scholarly journals Blocking connexin 43 accelerates corneal healing and improves tissue remodeling during the healing of diabetic rat corneas: A histological and immunohistochemical study

2019 ◽  
Vol 17 ◽  
pp. 205873921984338
Author(s):  
Hossein M Elbadawy ◽  
Amany Elshawarby ◽  
Mona H Raafat ◽  
Nevine Bahaa ◽  
Mohi IM Abdul ◽  
...  
Keyword(s):  
Wound Healing ◽  
Connexin 43 ◽  
Tissue Remodeling ◽  
Inflammatory Cells ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Albino Rats ◽  
Corneal Tissue ◽  
Cell Degeneration ◽  
Corneal Injury

Connexin 43 (Cx43) is a potential target for accelerating wound healing. This study aimed at evaluating the therapeutic efficiency of topical application of Gap27, a Cx43 mimetic peptide, on corneal tissue reorganization during wound healing in streptozocin-induced Diabetes in albino rats and its effect on the infiltration of inflammatory cells. Fifty adult male albino Wistar rats were divided equally into two groups: non-diabetic and diabetic. Twenty rats from each group were subjected to corneal injury: 10 untreated and 10 treated with Gap27. The remaining five rats from each group served as negative controls (intact corneas). All rats were sacrificed 3 days after injury. Histological studies were performed to assess signs of cell degeneration, the infiltration of inflammatory cells. Histomorphometric studies were performed to quantify the expression of Cx43. Gap27 promoted corneal wound healing in non-diabetic and diabetic rats. It reduced mononuclear cell infiltration and improved corneal tissue remodeling. However, minor structural changes were still seen in diabetic corneas after treatment with Gap27. Blocking Cx43 was a valuable tool to restore corneal tissue structure, reduce the infiltration of inflammatory cells in non-diabetic and diabetic rats.

scholarly journals The acute effects of different spironolactone doses on cardiac function in streptozotocin-induced diabetic rats

2017 ◽  
Vol 95 (11) ◽  
pp. 1343-1350
Author(s):  
Aleksandra Vranic ◽  
Stefan Simovic ◽  
Petar Ristic ◽  
Tamara Nikolic ◽  
Isidora Stojic ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Systolic Function ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Albino Rats ◽  
Acute Administration ◽  
Rat Hearts ◽  
Patients With Diabetes ◽  
Streptozotocin Induced Diabetes ◽  
Wistar Albino Rats

Currently, cardiovascular diseases are the leading cause of global mortality, while diabetes mellitus remains an important cause of cardiovascular morbidity. A recent study showed that patients with diabetes mellitus treated with mineralocorticoid receptor antagonists have improved coronary microvascular function, leading to improved diastolic dysfunction. In this study, we evaluated the influence of acute administration of spironolactone on myocardial function in rats with streptozotocin-induced diabetes mellitus, with special emphasis on cardiodynamic parameters in diabetic rat hearts. The present study was carried out on 40 adult male Wistar albino rats (8 weeks old). Rats were randomly divided into 4 groups (10 animals per group): healthy rats treated with 0.1 μmol/L of spironolactone, diabetic rats treated with 0.1 μmol/L of spironolactone, healthy rats treated with 3 μmol/L of spironolactone, and diabetic rats treated with 3 μmol/L of spironolactone. Different, dose-dependent, acute responses of spironolactone treatment on isolated, working diabetic and healthy rat heart were observed in our study. In healthy rats, better systolic function was achieved with higher spironolactone dose, while in diabetic rats, similar effects of low and high spironolactone dose were observed.

Antioxidant, anti-inflammatory, and anti-apoptotic effects of zinc supplementation in testes of rats with experimentally induced diabetes

2018 ◽  
Vol 43 (10) ◽  
pp. 1010-1018 ◽  
Author(s):  
Neven M. Aziz ◽  
Maha Y. Kamel ◽  
Manar S. Mohamed ◽  
Sabreen M. Ahmed
Keyword(s):  
Connexin 43 ◽  
Concomitant Administration ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Safe Alternative ◽  
Once Daily ◽  
Factor Α ◽  
Percent Area

One of the major obstacles that males with diabetes may confront is subfertility or infertility. Thus, the present study investigated the effect of co-administration of metformin and zinc (Zn) on the testes of streptozotocin-induced diabetic rats. Male albino rats were randomly divided into 4 groups: control group; untreated diabetic group; diabetic + metformin group, in which diabetic rats were treated orally with metformin (250 mg/kg) once daily for 4 weeks; and diabetic + metformin + Zn group, in which diabetic rats were treated orally with metformin in combination with Zn (10 mg/kg) once daily for 4 weeks. Concomitant administration of metformin and Zn produced a significant decrease in serum levels of glucose and insulin and testicular levels of malondialdehyde and tumor necrosis factor α. Additionally, there was a significant increase in serum levels of Zn, testosterone, and follicle-stimulating hormone, as well as testicular total antioxidant capacity and anti-apoptotic protein Bcl-2, when compared with both the diabetic and metformin-treated diabetic groups. Moreover, co-administration of Zn and metformin significantly improved testicular histopathology, with a significant reduction in percent area of collagen fibers and nuclear factor kappa B (p65) immunoreactivity and a significant increase in seminiferous tubule diameter and connexin 43 immunoreactivity as compared with the diabetic and metformin-treated diabetic groups. In conclusion, the combination of Zn and metformin was an efficacious and safe alternative treatment, as it had superior antihyperglycemic efficacy and provided additional benefits over metformin alone in rats with type 2 diabetes.

scholarly journals Preparation of W/O Hypaphorine–Chitosan Nanoparticles and Its Application on Promoting Chronic Wound Healing via Alleviating Inflammation Block

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2830
Author(s):  
Mengting Qi ◽  
Xuerui Zhu ◽  
Xiaoyi Yu ◽  
Min Ai ◽  
Weiwei Cai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Chitosan Nanoparticles ◽  
Average Diameter ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Patients With Diabetes ◽  
Treatment Of Wounds

Chronic wound repair is a common complication in patients with diabetes mellitus, which causes a heavy burden on social medical resources and the economy. Hypaphorine (HYP) has good anti-inflammatory effect, and chitosan (CS) is used in the treatment of wounds because of its good antibacterial effect. The purpose of this research was to investigate the role and mechanism of HYP-nano-microspheres in the treatment of wounds for diabetic rats. The morphology of HYP-NPS was observed by transmission electron microscopy (TEM). RAW 264.7 macrophages were used to assess the bio-compatibility of HYP-NPS. A full-thickness dermal wound in a diabetic rat model was performed to evaluate the wound healing function of HYP-NPS. The results revealed that HYP-NPS nanoparticles were spherical with an average diameter of approximately 50 nm. The cell experiments hinted that HYP-NPS had the potential as a trauma material. The wound test in diabetic rats indicated that HYP-NPS fostered the healing of chronic wounds. The mechanism was through down-regulating the expression of pro-inflammatory cytokines IL-1β and TNF-α in the skin of the wound, and accelerating the transition of chronic wound from inflammation to tissue regeneration. These results indicate that HYP-NPS has a good application prospect in the treatment of chronic wounds.

scholarly journals A proteomics approach to identify the differential protein level in cardiac muscle of diabetic rat.

2014 ◽  
Vol 61 (2) ◽  
Author(s):  
Dhanaraj Karthik ◽  
Ravichandran Vijayakumar ◽  
Kalailingam Pazhanichamy ◽  
Sivanesan Ravikumar
Keyword(s):  
Cynodon Dactylon ◽  
Diabetic Complication ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Albino Rats ◽  
The Novel ◽  
Group Iii ◽  
Group I ◽  
Proteomics Approach

Cardiovascular proteomics investigation reveals the characterization and elucidation of the novel therapeutic targets and strategies to prevent the development of heart failure associated diabetic complication by using 2DE and MS. The experimental animals were made diabetic with a single intraperitoneal injection of alloxan (150 mg/kg of bw). Albino rats were randomly divided into four individual groups: Group-I control (n=6), group-II alloxan-induced diabetic rats, untreated (n=6), group-III (n=6) and group-IV (n=6) alloxan-induced diabetic rats were treated with aqueous and ethanolic extracts of Cynodon dactylon for 15 days, respectively. Animals were euthanized to collect the heart tissues and blood samples. 2DE sample preparation, gel running and staining (n=6: each groups) were performed at the same time to avoid variation. The result of six gel images from each group were analyzed and evaluated as one match set with 2D software (P

scholarly journals ALOE IRAFENSIS AN ENDEMIC PLANT OF YEMEN: PHYTOCHEMICAL SCREENING, ANTIBACTERIAL, ANTIOXIDANT, AND WOUND-HEALING ACTIVITIES

2021 ◽  
pp. 88-94
Author(s):  
BUSHRA ABDULKARIM MOHARRAM ◽  
HASSAN M. AL-MAHBASHI ◽  
TAREQ AL-MAQTARI ◽  
RIYADH SAIF-ALI ◽  
AMIN A. A-DOAISS
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Radical Scavenging ◽  
Inflammatory Cells ◽  
Albino Rats ◽  
Histopathological Study ◽  
Endemic Plant ◽  
Methanol Extracts ◽  
Phytochemical Constituents ◽  
Free Radical Scavenging Assay

Objectives: The objectives of the study were to determine the phytochemical constituents and assess the antibacterial, antioxidant, and wound-healing properties of the methanol extracts of Aloe irafensis. Methods: Methanol extracts of A. irafensis’s latex, gel, and green skin were screened for their phytochemical constituents. All three extracts were investigated regarding their antibacterial potential using disc diffusion and microdilution assays, and their antioxidant activity using 2,2-diphenyl-1- picrylhydrazyl free-radical scavenging assay. Histopathological study of wound healing area was performed for the latex extract in male albino rats. Results: The methanol extracts of A. irafensis revealed the presence of carbohydrates, steroids, phenols, tannins, and anthrones. The latex extract showed greater inhibition zones against Staphylococcus aureus and Pseudomonas aeruginosa (24 and 17 mm, respectively) and minimum inhibitory concentration values of 1.25 and 2.50 mg/ml, respectively. The latex extract showed the highest antioxidant activity (IC50 of 65.54 μg/ml), followed by green skin (IC50 of 89.48 μg/ml). The latex extract significantly accelerated the rate of wound healing in rats (p<0.01), compared to fucidin ointment, a reference control. Histological findings showed remarkably less scar width at wound closure site in the latex extract-treated wounds. Granulation tissue contained fewer inflammatory cells and more fibroblasts in wounds treated with the latex extract compared to those treated with the vehicle. Conclusion: A. irafensis latex extract is a potential source of bioactive compounds that can be used as antioxidant, antibacterial, and wound healing agents.

scholarly journals EFFECTS OF CINNAMON EXTRACT IN DIABETIC RAT MODELS IN COMPARISON WITH ORAL HYPOGLYCEMIC DRUGS

2018 ◽  
Vol 21 (04) ◽  
pp. 717-722
Author(s):  
Muhammad Sajid Khan ◽  
Aneela Qureshi ◽  
Shuja Anwar Kazi ◽  
Amin Fahim ◽  
Husan Bano ◽  
...  
Keyword(s):  
Low Dose ◽  
Treated Group ◽  
Diabetic Animal ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Albino Rats ◽  
P Value ◽  
Medical College ◽  
Cinnamon Extract ◽  
Oral Hypoglycemic Drugs

Objective: To evaluate the anti diabetic effect of cinnamon extract in alloxaninduced diabetic animal model (albino rats) in comparison with oral hypoglycemic drugs. StudyDesign: An Experimental study. Place of Study: Al Tibri Medical College, Isra University, KarachiCampus. Duration of Study: December 2012 to December 2013. Materials and Methods: Total60 Albino rats of both genders were divided into 6 groups consisting of 10 rats in each group.Each group of animals was further divided into two sub groups containing 5 rats in each groupResults: The results obtained from the data indicated that there is significant reduction in bloodglucose level rats treated with low dose of cinnamon extract. The animals of low dose cinnamonextract (200mg/kg. bw) when compared with other groups; there is a reduction in the bloodglucose level in alloxan induced diabetic rats. Also tolbutamide and acarbose treated groupsshowed better antidiabetic effects as compared with cinnamon extract treated groups (pvalue<0.007 and p value<0.012 respectively), but cinnamon extract treated group showedsynergetic effects when it was given in combination with tolbutamide or acarbose havingsignificant p value<0.001 and p value<0.011 respectively. Conclusions: Tolbutamide andAcarbose showed better anti diabetic effect in comparison with cinnamon extract treated groupswhen used individually. This effect was enhanced when cinnamon was used in combination witheither tolbutamide or acarbose.

scholarly journals Subacute Toxicity and Hepatoprotective Effects of Sarcocephalus latifolius in Alloxan Induced Diabetic Rats

2021 ◽  
pp. 24-37
Author(s):  
Olubunmi Simeon Oyekunle ◽  
Adewale Adetutu ◽  
Adijat Funke Ogundola
Keyword(s):  
Liver Disease ◽  
Low Dose ◽  
Diabetes Management ◽  
Inflammatory Cells ◽  
Diabetic Rats ◽  
The Body ◽  
Albino Rats ◽  
Diabetic Patients ◽  
High Dose ◽  
Leaf Powder

Various studies suggest that mortality due to liver disease in diabetic patients is very high; however, the recognition of DM as the primary cause of chronic liver disease is neglected in medical practice, we therefore evaluated the activities of Sarcocephalus latifolius leaf powder on the liver function of alloxan – induced diabetic rats. Forty-five healthy female albino rats were randomly assigned into 9 different groups; diabetes was induced intraperitonealy with 160 mg/kg of alloxan. Normal and diabetic rats were administered orally with 300, 600, 750 mg/kg/ b.w of S. latifolius. After 28 days, the animals were sacrificed for biochemical and histological studies. The body weight of the normal and diabetic rats increased significantly with S. latifolius treatment, the increase observed in the blood glucose was brought down upon treatment with S. latifolius leaf powder. The activity of ALT increased significantly with 750 mg/kg of S. latifolius leaf powder, while low dose of the plant decreased it significantly in diabetic rats. GGT activity only decreased in the diabetic rats treated with 300 mg/kg of S. latifolius whereas albumin increased significantly (p<0.05) in all the groups administered S. latifolius powder relative to the untreated diabetic group. Bilirubin concentration only increased significantly (p<0.05) in the group administered 750 mg/kg of S. latifolius leaf powder. Histological changes including infiltration of the sinusoids and focal area by inflammatory cells and mild portal congestion were observed in all the groups except the normal and diabetic rats treated with 300 mg/kg of S. latifolius leaf powder. The result of the study showed that S. latifolius could only be encouraged for diabetes management only at low dose and might be hepatotoxic at high dose.

Topically Applied Cmt-2 Enhances Wound Healing in Streptozotocin Diabetic Rat Skin

1998 ◽  
Vol 12 (1) ◽  
pp. 144-148 ◽  
Author(s):  
N.S. Ramamurthy ◽  
A.J. Kucine ◽  
S.A. McClain ◽  
T.F. McNamara ◽  
L.M. Golub
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Chronic Wounds ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Delayed Wound Healing ◽  
Chemically Modified ◽  
Secondary Intention ◽  
Valuable Adjunct ◽  
Thickness Skin

Delayed wound healing is one of the complications of diabetes mellitus, exhibited by increased wound collagenase and decreased granulation tissues. The current study compared wound healing in normal and diabetic rats, and the effects of topically applied 1 % or 3% concentrations of chemically modified tetracycline-2 (CMT-2) on 6-mm circular full-thickness skin wounds healed by secondary intention. On day 7 after wounding, tissues were removed for biochemical analysis and histology. The wound granulation tissue hydroxyproline was less in the untreated diabetic rat with increased collagenase and gelatinase. Treating the diabetic rat wounds with 3% CMT-2 increased the wound hydroxyproline and decreased activities of gelatinase and collagenase. There was a delay in wound filling by granulation tissue in diabetic rats. In CMT-2-treated diabetic rats, the volume of granulation tissue was greater than that in untreated diabetic rats. CMT-2 appears to normalize wound healing in diabetic rats and may be a valuable adjunct in the treatment of chronic wounds.

scholarly journals A Study on Evaluation of Apoptosis and Expression of Bcl-2-Related Marker in Wound Healing of Streptozotocin-Induced Diabetic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Surya Bhan ◽  
Rahul Mitra ◽  
A. K. Arya ◽  
H. P. Pandey ◽  
K. Tripathi
Keyword(s):  
Wound Healing ◽  
Blood Sugar ◽  
Granulation Tissue ◽  
Healing Process ◽  
Late Phase ◽  
Vascular Complications ◽  
Inflammatory Cells ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Small Vessels

Uncontrolled blood sugar is a major cause of vascular complications and delayed wound healing in diabetes mellitus. During wound healing process, normally, apoptosis is responsible for events such as removal of inflammatory cells and evolution of granulation tissue into scar which occur during the late phase of wound healing. Early apoptosis can lead to abnormal wound healing by removing granulation tissue including fibroblast, endothelial cell, and small vessels. To determine the role of apoptosis in association with hyperglycemia in diabetic wound healing, apoptosis-related intracellular marker such as expression of Bcl-2 protein by immunohistochemistry and normal histology has been studied. Histological findings show higher level of apoptosis and diminished granulation tissue formation in diabetic rats wounds along with minimal expression of Bcl-2 in diabetic rats wounds when compared with nondiabetic rats wounds. It can be concluded from this study that elevated blood sugar level may be associated with increased apoptosis and the least expression of Bcl-2 protein which might cause deregulation of the wound healing processes in streptozotocin-induced diabetic rats.

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