Analysis of the siRNA-Mediated Gene Silencing Process Targeting Three Homologous Genes Controlling Soybean Seed Oil Quality

Field and greenhouse experiments were conducted in 1995 and 1996 to determine soybean injury and weed control differences from imazamox and imazethapyr applied postemergence with a nonionic surfactant or methylated seed oil and with selected tank mixtures. Soybean injury from imazamox at 35 g ai ha−1plus either a methylated seed oil or nonionic surfactant was equal to injury from imazethapyr at 70 g ai ha−1in the greenhouse and field. Imazamox provided greater common lambsquarters control than imazethapyr in the field in 1995 and in the greenhouse. Thifensulfuron tank mixed with imazethapyr increased common lambsquarters control, while soybean response increased when thifensulfuron was tank mixed with imazamox. Common ragweed dry weight was reduced 61 to 64% from 35 g ha−1imazamox and 70 g ha−1imazethapyr in the field; however, imazamox provided greater common ragweed control than imazethapyr in the greenhouse. Tank mixtures of lactofen with imazamox or imazethapyr increased common ragweed control and resulted in greater soybean seed yield in 1996 than when imazamox and imazethapyr were applied alone; however, lactofen antagonized giant foxtail control with imazamox and imazethapyr, and antagonized common lambsquarters control with imazamox. Giant foxtail control in the greenhouse was antagonized more when acifluorfen, fomesafen, and lactofen were tank mixed with 35 g ha−1imazethapyr than with 35 g ha−1imazamox. Giant foxtail control with imazamox or imazethapyr applied alone or with diphenyl ether herbicides increased when 28% urea ammonium nitrate was added with nonionic surfactant compared with nonionic surfactant only. Imazethapyr antagonized giant foxtail control by clethodim in the field and was more antagonistic than imazamox in the greenhouse. A methylated seed oil improved common ragweed control by imazethapyr at 70 g ha−1and imazamox at 18 and 35 g ha−1, while common lambsquarters and velvetleaf control increased when a methylated seed oil was included with 18 g ha−1imazethapyr compared to nonionic surfactant in the greenhouse.

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Physicochemical Properties and Antioxidant Activity of the Seeds Oil of Two Cucurbita Species From Bejaia (Algeria): Comparative Study

Phytothérapie ◽

10.3166/phyto-2021-0280 ◽

2021 ◽

Author(s):

S. Mindjou ◽

F. Brahmi ◽

W. Belkhiri ◽

N. Adjeroud ◽

L. Benali ◽

...

Keyword(s):

Physicochemical Parameters ◽

Cucurbita Pepo ◽

Seed Oil ◽

Oil Quality ◽

Waste Product ◽

International Standards ◽

Cucurbita Moschata ◽

Phenolic Contents ◽

Methanolic Extracts

Cucurbita species are delicious, nutritious, and delightful products. Cucurbita seeds remain in large quantities as a waste product that could be valorized since they are excellent sources of oil. The aim of this study was to compare the seed oil of two Cucurbita species (Cucurbita pepo and Cucurbita moschata) harvested in Bejaia (Algeria). The oil quality was evaluated by the determination of some physicochemical parameters, and the content of phenolic compounds. The antiradical capacity of the antioxidants present in the oils was also assessed using two methods. The oil yield was 42.85% and 40.47% from the seeds of Cucurbita pepo and Cucurbita moschata, respectively. The determined physicochemical parameters were close to those defined by the international standards. The phenolic contents of the methanolic extracts of both oils were 5.53 and 4.45 mg GAE/100 g for Cucurbita moschata and Cucurbita pepo, respectively. The best anti-DPPH power was attributed to the oil of Cucurbita moschata (44.7%), while the methanolic extract of the seed oil of Cucurbita pepo showed the highest percentage (41.02%) of the ABTS•+ radical inhibition. By this study we confirmed that the Cucurbita seeds oil are highly nutritious and offer some medicinal benefits.

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Cold-pressed cactus pear seed oil: Quality and stability

Grasas y Aceites ◽

10.3989/gya.0329201 ◽

2021 ◽

Vol 72 (3) ◽

pp. e415 ◽

Cited By ~ 3

Author(s):

M. De Wit ◽

V.K. Motsamai ◽

A. Hugo

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Physicochemical Properties ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Oil Quality ◽

Cactus Pear ◽

Cold Pressed

Cold-pressed seed oil from twelve commercially produced cactus pear cultivars was assessed for oil yield, fatty acid composition, physicochemical properties, quality and stability. Large differences in oil content, fatty acid composition and physicochemical properties (IV, PV, RI, tocopherols, ORAC, % FFA, OSI and induction time) were observed. Oil content ranged between 2.51% and 5.96% (Meyers and American Giant). The important fatty acids detected were C16:0, C18:0, C18:1c9 and C18:2c9,12, with C18:2c9,12, the dominating fatty acid, ranging from 58.56-65.73%, followed by C18:1c9, ranging between 13.18-16.07%, C16:0, which ranged between 10.97 - 15.07% and C18:0, which ranged between 2.62-3.18%. Other fatty acids such as C14:0, C16:1c9, C17:0, C17:1c10, C20:0, C18:3c9,12,15 and C20:3c8,11,14 were detected in small amounts. The quality parameters of the oils were strongly influenced by oil content, fatty acid composition and physicochemical properties. Oil content, PV, % FFA, RI, IV, tocopherols, ORAC and ρ-anisidine value were negatively correlated with OSI. C18:0; C18:1c9; C18:2c9,12; MUFA; PUFA; n-6 and PUFA/SFA were also negatively correlated with OSI. Among all the cultivars, American Giant was identified as the paramount cultivar with good quality traits (oil content and oxidative stability).

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Investigation on the Extraction and Conjugate of Linoleic Acid of Purple Medic Seed

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.347-353.3337 ◽

2011 ◽

Vol 347-353 ◽

pp. 3337-3340

Author(s):

Shu Ting Qi ◽

Yu Xin Shi ◽

Ming Dong ◽

Hong Qing Wu ◽

Di Mu

Keyword(s):

Fatty Acid Composition ◽

Linoleic Acid ◽

Solvent Extraction ◽

Seed Oil ◽

Extraction Efficiency ◽

Oil Quality ◽

Total Lipids ◽

Solvent Type ◽

Effects Of Temperature

Methods currently used for the quantitative determination of total lipids and fatty acid composition in plant require solvent extraction. This study examined the solvent extraction of purple medic seed oil. Results varied with different extraction conditions. The effects of temperature, extraction time, sample size and solvent type on the extraction efficiency and oil quality were studied. The amount of extract collected was highest when ether absolute was used as a solvent for solvent extraction.

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Stability of seed oil quality traits in high and mid-oleic acid sunflower hybrids

Euphytica ◽

10.1007/s10681-013-0888-0 ◽

2013 ◽

Vol 193 (2) ◽

pp. 157-168 ◽

Cited By ~ 15

Author(s):

R. van der Merwe ◽

M. T. Labuschagne ◽

L. Herselman ◽

A. Hugo

Keyword(s):

Oleic Acid ◽

Seed Oil ◽

Oil Quality ◽

Quality Traits

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Dynamic Transcriptome Changes Related to Oil Accumulation in Developing Soybean Seeds

International Journal of Molecular Sciences ◽

10.3390/ijms20092202 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2202 ◽

Cited By ~ 3

Author(s):

Songnan Yang ◽

Long Miao ◽

Jianbo He ◽

Kai Zhang ◽

Yan Li ◽

...

Keyword(s):

Fatty Acid ◽

Candidate Genes ◽

Molecular Basis ◽

Oil Content ◽

Seed Oil ◽

Soybean Seed ◽

Soybean Seeds ◽

Seed Oil Content ◽

Oil Accumulation ◽

Expression Levels

Soybean is one of the most important oil crops in the world. Revealing the molecular basis and exploring key candidate genes for seed oil synthesis has great significance for soybean improvement. In this study, we found that oil accumulation rates and gene expression levels changed dynamically during soybean seed development. The expression levels of genes in metabolic pathways such as carbon fixation, photosynthesis, glycolysis, and fatty acid biosynthesis were significantly up-regulated during the rapid accumulation of oil in developing soybean seeds. Through weighted correlation network analysis, we identified six co-expression modules associated with soybean seed oil content and the pink module was the most positively correlated (r = 0.83, p = 7 × 10−4) network. Through the integration of differential expression and co-expression analysis, we predicted 124 candidate genes potentially affecting soybean seed oil content, including seven genes in lipid metabolism pathway, two genes involved in glycolysis, one gene in sucrose metabolism, and 12 genes belonged to transcription factors as well as other categories. Among these, three genes (GmABI3b, GmNFYA and GmFAD2-1B) have been shown to control oil and fatty acid content in soybean seeds, and other newly identified candidate genes would broaden our knowledge to understand the molecular basis for oil accumulation in soybean seeds.

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