Effect of allicin on wound healing: an experimental diabetes model

2020 ◽  
Vol 29 (7) ◽  
pp. 388-392 ◽  
Author(s):  
Ismail Toygar ◽  
Aynur Tureyen ◽  
Derya Demir ◽  
Sevki Cetinkalp
Keyword(s):  
Wound Healing ◽  
Experimental Diabetes ◽  
Diabetic Rats ◽  
Controlled Study ◽  
Future Research ◽  
Albino Rats ◽  
Wound Area ◽  
Diabetes Model ◽  
Physiological Serum ◽  
And Control

Objective: The aim of this study was to investigate the effect of allicin on wound healing in an experimental diabetes model. Method: In this randomised controlled study, 50 Wistar albino rats (25 females, 25 males) each weighing 200–300g were used. To develop the diabetes model, 30 rats were induced with 50mg/kg streptozotocin (STZ); 20 rats were not induced in order to compare diabetic and nondiabetic rats. The diabetic rats were divided into three groups, according to dressing material used (allicin, physiological serum and control, where no dressing was used), and the nondiabetic rats were divided into two groups (allicin and control, where no dressing was used). The wound area was calculated and recorded on days 0, 7, 14 and 21. In addition, biopsies were taken from the wound area on days 0, 7, 14 and 21 and used for microscopic examination. Day 0 was used as a reference to calculate wound healing percentage. Results: On days 7 and 14, there were statistically significant differences between groups. Wound surface areas were smaller in the allicin group than in other groups on days 7 and 14. There were no statistically significant differences between the groups on day 21. In addition, it was determined that neutrophil, mononuclear cell, intraepithelial oedema and dermal oedema density were lower and fibroblast, angiogenesis and collagen density were higher in the allicin groups on days 7 and 14. Conclusion: In this study, allicin was found to be potentially effective on wound healing. Future research should be conducted in order to clarify how it affects wound healing.

scholarly journals Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Linhao Wang ◽  
Fang Wang ◽  
Liling Zhao ◽  
Wenjun Yang ◽  
Xinxing Wan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Diabetic Rats ◽  
Skin Thickness ◽  
Wound Area ◽  
Promote Wound Healing ◽  
Diabetes Model ◽  
Diabetic Wound Healing

Objective. To investigate the effects of mesenchymal stem cells (MSCs) coated by the extracellular matrix (ECM) on wound healing in diabetic rats. Methods. Mesenchymal stem cells were cocultured with ECM. Cell viabilities were evaluated using MTT assay. The diabetes model was established using both STZ and high-glucose/fat methods in SD rats. A wound area was made on the middle of the rats’ back. MSCs or ECM-MSCs were used to treat the rats. HE staining and CD31 immunohistochemistry were used to detect the skin thickness and angiogenesis. Western blotting and qRT-PCR were conducted to determine the level of VEGF-α, PDGF, and EGF. Results. It was observed that treatment of ECM had no significant effects on the cell viability of ECM-MSCs. Wound area assay showed that both MSCs and ECM-MSCs could enhance the wound healing of diabetic rats and ECM-MSCs could further promote the effects. Both MSCs and ECM-MSCs could enhance angiogenesis and epithelialization of the wounds, as well as the expression of VEGF-α, PDGF, and EGF in wound tissues, while ECM-MSC treatment showed more obvious effects. Conclusion. Mesenchymal stem cells coated by the extracellular matrix could promote wound healing in diabetic rats. Our study may offer a novel therapeutic method for impaired diabetic wound healing.

Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

2020 ◽  
Vol 29 (9) ◽  
pp. 488-495
Author(s):  
Amene Nikgoftar Fathi ◽  
Mohammad Hassan Sakhaie ◽  
Sepehr Babaei ◽  
Soroush Babaei ◽  
Fateme Slimabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Wound Area ◽  
Small Vessels ◽  
Histological Sections ◽  
Therapeutic Benefits ◽  
Wide Range

Objective: To assess the effect of bromelain on different aspects of the wound healing process in type 1 diabetic rats. Method: In this study, 112 streptozocin-diabetic (type 1) male Wistar rats were euthanised; 28 each on days three, five, seven and 15, after a wound incision had been made. To estimate changes in a number of different cellular and tissue elements, histological sections were provided from all wound areas and stained with haematoxylin and eosin. Some 1.056mm2 of total wound area from all specimens were evaluated, by assessment of 4200 microscope photos provided from all histological sections, by stereological methods. A biomechanical test of each wound area was performed with an extensometer to evaluate the work-up to maximum force and maximum stress of the healed wound on day 15. Results: In the experimental groups, bromleain caused significant wound contraction and reduced granulation tissue formation by day 7 (p=0.003); increased neovasculars (new small vessels that appear in the wound area during wound healing) on days three, five and seven (p=0.001); significantly increased fibroblasts on day five but decreased by day seven (p=0.002); and significantly decreased macrophage numbers and epithelium thickness on all days of study (p=0.005). Wound strength significantly increased in experimental groups by day 15. Conclusion: Bromelain has a wide range of therapeutic benefits, but in most studies the mode of its action is not properly understood. It has been proved that bromelain has no major side effects, even after prolonged use. According to the results of this study, bromelain can be used as an effective health supplement to promote and accelerate wound healing indices, reduce inflammation and improve biomechanical parameters in diabetic wounds.

scholarly journals Blocking connexin 43 accelerates corneal healing and improves tissue remodeling during the healing of diabetic rat corneas: A histological and immunohistochemical study

2019 ◽  
Vol 17 ◽  
pp. 205873921984338
Author(s):  
Hossein M Elbadawy ◽  
Amany Elshawarby ◽  
Mona H Raafat ◽  
Nevine Bahaa ◽  
Mohi IM Abdul ◽  
...  
Keyword(s):  
Wound Healing ◽  
Connexin 43 ◽  
Tissue Remodeling ◽  
Inflammatory Cells ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Albino Rats ◽  
Corneal Tissue ◽  
Cell Degeneration ◽  
Corneal Injury

Connexin 43 (Cx43) is a potential target for accelerating wound healing. This study aimed at evaluating the therapeutic efficiency of topical application of Gap27, a Cx43 mimetic peptide, on corneal tissue reorganization during wound healing in streptozocin-induced Diabetes in albino rats and its effect on the infiltration of inflammatory cells. Fifty adult male albino Wistar rats were divided equally into two groups: non-diabetic and diabetic. Twenty rats from each group were subjected to corneal injury: 10 untreated and 10 treated with Gap27. The remaining five rats from each group served as negative controls (intact corneas). All rats were sacrificed 3 days after injury. Histological studies were performed to assess signs of cell degeneration, the infiltration of inflammatory cells. Histomorphometric studies were performed to quantify the expression of Cx43. Gap27 promoted corneal wound healing in non-diabetic and diabetic rats. It reduced mononuclear cell infiltration and improved corneal tissue remodeling. However, minor structural changes were still seen in diabetic corneas after treatment with Gap27. Blocking Cx43 was a valuable tool to restore corneal tissue structure, reduce the infiltration of inflammatory cells in non-diabetic and diabetic rats.

scholarly journals Wound Healing Study of Panchavalkal Ointment in Wistar Albino Rats

2021 ◽  
pp. 110-116
Author(s):  
Vaikhari Dhurve ◽  
Pravin Jawanjal ◽  
Mukesh Naria ◽  
Tukaram Dudhamal ◽  
Minal Kalambe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Wound Area ◽  
Mean Values ◽  
Wistar Strain ◽  
The Mean ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

Introduction: Panchavalkal is a well-known Ayurvedic poly-herbal formulation that has been reported to be used against inflammation, to clean ulcer, wound. Aims and Objectives: To investigate the wound healing activity of Panchavalkal ointment. Materials and Methods: Wistar strain albino rats of either sex weighing 200±20 g were used for the experiments divided in four groups each consisted of six rats.  Statistical Analysis: One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variables among the groups followed by Dunnett’s multiple ‘t’ test Observations & Results: Sesame oil and Panchvalkal ointment showed almost similar wound healing effects in comparison to control group. Discussion: Panchavalkal ointment showed statistically highly significant percentage of contraction of excision wound area compared to the normal control. Epithelization period was significantly decreased in oil and Panchavalkal ointment treated group. Conclusion: Panchavalkal ointment decreased the pain, tenderness, redness and swelling that helps to control infection and enhanced the rate of wound healing in albino rats.

scholarly journals Evaluation of Antidiabetic Potentials of Morus mesozygia Linn. Stapf., Leaf Extracts in Streptozotocin-Induced Diabetic Rats

2020 ◽  
pp. 23-32
Author(s):  
Marcella Tari Joshua ◽  
Edna O. Wachuku ◽  
N. Boisa ◽  
Nsirim Nduka
Keyword(s):  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Male Rats ◽  
Controlled Study ◽  
Albino Rats ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Citrate Buffer ◽  
Standard Drug ◽  
C Peptide

Aim: The aim of this study therefore was to evaluate the antidiabetic potentials of Morus mesozygia Linn. Stapf., Leaf extrcts in Streptozotocin-Induced Diabetic Rats. Study Design:  The study is an experimental case-controlled study. Place and Duration of Study: This study was carried out at the Biochemistry Research Laboratory, University of Port Harcourt, Rivers State, Nigeria, between June 2018-April 2019. Methodology: A total of 65 male albino rats that weighed between 150g to 200g were used for this research study. Three different extracted solvents; aqueous, ethanolic and methanolic leaves extracts were administered to different groups of the rats. The male albino rats for this study were induced with a single dose of 40mg/kg b.wt, intraperitoneally of streptozotocin in 0.1M of citrate buffer, pH 4.5. The diabetic male rats were those whose fasting blood glucose (FBG) was from 250 mg/dl or 13 mmol/L and above. Results: The results showed that there were significant decreases (p<0.05) in, FBG, increased insulin and increased C-peptide and increased HOMA-IR concentration of induced diabetic male rats treated orally with 400 mg/kg of MMLS., when compared with the values of the diabetic male rats in treated orally with 200 mg/kg of MMLS., and non-significant decrease(p<0.05) in FBG, HOMA-IR increased insulin, increased C-peptide concentrations in the diabetic male rats treated orally for 30 days with 400 mg/kg in dose of methanolic leaves extracts of MMLS., in the group treated with 400 mg/kg methanol, when compared to the concentration of diabetic male in treated orally for 30 days with 200 mg/kg of methanolic leaves extracts of MMLS. Compared likewise with that treated with 100mg/kg of metformin standard drug. There were also significant decrease (p<0.05) in FBG, HOMA-IR, C-peptide and increased insulin concentrations in diabetic male rats treated with 200 mg/kg in dose of ethanolic and methanolic leaves extracts of MMLS., when compared with the values in the diabetic male rats treated orally with 400 mg/kg in dose of ethanolic and methanolic leaves extracts of MMLS., compared with values of diabetic male rats treated orally with 100mg/kg in dose of metformin standard drugs compared with the controls. Conclusion: From the findings of this study, we conclude that streptozotocin increased FBG levels, while the Methanolic, ethanolic and aqueous extracts of Morus mesozygia Linn. S improved FBG, C-peptide, insulin and HOMA levels in a dose-dependent manner, with the methanolic extract having the best ameliorative effect, probably due to its more phytochemical composition.

Platelet Abnormalities in Experimental Diabetes

1979 ◽  
Author(s):  
M. Johnson ◽  
H.E. Harrison ◽  
R. Hawker ◽  
L. Hawker
Keyword(s):  
Platelet Function ◽  
Experimental Diabetes ◽  
Platelet Reactivity ◽  
Vascular Complications ◽  
Diabetic Rats ◽  
Thromboxane Synthetase ◽  
Patients With Diabetes ◽  
Streptozotocin Diabetic Rats ◽  
And Control

Many abnormalities of platelet function occur in patients with diabetes mellitus, partioularly those with angiopathy. We have previously demonstrated that prostacyclin (PGI2) is decreased in streptozotocin-diabetic rats, and have now investigated platelet reactivity in these animals. Responsiveness to ADP and arachidonlc acid was increased, and platelet cyclo-oxygenase and thromboxane synthetase activities were significantly elevated (p<0.05) in diabetic animals (5.5±0.7 and 5.9±0.9 arbitrary units/109 platelets) when compared with control animals (J.0±0.4 and 3.9±0.3 arbitrary units/109 platelets). Malondialdehyde synthesis was 1,5 and 0.9 n moles per 108 platelets in diabetic and control rats respectively. Diabetic platelets were also less sensitive to the anti-aggregating effects of PGI2 (IC50: diabetic, 2.3 ng/ml; control, 1.3 ng/ml. Survival of illindium-labelled autologous platelets was significantly reduced, indicating that platelet function is abnormal in vivo, in diabetic animals. Platelet hyper-reactivity, possibly associated with depressed PGI2, could be related to the vascular complications of diabetes.

scholarly journals The Wound Healing Effect of Nanoclay, Collagen, and Tadalafil in Diabetic Rats: An In Vivo Study

2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Allahyar Noori Ordeghan ◽  
Danial Khayatan ◽  
Mohammad Reza Saki ◽  
Mostafa Alam ◽  
Kamyar Abbasi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inhibitory Effect ◽  
Exercise Group ◽  
Diabetic Rats ◽  
Hair Follicles ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Management Approaches ◽  
And Control

The diabetic wound is the most challenging one to manage, which is associated with microvascular and macrovascular dysfunction, and novel strategies such as using hydrogels demonstrate their promising prospect in treatment and management approaches of the diabetic wound. This study aimed to investigate the effect of collagen/nanoclay/tadalafil hydrogel on wound healing in diabetic rats under HIIT exercise. Hydrogel was synthesized, and then biocompatibility and antibacterial tests were performed. The therapeutic effect of collagen/nanoclay/tadalafil hydrogel was assessed after induction of diabetes in the rat model, and wound healing was evaluated with macroscopic and microscopic tests. The result of the MTT test showed no significant cytotoxicity of collagen/nanoclay/tadalafil hydrogel. Furthermore, the inhibitory effect of hydrogel was detected on E. coli and S. aureus. The macroscopic results demonstrated that the wound contraction was considerable in the hydrogel/HIIT exercise and hydrogel groups compared with the HIIT exercise and control groups during 21 days. The microscopic results showed that the presence of fibroblasts, the amount of collagen, the epidermis density, and the formation of hair follicles were increased in the hydrogel/HIIT exercise group compared with other groups in the diabetic rate model. It can be concluded that collagen/nanoclay/tadalafil hydrogel with HIIT exercise could accelerate diabetic wound healing and can be an appropriate candidate for skin regeneration in medical applications.

scholarly journals Therapeutic Effects of Static Magnetic Field on Wound Healing in Diabetic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Jing Zhao ◽  
Yong-guo Li ◽  
Kai-qin Deng ◽  
Peng Yun ◽  
Ting Gong
Keyword(s):  
Magnetic Field ◽  
Wound Healing ◽  
Static Magnetic Field ◽  
Wound Closure ◽  
Reduction Rate ◽  
Diabetic Rats ◽  
Control Group ◽  
Exposure Group ◽  
Wound Area ◽  
Area Reduction

Objective. To investigate the effects of static magnetic field (SMF) on cutaneous wound healing of Streptozotocin- (STZ-) induced diabetic rats.Methods. 20 STZ-induced diabetic rats were randomly divided into two groups (10 in each group): diabetic rats with SMF exposure group which were exposed to SMF by gluing one magnetic disk of 230 mT intensity and diabetic rats with sham SMF exposure group (sham group). 10 normal Wistar rats were used as the control group. One open circular wound with 2 cm diameter in the dorsum was generated on both normal and diabetic rats and then covered with sterile gauzes. Wound healing was evaluated by wound area reduction rate, mean time to wound closure, and wound tensile strength.Results. The wound area reduction rate in diabetic rats in comparison with the control group was significantly decreased (P<0.01). Compared with sham magnet group, diabetic rats under 230 mT SMF exposure demonstrated significantly accelerated wound area reduction rate on postoperative days 7, 14, and 21 and decreased gross time to wound closure (P<0.05), as well as dramatically higher wound tissue strength (P<0.05) on 21st day.Conclusion. 230 mT SMF promoted the healing of skin wound in diabetic rats and may provide a non-invasive therapeutic tool for impaired wound healing of diabetic patients.

Alterations in alpha1-adrenoceptor stimulation of isolated atria from experimental diabetic rats

1986 ◽  
Vol 64 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Charles V. Jackson ◽  
Gail M. McGrath ◽  
John H. McNeill
Keyword(s):  
Experimental Diabetes ◽  
Receptor Activation ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Beta Adrenoceptor ◽  
Chemically Induced ◽  
Isolated Atria ◽  
And Control ◽  
Right Atria ◽  
Stimulation Of

This purpose of this investigation was to determine the influence of experimental diabetes (3 months) on the responsiveness of rat isolated atria to alpha1-adrenoceptor stimulation by phenylephrine. Diabetes was chemically induced with streptozotocin (65 mg/kg i.v.) in 42- to 43-day-old, nonfasted male Sprague–Dawley derived rats. Chronotropic (right atria) and inotropic (left atria) indices were recorded in response to alpha1-adrenoceptor stimulation by phenylephrine. These experiments were performed in the presence of beta-adrenoceptor antagonism (timolol). Isolated right atria from diabetic rats demonstrated a greater increase in heart rate in response to phenylephrine than did corresponding control atria. Left atria were supersensitive (decrease in EC50 values) and hyperresponsive to alpha1-adrenoceptor stimulation by phenylephrine when compared with stimulation of control left atria. Diabetic left atria in response to phenylephrine were observed to exchange more radioactive calcium (45Ca2+) than control left atria, whereas both diabetic and control left atria exchanged the same amount of 45Ca2+ during basal contractile conditions. Phenylephrine had no effect on 45Ca2+ efflux from either diabetic or control atria. These results indicate that 3 months of uncontrolled experimental diabetes in the rat produces an enhancement of alpha1-adrenoceptor activation of isolated atria, and that there is an alteration in Ca2+ mobilization which may contribute to the enhanced receptor activation.

Melatonin, quercetin and resveratrol attenuates oxidative hepatocellular injury in streptozotocin-induced diabetic rats

2015 ◽  
Vol 34 (9) ◽  
pp. 859-868 ◽  
Author(s):  
H Elbe ◽  
M Esrefoglu ◽  
N Vardi ◽  
E Taslidere ◽  
E Ozerol ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Experimental Diabetes ◽  
Periodic Acid ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Hepatocellular Injury ◽  
Diabetes Group ◽  
Periodic Acid Schiff ◽  
Glucose Levels ◽  
Blood Glucose Levels

In this study, effects of melatonin, quercetin and resveratrol on hepatocellular injury in streptozotocin (STZ)-induced experimental diabetes were aimed to be investigated by histological and biochemical methods. Thirty-five male Wistar albino rats were divided into five groups, namely, control, diabetes (STZ 45 mg/kg/single dose/intraperitoneally (ip)), diabetes + melatonin (10 mg/kg/30 days/ip), diabetes + quercetin (25 mg/kg/30 days/ip) and diabetes + resveratrol (10 mg/kg/30 days/ip). Initial and final blood glucose levels and body weights (BWs) were measured. At the end of the experimentation, following routine tissue processing procedure, sections were stained with haematoxylin–eosin (H-E), periodic acid Schiff and Masson’s trichrome. Tissue malondialdehyde (MDA) and glutathione (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities were examined. The diabetic rats had significantly higher blood glucose levels than those of control rats ( p = 0.0001). Mean BWs of diabetic rats were significantly decreased when compared with the control rats ( p = 0.0013). Histopathological alterations including cellular glycogen depletion, congestion, sinusoidal dilatation, inflammation and fibrosis were detected in diabetes group. On the other hand, histopathological changes markedly reduced in all of the treatment groups ( p = 0.001). Mean tissue MDA level was increased but mean tissue CAT and SOD activities and GSH levels were decreased in the diabetes group. Melatonin, quercetin and resveratrol administered diabetic rats showed an increase in CAT activities and GSH levels and a decrease in MDA levels ( p < 0.05, for all). Melatonin, quercetin and resveratrol administrations markedly reduced hepatocellular injury in STZ-induced experimental diabetes.

Sign in / Sign up

Export Citation Format

Share Document