The growth and reserpine content of callus culture of Rauvolfia verticillata on the variation of sucrose concentration in MS medium

2007 ◽  
Vol 5 (1) ◽  
pp. 38-46
Author(s):  
IRMAWATI IRMAWATI ◽  
SOLICHATUN SOLICHATUN ◽  
ENDANG ANGGARWULAN
Keyword(s):  
Callus Culture ◽  
Quantitative Data ◽  
Sucrose Concentration ◽  
Culture Method ◽  
Dry Weight ◽  
Ms Medium ◽  
Fresh Weight ◽  
Significance Level ◽  
Randomized Design ◽  
Two Stages

Irmawati, Solichatun, Anggarwulan E. 2007. The growth and reserpine content of callus culture of Rauvolfia verticillata on the variation of sucrose concentration in MS medium. Biofarmasi 5: 38-46. The aim of this research was to study the effect of the variation of sucrose concentration on the growth and reserpine content on callus culture of Rauvolfia verticillata (Lour.) Baillon. The research was conducted with callus culture method consisted of two stages. The first stage was callus initiation to induce callus from leaf explant of R. verticillata, and the second stage was the reserpine production on treatment medium. This research used a Completely Randomized Design (CRD) by one factor, i.e. the variation of sucrose concentration. The sucrose concentration consisted of five levels, i.e. 0 g/L, 10 g/L, 20 g/L, 30 g/L and 40 g/L, each concentration in five replicates. The collected data consisted of qualitative and quantitative data. The qualitative data, callus morphology, was presented descriptively. The quantitative data, included fresh weight callus, dry weight callus and reserpine content, were analyzed by using ANOVA and followed by DMRT at 5% significance level. The result of research showed that the variation of sucrose concentration influenced fresh weight callus, dry weight callus and reserpine content. The increasing of sucrose concentration tended to raise callus growth, which could be seen from the fresh and dry weight callus. The highest fresh weight callus was found in medium with sucrose concentration of 20 g/L, while the highest dry weight callus was found in medium with sucrose concentration of 40 g/L. The increasing of sucrose concentration until 30 g/L raised reserpine content, but the sucrose concentration over 30 g/L decreased the reserpine content.

scholarly journals RESPON PERTUMBUHAN TANAMAN SENGON (PARASERIANTHES FALCATARIA) TERHADAP PEMBERIAN AMELIORAN PADA MEDIA CAMPURAN TANAH PASCATAMBANG BATU KAPUR

2021 ◽  
Vol 9 (3) ◽  
pp. 262
Author(s):  
Alawiyah Alawiyah ◽  
Slamet Budi Yuwono ◽  
Melya Riniarti ◽  
Dermiyati Dermiyati ◽  
Christine Wulandari
Keyword(s):  
Growth Response ◽  
Land Reclamation ◽  
Dry Weight ◽  
Fresh Weight ◽  
Significance Level ◽  
Mining Soil ◽  
Shoot Dry Weight ◽  
Randomized Design ◽  
Significant Difference ◽  
Mining Law

Open mining activities have caused soil damage, water pollution, air pollution, and damage to vegetation. Limestone post mining is a critical area that must be rehabilitated to be productive again. This problems can be fixed by doing land reclamation activities which mandated in the Mining Law Number 4, 2009. The success of land reclamation is largely determined by the choice of plant species and the provision of appropriate soil enhancers (ameliorant). This study was aimed to analyze the growth response of sengon plants on giving ameliorant in mixed limestone post mining soil. This study was designed in completely randomized design for 7 treatments and 3 replications used combination of ameliorant: P0 (without ameliorant); P1 (AH 4 kg ha-1); P2 (BFA 350 kg ha-1); P3 (FMA 500 spores plant-1); P4 (the combination of AH and FMA); P5 (the combination of BFA and FMA); and P6 (the combination of AH, BFA, and FMA). Data were processed by analysis of variance at the 0,05 significance level followed by the Least Significant Difference test. The results showed that the best growth response of sengon on giving ameliorant in mixed limestone post mining soil was P1 treatment (humic acid material) which significantly increased root length, root volume, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight in sengon plants. The addition of ameliorant can be recommended to improve the quality of revegetation plants

scholarly journals Callus Production Protocol for Aegle marmelos (L.) Corr.: As a Tool for Extraction of Secondary Metabolites

2020 ◽  
Author(s):  
D.L.C.K. Fonseka ◽  
H.N. Aluthgamage
Keyword(s):  
Secondary Metabolites ◽  
Seed Coat ◽  
Callus Induction ◽  
Dry Weight ◽  
Ms Medium ◽  
Aegle Marmelos ◽  
Fresh Weight ◽  
Randomized Design ◽  
Optimized Protocol ◽  
Callus Production

Background: Aegle marmelos (L.) Corr. is an important medicinal and a fruit tree belongs to the family Rutaceae possessing numerous valuable secondary metabolites. The growing commercial importance for secondary metabolites has led to a great demand in the pharmaceutical industry in recent years. Therefore, an efficient callus production protocol was developed as a tool for extracting valuable secondary metabolites from Aegle marmelos.Methods: For seeds, callus induction was observed under three conditions as with seed coat, after removing seed coat and split into two halves after removing seed coat. For callus multiplication, 1cm2 pieces of initiated calli were used. These explants were established in MS medium supplemented with combinations of 2, 4 D either with BAP or Kinetin. All experiments were arranged according to the completely randomized design (CRD) with 20 replicates at the Tissue Culture Laboratory, Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka, for a period of 1 year. Percentage of fungal and bacterial contaminations and percentage of bleached explants were observed to select the best explant/s. Percentage of responded explants were observed to select the best condition for callus induction and quality of callus. Growth of callus was observed visually by giving a score. Best hormonal combination for callus multiplication was observed as fresh weight and dry weight of callus produced under each treatment.Result: High quality callus with higher growth was observed in all combinations of BAP and 2, 4 D tested: ranging from 0.5 mgL-1 to 1.5 mgL-1 BAP and 1.0 mgL-1 to 2.0 mgL-1 2,4 D in Murashige and Skoog (MS) medium. Initiated calli were further multiplied in MS medium supplemented with 2,4 D combined with either BAP or Kinetin. Highest amount of callus biomass was recorded in the MS medium with 0.5 mgL-1 2, 4 D and 1.0 mgL-1 Kinetin (132.58 gL-1 fresh weight). The optimized protocol could be used to produce higher amount of callus in order to extract secondary metabolites from Aegle marmelos (L).

scholarly journals Effect of Different Strenght of Medium on Germination and Seedling Growth of Tomato and Sucrose Effect on Biomass of Tomato Callus

Jurnal Biota ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 12-18
Author(s):  
Arkan Setiaji
Keyword(s):  
Seedling Growth ◽  
Plant Cell Culture ◽  
Sucrose Concentration ◽  
Dry Weight ◽  
Ms Medium ◽  
Fresh Weight ◽  
Cytoprotective Activity ◽  
Callus Production ◽  
Sucrose Effect ◽  
Nutraceutical Products

Bioactive compounds of plant cell culture extracts are currently being looked at for its potential for the development of nutraceutical products. Tomato callus is known to have cytoprotective activity. The availability for explants and its callus production are two important things. This paper reports the effect of different strengths of medium on seed germination and seedling growth of tomato cv. Permata and sucrose effects on their biomass callus. Seeds were grown on 4 kind of germination media. Hypocotyl were taken as explants for callus induction in MS medium supplemented by 2.0 mg/l BA and 0.2 mg/l NAA, 3% sucrose, and subcultured after 20 days on medium with 6 variations of sucrose. The highest values ​​for tomato hypocotyl’s height were obtained from the ½ and ¼ MS, while the highest fresh weight was obtained from the MS 0. Both the fresh weight and dry weight of the callus increased along with increasing sucrose concentration. The explant age and biomass need to be a concern regarding what kind of callus that will be produced.  Application of 5% sucrose concentration is recommended for callus production in this study.

scholarly journals Effect of N-methyl-N-nitro-N-nitrosoguanidine (NTG) on callus induction and survival rate on mature embryos of beans (Phaseolus vulgaris)

2009 ◽  
Vol 3 (2) ◽  
pp. 91-98
Author(s):  
Sattar A. Shlahi ◽  
Zahra N. Hashim Al- Hattab
Keyword(s):  
Survival Rate ◽  
Callus Induction ◽  
Indole Acetic Acid ◽  
Casein Hydrolysate ◽  
Dry Weight ◽  
Ms Medium ◽  
Fresh Weight ◽  
Benzyl Adenine ◽  
Mature Embryos ◽  
Survival Percentage

This research was conducted to study the effect of the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine on the percentage of callus induction and survival from mature beans embryos harvester cultivar. Seeds were treated with (0.2 or 0.4) millimolar of the mutagen NTG in combination with 0.0, 4 or 8% of ethanol, pH 5 ±2 0. for 24 h. Calli were induced on mature embryos by using MS medium with 0.5 mg/l of Benzyl adenine (BA), 1 mg/l Indole acetic acid (IAA) and 100 mg/l from each of Casein hydrolysate, Glycine, Asparagine, Tyrosine, and Myo-Inositol. Results showed that the hypocotyl surpassed the radical and the plume significantly in terms of survival reached 56.3%. Mutagen treatments showed asignificant effect on calli survival. Treatment with 8% Ethanol was lethal for all explants. While treatment with 0.4 mM NTG without Ethanol gaved the highest survival rate. The interaction between the treatments and the explants showed that the lowest survival percentage was which 8.8% that was for shoots treated with 0.2 mM of 4% Ethanol. Calli induced on hypocotyls treated with 0.4 mM NTG without Ethanol gave the highest fresh weight (347.2) mg while the lowest was (60) mg for calli induced on the radical treated with 0.4 mM NTG with 4% Ethanol. Moreover the highest dry weight was 22.5 mg for calli induced from hypocotyls treated with 0.4 millimolar NTG without Ethanol that was higher than the control 17.2 mg.The lowest dry weight obtained from calli induced on the radical treated with 0.4 mM NTG with 4% Ethanol was 3 mg. In conclusion the results showed that 0.4 mM NTG without Ethanol gave the highest survival rate and the highest fresh and dry weight for calli induced on the hypocotyl.

scholarly journals PEMANFAATAN PUPUK ORGANIK CAIR PAITAN (Tithonia diversifolia) PADA BERBAGAI DOSIS DAN RAGAM APLIKASINYA TERHADAP PERTUMBUHAN KAILAN (Brassica oleracea Var. Acephala)

2020 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
AHMAD TAOFIK ◽  
SOFIYA HASANI ◽  
AYU CAHYANINGTYAS ◽  
BUDY FRASETYA
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Analysis Of Variance ◽  
Growth Parameters ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Fresh Weight ◽  
Application Method ◽  
Randomized Design ◽  
Two Factors

The paitan plant contains allelopathic compounds for other plants so that the utilize of paitan plant as a source of liquid organic fertilizer (LOF) has not been widely applied. This research aimed to study the interaction between LOF dosage and appropriate application method of LOF to increase the growth of kailan plants. The research conducted at experimental garden of UIN Bandung from June-August 2016. The experimental design was a completely randomized design with two factors and replicated four times. The first factor was application methods (A), i.e. a1=direct application to the soil, and a2= spraying. The second factor was LOF dosage (T), i.e. t0: 0 ml plant-1, t1: 20 ml plant-1, t2: 40 ml plant-1, t3: 60 ml plant-1, t4: 80 ml plant-1. The growth parameters observed i.e. plant height (14, 21, 28, 35 and 42 Day after Transplanting (DAT)), leaf area 42 DAT, plant dry weight 42 DAT, and plant fresh weight 42 DAT. The data then analyzed with analysis of variance at a significant level of 5%. If the analysis of variance was significant, the Duncan multiple range test was used at α=5%. The research results showed there was no interaction between method of application with a various dosage of paitan LOF to all of the growth parameters. The application paitan LOF showed similar growth in terms of plant height, leaf area, plant dry, and plant fresh weight. The application method of paitan LOF direct to the soil or spraying to leaves can not increase kailan plant growth.

scholarly journals Postproduction Carbohydrate Levels in Pot Chrysanthemums

1991 ◽  
Vol 116 (6) ◽  
pp. 1013-1018 ◽  
Author(s):  
Susan E. Trusty ◽  
William B. Miller
Keyword(s):  
Reducing Sugars ◽  
Sucrose Concentration ◽  
Dry Weight ◽  
Soluble Carbohydrates ◽  
Fresh Weight ◽  
Plant Parts ◽  
Color Changes ◽  
Carbohydrate Levels ◽  
Reserve Carbohydrate

Postproduction changes in carbohydrate types and quantities in the leaves, stems, and inflorescences of pot chyrsanthemums [Dendranthema × gramfiflorum (Ramat.) Kitamura `Favor'] placed in interior conditions were investigated. Fructans, sucrose, glucose, and fructose were present in all plant parts. In inflorescences and leaves, an additional unidentified substance was present. All plant parts decreased in dry weight during the postproduction evaluation. This decrease was accompanied by overall reductions in total soluble carbohydrates (TSC) and starch. The appearance of leaves and stems was acceptable throughout the experiment. Leaves lost significant amounts of TSC during the first 4 days postproduction (DPP), due primarily to a 76% decrease in sucrose concentration. After 4 DPP, leaf and stem TSC remained relatively unchanged. In inflorescences, petal expansion continued through 12 DPP. Visible signs of senescence, including loss of turgor, color changes, and inrolling of petal edges were observed at 20 DPP, and by 28 DPP, the plants were determined unacceptable for consumer use. Inflorescences increased in fresh weight, but not dry weight, during petal expansion, then each decreased. Inflorescence TSC fell from 146 mg.g-1 dry weight at O DPP to 11 mg.g-1 at 28 DPP. Reducing sugars accounted for 84% of the inflorescence TSC at 4 DPP, dropping to 48% at 28 DPP. Fructan concentration decreased through 16 DPP and then remained unchanged, while starch levels rose from 25 to 34 mg·g -1 dry weight through 12 DPP, then decreased. Fractans decreased in polymerization during petal expansion. This result suggests an alternate use of fructans and starch as pools of available reserve carbohydrate during petal expansion in chrysanthemum.

scholarly journals Pengaruh Campuran Pasir dan Serat Aren pada Kailan (Brassica oleraceae) dengan Hidroponik Substrat

2014 ◽  
Vol 16 (1) ◽  
pp. 19
Author(s):  
Samanhudi Samanhudi ◽  
Dwi Hardjoko ◽  
Riandy Adhitya
Keyword(s):  
Wood Fiber ◽  
Dry Weight ◽  
Fresh Weight ◽  
Randomized Design ◽  
The Media ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Brassica Oleraceae ◽  
Media Mix ◽  
Leaf Greenness

<p>Wood fiber waste of arenga produced from starch palm industry. It is not handled properly so that could be potentially cause problems for the surrounding community. Wood fiber waste of arenga is potentially used as a medium for hydroponics. The waste can be utilized to get a new composition of hydroponic media. This study aims to determine the effect of a mixture of sand with arenga fiber waste. This research was carried out by using a mixture of sand and arenga wood fiber waste as a medium. This is an experimental study using a completely randomized design. This study aims to determine the effect of a mixture of sand and arenga fiber waste for kailan growth. The results showed that the mixture of sand and arenga wood fiber waste has no effect on kailan growth. The combination of mixtures of sand and arenga fiber waste showed no interaction. Observations on plants such as plant height, number of leaves, leaf greenness levels, the fresh weight of crop stover and stover dry weight of the plant, showed a row of the highest results in the media mix washed sand, washed sand and rice husk</p>

scholarly journals Vitrification of Crabapple, Pear, and Geum on Gellan Gum-solidified Culture Medium

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1648-1650 ◽  
Author(s):  
Steven R. Turner ◽  
Suman Singha
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Dry Weight ◽  
Pyrus Communis ◽  
Gellan Gum ◽  
The Other ◽  
Ms Medium ◽  
Fresh Weight ◽  
Malus Baccata

Shoots of `Almey' crabapple [Malus baccata (L.) Borkh. × M. pumila var. niedzwetzkyana (Dieck) Schneid.], `Seckel' pear (Pyrus communis L.), and `Mrs. Bradshaw' geum (Geum quellyon Sweet.) were cultured on Murashige and Skoog (MS) medium supplemented with 8.8 μm BA and containing 0.1% to 0.4% Gelrite. Comparative shoot proliferation and vitrification were determined on Phytagar-solidified medium. Shoot proliferation, culture fresh weight, and vitrification declined in crabapple and geum with increasing Gelrite concentration. Pear proliferation and fresh weight increased with increasing Gelrite levels, but all shoots were vitrified. There were differences in the vitrification response between pear and the other two genera. The percent dry weight of vitrified cultures on Gelrite-containing media was generally higher than that of nonvitrified cultures on medium containing Phytagar. Vitrification precludes using low Gelrite concentrations for propagating these plants. Chemical name used: N-(phenylmethyl) -1H-purin-6-amine (BA).

scholarly journals Respon Pertumbuhan Stek Anggur (Vitis vinifera L.) terhadap Pemberian Beberapa Konsentrasi Ekstrak Bawang Merah (Allium ascalonicum L.)

Akta Agrosia ◽  
2016 ◽  
Vol 19 (1) ◽  
pp. 20-27
Author(s):  
Tri Utami ◽  
Hermansyah Hermansyah ◽  
Merakati Handajaningsih
Keyword(s):  
Dry Weight ◽  
Optimum Concentration ◽  
Vitis Vinifera L ◽  
Fresh Weight ◽  
Shoot Height ◽  
Extract Concentration ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
The City

The purpose of this study was to determine the optimum concentration of shallot extract to the growth of grape cuttings grape cuttings. This study was conducted from September to December 2015 in the city of Bengkulu. This research used Completely Randomized Design with a single factor and five replications. Treatment concentrations of 0% (control), shallot extract concentration of 25%, 75% and 100%. The variables measured were the time to sprout, shoot height, the number of leaves, number of roots, fresh weight and dry weight of cuttings. The results showed that the concentration of shallot extract 100% concentration enhances the growth of grape cutting, time to sprout, and the concentration of extract 50% increase shoot height and number of leaves on grape cuttings.

scholarly journals PENGARUH KOMBINASI PUPUK ORGANIK DAN PUPUK HAYATI TERHADAP PERTUMBUHAN, PRODUKSI DAN MUTU PEGAGAN

2021 ◽  
Vol 32 (1) ◽  
pp. 23
Author(s):  
Rahma Widyastuti ◽  
Nurul Husniyati Listyana ◽  
Erri Setyo Hartanto
Keyword(s):  
Dry Weight ◽  
Growth And Yield ◽  
Stem Length ◽  
Fresh Weight ◽  
Chemical Fertilizers ◽  
Cultivation Method ◽  
Organic Cultivation ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<em>Gotu kola is one of the medicinal plants that has begun to be cultivated by the Indonesian people. It’s cultivation needs to follow the GAP to produce the qualified yield, including the use of fertilizers. Biofertilizers have been widely developed to reduce adverse effects of chemical fertilizers to the environment, thus an organic cultivation method can be an alternative. The aim of this study was to determine the effect of biofertilizer on growth and yield of gotu kola. This research was an experimental study with a completely randomized design (CRD) using 8</em> <em>planting media treatments consisting of (a) Soil + manure + dolomite; (b) Soil manure + 1 g biofertilizer; (c) Soil + dolomite + 1g biofertilizer; (d) Soil + manure + 3 biofertilizer; (e) Soil + dolomite + 3 biofertilizer; (f) Soil + manure + 5 g biofertilizer; (g)  Soil + dolomite + 5 g  biofertilizer;  and  (h)  Soil +  3 g</em> <em>biofertilizer. Parameters observed were leaf area, stem length, number of leaves, number of tillers, fresh weight, dry weight and asiaticoside content. The results showed that combination treatment of biofertilizer (1 g) with dolomite (30 g) gives better effect in increasing the number of tillers and number of leaves, while the combination of biofertilizer (5 g) and dolomite (30 g) is the best for asiaticoside levels in gotu cola.</em>

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