Phytochemical Study and Antimicrobial Activity of Two Medicinal Plants from Al-Baha Region

2019 ◽  
Vol 35 (6) ◽  
pp. 1782-1788
Author(s):  
Abdulaziz Ali Alomari ◽  
Abdalfatah Abdalla Fadlelmula ◽  
Hassen Harzali
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Plant Species ◽  
Screening Tests ◽  
Phytochemical Study ◽  
Wide Range ◽  
Capparis Spinosa

The plant is a huge therapeutic source with enormous applications in curative industry. For new sources of antimicrobial agents, chloroform, ethyl acetate, and n–butanol extracts of two medicinal plants (Dodonaea viscose and Capparis spinosa) were prepared by liquid – liquid extraction. The plants were collected from Shuda mountain / Al-Baha region/ Saudi Arabia and then evaluated systematically. Phytochemical screening tests detect the existence of flavonoids, alkaloids, tannin, terponoids, saponnins and carbohydrates in most of the performed extracts. Antimicrobial activity was assessed against five bacterial and one fungal race. The extracts of the two plants leaves showed wide vision antibacterial activity and significant antifungal activity. The antibacterial activity (zone of inhibition) of Dodonaea viscose species varied from 30 to 18 mm, whereas of Capparis spinosa the range from 20 to 16 mm for ethyl acetate extracts, for n- butanol extracts the inhibition zone differ from 15 – 10 and 16 - 10 mm respectively. These results indicated that antimicrobial activities of plant species differ to a wide range not only between species themselves but also within the tests for antimicrobial evaluation. The current result supports the medicinal use of the leaves of these studied plants which works as an antimicrobial agent. These results compare to other studies carried out for the same plants in different countries in different environments exhibited diverse outcome in chemical constituents in the plant extracts and in their effects on tested types of micro organismswhich may have been due to a number of factors, including geographical location, season and environmental factors, and the method of extraction. This study for the two plant species was first time performed in this area of Saudi Arabia.

Phenotype microarray profiling of the antibacterial activity of red cabbage

2012 ◽  
Vol 2 (6) ◽  
pp. 212
Author(s):  
F. Abu Bakar ◽  
R. R. Hafidh ◽  
A. S. Abdulamir
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Metabolic Pathways ◽  
Functional Food ◽  
Functional Foods ◽  
Phenotype Microarray ◽  
Red Cabbage ◽  
Phenotypic Profile ◽  
Wide Range

Background: Functional food can be a potent source of wide array of biocomonents with antimicrobial activity. We investigated the antibacterial activity of red cabbage (RC) extract on Gram negative and positive ATCC strains. Most intersting, we, for the first time, explored and analysed the complete phenotypic profile of RC-treated bacteria using Omnilog Phenotype Microarray.Results: This study revealed that the phenotype microarray (PM) screen was a valuable tool in the search for compounds and their antibacterial mechanisms that can inhibit bacterial growth by affecting certain metabolic pathways. It was shown that RC exerted remarkable antibacterial effect on S. aureus and E. coli bacteria, and PM showed a wide range phenotypic profile of the exerted RC antibacterial activity. RC targeted the peptide, carbon, nutriontional assembly, and sulfur metbolic pathways altogether. The peptidoglycan synthesis pathway was inferred to be targeted by RC extract at a metabolic point different from other available cell wall-targeting drugs; these could be hot targets for the discovery of new therapy for many problematic microbes.Conclusions: Taken together, the phenotype microarray for functional food and medicinal plants can be a very useful tool for profiling their antimicrobial activity. Moreover, extracts of functional food can exert antibacterial activity by hitting a wide range of metabolic pathways, at the same time leading to very difficult condition for bacteria to rapidly develop resistance. Therefore, using functional foods or medicinal plants as such, or as extracts, can be superior on mono-targeting antibiotics if the optimal concentrations and conditions of these functional foods were sought.Key words: red cabbage, bacteria, antibacterial, phenotype microarray, Omnilog, Biolog

scholarly journals Evaluation of Antibacterial Activity and Phytochemical Constituents of Leaf Extract of Lippia adoensis

2014 ◽  
Vol 1 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Yared Wasihun ◽  
Tesfalem Adraro ◽  
Solomon Ali
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Plant Species ◽  
Bacterial Infections ◽  
Food Poisoning ◽  
Salmonella Typhi ◽  
Screening Tests ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Antibacterial Screening

There are quite large numbers of traditionally used medicinal plants that are used to treat skin disorder in the ethno medical system of Ethiopia. Medicinal plants namely L. adoensis, was screened for antibacterial activity against different strains of bacteria which are known to cause various types of skin infections and food poisoning Anti bacterial effect of the plant species was evaluated against different bacterial strains. The leaves of plant species were extracted by maceration and soxhelt extraction technique for preparation of crude and fractional extract respectively. And anti bacterial screening of different concentration of both crude and fractional extract of the plant species were determined using agar well diffusion method. The test organisms were one gram positive (S.aures) and three gram negative (Salmonella typhi, E.coli and P.aeruginosa) standard organisms. The results of the initial antibacterial screening test indicated the potential of these herbal drugs in treating bacterial infections of the skin and food poisoning. Among the different fractions (petroleum ether, chloroform, acetone and methanol) tested for antibacterial activity, the non-polar fractions were found to be more active than the polar fractions. The Phytochemical screening tests carried out on L. adoensis indicated the presence of tannins, flavonoids and saponins. Different extracts L.adoensis were showed significant antibacterial activity against the S.aueres, P.aeroginosa, E.coli and S.typhi. Hence further study is recommended to identify the specific active ingredient and potential formulation of effective antibiotic.  

Evaluation of antioxidant enzyme content, phenolic content, and antibacterial activity of Commiphora gileadensis grown in Saudi Arabia

2021 ◽  
Vol 19 (4) ◽  
pp. 329-343
Author(s):  
Yaaser Q. Almulaiky ◽  
Ammar AL-Farga
Keyword(s):  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Oral Hygiene ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Reference Drug ◽  
Wide Range ◽  
Peel Extract

Commiphora gileadensis is commonly used in Saudi Arabia for oral hygiene. A lack of data about its biological activity encouraged us to evaluate the antioxidant and antibacterial activities of its leaf and stem extracts. Ethanol, methanol, acetone and deionized water were tested as extraction solvents. 80% methanol gave the highest extracted concentrations of phenolic and flavonoid substances. The leaf and stem extracts were respectively evaluated for their radical scavenging activity with DPPH (EC50 = 3.39, and 1.06), ABTS (EC50 = 0.690, and 0.55), and peroxide scavenging activity (EC50 = 2.43, and 1.28). GC-MS identified a wide range of compounds that may be responsible for these activities of the results observed. The highest levels of chlorophyll, carotenoids, and lycopene were found in the leaf extract while level of proanthocyanidins was found in the stem peels extract. The peroxidase and catalase activities of stem peel extract were higher than those of the leaf extract. The findings showed that the leaf and stem peel extracts of C. gileadensis exhibited significant antibacterial activity against the test organisms. The minimum inhibitory concentrations for the plant extracts were compared with the standard reference drug Augmentin but the time–kill curves for the C. gileadensis extracts showed that they were less effective than Augmentin. Moreover, the stem peel extract exhibited stronger antibacterial activity than the leaf extract. In conclusion, C. gileadensis can be an important source of natural antioxidants, used as a healthy chewing stick for teeth brushing and oral hygiene purposes.

scholarly journals Phytochemical Study and Biological Activities of Two Medicinal Plants used in Burkina Faso: Lannea velutina A. Rich (Anacardiaceae) and Ximenia americana L. (Olacaceae)

2019 ◽  
pp. 1-9 ◽  
Author(s):  
Dramane Pare ◽  
Jotham Yhi-pênê N’do ◽  
Samson Guenne ◽  
Mahamadi Nikiema ◽  
Adama Hilou
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Burkina Faso ◽  
Bacterial Infections ◽  
Biological Activities ◽  
Public Health Problem ◽  
Bacterial Strains ◽  
Phytochemical Study ◽  
Ximenia Americana ◽  
Study Laboratory

Background: Bacterial infections are at the origin of a number of serious pathologies, including meningitis, bronchopneumopathies, typhoid fever and especially diarrhea, which are still a real public health problem for the child population in developing countries. In most of these countries, people use medicinal plants for primary health care. The objective of this study was to determine the content of phenolic compounds, the antioxidant and antimicrobial activity of extracts from two plants used in traditional medicine in Burkina Faso, Lannea velutina and Ximenia americana. Place and Duration of Study: Laboratory of Biochemistry and Applied Chemistry (LABIOCA). Methods: The total phenolics and flavonoid content of the ethanolic extract extracts were determined by spectrometric assay. The DPPH and FRAP method were used to measure the antioxidant capacity of the extracts. The antibacterial potential was determined on five bacterial strains. Results: The ethanolic extracts of Lannea velutina showed the best polyphenol content with 969.67±8.23 ​​mgGAE/g extract against 753.145917±66.31 mgGAE/g extract for Ximenia americana. On the DPPH radical Ximenia americana gave the best activity with a percentage inhibition of 62.32±0.17% compared to Lannea velutina. This species also gave the best reducing activity with a reducing capacity of 3.45±0.97 mmol EAA/10 g of extract. Shigella dysenteria and Staphylococcus aureus were susceptible to Lannea velutina and Ximenia americana with inhibition diameters greater than 8 mm. Conclusion: These results showed that the extract has good antioxidant potential as well as antimicrobial activity. These extracts could be used to prevent damage from oxidative stress and infections.

scholarly journals Antibacterial and Antifungal Activities of Ethiopian Medicinal Plants: A Systematic Review

2021 ◽  
Vol 12 ◽  
Author(s):  
Dereje Nigussie ◽  
Gail Davey ◽  
Takele Beyene Tufa ◽  
Malcolm Brewster ◽  
Belete Adefris Legesse ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Meta Analysis ◽  
Fungal Species ◽  
Antifungal Activities ◽  
Traditional Medicines ◽  
Antibacterial And Antifungal Activities ◽  
Wide Range ◽  
Antibacterial And Antifungal

Background: Podoconiosis and lymphatic filariasis are the most common causes of lower limb lymphoedema in the tropics. Many sufferers experience frequent painful episodes of acute bacterial infection. Plant based traditional medicines are used to treat infections in many countries and are culturally established in Ethiopia. Ethiopian medicinal plants found to have antibacterial and antifungal activities were reviewed with the aim of increasing information about the treatment of wound infections in patients with lymphoedema.Methods: This study collates data from published articles on medicinal plants with antibacterial and antifungal activities in Ethiopia. A systematic search of Scopus, EMBASE, PUBMED/MEDLINE and Google Scholar was undertaken. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines were followed. The protocol was registered on PROSPERO with registration number CRD42019127471. All controlled studies of in vitro antibacterial and antifungal activities were considered. All articles containing the descriptors published until June 28, 2019 were included. The outcome was measured as percent inhibition of microbial growth. For quality assessment of individual in vitro studies, OECD guidelines and the WHO-Good Laboratory Practice (GLP) handbook were used.Results: Seventy-nine studies met the inclusion criteria. A total of 150 plant species and three compounds had been tested against 42 species of bacteria, while 43 plant species had been tested against 22 species of fungus.Conclusion: Materials derived from several Ethiopian medicinal plants have been shown to have promising activity against a variety of bacteria and fungi. Those derived from Azadiractha indica A. Juss. and Lawsonia inerms L. are the most extensively studied against a wide range of gram-negative and positive bacteria, and fungal species.

scholarly journals Phenolic composition and antioxidant potential of different solvent extracts of the endemic Origanum elongatum (Bonnet) Emberger& Maire

2020 ◽  
Vol 10 (2) ◽  
pp. 146-154
Author(s):  
Imane Tagnaout ◽  
Hannou Zerkani ◽  
Sanae Amine ◽  
Kamal Fadili ◽  
Nadia Benhlima ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Screening Tests ◽  
Dpph Radical ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Health Disorders ◽  
Hydroethanolic Extract

Plants of the Lamiaceae family are widely used for medicinal, cosmetic and culinary purposes. Phenolic compounds such as flavonoids and tannins are the main constituents of these plants; they have a proven multidirectional biological activity. Polyphenols have exceptional antioxidant potential, and they can intervene in the prevention of many health disorders. This work aims to study the composition and antioxidant power of phenolic compounds from crude extracts and fractions of Origanum elongatum leaves. This specie was subjected to phytochemical study through precipitation, turbidity and coloring reactions to highlight their secondary metabolites qualitative composition. Polyphenolic extraction was made by Soxhlet using two solvents: methanol 70%, ethanol 70%. Then, methanol’s crude was fractionated with solvents having escalating polarity; ethyl acetate, n-butanol. The polyphenols and flavonoids contents were spectrophotometrically estimated using Folin-Ciocalteau and Aluminum Trichloride methods. The antioxidant power of crude and fractionated extracts was evaluated by diphenyl-picryl-hydrazyl (DPPH●) and iron (FRAP) tests. The screening tests showed the existence of polyphenols, mucilages, sterols and triterpenes, oses and holosides. The highest yield of extraction is obtained by 70% methanol, with an average of 32.29%. Quantitative assays indicated that the hydroethanolic extract and the ethyl acetate fraction possessed high amount of polyphenols and flavonoids compared with other fractions and subsequently exhibited a marked scavenging activity on DPPH radical (IC50 = 0,085±0,002 and 0,112±0,003 mg/ml, respectively) and high reductive ability on ferric ion assay with IC50 = 0,181±0,004 mg/ml, 0,291±0,005 mg/ml, respectively.

scholarly journals TOXICITY OF Esenbeckia pumila Pohl (Rutaceae) ON Artemia salina AND Atta sexdens rubropilosa

2019 ◽  
Vol 32 (1) ◽  
pp. 101-112
Author(s):  
Geane Karla Gonçalves Ferreira Duarte ◽  
Antônio Carlos Severo Menezes ◽  
Plínio Lázaro Faleiro Naves ◽  
Odair Correa Bueno ◽  
Renato Gomes Santos ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Plant Species ◽  
Insecticidal Activity ◽  
Crude Extract ◽  
Artemia Salina ◽  
Rank Test ◽  
Atta Sexdens ◽  
Phytochemical Study ◽  
Toxic Activity ◽  
Atta Sexdens Rubropilosa

ABSTRACT The number of studies on plant compounds with insecticidal activity has increased in recent years, and one of the primary targets of these compounds is leaf-cutter ants, which are considered the most important pests in Brazilian plantations, especially ants of the genus Atta. The objective of this study was to evaluate the toxic activity of the crude extract and fractions of the leaves of Esenbeckia pumila Pohl (Rutaceae) on Artemia salina and Atta sexdens rubropilosa and to perform a phytochemical study of this plant species. The toxicity of the extract and fractions was evaluated by determining the mean lethal concentration (LC50) on A. salina. The insecticidal activity was evaluated by feeding the ants an artificial diet containing the crude extract or fractions, and the results were analyzed using the log-rank test. The substances were isolated by chromatography, and the molecular structure was determined by spectroscopy. In the bioassay with A. salina, the ethanol extract and dichloromethane and ethyl acetate fractions were highly toxic. The analysis of survival curves indicated that the mortality rate of A. sexdens rubropilosa workers fed different fractions was higher than that of ants fed the control diet, especially the ethyl acetate fraction, with a mean survival time of 3 days and cumulative mortality of 100% on day 21 (p<0.05). The phytochemical study of this plant species allowed isolating the flavonoid rutin and a mixture of the triterpenes α-amyrin, β-amyrin, and lupeol. These results suggest the insecticidal potential of E. pumila on Atta sexdens rubropilosa.

Antibacterial Activity and Phytochemical Study of Six Medicinal Plants used in Benin

2011 ◽  
Vol 14 (7) ◽  
pp. 449-455 ◽  
Author(s):  
E. Anago ◽  
L. Lagnika ◽  
J. Gbenou ◽  
F. Loko ◽  
M. Moudachiro ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Phytochemical Study

IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

2017 ◽  
Vol 10 (4) ◽  
pp. 283
Author(s):  
Shirly Kumala ◽  
Ng Vini Aprilia ◽  
Partomuan Simanjuntak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Diffusion Method

Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity

Synthesis of new antimicrobial 4-aminosubstituted 3-nitrocoumarins

2010 ◽  
Vol 64 (3) ◽  
Author(s):  
Biljana Dekić ◽  
Vidoslav Dekić ◽  
Niko Radulović ◽  
Rastko Vukićević ◽  
Radosav Palić
Keyword(s):  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Disk Diffusion ◽  
Coumarin Derivatives ◽  
Similar Activity ◽  
In Vitro Antimicrobial Activity ◽  
Yeast Strains ◽  
Wide Range

AbstractA series of new coumarin derivatives has been synthesized by condensation of 4-chloro-3-nitrocoumarin and the appropriate arylamine and sulfonamide in ethyl acetate in the presence of triethylamine. The synthesized compounds were screened for their in vitro antimicrobial activity against thirteen strains of bacteria and three fungal/yeast strains using disk diffusion assays. They were shown to possess a wide range of activities from almost completely inactive compounds to medium active ones. (4-[(5-Chloropyridin-2-yl)amino]-3-nitro-2H-chromen-2-one) showed similar activity against Klebsiella pneumoniae as tetracycline.

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