scholarly journals Amorphus globosus foetuses in Polish Holstein cattle: anatomical, histological, and genetic studies

2019 ◽  
Vol 63 (3) ◽  
pp. 391-398
Author(s):  
Marek Gehrke ◽  
Beata Blaszak ◽  
Monika Stachowiak ◽  
Izabela Szczerbal ◽  
Barbara Stefańska ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Histological Analysis ◽  
Holstein Cattle ◽  
Multiple Pregnancies ◽  
Comprehensive Description ◽  
Blood Leukocytes ◽  
Genetic Studies ◽  
Tissue Samples ◽  
Linked Gene ◽  
Healthy Siblings

Abstract Introduction A comprehensive description is presented of four novel cases ofamorphus globosus (ag) foetuses originating from multiple pregnancies of Polish Holstein cows. Material and Methods Four amorphic foetuses were delivered by three cows. Tissue samples were collected during autopsy, embedded in paraffin, sectioned, and stained with haematoxylin and eosin. Genomic DNA was isolated from tissue samples of abnormal foetuses and from blood leukocytes of their healthy siblings. PCR reactions were used to reveal the presence of Y-linked genes (SRY and AMELY) and an X-linked gene (AMELX). Results All foetuses were classified to the groupholoacardius amorphous (anideus). Molecular analysis clearly showed that at 17 microsatellite loci, the studied amorphous foetuses had identical genotypes to the viable co-twins. Conclusion Foetuses had monozygotic origin. Histological analysis showed a low level of development of tissues of meso- and ectodermal origin, as well as features of degrading patterns.

scholarly journals Effects of Dietary Inclusion of Bilberry and Walnut Leaves Powder on the Digestive Performances and Health of Tetra SL Laying Hens

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 823 ◽  
Author(s):  
Roua Gabriela Popescu ◽  
Sorina Nicoleta Voicu ◽  
Gratiela Gradisteanu Pircalabioru ◽  
Alina Ciceu ◽  
Sami Gharbia ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Specific Activity ◽  
Basal Diet ◽  
Intestinal Content ◽  
Final Concentration ◽  
Control Group ◽  
Villus Height ◽  
Tissue Samples ◽  
Walnut Leaves ◽  
Digestive Health

The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A total number of 90 Tetra SL hens were divided into two experimental groups (E1 and E2) and one control group (C) consisting of 30 hens each. After four weeks, 10 hens of each group were sacrificed and tissue samples and intestinal content were taken from the duodenum, jejunum, and cecum in order to perform histological, enzymatic, and microbiota analyses. In groups E1 and E2, the histological analysis showed a significant increase of villus height, resulting probably in increased absorption of nutrients in duodenum and jejunum. A decrease in the specific activity of alpha-amylase and trypsin in E1 and E2 for both duodenum and jejunum compared to the control one was also recorded. In addition, the maltase and invertase specific activity in duodenum increased, a tendency that was kept for maltase but not for invertase in jejunum. The cecal microbiota of E1 and E2 individuals was characterized by an increase of Firmicutes and Lactobacilli and a decrease of Enterobacteriaceae. In conclusion, our results indicate that bilberry and walnut leaves additives in feed may improve the health status of the poultry gastrointestinal tract.

scholarly journals Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

2008 ◽  
Vol 19 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Ana Teresa Sant'anna ◽  
Luis Carlos Spolidório ◽  
Lizeti Toledo Oliveira Ramalho
Keyword(s):  
Connective Tissue ◽  
Chronic Inflammation ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Connective Tissues ◽  
Plastic Tube ◽  
Tissue Samples ◽  
Subcutaneous Connective Tissue ◽  
Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

Two Novel Nonradioactive Polymerase Chain Reaction-Based Assays of Dried Blood Spots, Genomic DNA, or Whole Cells for Fast, Reliable Detection of Z and S Mutations in the α1-Antitrypsin Gene

1992 ◽  
Vol 38 (10) ◽  
pp. 2100-2107 ◽  
Author(s):  
B S Andresen ◽  
I Knudsen ◽  
P K Jensen ◽  
K Rasmussen ◽  
N Gregersen
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Whole Cells ◽  
Blood Spots ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Alpha 1 Antitrypsin

Abstract Two new nonradioactive polymerase chain reaction (PCR)-based assays for the Z and S mutations in the alpha 1-antitrypsin gene are presented. The assays take advantage of PCR-mediated mutagenesis, creating new diagnostic restriction enzyme sites for unambiguous discrimination between test samples from individuals who are normal, heterozygous, or homozygous for the mutations. We show that the two assays can be performed with purified genomic DNA as well as with boiled blood spots. The new assays were validated by parallel testing with a technique in which PCR is combined with allele-specific oligonucleotide (ASO) probes. In all cases tested the results obtained by the different techniques were in accordance. The new assays can be used for prenatal diagnostics and can be performed directly with boiled tissue samples. Because the new assays are easy to perform and reliable, we conclude that they are well suited for routine diagnosis.

Donors left over blood as a source of genomic DNA for genetic studies

2016 ◽  
Vol 4 ◽  
pp. 25-32
Author(s):  
Siti Nazihahasma Hassan ◽  
Wan Suriana Wan Ab Rahman ◽  
Wan Muhamad Amir W Ahmad ◽  
Suharni Mohamad ◽  
Rosline Hassan ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Genetic Studies

Controversial Issues in the Management of Multiple Pregnancies

Twin Research ◽  
2001 ◽  
Vol 4 (3) ◽  
pp. 165-167 ◽  
Author(s):  
Isaac Blickstein
Keyword(s):  
Mode Of Delivery ◽  
Controversial Issues ◽  
Multiple Pregnancies ◽  
Perinatal Medicine ◽  
Genetic Studies ◽  
Special Cases ◽  
Definition Of ◽  
Multifetal Pregnancy ◽  
Selection Of ◽  
Multifetal Pregnancy Reduction

AbstractThe management of multiple pregnancies represents a true challenge for all sub-specialties concerned with perinatal medicine. Many issues were neglected over the years merely because they were rare and therefore considered not sufficiently important to merit clinical trials. This paper discusses a personal selection of controversial issues, such as multifetal pregnancy reduction of triplets and twins, special cases in multifetal preganncy reduction, need for invasive genetic studies, management of twin-twin transfusion, discordant fetal conditions, the definition of “term” in multiples, and the controversy about the mode of delivery.

Association of CYP2B6 c.516G>T Polymorphism with Acute Lymphoblastic Leukemia Susceptibility

2014 ◽  
Vol 926-930 ◽  
pp. 1073-1076
Author(s):  
Zhong Hai Yuan ◽  
Yi Ju Hou ◽  
Chen Zhao ◽  
Yan Li
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genomic Dna ◽  
Lymphoblastic Leukemia ◽  
Control Group ◽  
Study Group ◽  
Regression Analyses ◽  
Specific Primers ◽  
Blood Leukocytes ◽  
Chi Square ◽  
Chi Square Test

Abstract:Objective: To investigate whether any association exists between genetic polymorphism in CYP2B6 c.516G>T and individual susceptibility to acute lymphoblastic leukemia (ALL). Methods: Our study group consisted of 96 ALL patients(T-ALL 17 cases, B-ALL 79 cases) and 348 unrelated healthy newborn volunteers as a control group. Genomic DNA was extracted from peripheral blood and cord blood leukocytes. We genotyped CYP2B6 c.516G>T polymorphism by use of PCR with sequence-specific primers (PCR-SSP). The data were analyzed statistically using chi-square and logistic regression analyses. Results: The frequencies of GG genotype were 74.14%, 57.29%, 29.41% and 63.29%, and GT genotype were 23.85%, 37.50%, 64.71% and 31.65%, and TT genotype were 2.01%, 5.21%, 5.88% and 5.06% in control group, ALL, T-ALL, and B-ALL cases, respectively. Chi-square test showed a significant correlation between the CYP2B6 c.516G>T polymorphism GT genotype and ALL patients (OR=2.035, 95%CI=1.249-3.313, P=0.004); and T-ALL patients (OR=6.839, 95%CI=2.309-20.252, P=0.000); whereas and B-ALL patients (OR=1.554, 95%CI=0.906-2.667, P=0.108). Conclusions: This study revealed the CYP2B6 c.516GT genotype may be a risk factor to the development of ALL, especially T-ALL.

scholarly journals Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position

2016 ◽  
Vol 48 (3) ◽  
pp. 196-201 ◽  
Author(s):  
S. M. Heffernan ◽  
L. P. Kilduff ◽  
R. M. Erskine ◽  
S. H. Day ◽  
J. S. McPhee ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Odds Ratio ◽  
Genomic Dna ◽  
Multiple Comparisons ◽  
Rugby Union ◽  
Genetic Studies ◽  
P Values ◽  
Taqman Probes ◽  
Genotype Frequencies ◽  
Caucasian Men

We aimed to quantify the ACE I/D and ACTN3 R577X (rs1815739) genetic variants in elite rugby athletes (rugby union and league) and compare genotype frequencies to controls and between playing positions. The rugby athlete cohort consisted of 507 Caucasian men, including 431 rugby union athletes that for some analyses were divided into backs and forwards and into specific positional groups: front five, back row, half backs, centers, and back three. Controls were 710 Caucasian men and women. Real-time PCR of genomic DNA was used to determine genotypes using TaqMan probes and groups were compared using χ2 and odds ratio (OR) statistics. Correction of P values for multiple comparisons was according to Benjamini-Hochberg. There was no difference in ACE I/D genotype between groups. ACTN3 XX genotype tended to be underrepresented in rugby union backs (15.7%) compared with forwards (24.8%, P = 0.06). Interestingly, the 69 back three players (wings and full backs) in rugby union included only six XX genotype individuals (8.7%), with the R allele more common in the back three (68.8%) than controls (58.0%; χ2 = 6.672, P = 0.04; OR = 1.60) and forwards (47.5%; χ2 = 11.768, P = 0.01; OR = 2.00). Association of ACTN3 R577X with playing position in elite rugby union athletes suggests inherited fatigue resistance is more prevalent in forwards, while inherited sprint ability is more prevalent in backs, especially wings and full backs. These results also demonstrate the advantage of focusing genetic studies on a large cohort within a single sport, especially when intrasport positional differences exist, instead of combining several sports with varied demands and athlete characteristics.

Loggerhead turtle eggshells as a source of maternal nuclear genomic DNA for population genetic studies

2010 ◽  
Vol 11 (1) ◽  
pp. 110-115 ◽  
Author(s):  
BRIAN M. SHAMBLIN ◽  
MARK G. DODD ◽  
KRISTINA L. WILLIAMS ◽  
MICHAEL G. FRICK ◽  
REBECCA BELL ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Population Genetic ◽  
Loggerhead Turtle ◽  
Genetic Studies
Sign in / Sign up

Export Citation Format

Share Document