Phenotype microarray profiling of the antibacterial activity of red cabbage

2012 ◽  
Vol 2 (6) ◽  
pp. 212
Author(s):  
F. Abu Bakar ◽  
R. R. Hafidh ◽  
A. S. Abdulamir
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Metabolic Pathways ◽  
Functional Food ◽  
Functional Foods ◽  
Phenotype Microarray ◽  
Red Cabbage ◽  
Phenotypic Profile ◽  
Wide Range

Background: Functional food can be a potent source of wide array of biocomonents with antimicrobial activity. We investigated the antibacterial activity of red cabbage (RC) extract on Gram negative and positive ATCC strains. Most intersting, we, for the first time, explored and analysed the complete phenotypic profile of RC-treated bacteria using Omnilog Phenotype Microarray.Results: This study revealed that the phenotype microarray (PM) screen was a valuable tool in the search for compounds and their antibacterial mechanisms that can inhibit bacterial growth by affecting certain metabolic pathways. It was shown that RC exerted remarkable antibacterial effect on S. aureus and E. coli bacteria, and PM showed a wide range phenotypic profile of the exerted RC antibacterial activity. RC targeted the peptide, carbon, nutriontional assembly, and sulfur metbolic pathways altogether. The peptidoglycan synthesis pathway was inferred to be targeted by RC extract at a metabolic point different from other available cell wall-targeting drugs; these could be hot targets for the discovery of new therapy for many problematic microbes.Conclusions: Taken together, the phenotype microarray for functional food and medicinal plants can be a very useful tool for profiling their antimicrobial activity. Moreover, extracts of functional food can exert antibacterial activity by hitting a wide range of metabolic pathways, at the same time leading to very difficult condition for bacteria to rapidly develop resistance. Therefore, using functional foods or medicinal plants as such, or as extracts, can be superior on mono-targeting antibiotics if the optimal concentrations and conditions of these functional foods were sought.Key words: red cabbage, bacteria, antibacterial, phenotype microarray, Omnilog, Biolog

Phytochemical Study and Antimicrobial Activity of Two Medicinal Plants from Al-Baha Region

2019 ◽  
Vol 35 (6) ◽  
pp. 1782-1788
Author(s):  
Abdulaziz Ali Alomari ◽  
Abdalfatah Abdalla Fadlelmula ◽  
Hassen Harzali
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Plant Species ◽  
Screening Tests ◽  
Phytochemical Study ◽  
Wide Range ◽  
Capparis Spinosa

The plant is a huge therapeutic source with enormous applications in curative industry. For new sources of antimicrobial agents, chloroform, ethyl acetate, and n–butanol extracts of two medicinal plants (Dodonaea viscose and Capparis spinosa) were prepared by liquid – liquid extraction. The plants were collected from Shuda mountain / Al-Baha region/ Saudi Arabia and then evaluated systematically. Phytochemical screening tests detect the existence of flavonoids, alkaloids, tannin, terponoids, saponnins and carbohydrates in most of the performed extracts. Antimicrobial activity was assessed against five bacterial and one fungal race. The extracts of the two plants leaves showed wide vision antibacterial activity and significant antifungal activity. The antibacterial activity (zone of inhibition) of Dodonaea viscose species varied from 30 to 18 mm, whereas of Capparis spinosa the range from 20 to 16 mm for ethyl acetate extracts, for n- butanol extracts the inhibition zone differ from 15 – 10 and 16 - 10 mm respectively. These results indicated that antimicrobial activities of plant species differ to a wide range not only between species themselves but also within the tests for antimicrobial evaluation. The current result supports the medicinal use of the leaves of these studied plants which works as an antimicrobial agent. These results compare to other studies carried out for the same plants in different countries in different environments exhibited diverse outcome in chemical constituents in the plant extracts and in their effects on tested types of micro organismswhich may have been due to a number of factors, including geographical location, season and environmental factors, and the method of extraction. This study for the two plant species was first time performed in this area of Saudi Arabia.

scholarly journals Medicinal plants of the family Lamiaceae as functional foods – a review  

2016 ◽  
Vol 34 (No. 5) ◽  
pp. 377-390 ◽  
Author(s):  
K. Carović-Stanko ◽  
M. Petek ◽  
M. Grdiša ◽  
J. Pintar ◽  
D. Bedeković ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Functional Foods ◽  
Complex Mixture ◽  
Food Preservation ◽  
Anticancer Activities ◽  
Wide Range ◽  
The Family ◽  
Special Complex

Historically, species of the family Lamiaceae have enjoyed a rich tradition of use for flavouring, food preservation, and medicinal purposes, due to both their curative and their preventive properties. It is well known that each species has a special, complex mixture of bioactive compounds in which each component contributes to its overall bioactivity. Their value lays in the production of a wide range of secondary metabolites with potent antibacterial, antioxidant, anti-inflammatory, antimicrobial, antiviral, and anticancer activities. This review focuses on the Lamiaceae species and their secondary metabolites encompassing a wide array of beneficial functions and their applicability as sources of functional foods. It could help in addressing specific consumer needs as healthy diet is a part of the lifestyle that maintains or improves overall health.

scholarly journals FORMULATION, CHARACTERIZATION AND DETERMINATION OF ANTIBACTERIAL ACTIVITY OF POMEGRANATE (Punicagranatum) GEL.

2015 ◽  
Vol 7 ◽  
pp. 13
Author(s):  
Afendi Dahlan ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Properties ◽  
Pathogenic Microorganisms ◽  
Wide Range ◽  
Common Skin

revious studies have shown that Punicagranatum (P. granatum) extract exhibited antimicrobial activity against a wide range of pathogenic microorganisms. Thus, the aim of study was to formulate and determine the effectiveness of antimicrobial properties of gel containing P. granatummethanol extract against selected common skin pathogens including Staphylococcus aureus, Staphylococcusepidermidis, Pseudomonas aeruginosaand Candidaalbicans.

scholarly journals DETERMINATION OF ANTIBACTERIAL ACTIVITY OF SOME IMPORTANT SPICES

2015 ◽  
Vol 3 (10) ◽  
pp. 57-64
Author(s):  
Ranganathan Kapilan
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Maximum Activity ◽  
Gram Negative Bacteria ◽  
Alcoholic Extract ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Wide Range

Wide range of plant extracts are used for medicinal purposes as they are very cheap, efficient, harmless and do not cause any side effects. Spices are parts of different plants and they add special aroma and taste to the food preparations. The aim of the study was to determine the antimicrobial activity of some important naturally grown spices against gram positive and gram negative pathogenic bacteria. Antibacterial activity of the spices was tested against gram positive bacteria Bacillus pumilus, Bacillus cereus and Staphylococcus aureus and gram negative bacteria Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa using aqueous, ethanolic, methanolic and liquid nutrient extracts. Among all the extracts tested alcoholic extracts of Cardamom (Elettaria cardamom), clove (Eugenia caryophyllus) and lemongrass (Cymbopogoncitratus) showed maximum antimicrobial activity against gram negative bacteria while alcoholic extract of Cardamom (Elettaria cardamom) and lemongrass (Cymbopogoncitratus) showed maximum activity against gram positive bacteria. All the spices tested in this study proved that they have antibacterial activity and the maximum activity index (1.39) was exhibited by the ethanol extract of cardamom against E.coli.

scholarly journals FUNCTIONAL FOODS: PROSPECTS IN UKRAINE

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
L. Kaprelyants ◽  
A. Yegorova ◽  
L. Trufkati ◽  
L. Pozhitkova
Keyword(s):  
Functional Food ◽  
Food Production ◽  
Functional Foods ◽  
Well Being ◽  
Biologically Active ◽  
Human Organism ◽  
Food Substance ◽  
Food Research ◽  
Age Related ◽  
Wide Range

Today, food is intended not only to satisfy hunger and provide necessary nutrients for human organism, but also to prevent nutrition-associated diseases and improve the mental and physical condition. The formula of food in the XXI century includes constant use of both traditional natural foods and foods with desired properties in the diet. The foods with desired properties are the functional food products, i. e. those enriched with essential food substances and micronutrients. These functional products cover a wide range of foods and ingredients with various biologically active substances responsible for their health-supporting and preventive effectiveness. This allows to lower the healthcare expenditures and support the economical development. The health and general well-being of today’s Ukrainians is influenced by many factors: rush life tempo, age-related changes in organs and tissues, heredity, unhealthy habits, stress situations etc. But the great importance of the diet and numbers and quality of products from which meals are prepared also should be kept in mind. And while we cannot change some factors, we can absolutely control others, particularly the characteristics of foods. The article presents some features of modern diet of Ukrainian population as an integral factor of health risk. Also, we show food substance consumption profiles of Ukrainian population in years 2015-2018, and the rate of functional foods on Ukrainian market in the last two years. The article is a review and highlights the necessity to develop production of functional foods based on fundamental and applied researches in food technology, nutritiology and the latest molecular biological technologies. General approaches to modern principles of functional food production technology are proposed. The role of the latest molecular biological technologies in functional food production is disclosed, especially of the proteomics, nutrigenomics and metabolomics. Important directions of functional food research are shown, they are associated with identification of new functional ingredients and increasing confidence of the consumers in such products. Motivations are proposed to stimulate the food manufactures to continue to develop the functional foods and to increase their assortment, thus providing a remarkable profit from successful products.

Vitamin B12: From Deficiency to Biotechnological Solution

2019 ◽  
Vol 15 (4) ◽  
pp. 318-326
Author(s):  
Ramona Massoud ◽  
Kianoush Khosravi-Darani ◽  
Seyed M.H. Bagheri ◽  
Amir M. Mortazavian ◽  
Sara Sohrabvandi
Keyword(s):  
Vitamin B12 ◽  
Growth Condition ◽  
Propionic Acid ◽  
Functional Food ◽  
Functional Foods ◽  
Human Food ◽  
Beneficial Effects ◽  
Wide Range ◽  
Health Promoting ◽  
B12 Production

Vitamin B12 production by using propionibacteria and enriching food to produce functional foods is an important subject for researches. Some microorganisms have the potential to produce a wide range of components that are health promoting for human. Among them Propionibacteria has been identified as an effective producer of vitamin B12 and anti-microbial compounds such as propionic acid for decades. In this study at first, the structure, health beneficial effects and properties of vitamin B12 as well as scaled up production of vitamin are mentioned. Then biotechnological strategy is described as a solution to overcome vitamin deficiency and production of functional food. Finally, the specification of propionibacteria and its growth condition as well as bacterium ability to produce some other interesting metabolite in human food as byproduct are discussed.

scholarly journals Antibacterial Activity of Plant Defensins

2019 ◽  
Vol 32 (5) ◽  
pp. 507-514 ◽  
Author(s):  
Andrew E. Sathoff ◽  
Deborah A. Samac
Keyword(s):  
Antimicrobial Activity ◽  
Innate Immunity ◽  
Antibacterial Activity ◽  
Host Defense ◽  
Significant Role ◽  
Higher Plants ◽  
Plant Innate Immunity ◽  
Host Defense Peptides ◽  
Plant Defensins ◽  
Wide Range

Plant defensins are antimicrobial host defense peptides expressed in all higher plants. Performing a significant role in plant innate immunity, plant defensins display potent activity against a wide range of pathogens. Vertebrate and invertebrate defensins have well-characterized antibacterial activity, but plant defensins are commonly considered to display antimicrobial activity against only fungi. In this review, we highlight the often-overlooked antibacterial activity of plant defensins. Also, we illustrate methods to evaluate defensins for antibacterial activity and describe the current advances in uncovering their antibacterial modes of action.

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

2016 ◽  
Vol 9 (5) ◽  
pp. 259 ◽  
Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Layer Chromatography ◽  
Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

scholarly journals Nigritanine as a New Potential Antimicrobial Alkaloid for the Treatment of Staphylococcus aureus-Induced Infections

Toxins ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 511 ◽  
Author(s):  
Bruno Casciaro ◽  
Andrea Calcaterra ◽  
Floriana Cappiello ◽  
Mattia Mori ◽  
Maria Loffredo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Biological Activities ◽  
Human Keratinocytes ◽  
Chemical Diversity ◽  
Bioactive Molecules ◽  
Bacterial Strains ◽  
Wide Range

Staphylococcus aureus is a major human pathogen causing a wide range of nosocomial infections including pulmonary, urinary, and skin infections. Notably, the emergence of bacterial strains resistant to conventional antibiotics has prompted researchers to find new compounds capable of killing these pathogens. Nature is undoubtedly an invaluable source of bioactive molecules characterized by an ample chemical diversity. They can act as unique platform providing new scaffolds for further chemical modifications in order to obtain compounds with optimized biological activity. A class of natural compounds with a variety of biological activities is represented by alkaloids, important secondary metabolites produced by a large number of organisms including bacteria, fungi, plants, and animals. In this work, starting from the screening of 39 alkaloids retrieved from a unique in-house library, we identified a heterodimer β-carboline alkaloid, nigritanine, with a potent anti-Staphylococcus action. Nigritanine, isolated from Strychnos nigritana, was characterized for its antimicrobial activity against a reference and three clinical isolates of S. aureus. Its potential cytotoxicity was also evaluated at short and long term against mammalian red blood cells and human keratinocytes, respectively. Nigritanine showed a remarkable antimicrobial activity (minimum inhibitory concentration of 128 µM) without being toxic in vitro to both tested cells. The analysis of the antibacterial activity related to the nigritanine scaffold furnished new insights in the structure–activity relationships (SARs) of β-carboline, confirming that dimerization improves its antibacterial activity. Taking into account these interesting results, nigritanine can be considered as a promising candidate for the development of new antimicrobial molecules for the treatment of S. aureus-induced infections.

scholarly journals Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 257 ◽  
Author(s):  
Mickymaray
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Bioactive Compounds ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Pharmacological Agents ◽  
Antibiotic Resistant ◽  
Diverse Range ◽  
Traditional Medicinal Plants ◽  
Wide Range

Traditional medicinal plants have been cultivated to treat various human illnesses and avert numerous infectious diseases. They display an extensive range of beneficial pharmacological and health effects for humans. These plants generally synthesize a diverse range of bioactive compounds which have been established to be potent antimicrobial agents against a wide range of pathogenic organisms. Various research studies have demonstrated the antimicrobial activity of traditional plants scientifically or experimentally measured with reports on pathogenic microorganisms resistant to antimicrobials. The antimicrobial activity of medicinal plants or their bioactive compounds arising from several functional activities may be capable of inhibiting virulence factors as well as targeting microbial cells. Some bioactive compounds derived from traditional plants manifest the ability to reverse antibiotic resistance and improve synergetic action with current antibiotic agents. Therefore, the advancement of bioactive-based pharmacological agents can be an auspicious method for treating antibiotic-resistant infections. This review considers the functional and molecular roles of medicinal plants and their bioactive compounds, focusing typically on their antimicrobial activities against clinically important pathogens.

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