scholarly journals High seroprevalence of bluetongue virus antibodies in goats in southeast Iran

2014 ◽  
Vol 4 ◽  
pp. S275-S278 ◽  
Author(s):  
Ali Asghar Mozaffari ◽  
Mohammad Khalili ◽  
Sina Sabahi
Keyword(s):  
Bluetongue Virus ◽  
High Seroprevalence

High seroprevalence of bluetongue virus infection in sheep flocks in West Azerbaijan, Iran

2010 ◽  
Vol 33 (3) ◽  
pp. 243-247 ◽  
Author(s):  
S. Jafari Shoorijeh ◽  
A.G. Ramin ◽  
N.J. Maclachlan ◽  
B.I. Osburn ◽  
A. Tamadon ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bluetongue Virus ◽  
High Seroprevalence ◽  
West Azerbaijan

scholarly journals Co-Circulation of Multiple Serotypes of Bluetongue Virus in Zambia

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 963
Author(s):  
Herman M. Chambaro ◽  
Michihito Sasaki ◽  
Edgar Simulundu ◽  
Isaac Silwamba ◽  
Yona Sinkala ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Bluetongue Virus ◽  
Prevention And Control ◽  
Control Strategies ◽  
Viral Disease ◽  
Sub Saharan Africa ◽  
High Seroprevalence ◽  
Economic Implications ◽  
Sub Saharan ◽  
And Control

Bluetongue (BT) is an arthropod-borne viral disease of ruminants with serious trade and socio-economic implications. Although the disease has been reported in a number of countries in sub-Saharan Africa, there is currently no information on circulating serotypes and disease distribution in Zambia. Following surveillance for BT in domestic and wild ruminants in Zambia, BT virus (BTV) nucleic acid and antibodies were detected in eight of the 10 provinces of the country. About 40% (87/215) of pooled blood samples from cattle and goats were positive for BTV nucleic acid, while one hartebeest pool (1/43) was positive among wildlife samples. Sequence analysis of segment 2 revealed presence of serotypes 3, 5, 7, 12 and 15, with five nucleotypes (B, E, F, G and J) being identified. Segment 10 phylogeny showed Zambian BTV sequences clustering with Western topotype strains from South Africa, intimating likely transboundary spread of BTV in Southern Africa. Interestingly, two Zambian viruses and one isolate from Israel formed a novel clade, which we designated as Western topotype 4. The high seroprevalence (96.2%) in cattle from Lusaka and Central provinces and co-circulation of multiple serotypes showed that BT is widespread, underscoring the need for prevention and control strategies.

scholarly journals Circulation of bluetongue virus in goats in the Karamoja region of Uganda

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Elijah N. Mulabbi ◽  
Chrisostom Ayebazibwe ◽  
Samuel Majalija ◽  
Carrie A. Batten ◽  
Christopher A.L. Oura
Keyword(s):  
Real Time ◽  
Whole Blood ◽  
Bluetongue Virus ◽  
Rna Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
High Seroprevalence ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Karamoja Region

The presence of bluetongue virus (BTV) in indigenous goats from the Karamoja region of northern Uganda was investigated. A total of 300 goats were sampled (serum and whole blood) from five districts within the Karamoja region. The samples were analysed for the presence of bluetongue (BT) antibodies using a commercial Enzyme-linked immunosorbent assay (ELISA) and for the presence of BTV viral RNA by real-time Reverse transcription polymerase chain reaction (RT-PCR), because BTV is an RNA virus. Of the 300 goats tested, 269 (90%) were positive for BTV antibodies, indicating high levels of BTV circulation within the region. Out of the 150 whole blood samples tested for the presence of the virus by real-time RT-PCR, 84 (56%) were positive for BTV RNA. This study, which is the first of its kind in Uganda, showed a high seroprevalence of BT antibodies and active circulation of BTV in a high proportion of goats in the Karamoja region.

scholarly journals Seroprevalence of Bluetongue Virus in Dairy Herds with Reproductive Problems in Sudan

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Amira Mohamed Elhassan ◽  
Mohamed Abdalla Fadol ◽  
Abdel Rahim Mohamed El Hussein
Keyword(s):  
Bluetongue Virus ◽  
Neonatal Death ◽  
Cross Sectional Study ◽  
Dairy Herds ◽  
Sectional Study ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Cross Sectional ◽  
Factors Associated ◽  
Potential Risk Factors

The objectives of this cross-sectional study were to determine the seroprevalence of blue tongue virus (BTV) and assess potential risk factors associated with BTV infection in dairy cattle with reproductive problems in Sudan. Serum samples were collected from a total of 784 animals from 37 herds and tested for antibodies against BTV using cELISA. A total of 663 out of 784 (84.57%) sera tested proved positive for BTV antibodies in all farms tested in Khartoum and Gazira States. The prevalence of antibodies was high in both areas being 94.32% in Gazira State and 76.62% in Khartoum State. BTV antibodies prevalence were significantly higher (P<0.000) in older animals than in younger ones. These rates were also significantly higher in the rainy season (P<0.000) and in Gazira State compared to Khartoum State. Sex also showed significant (P<0.000) differences in the seroprevalence, whereby females (74.7%) had higher level than males (9.8%). However, no significant (P>0.09) variations for BTV seroprevalence were observed between breeds. The BTV antibodies prevalence in infertility cases (86.6%) was not significantly different from that found in abortion (74.3%) or neonatal death (66.7%) cases. The high seroprevalence of BTV recorded herein calls for control strategy to be implemented.

scholarly journals Seroprevalence of bluetongue disease virus (BTV) among domestic ruminants in Kosovo and first record of BTV serotype 4 in sheep.

2019 ◽  
Vol 22 (1) ◽  
pp. 50-56
Author(s):  
N. Marku ◽  
K. Bërxholi ◽  
J. Spahiu ◽  
K. Sherifi ◽  
A. Rexhepi
Keyword(s):  
Control Strategy ◽  
Bluetongue Virus ◽  
Clinical Signs ◽  
First Record ◽  
Rt Pcr ◽  
High Seroprevalence ◽  
Global Challenge ◽  
Domestic Ruminants ◽  
Bluetongue Disease ◽  
And Control

The objective of the study was to estimate the seroprevalence and serotype of bluetongue virus (BTV) in domestic ruminants in different regions in Kosovo, in years 2014 and 2015. A total of 905 blood sera were analysed: 633 from sheep, 204 from cattle and 68 from goats, collected in 170 farms, 88 villages in 18 municipalities. All samples were analysed with c-ELISA for detection of BTV seroprevalence. From sheep with clinical signs samples were collected and were analysed with specific RT-PCR. Out of all 905 samples analysed with c-ELISA, 105 samples (11.6%) were seropositive (53 ovine, 39 bovine and 13 caprine). The 43 samples from sheep with clinical sings for bluetongue disease were confirmed by RT-PCR, and BTV-4 serotype was identified. The results indicated high seroprevalence of BTV in domestic ruminants, evidence of BTV-4 serotype in sheep, suggesting a need to strengthen national and regional scientific efforts and control strategy to meet the global challenge of this infectious disease.

Exposure of protein VP7 on the surface of bluetongue virus

1990 ◽  
Vol 48 (3) ◽  
pp. 600-601
Author(s):  
A.D. Hyatt
Keyword(s):  
Bluetongue Virus ◽  
Structural Proteins ◽  
Major Constituent ◽  
Outer Coat ◽  
Virus Particles ◽  
Antibody Recognition ◽  
The Core ◽  
The Family ◽  
Electron Microscopical ◽  
Physical Accessibility

Bluetongue virus (BTV) is the type species os the genus orbivirus in the family Reoviridae. The virus has a fibrillar outer coat containing two major structural proteins VP2 and VP5 which surround an icosahedral core. The core contains two major proteins VP3 and VP7 and three minor proteins VP1, VP4 and VP6. Recent evidence has indicated that the core comprises a neucleoprotein center which is surrounded by two protein layers; VP7, a major constituent of capsomeres comprises the outer and VP3 the inner layer of the core . Antibodies to VP7 are currently used in enzyme-linked immunosorbant assays and immuno-electron microscopical (JEM) tests for the detection of BTV. The tests involve the antibody recognition of VP7 on virus particles. In an attempt to understand how complete viruses can interact with antibodies to VP7 various antibody types and methodologies were utilized to determine the physical accessibility of the core to the external environment.

Umatilla Virus Association with Fibrils and Tubules in Cultured Cells

1980 ◽  
Vol 38 ◽  
pp. 476-477
Author(s):  
Neil M. Foster ◽  
Ruth D. Breckon
Keyword(s):  
Bluetongue Virus ◽  
Linear Array ◽  
Cultured Cells ◽  
Intimate Association ◽  
Infected Cells ◽  
Dark Staining ◽  
In Cells

Macrotubules have been described1 in cells infected with Umatilla virus (UMAV), an orbivirus for which bluetongue virus (BTV) is the protype. Macrotubules, often in linear array, were observed in the cytoplasm and in intimate association with viroplasms of infected cells. Macrotubules had outside and inside diameters of 20 and 15 nm and many had dark-staining centers with diameters similar to the interiors of the tubules. UMAV was 60 nm and the RNA core was 30 nm in diameter. This report describes the association of UMAV with macrotubules and two types of microtubules.

Cow fever – High seroprevalence of Coxiella burnetii antibodies in veterinarians associated with obstetric activity on cattle, Germany, 2009

2010 ◽  
Vol 72 (08/09) ◽  
Author(s):  
H Bernard ◽  
S Brockmann ◽  
N Kleinkauf ◽  
C Klinc ◽  
C Wagner-Wiening ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
High Seroprevalence
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