Donors left over blood as a source of genomic DNA for genetic studies

2016 ◽  
Vol 4 ◽  
pp. 25-32
Author(s):  
Siti Nazihahasma Hassan ◽  
Wan Suriana Wan Ab Rahman ◽  
Wan Muhamad Amir W Ahmad ◽  
Suharni Mohamad ◽  
Rosline Hassan ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Genetic Studies

scholarly journals Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position

2016 ◽  
Vol 48 (3) ◽  
pp. 196-201 ◽  
Author(s):  
S. M. Heffernan ◽  
L. P. Kilduff ◽  
R. M. Erskine ◽  
S. H. Day ◽  
J. S. McPhee ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Odds Ratio ◽  
Genomic Dna ◽  
Multiple Comparisons ◽  
Rugby Union ◽  
Genetic Studies ◽  
P Values ◽  
Taqman Probes ◽  
Genotype Frequencies ◽  
Caucasian Men

We aimed to quantify the ACE I/D and ACTN3 R577X (rs1815739) genetic variants in elite rugby athletes (rugby union and league) and compare genotype frequencies to controls and between playing positions. The rugby athlete cohort consisted of 507 Caucasian men, including 431 rugby union athletes that for some analyses were divided into backs and forwards and into specific positional groups: front five, back row, half backs, centers, and back three. Controls were 710 Caucasian men and women. Real-time PCR of genomic DNA was used to determine genotypes using TaqMan probes and groups were compared using χ2 and odds ratio (OR) statistics. Correction of P values for multiple comparisons was according to Benjamini-Hochberg. There was no difference in ACE I/D genotype between groups. ACTN3 XX genotype tended to be underrepresented in rugby union backs (15.7%) compared with forwards (24.8%, P = 0.06). Interestingly, the 69 back three players (wings and full backs) in rugby union included only six XX genotype individuals (8.7%), with the R allele more common in the back three (68.8%) than controls (58.0%; χ2 = 6.672, P = 0.04; OR = 1.60) and forwards (47.5%; χ2 = 11.768, P = 0.01; OR = 2.00). Association of ACTN3 R577X with playing position in elite rugby union athletes suggests inherited fatigue resistance is more prevalent in forwards, while inherited sprint ability is more prevalent in backs, especially wings and full backs. These results also demonstrate the advantage of focusing genetic studies on a large cohort within a single sport, especially when intrasport positional differences exist, instead of combining several sports with varied demands and athlete characteristics.

Loggerhead turtle eggshells as a source of maternal nuclear genomic DNA for population genetic studies

2010 ◽  
Vol 11 (1) ◽  
pp. 110-115 ◽  
Author(s):  
BRIAN M. SHAMBLIN ◽  
MARK G. DODD ◽  
KRISTINA L. WILLIAMS ◽  
MICHAEL G. FRICK ◽  
REBECCA BELL ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Population Genetic ◽  
Loggerhead Turtle ◽  
Genetic Studies

scholarly journals Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

2019 ◽  
Vol 63 (3) ◽  
pp. 391-398
Author(s):  
Marek Gehrke ◽  
Beata Blaszak ◽  
Monika Stachowiak ◽  
Izabela Szczerbal ◽  
Barbara Stefańska ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Histological Analysis ◽  
Holstein Cattle ◽  
Multiple Pregnancies ◽  
Comprehensive Description ◽  
Blood Leukocytes ◽  
Genetic Studies ◽  
Tissue Samples ◽  
Linked Gene ◽  
Healthy Siblings

Abstract Introduction A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish Holstein cows. Material and Methods Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX). Results All foetuses were classified to the groupholoacardius amorphous (anideus). Molecular analysis clearly showed that at 17 microsatellite loci, the studied amorphous foetuses had identical genotypes to the viable co-twins. Conclusion Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.

scholarly journals Some aspects of the genomic DNA isolation from lavender plants of different origin.

2020 ◽  
Author(s):  
M.S. Zagorskaya ◽  
Keyword(s):  
Genomic Dna ◽  
Sodium Acetate ◽  
Dna Isolation ◽  
Molecular Genetic ◽  
Genetic Studies ◽  
Isolation Technique ◽  
Genomic Dna Isolation ◽  
The Cost ◽  
Different Origin

The method of DNA extraction from lavender plants has some difficulties. Moreover, molecular genetic studies often use not only fresh materials but also microplants from an in vitro culture or frozen material. For all these types of samples, the DNA isolation technique was developed, which is based on the CTAB buffer, but without the use of liquid nitrogen, mercaptoethanol and sodium acetate, which, in turn, simplifies and reduces the cost of the method.

scholarly journals Extraction of high-quality genomic DNA and identification of different DNA barcoding markers for chickpea (Cicer arietinum L.)

2020 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Umavathi Saraswathi ◽  
Lakshmanan Mullainathan
Keyword(s):  
Dna Barcoding ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Library ◽  
Pcr Amplification ◽  
Slight Modification ◽  
High Quality ◽  
Universal Primer ◽  
Genetic Studies

The genetic studies of individual plants, especially self-pollinated species like chickpea need to be evaluated at the DNA level with the help of molecular markers for identifying genetic variations among the plants. High-quality DNA extraction is a prerequisite for genetic studies. Extraction of intact genomic DNA with high – molecular mass is essential for the study of many molecular biology applications like Polymerase Chain Reaction, endonuclease restriction digestion, southern blot analysis, and also for the construction of a genomic library. Several plant DNA extraction methods are available, even though the DNA isolation methods that give good yield employing both quantity and quality is quite difficult especially for self-pollinated crops like a chickpea. This work was focused on developing a standard protocol for the extraction of genomic DNA and identifying different barcoding markers. The result revealed that the CTAB extraction method with slight modification in protocol had been optimized for DNA isolation. The purified DNA, which was isolated through the CTAB method, had excellent spectral qualities and is efficiently digested by a restriction endonuclease, and is found to be more suitable for long-fragment PCR amplification. DNA barcoding is considered as a promising tool because it provides a practical and standard identification of plants. The isolated DNA sample was processed with a classical DNA barcoding approach by amplifying and sequencing with a universal primer. According to the result, among the different barcoding markers studied, the RbcL and Mat K were found to given the best result for molecular species identification in chickpea.

scholarly journals 7-Deazaguanine modifications protect phage DNA from host restriction systems

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Geoffrey Hutinet ◽  
Witold Kot ◽  
Liang Cui ◽  
Roman Hillebrand ◽  
Seetharamsingh Balamkundu ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Restriction Enzymes ◽  
Arms Race ◽  
Genetic Studies ◽  
Host Restriction ◽  
Archaeal Viruses ◽  
Phage Dna ◽  
Genome Modifications

AbstractGenome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G+), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2′-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2′-deoxy-7-amido-7-deazaguanine (dADG), 2′-deoxy-7-cyano-7-deazaguanine (dPreQ0) and 2′-deoxy-7- aminomethyl-7-deazaguanine (dPreQ1). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2′-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes.

scholarly journals Evidence for two distinct α(1,2)-fucosyltransferase genes differentially expressed throughout the rat colon

1994 ◽  
Vol 300 (3) ◽  
pp. 623-626 ◽  
Author(s):  
J P Piau ◽  
N Labarriere ◽  
G Dabouis ◽  
M G Denis
Keyword(s):  
Colon Cancer ◽  
Genomic Dna ◽  
Sequence Similarity ◽  
Colon Cancer Cell ◽  
Rat Colon ◽  
Specific Primers ◽  
Genetic Studies ◽  
Antisense Orientation ◽  
Colon Cancer Cell Lines ◽  
Blood Group Abh

Blood-group-ABH antigens are carbohydrate structures widely distributed in numerous tissues. These structures are fucosylated by an alpha(1,2)-fucosyltransferase. The occurrence of at least two alpha(1,2)-fucosyltransferase genes in the human genome has been strongly suggested by genetic studies, but only one of them has been cloned so far. Specific primers deduced from this human cDNA were used to amplify a fragment of rat genomic DNA (FTA). Screening of a rat colon cDNA library with this probe allowed us to isolate a clearly distinct, but related, cDNA clone (FTB). Both sequences showed considerable sequence similarity to the human alpha(1,2)-fucosyltransferase cDNA previously cloned. Furthermore, cells transfected with these DNA fragments in antisense orientation displayed a decreased alpha(1,2)-fucosyltransferase activity, indicating that they both correspond to fragments of alpha(1,2)-fucosyltransferase genes. Finally, differential expression of these genes was demonstrated in two rat colon-cancer cell lines and throughout the rat colon.

scholarly journals Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

2021 ◽  
Vol 43 ◽  
pp. e51983
Author(s):  
Luiz Henrique Tolentino Santos ◽  
Cibelle Santos Dias ◽  
Lucas Amorim Silveira ◽  
Messulan Rodrigues Meira ◽  
Elisa Susilene Lisboa dos Santos ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Genetic Improvement ◽  
Prosopis Juliflora ◽  
Future Research ◽  
Resistance Gene Analogs ◽  
Breeding Programs ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
The City ◽  
Selection Of

The characterization and selection of molecular markers are important for genetic pre-breeding programs since they make it possible to choose the most appropriate markers to be used in future research. Therefore, enabling the generation of subsidies for genetic-molecular studies in algabora (Prosopis juliflora (Sw.) DC). The amplification profile was characterized. It was generated from 17 pairs of RGA primers (Resistance Gene Analogs) in 20 samples of genomic DNA of P. juliflora extracted from specimens collected in the city of Itapetinga, Bahia. The amplifications were performed according to previously published laboratory routines and the amplification profiles analyzed from the photodocumentation of the electrophoresis results in 2% agarose gels. Based on the amplification profiles the primer pairs were classified as: Suitable: amplifications in the whole samples and with easy visualization; Reasonable: amplification in parts of the samples and/or difficult to visualize or Inadequate: absence of visible amplification products. Descriptive analyzes associated with the number of generated markers, percentage of polymorphism, expected heterozygosity (He) and the content of polymorphic information (PIC) were also performed. In a nutshell, 12 out of the 17 pairs of RGA primers generated amplification products with easy visualization and only two of these 12 pairs of primers were monomorphic. The percentage of polymorphism varied from 60% to 100%, He and PIC presented an average of 0.21 (ranging from 0 to 0.38) and 0.17 (ranging from 0 to 0.29), respectively. The results confirm that the RGA primers present adequate characteristics for genetic studies in P. juliflora, making it possible to prioritize 12 pairs of primers, which are subject to genetic improvement studies.

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