scholarly journals Epidemiology of clinically relevant Entamoeba spp. (E. histolytica/dispar/moshkovskii/bangladeshi): A cross sectional study from North India

2021 ◽  
Vol 15 (9) ◽  
pp. e0009762
Author(s):  
Aradhana Singh ◽  
Tuhina Banerjee ◽  
Uzma Khan ◽  
Sunit Kumar Shukla
Keyword(s):  
Molecular Detection ◽  
Cross Sectional Study ◽  
North India ◽  
Detection Methods ◽  
Cross Sectional ◽  
The People ◽  
Intestinal Infections ◽  
Routine Identification ◽  
Stool Samples ◽  
The City

Background Entamoeba infections have major impact on millions of the people worldwide. Entamoeba histolytica has long been accepted as the only pathogenic species. However, recent reports of other Entamoeba spp. in symptomatic cases have raised questions on their pathogenicity. Methodology/Principal findings Total 474 stool samples and 125 liver aspirates from patients with intestinal and extra intestinal manifestations and from community were included. Sewage samples from the hospital and the city were also included. Microscopic examination and molecular detection were performed to detect presence of E. histolytica/ dispar/ moshkovskii/ bangladeshi. The associated demographic and socioeconomic factors were statistically analyzed with the presence of Entamoeba. Microscopy detected Entamoeba spp. in 5.4% stool and 6.4% liver aspirate samples. Through nested multiplex PCR, prevalence of Entamoeba spp. in intestinal and extra-intestinal cases was 6.6% (20/301) and 86.4% (108/125) respectively and in asymptomatic population was 10.5% (13/123). Sewage samples did not show presence of any Entamoeba spp. Uneducated subjects, low economic conditions, untreated drinking water, consumption of raw vegetables and habit of not washing hands before meals were significantly associated with presence of Entamoeba spp. Conclusions E. histolytica still remains the only Entamoeba spp. in invasive extra intestinal infections. E. dispar was detected in both asymptomatic and symptomatic intestinal infections. Routine identification of Entamoeba spp. should incorporate PCR based detection methods.

Immunological and molecular detection of rotavirus genotype in calves with gastroenteritis in Diyala-Iraq

2021 ◽  
Vol 1 (1) ◽  
pp. 001-013
Author(s):  
Ammar Talib Nasser ◽  
Abdulrazak Shafiq Hasan ◽  
Amer Khazaal Saleh ◽  
Mohammad Kassem Saleh
Keyword(s):  
Molecular Detection ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Rt Pcr ◽  
Cross Sectional ◽  
Reverse Transcription Pcr ◽  
Group A ◽  
Stool Antigen ◽  
Stool Samples ◽  
Age Range

Aim: To explore the prevalence of rotavirus infection along with the molecular detection and genotyping of group A rotavirus (RVA) among bovine calves up to 5 months old in Diyala province-Iraq. Methods: This is a cross sectional study conducted in Diyala province-Iraq during the period of 2019-2020. One hundred bovine calves with age range of 1-5 months were included in the study. All were suffering acute gastroenteritis. Serum anti-rotavirus IgM and IgG plus fecal rotavirus Ag were tested for using ELISA techniques. Stool samples positive for rotavirus Ag were submitted for reverse transcription PCR (RT-PCR) for G and P genes, followed by sequencing and genotyping thereafter. Statistical analysis was done using SPSS version 25 and P values ≤ 0.05 were considered significant. Results: The positivity rate of anti-rotavirus IgM was 80% (P = 0.0001), and that of anti-rotavirus IgG was 79% (P = 0.0001). The rotavirus stool antigen was detected in 68% of calves (P = 0.01). A total of 45 stool samples which were positive for rotavirus Ag were submitted for RT-PCR; 13 (28.9%) were positive and 32 (71.1%) were negative (P = 0.084). 10 PCR positive samples were used for sequencing and genotyping and indicated that all investigated strains belonged to G1P[8] genotype. Conclusion: The current strains analyzed belonged to the G1P[8] RVA genotypes, affirming that employment of VP7 gene polymorphism accurately yielded uniform phylogenetic distances amongst investigated rotavirus strains and that there were no noticeable assortment events between human and animal rotavirus strains in Diyala province.

scholarly journals Study of Households’ Preparedness for Disasters and Emergencies in West Regions of Tehran Province, Iran

2020 ◽  
Vol 5 (4) ◽  
pp. 183-192
Author(s):  
Ahmad Jonidi Jafari ◽  
◽  
Hesam Seyedin ◽  
Masoud Baba ◽  
Shandiz Moslehi ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Health Centers ◽  
Cross Sectional ◽  
The People ◽  
Disaster Assessment ◽  
Rural Health Centers ◽  
Tehran Province ◽  
Household Preparedness ◽  
The City

Background: Disasters and emergencies are always among the major challenges and problems facing societies. By proper education and preparedness promotion for households, the effects of disasters and emergencies can be reduced. This study was performed to provide disaster assessment and education guidelines in western regions of Tehran Province in 2018. Materials and Methods: This was a cross-sectional study. For data collection, the “Disaster Assessment and Education Guidelines” provided by the Ministry of Health was used. The forms and checklists were compiled by experts of urban and rural health centers. Then, the level of household preparedness for disaster was evaluated. Results: Based on the present study in 314 centers covered, 1726803 households were evaluated. In the northwest and west Tehran health centers, Shahryar, Malard, Qods, Robat Karim, and Baharestan and all of the regions, the levels of preparedness were estimated 34.9, 24.7, 31.3, 36.2, 21.9, 24.6, 12.3, and 26.9, respectively. Conclusion: The city of Tehran is located on important faults and areas prone to floods. So Tehran is highly vulnerable to natural disasters. Because of the important role of the people and the community-based management of disasters, household readiness must be improved to reduce the burden of death, injuries, and other disastrous consequences.

scholarly journals What’s in the Kitchen: Conventional Household Practices during Minor Health Problems

2020 ◽  
pp. 104-111
Author(s):  
Yamini Bhatt ◽  
Kalpana Kulshrestha
Keyword(s):  
Rural Areas ◽  
Low Cost ◽  
Cross Sectional Study ◽  
Health Problems ◽  
North India ◽  
Age Group ◽  
Cross Sectional ◽  
The People ◽  
Cost Treatment ◽  
Long Time

Objective: The Food habits of the people are the outcome of the general beliefs and are deep-rooted in the minds of the people of any community. Home remedies coming from the kitchen are the basis of treating commonly occurring illnesses for a long time. The present study was aimed to study the long-established household practices that are followed for the cure of minor health problems in North India (Uttarakhand) and assess their usage trends over three generations. Study Design: Cross-sectional study. Methods: The study was done in three districts of Garhwal region of Uttarakhand, namely Dehradun, Tehri Garhwal and Haridwar. The respondents were categorized in three age group range as- 20-34 years, 35-55 years and above 56 years. A structured diet recall interview schedule was prepared for the collection of data. The subjects were asked about the previous and current practices followed for the cure of minor health problems. Results: A list of local food items used during various ailments like Cold/ Cough, Fever, Constipation, Vomiting, Diarrhea, Jaundice was prepared. A number of commonly used spices were used for the treatment along with few special recipe preparations. Among the age group above 56 years and 35-55 years, the percentage of respondents following traditional household practices during minor health problems and considering them better than medicine was more in rural areas while among 20-34 years of age group, the percentage was more for the urban population. It was noticed that the number of respondents following these practices slightly decreased through the generations, and are still practiced among the youngest age group interviewed (60.53% of rural and 66.67% of urban subjects). Conclusion: Documentation and validation of these household remedies is required so that they can be used for the low-cost treatment of many common ailments.

scholarly journals Immunological and molecular detection of rotavirus genotype in children with gastroenteritis in Diyala-Iraq

2021 ◽  
Vol 06 (03) ◽  
pp. 194-208
Author(s):  
Ammar Talib Nasser ◽  
Abdulrazak Shafiq Hasan ◽  
Amer Khazaal Saleh ◽  
Mohammad Kassem Saleh
Keyword(s):  
Molecular Detection ◽  
Cross Sectional Study ◽  
Conventional Pcr ◽  
High Coverage ◽  
Cross Sectional ◽  
Rotavirus A ◽  
Rotavirus Vaccines ◽  
Group A ◽  
Stool Antigen ◽  
Stool Samples

Aim: To explore the prevalence along with the molecular detection and genotyping of group A rotavirus (RVA) among children up to 5 years old complaining of gastroenteritis in Diyala province-Iraq. Methods: This is a cross sectional study conducted in Diyala province-Iraq during the period of 2019-2020. One hundred children up to 5 years old with gastroenteritis were enrolled. 54% were males and 46% were females. They were attending hospitals or primary healthcare centers. All were vaccinated with rotavirus vaccine. Blood and fecal samples were collected from each subject. Serum anti-rotavirus IgM and IgG plus fecal rotavirus Ag were tested for using ELISA techniques. Stool samples positive for rotavirus Ag were submitted for conventional PCR and for G and P genotyping thereafter. Statistical analysis was done using SPSS version 25 and P values ≤ 0.05 were considered significant. Results: The anti-rotavirus IgM and IgG positivity rates among children were 71% (P = 0.001) and 81% (P = 0.0001), respectively. Additionally, the rotavirus stool antigen was positive in 75% of the children (P = 0.001). Among 61 stool samples submitted for conventional PCR technique, the rotavirus RNA was detected in 25 (40.9%). The sequencing and genotyping investigation of 10 PCR positive products revealed that all investigated viral samples belonged to G1P[8] rotavirus A genotype. Conclusion: The current strains analyzed belonged to the G1P[8] rotavirus A genotypes, indicating high coverage of current rotavirus vaccines and affirming the importance of continuous characterization of circulating rotavirus strains and monitoring vaccine efficacy.

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

scholarly journals Gut Microbiome Composition Remains Stable in Individuals with Diabetes-Related Early to Late Stage Chronic Kidney Disease

Biomedicines ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 19
Author(s):  
Ashani Lecamwasam ◽  
Tiffanie M. Nelson ◽  
Leni Rivera ◽  
Elif I. Ekinci ◽  
Richard Saffery ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Relative Abundance ◽  
Cross Sectional Study ◽  
Early Event ◽  
Sectional Study ◽  
Cross Sectional ◽  
Microbiome Composition ◽  
Stool Samples ◽  
Late Stages

(1) Background: Individuals with diabetes and chronic kidney disease display gut dysbiosis when compared to healthy controls. However, it is unknown whether there is a change in dysbiosis across the stages of diabetic chronic kidney disease. We investigated a cross-sectional study of patients with early and late diabetes associated chronic kidney disease to identify possible microbial differences between these two groups and across each of the stages of diabetic chronic kidney disease. (2) Methods: This cross-sectional study recruited 95 adults. DNA extracted from collected stool samples were used for 16S rRNA sequencing to identify the bacterial community in the gut. (3) Results: The phylum Firmicutes was the most abundant and its mean relative abundance was similar in the early and late chronic kidney disease group, 45.99 ± 0.58% and 49.39 ± 0.55%, respectively. The mean relative abundance for family Bacteroidaceae, was also similar in the early and late group, 29.15 ± 2.02% and 29.16 ± 1.70%, respectively. The lower abundance of Prevotellaceae remained similar across both the early 3.87 ± 1.66% and late 3.36 ± 0.98% diabetic chronic kidney disease groups. (4) Conclusions: The data arising from our cohort of individuals with diabetes associated chronic kidney disease show a predominance of phyla Firmicutes and Bacteroidetes. The families Ruminococcaceae and Bacteroidaceae represent the highest abundance, while the beneficial Prevotellaceae family were reduced in abundance. The most interesting observation is that the relative abundance of these gut microbes does not change across the early and late stages of diabetic chronic kidney disease, suggesting that this is an early event in the development of diabetes associated chronic kidney disease. We hypothesise that the dysbiotic microbiome acquired during the early stages of diabetic chronic kidney disease remains relatively stable and is only one of many risk factors that influence progressive kidney dysfunction.

scholarly journals Enteroparasitosis in patients attended by the health public service: epidemiology and spatial distribution

2020 ◽  
Vol 30 (1) ◽  
pp. 34764
Author(s):  
Andressa Barros Ibiapina ◽  
Janaína Soares Leal ◽  
Pedro Ricardo Alves de Santana ◽  
Marcelo Ribeiro Mesquita ◽  
Tito Lívio da Cunha Lopes ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Entamoeba Histolytica ◽  
Ascaris Lumbricoides ◽  
Rural Areas ◽  
The Elderly ◽  
Common Species ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Intestinal Parasitosis ◽  
The City

AIMS: This research aims to determine the epidemiology and the spatial distribution of intestinal parasitosis in the city of Teresina.METHODS: A cross-sectional study was carried out based on the data of parasitological fecal exams performed in the Laboratory Raul Bacelar between January, 2014 and July, 2017. In addition to the prevalence of intestinal parasitosis and polyparasitism, we verified the association of these diseases with gender, zone and period of the year by means of the chi-squared test, whereas the relation with age was analyzed by the Mann-Kendall tests and multiple comparisons of age classes. The spatial distribution was performed using the QGIS georeferencing software.RESULTS: The prevalence of enteroparasitosis in Teresina is 17,8% with Ascaris lumbricoides being the most common species, due to the precarious sanitary conditions of the city. The prevalence of individuals with polyparasitism is 3,13%, in which an association between the species Entamoeba coli and Entamoeba histolytica/dispar was found. There was no relation between intestinal parasitosis with gender, but we verified that individuals in rural areas are more susceptible to these diseases. The species Ascaris lumbricoides and Entamoeba histolytica/dispar occur more frequently in the first and second semester, respectively. We observed that there is an apparent tendency to increase cases of E. histolytica/dispar and reduction of cases of Giardia sp. according to aging. Mapping intestinal parasitosis showed us that there is a prevalence between one and 20% in most of Teresina's neighborhoods, and Ascariasis embodies at least 40% of cases of enteroparasitosis in these neighborhoods.CONCLUSIONS: Investments in basic sanitation and new epidemiological investigations must be carried out to control intestinal parasitosis in Teresina, emphasizing that children and the elderly should be considered priority groups in these programs.

scholarly journals Survey of iodine deficiency and intestinal parasitic infections in school-going children: Bie Province, Angola

2010 ◽  
Vol 13 (9) ◽  
pp. 1314-1318 ◽  
Author(s):  
Mark Tomlinson ◽  
Vera Adams ◽  
Mickey Chopra ◽  
Pieter Jooste ◽  
Emmerentia Strydom ◽  
...  
Keyword(s):  
Iodine Deficiency ◽  
International Health ◽  
Target Population ◽  
Cross Sectional Study ◽  
Parasitic Infections ◽  
Intestinal Protozoa ◽  
Cross Sectional ◽  
Helminth Infections ◽  
Stool Samples ◽  
The Government

AbstractObjectiveTo obtain baseline pre-intervention prevalence levels of iodine deficiency and parasitic and helminth infections in school-going children in Bie Province, Angola.DesignA cross-sectional study conducted in randomly selected schools. The target population was children aged 6–10 years.SettingBie Province, Angola.SubjectsA total of 1029 children sampled, with 791 stool samples and 826 urine samples collected from twenty-four schools.ResultsWidespread severe and moderate deficiencies in iodine. Children in five schools were severely iodine deficient. All sampled schoolchildren were iodine deficient to a greater or lesser extent. In all, 80 % of all children across the twenty-four schools were infected with one or a combination of intestinal helminths and intestinal protozoa.ConclusionsThese findings have serious implications for the cognitive development of Angolan children, as well as for Angola’s development in terms of productivity and economic potential. It is strongly recommended that the provincial and national Ministries of Health, in collaboration with international health agencies, immediately plan and implement a strategy to provide sufficient iodine through iodised oil capsules and iodised salt to the iodine-deficient population. National coalitions need to be strengthened among the government, partners and salt producers. It is also recommended that all the children in schools be de-wormed for multiple helminth species at least twice a year.

Assessment of Post COVID-19 Health Problems and Its Determinants in North India: A Descriptive Cross-Sectional Study

2021 ◽  
Author(s):  
Suraj Senjam ◽  
Yatan Pal Singh Balhara ◽  
Parmeshwar Kumar ◽  
Neeraj Nischal ◽  
Souvik Manna ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Health Problems ◽  
North India ◽  
Sectional Study ◽  
Cross Sectional

Internet Addiction and the Pattern of Internet Use among Under Graduate Medical Students: A Cross- Sectional Study from North India

2022 ◽  
Author(s):  
Priyanka . ◽  
R K Pal
Keyword(s):  
Medical Students ◽  
Internet Addiction ◽  
Internet Use ◽  
Cross Sectional Study ◽  
North India ◽  
The Internet ◽  
Sectional Study ◽  
Undergraduate Medical Students ◽  
Cross Sectional ◽  
Significance Level

Introduction: Excessive use of the Internet affects the academic achievements of students. This study aimed to investigate the prevalence of Internet addiction and the pattern of Internet use among undergraduate medical students. Method: This analytical cross-sectional study conducted on 177 undergraduate medical students in batch 2016, 2017 and 2018, who were included in this study by convenience sampling method. The study tool was  Young’s Internet Addiction Test containing questions regarding the pattern of Internet use. Data entry and analysis was done by using Microsoft excel and SPSS version 21. Qualitative data were described in terms of frequency and percentage while quantitative data were expressed as mean and standard deviation. Chi square test and multiple linear regression were used to find out the relationship between various factors and Internet Addiction (IA).  The significance level was considered 0.05. Results: The mean age of the participants was 20.3 years (± 1.19), and 62 % of the subjects were males. The prevalence rate of Internet Addiction (IA) was found to be 56.5% and 42.9% of them were in mild and 13.6% were in moderate addiction category. Being male (β= -0.143, p=0.038), staying at a hostel (β= 0.167, p=0.018), not having a time preference for using the Internet (β= -0.174, p=0.012), spending more time on the Internet everyday (β= 0.201, p=0.000), being always online (β= 0.276, p=0.000) and more years of using the Internet (β= 0.175, p=0.015) were significantly related with IA in students. Conclusion: A large proportion of medical students were found to be addicted to the Internet. Therefore, this issue should be addressed immediately.

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