluation of Hepatoprotective effect of hydroalcoholic extract of Momordica Charantia leaves in carbon tetrachloride induced liver toxicity in Wistar rats

2019 ◽  
Vol 12 (3) ◽  
pp. 1555-1560
Author(s):  
Gurudatta Moharir ◽  
Ambadasu Bharatha ◽  
Nkemcho Ojeh ◽  
Vijay Prasad S
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Liver Toxicity ◽  
Liquid Paraffin ◽  
Modern Medicine ◽  
Momordica Charantia ◽  
Control Group ◽  
Group V ◽  
Group I

Liver carries out a variety of physiological functions and protects against damaging drugs and chemicals. Herbs have been shown to play a major role in the management of various liver disorders. Due to the lack of effective liver protective medication in modern medicine, several herbal options for the treatment of liver diseases in Ayurveda are suggested. In this current study, we evaluated the hepatoprotective action of Momordica charantia leaf extract in comparison to Liv-52, a standard hepatoprotective drug. In Wistar rats, hepatotoxicity was induced by administering carbon tetrachloride (CCl4) 1ml / kg body weight subcutaneously on alternate days for a week in a suspension of liquid paraffin. Rats were grouped into 5 groups with group I as control, group II - CCl4 treatment only, group III receiving a mixture of Liv-52 orally (5 ml / kg) and CCl4, and group IV and group V receiving Momordica charantia leaf extract administered orally to rats at doses of 100 and 200 mg / kg respectively, together with CCl4 for 1 week.. Indices of liver functions (lipid profile) were evaluated in the serum of the rats. Animals were sacrificed after the study period and liver tissue was isolated for histopathological changes. The mean results for groups I to V for SGOT levels in IU/L were: 53.57 ± 1.19, 167.72 ± 5.57, 54.72 ± 0.83, 69.41 ± 2.35 and 60.72 ± 1.5 respectively; for SGPT in IU/L were 37.00 ± 1.77, 118.16 ± 2.91, 61.41 ± 1.25, 47.92 ± 1.71 and 58.59 ± 1.81 respectively; for ALP in IU/L were 165.44 ± 4.84, 281.33 ± 7.11, 206 ± 6.95, 190.62±5.47 and 188.86 ± 2.5 respectively and for total bilirubin levels in mg/dl were 0.71± 0.66, 1.57 ± 0.1, 0.80 ± 0.20.88 ± 0.02 & 0.77 ± 0.03 respectively. The findings from this study showed a decrease in the liver enzymes and therefore suggests protective activity of Momordica charantia leaf extract against CCl4 induced hepatic toxicity.

Formulation and Evaluation of Binahong Leaves Extract Gel on Wound Healing of the Palatal Mucosa

2020 ◽  
Vol 48 ◽  
pp. 85-91
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Abidin Trimurni ◽  
Syafrudin Ilyas ◽  
Marline Nainggolan ◽  
Endang Syamsudin
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
Fibroblast Proliferation ◽  
Significance Level ◽  
Accelerate Wound Healing ◽  
Hematoxylin Eosin ◽  
One Way Anova ◽  
Palatal Mucosa

Wound healing is a complex and integrated process that requires the collective roles of various cells and tissues of the palatal mucosa. During the proliferation phase, the sum of fibroblasts increases in response to damaged oral tissues. Thus, the number of fibroblast cells in the palatal mucosa is a substantial indicator of wound healing. Binahong leaves possess the potential to accelerate wound healing by stimulating fibroblast proliferation. This study aimed to formulate and evaluate the effect of Binahong leaves extract (BLE) gel on wound healing of the palatal mucosa by investigating the fibroblast cell count in Wistar rats. This experimental study was carried out in several stages which included the collection and processing of fresh Binahong leaves followed by extraction with 70% ethanol using maceration method. The extract was formulated to be a gel product with a concentration of 3%, 5%, and 7%. BLE gel was administered to Wistar rats which were deliberately injured at the palatal mucosa by excision. Wound healing was assessed using hematoxylin-eosin staining and the number of fibroblast cells was counted. The result of fibroblast proliferation was analyzed by One Way ANOVA followed by Bonferroni’s test with a significance level of 95%. There were significant differences in fibroblast cell proliferation between all test groups except between base gel and Aloclair® gel, base gel and BLE gel 7%, Aloclair® gel and BLE gel 7%, BLE gel 3% and BLE gel 5%. Application of BLE gel 3% promotes better wound healing of the palatal mucosa of Wistar rats.

The Effects of Brotowali (Tinospora crispa) Extract on The Number of Macrophage Cells in Wistar Rats (Rattus norvegicus) Suffering from Periodontitis

2021 ◽  
pp. 3077-3081
Author(s):  
Tuti Kusumaningsih ◽  
Ira Arundina ◽  
Tantiana Tantiana ◽  
Rini Devijanti R ◽  
Indeswati Diyatri ◽  
...  
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Periodontal Tissues ◽  
Macrophage Cells ◽  
Group I ◽  
Significant Difference ◽  
Histological Slide ◽  
Post Hoc ◽  
One Way Anova

Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 ml of 100% brotowali extract (treatment III). The histological slide of each sample then was made to determine the number of macrophages cells. Results: One-Way ANOVA analysis revealed a notable difference among the groups. Tukey HSD Test (Post Hoc Test) showed no significant difference between (treatment group I) and (treatment group II). Conclusion: Brotowali extract may influence macrophage cells count of Wistar rats caused by A. actinomycetemcomitans bacteria.

Sign in / Sign up

Export Citation Format

Share Document