scholarly journals Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Risha Pratiwi ◽  
Yati Nurlaeni
Keyword(s):  
Anticancer Drug ◽  
Botanic Gardens ◽  
Class Iii ◽  
Anticancer Activities ◽  
Living Plant ◽  
Toona Sinensis ◽  
Acacia Farnesiana ◽  
Anticancer Properties ◽  
Plant Collection ◽  
Ic50 Values

Abstract. Pratiwi RA, Nurlaeni Y. 2020. Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties. Biodiversitas 21: 5186-5229. Cancer is a life-threatening disease worldwide. One approach to developing effective treatment in fighting cancerous cells is to obtain anticancer drug candidates from natural resources, such as plants. This study aimed to inventory and categorize plant collections in Cibodas Botanic Gardens (CBG), West Java, Indonesia that has anticancer properties in a detailed and comprehensive manner. Literature research was conducted in international scientific databases using several keywords expressing anticancer properties to produce list of plant species potential for anticancer. The results of this research were then cross-checked with the plant collection database of CBG. List of plants exhibits anticancer activities were then categorized based on the IC50 values (an indicator of cytotoxicity). Our result showed 291 species from 90 families of CBG plant collection harbor anticancer properties. Among them, 93, 100, 36, and 62 species have IC50 values under Class I (strong), Class II (moderate), Class III (inactive), and Class IV (insufficient IC50 data), respectively. The families with the highest number of potential anticancer plants are Lauraceae, Leguminosae, Meliaceae, Myrtaceae, Moraceae, Cupressaceae, Asparagaceae, Euphorbiaceae, Compositae, Clusiaceae, Lamiaceae, Apocynaceae, Adoxaceae, Amaryllidaceae, and Elaeocarpaceae. Species that have strong anticancer activities include Acacia farnesiana, Aglaia edulis, A. elliptica, A. silvestris, Artocarpus elasticus, Bauhinia strychnifolia, Buxus microphylla, Calophyllum soulattri, Cerbera manghas, Cocculus orbiculatus, Cryptocarya chinensis, C. konishii, C. laevigata, Dalbergia parviflora, Diospyros discolor, Erythrina abyssinica, Etlingera elatior, Ficus fistulosa, Garcinia x mangostana, Hemerocallis fulva, Jatropha gossypiifolia, Panax ginseng, Podocarpus macrophyllus, Psidium cattleianum, Sansevieria ehrenbergii, Tacca chantrieri, Toona sinensis, Viburnum odoratissimum, and V. Sambucinum. Even Serenoa repens and Taxus sumatrana contain active compounds that have been commercialized as anticancer drugs. The data resulted from this study can serve as baseline information for further research in drug discovery and development for anticancer treatments using living plant specimens collected in CBG. CBG has a great prospect of medicinal plants that require further studies for formulating anticancer drug as an alternative natural resource.

scholarly journals Improved Anticancer Activities of a New Pentafluorothio-Substituted Vorinostat-Type Histone Deacetylase Inhibitor

2021 ◽  
Vol 14 (12) ◽  
pp. 1319
Author(s):  
Nils Goehringer ◽  
Yayi Peng ◽  
Bianca Nitzsche ◽  
Hannah Biermann ◽  
Rohan Pradhan ◽  
...  
Keyword(s):  
Anticancer Drugs ◽  
Histone Deacetylases ◽  
Parent Compound ◽  
Hdac Inhibitors ◽  
Drug Candidate ◽  
Anticancer Activities ◽  
Drug Candidates ◽  
Anticancer Properties ◽  
Ic50 Values ◽  
Potent Drug

The development of new anticancer drugs is necessary in order deal with the disease and with the drawbacks of currently applied drugs. Epigenetic dysregulations are a central hallmark of cancerogenesis and histone deacetylases (HDACs) emerged as promising anticancer targets. HDAC inhibitors are promising epigenetic anticancer drugs and new HDAC inhibitors are sought for in order to obtain potent drug candidates. The new HDAC inhibitor SF5-SAHA was synthesized and analyzed for its anticancer properties. The new compound SF5-SAHA showed strong inhibition of tumor cell growth with IC50 values similar to or lower than that of the clinically applied reference compound vorinostat/SAHA (suberoylanilide hydroxamic acid). Target specific HDAC inhibition was demonstrated by Western blot analyses. Unspecific cytotoxic effects were not observed in LDH-release measurements. Pro-apoptotic formation of reactive oxygen species (ROS) and caspase-3 activity induction in prostate carcinoma and hepatocellular carcinoma cell lines DU145 and Hep-G2 seem to be further aspects of the mode of action. Antiangiogenic activity of SF5-SAHA was observed on chorioallantoic membranes of fertilized chicken eggs (CAM assay). The presence of the pentafluorothio-substituent of SF5-SAHA increased the antiproliferative effects in both solid tumor and leukemia/lymphoma cell models when compared with its parent compound vorinostat. Based on this preliminary study, SF5-SAHA has the prerequisites to be further developed as a new HDAC inhibitory anticancer drug candidate.

TrizbenzIm, Cu-Trizbenzim and Zn-Trizbenzim as G-Quadruplex Inducing and Stabilizing Compounds on Human Telomeric Sequence and their Anticancer Properties

2021 ◽  
Vol 21 ◽  
Author(s):  
Renuga Duraisamy ◽  
Palanisamy U. Maheswari ◽  
Kadhar M.M. Sheriffa Begum ◽  
Dharmar Prabhakaran
Keyword(s):  
Telomeric Sequence ◽  
Thiazole Orange ◽  
Anticancer Activities ◽  
X Ray ◽  
Anticancer Properties ◽  
Telomere Sequence ◽  
G Quadruplex ◽  
Ic50 Values ◽  
Natural Amino Acids ◽  
Nanomolar Range

Background: The benzimidazole and their derivatives have rich biological relevance with respect to available natural amino acids and their role in protein folding and quaternary conformations. Thus the ligand trizbenzIm and their Cu(II) and Zn(II) metal complexes were prepared to induce G-quadruplex conformation even under no salt conditions with remarkable anticancer activities. Methods: The ligand N,N’,N’’-Tris-(1H-benzoimidazol-2-ylmethyl)-[1,3,5]triazine-2,4,6-triamine (trizbenzIm) and its Cu and Zn complexes (Cu-trizbenzIm, Zn-trizbenzIm) were synthesized and characterized by IR, NMR and MALDI-TOF techniques. The pure ligand and its complexes were interacted with human telomere DNA sequence d(TTAGGG), HTelo8and HTelo20and the interactions were followed by circular dichroism spectroscopy, FID assay and molecular docking techniques.The compounds were tested for anticancer activity towards selected cell lines. Results: All the three compounds stabilized the HTelo8 and HTelo20 in parallel and antiparallel G-quadruplex conformations with salt conditions. Under no salt conditions, the compounds induce and stabilize the G-quadruplex conformation in antiparallel topology, selectively. The pure ligand, Cu-trizbenzIm and Zn-trizbenzIm were involved in partial or classical intercalation together with some backbone interactions on the strand. The FID assay using thiazole orange intercalator clearly supports the proposed intercalation mode of binding for the three compounds, especially for the pure ligand and the Cu-complex. The MOE docking experiments using X-ray and NMR derived G-quadruplex models with the title compounds extensively support the G-quadruplex induction and stabilization of the telomere sequence by these compounds. The guanines bases involved in the G-tetrad formation interact well with the triazine and the benzimidazole part of the ligand through strong π-π interactions. The primary mode of binding is described as end stacking and intercalation of the compounds to the G-quadruplex structures. The Cu-trizbenzIm exhibited more anticancer property in comparison to the pure ligand and the Zn-trizbenzIm complex. The IC50 values were in the nanomolar range from 50 to 150nM in concentration. Conclusion: This novel self-induction of G-quadruplex is novel without the presence of any alkali metal ions.

scholarly journals Characteristics of an Exemplary Living Collection

2007 ◽  
pp. 51-61
Author(s):  
Galen Gates
Keyword(s):  
Public Access ◽  
Cultivated Plants ◽  
Science And Society ◽  
Botanic Gardens ◽  
Botanic Garden ◽  
Living Plant ◽  
Plant Exploration ◽  
Plant Collections ◽  
Plant Collection ◽  
High Diversity

The purpose of this study was to analyse the characteristics that define a plant collection. In 2002 Chicago Botanic Garden benchmarked the living plant collections in national and international botanic gardens and arboreta in seven countries. The result of the study revealed that there were twelve characteristics of an exemplary living collection. These were: I. Institutional Collections Policy and Development PlansII. High diversity (breadth in taxa and germplasm)III. Depth or areas of specialization (plant-related areas)IV. Thorough record-keepingV. Care – maintenance practicesVI. An active Verification ProgrammeVII. Plants of wild origin with cultivated plants from their introducerVIII. Taxa of conservation concernIX. Staff expertise (knowledge acquired from the building and study of the Collection)X. Public access (to view and study plants and benefit from associated Programming)XI. Plant Exploration ProgrammesXII. Relevance to science and society for multiple generations

scholarly journals Use of Half-Generation PAMAM Dendrimers (G0.5–G3.5) with Carboxylate End-Groups to Improve the DACHPtCl2 and 5-FU Efficacy as Anticancer Drugs

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2924
Author(s):  
Cláudia Camacho ◽  
Helena Tomás ◽  
João Rodrigues
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Anticancer Drug ◽  
Water Solubility ◽  
Cancer Cell Lines ◽  
Pamam Dendrimers ◽  
Binding Assays ◽  
End Groups ◽  
Ic50 Values

The DACHPtCl2 compound (trans-(R,R)-1,2-diaminocyclohexanedichloroplatinum(II)) is a potent anticancer drug with a broad spectrum of activity and is less toxic than oxaliplatin (trans-l-diaminocyclohexane oxalate platinum II), with which it shares the active metal fragment DACHPt. Nevertheless, due to poor water solubility, its use as a chemotherapeutic drug is limited. Here, DACHPtCl2 was conjugated, in a bidentate form, with half-generation PAMAM dendrimers (G0.5–G3.5) with carboxylate end-groups, and the resulting conjugates were evaluated against various types of cancer cell lines. In this way, we aimed at increasing the solubility and availability at the target site of DACHPt while potentially reducing the adverse side effects. DNA binding assays showed a hyperchromic effect compatible with DNA helix’s disruption upon the interaction of the metallodendrimers and/or the released active metallic fragments with DNA. Furthermore, the prepared DACHPt metallodendrimers presented cytotoxicity in a wide set of cancer cell lines used (the relative potency regarding oxaliplatin was in general high) and were not hemotoxic. Importantly, their selectivity for A2780 and CACO-2 cancer cells with respect to non-cancer cells was particularly high. Subsequently, the anticancer drug 5-FU was loaded in a selected metallodendrimer (the G2.5COO(DACHPt)16) to investigate a possible synergistic effect between the two drugs carried by the same dendrimer scaffold and tested for cytotoxicity in A2780cisR and CACO-2 cancer cell lines. This combination resulted in IC50 values much lower than the IC50 for 5-FU but higher than those found for the metallodendrimers without 5-FU. It seems, thus, that the metallic fragment-induced cytotoxicity dominates over the cytotoxicity of 5-FU in the set of considered cell lines.

scholarly journals Synthesis, DFT Calculations, Antiproliferative, Bactericidal Activity and Molecular Docking of Novel Mixed-Ligand Salen/8-Hydroxyquinoline Metal Complexes

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4725
Author(s):  
Badriah Saad Al-Farhan ◽  
Maram T. Basha ◽  
Laila H. Abdel Rahman ◽  
Ahmed M. M. El-Saghier ◽  
Doaa Abou El-Ezz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Docking ◽  
Metal Complexes ◽  
Wide Spectrum ◽  
Mixed Ligand ◽  
Molecular Formula ◽  
Hep G2 ◽  
Anticancer Activities ◽  
Azomethine Group ◽  
Ic50 Values

Despite the common use of salens and hydroxyquinolines as therapeutic and bioactive agents, their metal complexes are still under development. Here, we report the synthesis of novel mixed-ligand metal complexes (MSQ) comprising salen (S), derived from (2,2′-{1,2-ethanediylbis[nitrilo(E) methylylidene]}diphenol, and 8-hydroxyquinoline (Q) with Co(II), Ni(II), Cd(II), Al(III), and La(III). The structures and properties of these MSQ metal complexes were investigated using molar conductivity, melting point, FTIR, 1H NMR, 13C NMR, UV–VIS, mass spectra, and thermal analysis. Quantum calculation, analytical, and experimental measurements seem to suggest the proposed structure of the compounds and its uncommon monobasic tridentate binding mode of salen via phenolic oxygen, azomethine group, and the NH group. The general molecular formula of MSQ metal complexes is [M(S)(Q)(H2O)] for M (II) = Co, Ni, and Cd or [M(S)(Q)(Cl)] and [M(S)(Q)(H2O)]Cl for M(III) = La and Al, respectively. Importantly, all prepared metal complexes were evaluated for their antimicrobial and anticancer activities. The metal complexes exhibited high cytotoxic potency against human breast cancer (MDA-MB231) and liver cancer (Hep-G2) cell lines. Among all MSQ metal complexes, CoSQ and LaSQ produced IC50 values (1.49 and 1.95 µM, respectively) that were comparable to that of cisplatin (1.55 µM) against Hep-G2 cells, whereas CdSQ and LaSQ had best potency against MDA-MB231 with IC50 values of 1.95 and 1.43 µM, respectively. Furthermore, the metal complexes exhibited significant antimicrobial activities against a wide spectrum of both Gram-positive and -negative bacterial and fungal strains. The antibacterial and antifungal efficacies for the MSQ metal complexes, the free S and Q ligands, and the standard drugs gentamycin and ketoconazole decreased in the order AlSQ > LaSQ > CdSQ > gentamycin > NiSQ > CoSQ > Q > S for antibacterial activity, and for antifungal activity followed the trend of LaSQ > AlSQ > CdSQ > ketoconazole > NiSQ > CoSQ > Q > S. Molecular docking studies were performed to investigate the binding of the synthesized compounds with breast cancer oxidoreductase (PDB ID: 3HB5). According to the data obtained, the most probable coordination geometry is octahedral for all the metal complexes. The molecular and electronic structures of the metal complexes were optimized theoretically, and their quantum chemical parameters were calculated. PXRD results for the Cd(II) and La(III) metal complexes indicated that they were crystalline in nature.

scholarly journals Anti-Inflammatory and Anticancer Properties of Bioactive Compounds from Sesamum indicum L.—A Review

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4426 ◽  
Author(s):  
Ming-Shun Wu ◽  
Levent Bless B. Aquino ◽  
Marjette Ylreb U. Barbaza ◽  
Chieh-Lun Hsieh ◽  
Kathlia A. De Castro-Cruz ◽  
...  
Keyword(s):  
Chronic Diseases ◽  
Inflammatory Diseases ◽  
Antioxidant Properties ◽  
Sesamum Indicum ◽  
The Body ◽  
Anticancer Activities ◽  
Anticancer Properties ◽  
Pharmacological Significance ◽  
Anti Inflammatory ◽  
Natural Medicines

The use of foodstuff as natural medicines has already been established through studies demonstrating the pharmacological activities that they exhibit. Knowing the nutritional and pharmacological significance of foods enables the understanding of their role against several diseases. Among the foods that can potentially be considered as medicine, is sesame or Sesamum indicum L., which is part of the Pedaliaceae family and is composed of its lignans such as sesamin, sesamol, sesaminol and sesamolin. Its lignans have been widely studied and are known to possess antiaging, anticancer, antidiabetes, anti-inflammatory and antioxidant properties. Modern chronic diseases, which can transform into clinical diseases, are potential targets of these lignans. The prime example of chronic diseases is rheumatic inflammatory diseases, which affect the support structures and the organs of the body and can also develop into malignancies. In line with this, studies emphasizing the anti-inflammatory and anticancer activities of sesame have been discussed in this review.

scholarly journals In Vitro Anticancer Properties of Table Grape Powder Extract (GPE) in Prostate Cancer

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1804 ◽  
Author(s):  
Avinash Kumar ◽  
Melinee D’silva ◽  
Kshiti Dholakia ◽  
Anait Levenson
Keyword(s):  
Prostate Cancer ◽  
Cell Viability ◽  
Epidemiological Data ◽  
Grape Seed ◽  
Dependent Manner ◽  
Anticancer Activities ◽  
Anticancer Properties ◽  
Vegetables And Fruits ◽  
Powder Extract

Although the link between diet and cancer is complex, epidemiological data confirm that diet is a risk factor for prostate cancer and indicate a reduced prostate cancer incidence associated with a diet rich in vegetables and fruits. Because of the known protective effect of grape seed extract (GSE) against prostate cancer, we evaluated the effects of grape powder extract (GPE) on cell viability, proliferation, and metastatic capability. Importantly, we explored the possible novel mechanism of GPE through metastasis-associated protein 1 (MTA1) downregulation in prostate cancer, since our previous studies indicated resveratrol (Res)- and pterostilbene (Pter)-induced MTA1-mediated anticancer activities in prostate cancer. We found that GPE inhibited the cell viability and growth of prostate cancer cells only at high 100 μg/mL concentrations. However, at low 1.5–15 μg/mL concentrations, GPE significantly reduced the colony formation and wound healing capabilities of both DU145 and PC3M cells. Moreover, we found that GPE inhibited MTA1 in a dose-dependent manner in these cells, albeit with considerably less potency than Res and Pter. These results indicate that stilbenes such as Res and Pter specifically and potently inhibit MTA1 and MTA1-associated proteins compared to GPE, which contains low concentrations of Res and mainly consists of other flavonoids and anthocyanidins. Our findings support continued interest in GPE as a chemopreventive and anti-cancer agent against prostate cancer but also emphasize the unique and specific properties of stilbenes on MTA1-mediated anticancer effects on prostate cancer.

scholarly journals Redox Modulation at Work: Natural Phytoprotective Polysulfanes From Alliums Based on Redox-Active Sulfur

2018 ◽  
Vol 4 (5) ◽  
pp. 397-407 ◽  
Author(s):  
Awais Anwar ◽  
Emma Gould ◽  
Ryan Tinson ◽  
Javaid Iqbal ◽  
Chris Hamilton
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Therapeutic Potential ◽  
Antimicrobial Properties ◽  
Molecular Targets ◽  
Cancer Cell Lines ◽  
Special Focus ◽  
Anticancer Activities ◽  
Comprehensive Overview ◽  
Anticancer Properties

Abstract Purpose of review This article provides a brief overview of natural phytoprotective products of allium with a special focus on the therapeutic potential of diallyl polysulfanes from garlic, their molecular targets and their fate in the living organisms. A comprehensive overview of antimicrobial and anticancer properties of published literature is presented for the reader to understand the effective concentrations of polysulfanes and their sensitivity towards different human pathogenic microbes, fungi, and cancer cell lines. Recent findings The article finds polysulfanes potentials as new generation novel antibiotics and chemo preventive agent. The effective dose rates of polysulfanes for antimicrobial properties are in the range of 0.5–40 mg/L and for anticancer 20–100 μM. The molecular targets for these redox modulators are mainly cellular thiols as well as inhibition and/or activation of certain cellular proteins in cancer cell lines. Summary Antimicrobial and anticancer activities of polysulfanes published in the literature indicate that with further development, they could be promising candidates for cancer prevention due to their selectivity towards abnormal cells.

scholarly journals A Nitrocarbazole as a New Microtubule-Targeting Agent in Breast Cancer Treatment

2021 ◽  
Vol 11 (19) ◽  
pp. 9139
Author(s):  
Maria Stefania Sinicropi ◽  
Cinzia Tavani ◽  
Camillo Rosano ◽  
Jessica Ceramella ◽  
Domenico Iacopetta ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Breast Cancer Cell Lines ◽  
Cellular Functions ◽  
Anticancer Properties ◽  
Cycle Arrest ◽  
In Silico Studies ◽  
Ic50 Values ◽  
Mcf 7

Breast cancer is still considered a high-incidence disease, and numerous are the research efforts for the development of new useful and effective therapies. Among anticancer drugs, carbazole compounds are largely studied for their anticancer properties and their ability to interfere with specific targets, such as microtubule components. The latter are involved in vital cellular functions, and the perturbation of their dynamics leads to cell cycle arrest and subsequent apoptosis. In this context, we report the anticancer activity of a series of carbazole analogues 1–8. Among them, 2-nitrocarbazole 1 exhibited the best cytotoxic profile, showing good anticancer activity against two breast cancer cell lines, namely MCF-7 and MDA-MB-231, with IC50 values of 7 ± 1.0 and 11.6 ± 0.8 μM, respectively. Furthermore, compound 1 did not interfere with the growth of the normal cell line MCF-10A, contrarily to Ellipticine, a well-known carbazole derivative used as a reference molecule. Finally, in vitro immunofluorescence analysis and in silico studies allowed us to demonstrate the ability of compound 1 to interfere with tubulin organization, similarly to vinblastine: a feature that results in triggering MCF-7 cell death by apoptosis, as demonstrated using a TUNEL assay.

scholarly journals Propagation of Dry Habitat Fern Species Using Spore Collections from Historic Herbarium Specimens

2011 ◽  
pp. 43-54
Author(s):  
Gunnar Ovstebo
Keyword(s):  
Royal Botanic Garden ◽  
Herbarium Specimens ◽  
Botanic Garden ◽  
Spore Viability ◽  
Living Plant ◽  
Herbarium Material ◽  
Plant Collection ◽  
Fern Species ◽  
Herbarium Collections ◽  
Horticultural Research

Spores sourced from historic herbarium specimens have been used to introduce wild-collected material to the Royal Botanic Garden Edinburgh (RBGE) living plant collection. The ability of dry habitat ferns to maintain spore viability for prolonged periods makes it possible to grow plants from the historically important RBGE herbarium collections. The factors that affect the ability of spores to germinate from herbarium collections are described. Three fern species from the Pteridaceae – Actiniopteris semiflabellata, Anogramma leptophylla and Aleuritopteris scioana – which were not previously in cultivation at RBGE were germinated from herbarium material of different ages. Germination was observed from all three species. Plants produced in this experiment were accessed into the RBGE living plant collection for future horticultural research and germination trials.

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